The Experts below are selected from a list of 6732 Experts worldwide ranked by ideXlab platform
Sonia Almería - One of the best experts on this subject based on the ideXlab platform.
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Cystoisospora belli infections in humans: the past 100 years.
Parasitology, 2019Co-Authors: Jitender P. Dubey, Sonia AlmeríaAbstract:Cystoisospora belli is a coccidian parasite of humans, with a direct fecal-oral transmission cycle. It is globally distributed, but mainly found in tropical and subtropical areas. Many cases of C. belli infections have been reported in patients with HIV, and in patients undergoing immunosuppressive therapy for organ transplants or those treated for tumours worldwide. Unsporulated or partially sporulated oocysts of C. belli are excreted in feces. When sporulated oocysts in contaminated water or food are ingested, asexual and sexual stages of C. belli are confined to the epithelium of Intestines, bile ducts and gallbladder. Monozoic tissue cysts are present in extra-intestinal organs (lamina propria of the small and large Intestine, Lymph nodes, spleen, and liver) of immunosuppressed humans. However, a paratenic host has not been demonstrated. Cystoisospora belli infections can be persistent, lasting for months, and relapses are common; the mechanism of relapse is unknown. Recently, the endogenous stages of C. belli were re-examined and attention was drawn to cases of misidentification of non-protozoal structures in the gallbladder of patients as C. belli. Here, we review all aspects of the biology of C. belli, including morphology, endogenous stages, prevalence, epidemiology, symptoms, diagnosis and control.
Jitender P. Dubey - One of the best experts on this subject based on the ideXlab platform.
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Cystoisospora belli infections in humans: the past 100 years.
Parasitology, 2019Co-Authors: Jitender P. Dubey, Sonia AlmeríaAbstract:Cystoisospora belli is a coccidian parasite of humans, with a direct fecal-oral transmission cycle. It is globally distributed, but mainly found in tropical and subtropical areas. Many cases of C. belli infections have been reported in patients with HIV, and in patients undergoing immunosuppressive therapy for organ transplants or those treated for tumours worldwide. Unsporulated or partially sporulated oocysts of C. belli are excreted in feces. When sporulated oocysts in contaminated water or food are ingested, asexual and sexual stages of C. belli are confined to the epithelium of Intestines, bile ducts and gallbladder. Monozoic tissue cysts are present in extra-intestinal organs (lamina propria of the small and large Intestine, Lymph nodes, spleen, and liver) of immunosuppressed humans. However, a paratenic host has not been demonstrated. Cystoisospora belli infections can be persistent, lasting for months, and relapses are common; the mechanism of relapse is unknown. Recently, the endogenous stages of C. belli were re-examined and attention was drawn to cases of misidentification of non-protozoal structures in the gallbladder of patients as C. belli. Here, we review all aspects of the biology of C. belli, including morphology, endogenous stages, prevalence, epidemiology, symptoms, diagnosis and control.
Hiroshi Nagura - One of the best experts on this subject based on the ideXlab platform.
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inflammatory bowel disease advances in diagnosis and treatment 3 recent topics 1 biophylaxis observed by Intestine Lymph equipment galt
Nihon Naika Gakkai Zasshi, 1993Co-Authors: Hiroshi NaguraAbstract:腸管リンパ装置(GALT)はパィエル板等リンパ小節を形成するものばかりでなく,腸管の粘膜固有層や上皮間に分布するすべての免疫担当細胞や上皮細胞を含めた腸管全域に及ぶ腸管付属リンパ装置の総称であり, mucosal immune systemの構造的基盤である.それは単に腸内の抗原に反応し,腸管粘膜の保護にあたるのみならず, central Lymphoid organとして免疫担当細胞の分化や全身の免疫能の制御にも関与している.またその免疫機能は,腸管の消化吸収機能と同様に自律神経系によっても支配されており,腸管が“小さい脳”と呼ばれることがある。
Dongmei Fan - One of the best experts on this subject based on the ideXlab platform.
