The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
John R Griffiths - One of the best experts on this subject based on the ideXlab platform.
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apc min tumours and normal mouse small Intestines show linear metabolite concentration and dna cytosine hydroxymethylation gradients from pylorus to colon
Scientific Reports, 2020Co-Authors: Basetti Madhu, Santiago Uribelewis, Martin Bachman, Adele Murrell, John R GriffithsAbstract:Topographical variations of metabolite concentrations have been reported in the duodenum, jejunum and ileum of the small intestine, and in human intestinal tumours from those regions, but there are no published metabolite concentrations measurements correlated with linear position in the mouse small intestine or intestinal tumours. Since DNA methylation dynamics are influenced by metabolite concentrations, they too could show linear anatomical variation. We measured metabolites by HR-MAS 1H NMR spectroscopy and DNA cytosine modifications by LC/MS, in normal small Intestines of C57BL/6J wild-type mice, and in normal and tumour samples from ApcMin/+ mice. Wild-type mouse Intestines showed approximately linear, negative concentration gradations from the pylorus (i.e. the junction with the stomach) of alanine, choline compounds, creatine, leucine and valine. ApcMin/+ mouse tumours showed negative choline and valine gradients, but a positive glycine gradient. 5-Hydroxymethylcytosine showed a positive gradient in the tumours. The linear gradients we found along the length of the mouse small intestine and in tumours contrast with previous reports of discrete concentration changes in the duodenum, jejunum and ileum. To our knowledge, this is also the first report of a systematic measurement of global levels of DNA cytosine modification in wild-type and ApcMin/+ mouse small intestine.
Adele Murrell - One of the best experts on this subject based on the ideXlab platform.
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apc min tumours and normal mouse small Intestines show linear metabolite concentration and dna cytosine hydroxymethylation gradients from pylorus to colon
Scientific Reports, 2020Co-Authors: Basetti Madhu, Santiago Uribelewis, Martin Bachman, Adele Murrell, John R GriffithsAbstract:Topographical variations of metabolite concentrations have been reported in the duodenum, jejunum and ileum of the small intestine, and in human intestinal tumours from those regions, but there are no published metabolite concentrations measurements correlated with linear position in the mouse small intestine or intestinal tumours. Since DNA methylation dynamics are influenced by metabolite concentrations, they too could show linear anatomical variation. We measured metabolites by HR-MAS 1H NMR spectroscopy and DNA cytosine modifications by LC/MS, in normal small Intestines of C57BL/6J wild-type mice, and in normal and tumour samples from ApcMin/+ mice. Wild-type mouse Intestines showed approximately linear, negative concentration gradations from the pylorus (i.e. the junction with the stomach) of alanine, choline compounds, creatine, leucine and valine. ApcMin/+ mouse tumours showed negative choline and valine gradients, but a positive glycine gradient. 5-Hydroxymethylcytosine showed a positive gradient in the tumours. The linear gradients we found along the length of the mouse small intestine and in tumours contrast with previous reports of discrete concentration changes in the duodenum, jejunum and ileum. To our knowledge, this is also the first report of a systematic measurement of global levels of DNA cytosine modification in wild-type and ApcMin/+ mouse small intestine.
Christophe Chaillou - One of the best experts on this subject based on the ideXlab platform.
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a layered model of a virtual human intestine for surgery simulation
Medical Image Analysis, 2005Co-Authors: Julien Lenoir, Alexis Angelidis, Philippe Meseure, Mariepaule Cani, Francois Faure, Christophe ChaillouAbstract:In this paper, we propose a new approach to simulate the small intestine in a context of laparoscopic surgery. The ultimate aim of this work is to simulate the training of a basic surgical gesture in real-time: moving aside the intestine to reach hidden areas of the abdomen. The main problem posed by this kind of simulation is animating the intestine. The problem comes from the nature of the intestine: a very long tube which is not isotropically elastic, and is contained in a volume that is small when compared to the intestine’s length. It coils extensively and collides with itself in many places. To do this, we use a layered model to animate the intestine. The intestine’s axis is animated as a linear mechanical component. A specific sphere-based model handles contacts and self-collisions. A skinning model is used to create the intestine’s volume around the axis. This paper discusses and compares three different representations for skinning the intestine: a parametric surface model and two implicit surface models. The first implicit surface model uses point skeletons while the second uses local convolution surfaces. Using these models, we obtained good-looking results in real-time. Some videos of this work can be found at: http://www-imagis.imag.fr/Publications/2004/FLAMCFC04/ .
