The Experts below are selected from a list of 59139 Experts worldwide ranked by ideXlab platform
Maike Tech - One of the best experts on this subject based on the ideXlab platform.
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the ibeetle large scale rnai screen reveals gene functions for Insect Development and physiology
Nature Communications, 2015Co-Authors: Christian Schmittengel, Daniela Grossmann, Dorothea Schultheis, Van Anh Dao, Upalparna Majumdar, Nicole Troelenberg, Tobias Richter, Jonas Schwirz, Nadi Strohlein, Maike TechAbstract:Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more Insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic Development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in Insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila. Unbiased screening for Insect gene function has been largely restricted to Drosophila. Here, Schmitt-Engel et al. perform an unbiased large-scale RNAi screen in the red flour beetle Tribolium castaneumto identify putative gene functions.
Christian Schmittengel - One of the best experts on this subject based on the ideXlab platform.
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the ibeetle large scale rnai screen reveals gene functions for Insect Development and physiology
Nature Communications, 2015Co-Authors: Christian Schmittengel, Daniela Grossmann, Dorothea Schultheis, Van Anh Dao, Upalparna Majumdar, Nicole Troelenberg, Tobias Richter, Jonas Schwirz, Nadi Strohlein, Maike TechAbstract:Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more Insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic Development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in Insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila. Unbiased screening for Insect gene function has been largely restricted to Drosophila. Here, Schmitt-Engel et al. perform an unbiased large-scale RNAi screen in the red flour beetle Tribolium castaneumto identify putative gene functions.
Wenxiong Xu - One of the best experts on this subject based on the ideXlab platform.
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cathepsin l function in Insect moulting molecular cloning and functional analysis in cotton bollworm helicoverpa armigera
Insect Molecular Biology, 2006Co-Authors: Weixiong Zhang, Guren Zhang, Wenxiong XuAbstract:: Moulting is an essential process of Insect Development but little is known about cysteine proteases in the process. Here, we detail a proteolytic activity profile from fifth larval instar to new pupae of the lepidopteran Helicoverpa armigera. At fifth to sixth instar moulting, the activities were significantly higher than those in non-moulting stages, and were inhibited by the cysteine protease inhibitor, 2S, 3S-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester (E-64), or by the cathepsin L-selective inhibitor CLIK148. Further, a 1513 bp cathepsin L cDNA (Har-CL) was isolated from the H. armigera larval cuticle and epidermis layer. Har-CL gene expression, which is correlated closely with ecdysone, was higher during larval moulting. Injection of E-64 or CLIK148 resulted in delayed fifth to sixth instar moulting, suggesting an essential role for cathepsin L in larval moulting.
Van Anh Dao - One of the best experts on this subject based on the ideXlab platform.
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the ibeetle large scale rnai screen reveals gene functions for Insect Development and physiology
Nature Communications, 2015Co-Authors: Christian Schmittengel, Daniela Grossmann, Dorothea Schultheis, Van Anh Dao, Upalparna Majumdar, Nicole Troelenberg, Tobias Richter, Jonas Schwirz, Nadi Strohlein, Maike TechAbstract:Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more Insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic Development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in Insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila. Unbiased screening for Insect gene function has been largely restricted to Drosophila. Here, Schmitt-Engel et al. perform an unbiased large-scale RNAi screen in the red flour beetle Tribolium castaneumto identify putative gene functions.
Tobias Richter - One of the best experts on this subject based on the ideXlab platform.
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the ibeetle large scale rnai screen reveals gene functions for Insect Development and physiology
Nature Communications, 2015Co-Authors: Christian Schmittengel, Daniela Grossmann, Dorothea Schultheis, Van Anh Dao, Upalparna Majumdar, Nicole Troelenberg, Tobias Richter, Jonas Schwirz, Nadi Strohlein, Maike TechAbstract:Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more Insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic Development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in Insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila. Unbiased screening for Insect gene function has been largely restricted to Drosophila. Here, Schmitt-Engel et al. perform an unbiased large-scale RNAi screen in the red flour beetle Tribolium castaneumto identify putative gene functions.