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change of high mobility group box 1 and effect of drainage of Intestine Lymph fluid on rat gut barrier during Intestine ischemia reperfusion
中华临床营养杂志, 2009Co-Authors: Xuefeng Chen, H E Guizhen, Liangguang Dong, Xiaoyu Cui, Hong Shu, Xiurong Wang, Dongmei FanAbstract:Objective To investigate the effects of recombinant human growth hormone(rhGH)on human liver cancer cells expressing different growth hormone receptor(GHR)in vitro.Methods The expressions of GHR on human liver cancer cell lines HepG-2,SMMC7721,and QGY-7701 were detected by immunohistochemical method.Each cell line was assigned to four groups according to the treatment manner:untreated group,50 ng/ml rhGH treated group,100 ng/ml rhGH treated group,and 200 ng/ml rhGH treated group.By methods of methyl thiazol tetrazolium(MTr)assay,5(6)-(N-Succinimidyloxycarbonyl)-3',6'-0,0'-diacetylfluorescein(CFSE)staining,and enzyme-linked immunosorbent assay(ELISA),effects of different concentrations of rhGH on the cell growth,proliferation rate,and excretion of insulin-like growth factor-1(IGF-1)of human liver cancer cell lines were analyzed.The expression of GHR in human liver cancer cell lines pretreated by rhGH was detected by fluorescence and radioligand assays.Results GHR was highly expressed in HepG-2 cells.Compared with the untreated group,the 24-hour growth rates in 50,100,and 200 ng/ml rhGH treated group significantly increased by 107.705%,114.181%,and 107.406%(P＜0.05),and the 48-hour growth rates significantly increased by 109.594%,114.156%,and 109.292%(P＜0.05).The fluorescence intensities of CFSE significantly decreased(P＜0.05).The concentrations of IGF-1 in the four groups were(173.86±27.46)pg/ml,(236.94±19.07)pg/ml,(247.16±14.56)pg/ml,and(217.94±33.61)pg/ml,respecetively;compared with the untreated group,it significantly increased in groups pretreated with different concentrations of rhGH(P＜0.05).By fluorescence,the expressions of GHR significantly increased in the treated groups(P＜0.05).By radioligand assays,the GHR site of the untreated group was(7.51±0.54)×103/cell,with the sites of 50,100,and 200 ng/ml rhGH treated group were(8.44±0.24)x103/cell,(9.40±0.51)×103/cell,and(8.33±0.31)×103/cell (P＜0.05)respectively.However,no obvious effect of rhGH on SMMC7721 was observed,in which the expression of GHR was low;no expression of GHR was detected in QGY-7701(P＞0.05).Conclusions rhGH can promote the growth and increase the expression of GHR in liver cancer cell line HepG-2 in which GHR is highly expressed.However,such effects are not notable in cell line QCY-7701 and SMMC7721 in which the expression of GHR was low or undetectable in vitro. Key words: Recombinant human growth hormone; Growth hormone receptor; Insulin-like growth factor-1; Liver neoplasms
Xuefeng Chen - One of the best experts on this subject based on the ideXlab platform.
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change of high mobility group box 1 and effect of drainage of Intestine Lymph fluid on rat gut barrier during Intestine ischemia reperfusion
中华临床营养杂志, 2009Co-Authors: Xuefeng Chen, H E Guizhen, Liangguang Dong, Xiaoyu Cui, Hong Shu, Xiurong Wang, Dongmei FanAbstract:Objective To investigate the effects of recombinant human growth hormone(rhGH)on human liver cancer cells expressing different growth hormone receptor(GHR)in vitro.Methods The expressions of GHR on human liver cancer cell lines HepG-2,SMMC7721,and QGY-7701 were detected by immunohistochemical method.Each cell line was assigned to four groups according to the treatment manner:untreated group,50 ng/ml rhGH treated group,100 ng/ml rhGH treated group,and 200 ng/ml rhGH treated group.By methods of methyl thiazol tetrazolium(MTr)assay,5(6)-(N-Succinimidyloxycarbonyl)-3',6'-0,0'-diacetylfluorescein(CFSE)staining,and enzyme-linked immunosorbent assay(ELISA),effects of different concentrations of rhGH on the cell growth,proliferation rate,and excretion of insulin-like growth factor-1(IGF-1)of human liver cancer cell lines were analyzed.The expression of GHR in human liver cancer cell lines pretreated by rhGH was detected by fluorescence and radioligand assays.Results GHR was highly expressed in HepG-2 cells.Compared with the untreated group,the 24-hour growth rates in 50,100,and 200 ng/ml rhGH treated group significantly increased by 107.705%,114.181%,and 107.406%(P＜0.05),and the 48-hour growth rates significantly increased by 109.594%,114.156%,and 109.292%(P＜0.05).The fluorescence intensities of CFSE significantly decreased(P＜0.05).The concentrations of IGF-1 in the four groups were(173.86±27.46)pg/ml,(236.94±19.07)pg/ml,(247.16±14.56)pg/ml,and(217.94±33.61)pg/ml,respecetively;compared with the untreated group,it significantly increased in groups pretreated with different concentrations of rhGH(P＜0.05).By fluorescence,the expressions of GHR significantly increased in the treated groups(P＜0.05).By radioligand assays,the GHR site of the untreated group was(7.51±0.54)×103/cell,with the sites of 50,100,and 200 ng/ml rhGH treated group were(8.44±0.24)x103/cell,(9.40±0.51)×103/cell,and(8.33±0.31)×103/cell (P＜0.05)respectively.However,no obvious effect of rhGH on SMMC7721 was observed,in which the expression of GHR was low;no expression of GHR was detected in QGY-7701(P＞0.05).Conclusions rhGH can promote the growth and increase the expression of GHR in liver cancer cell line HepG-2 in which GHR is highly expressed.However,such effects are not notable in cell line QCY-7701 and SMMC7721 in which the expression of GHR was low or undetectable in vitro. Key words: Recombinant human growth hormone; Growth hormone receptor; Insulin-like growth factor-1; Liver neoplasms