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A Layered Model of a Virtual Human Intestine for Surgery Simulation
Medical Image Analysis, 2005Co-Authors: Laure France, Julien Lenoir, Alexis Angelidis, Philippe Meseure, Mariepaule Cani, Francois Faure, Christophe ChaillouAbstract:In this paper, we propose a new approach to simulate the small intestine in a context of laparoscopic surgery. The ultimate aim of this work is to simulate the training of a basic surgical gesture in real-time: moving aside the intestine to reach hidden areas of the abdomen. The main problem posed by this kind of simulation is animating the intestine. The problem comes from the nature of the intestine: a very long tube which is not isotropically elastic, and is contained in a volume that is small when compared to the intestine's length. It coils extensively and collides with itself in many places. To do this, we use a layered model to animate the intestine. The intestine's axis is animated as a linear mechanical component. A specific sphere-based model han- dles contacts and self-collisions. A skinning model is used to create the intestine's volume around the axis. This paper discusses and compares three different repre- sentations for skinning the intestine: a parametric surface model and two implicit surface models. The first implicit surface model uses point skeletons while the sec- ond uses local convolution surfaces. Using these models, we obtained good-looking results in real-time.
Basetti Madhu - One of the best experts on this subject based on the ideXlab platform.
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apc min tumours and normal mouse small Intestines show linear metabolite concentration and dna cytosine hydroxymethylation gradients from pylorus to colon
Scientific Reports, 2020Co-Authors: Basetti Madhu, Santiago Uribelewis, Martin Bachman, Adele Murrell, John R GriffithsAbstract:Topographical variations of metabolite concentrations have been reported in the duodenum, jejunum and ileum of the small intestine, and in human intestinal tumours from those regions, but there are no published metabolite concentrations measurements correlated with linear position in the mouse small intestine or intestinal tumours. Since DNA methylation dynamics are influenced by metabolite concentrations, they too could show linear anatomical variation. We measured metabolites by HR-MAS 1H NMR spectroscopy and DNA cytosine modifications by LC/MS, in normal small Intestines of C57BL/6J wild-type mice, and in normal and tumour samples from ApcMin/+ mice. Wild-type mouse Intestines showed approximately linear, negative concentration gradations from the pylorus (i.e. the junction with the stomach) of alanine, choline compounds, creatine, leucine and valine. ApcMin/+ mouse tumours showed negative choline and valine gradients, but a positive glycine gradient. 5-Hydroxymethylcytosine showed a positive gradient in the tumours. The linear gradients we found along the length of the mouse small intestine and in tumours contrast with previous reports of discrete concentration changes in the duodenum, jejunum and ileum. To our knowledge, this is also the first report of a systematic measurement of global levels of DNA cytosine modification in wild-type and ApcMin/+ mouse small intestine.
Andreas Prescher - One of the best experts on this subject based on the ideXlab platform.
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Vascular anatomy of the small intestine—a comparative anatomic study on humans and pigs
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Muhlenbruch, Ulf P Neumann, Uwe Klinge, Volker Schumpelick, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small intestine perfusion. Methods Intestines were obtained from human cadavers ( n = 8; small intestine, n = 51) and from pigs ( n = 10; small intestine, n = 60). Vascularization was visualized with mennige gelatin perfusion and high-resolution mammography. Endothelial cell density was analyzed with immunohistochemistry and factor VIII antibodies. We also investigated the influence of suture techniques (circular anastomoses, n = 19; longitudinal sutures, n = 15) on vascular perfusion. Results Only human samples showed branching of mesenteric vessels. Compared to the pig, human vessels showed closer connections at the entrance to the bowel wall ( p = 0.045) and higher numbers of intramural anastomoses ( p
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Vascular anatomy of the small intestine-a comparative anatomic study on humans and pigs.
International Journal of Colorectal Disease, 2015Co-Authors: Klaus-thilo Von Trotha, Nick Butz, Jochen Grommes, Marcel Binnebösel, Natascha Charalambakis, Georg Muhlenbruch, Ulf P Neumann, Uwe Klinge, Volker Schumpelick, Andreas PrescherAbstract:Background Porcine models are well established for studying intestinal anastomotic healing. In this study, we aimed to clarify the anatomic differences between human and porcine small Intestines. Additionally, we investigated the influences of longitudinal and circular sutures on human small intestine perfusion.
