The Experts below are selected from a list of 16596 Experts worldwide ranked by ideXlab platform
Xuefeng Wang - One of the best experts on this subject based on the ideXlab platform.
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Alpha-synuclein is a potential biomarker in the serum and CSF of patients with Intractable Epilepsy.
Seizure, 2015Co-Authors: Hu Rong, Luo Jin, Wang Wei, Xuefeng WangAbstract:Abstract Purpose Intractable Epilepsy is a brain disorder characterized by recurrent seizures and intracellular alpha-synuclein (αS) deposits; however, the neurobiological basis of this protein accumulation is still poorly understood. This is the first study aiming to assess whether the increase of αS concentrations in the serum and CSF (cerebrospinal fluid) could serve as a marker for αS deposition in the brain and diagnosis of Epilepsy. Methods This investigation enrolled 67 epileptic patients (40 with Intractable Epilepsy; 13 with newly diagnosed Epilepsy, and 14 with non-Intractable Epilepsy). CSF and serum samples were collected from each patient and were assessed by ELISA. Results It was established that the concentration of αS in the CSF and serum was elevated in the Epilepsy patients, as compared to the control. However, the results of the subgroup analysis revealed that levels of αS in the serum and CSF were increased in the Intractable epileptic patients (CSF: 11.12±4.18ng/ml; serum: 52.93±22.11ng/ml), whereas there was no difference in the groups with the newly diagnosed (CSF: 34.998±14.96ng/ml; serum: 7.77±3.41ng/ml) and non-Intractable Epilepsy (CSF: 8.93±4.83ng/ml; serum: 34.11±17.53ng/ml). Conclusion Overall, we found that the rise of the αS content in the serum and CSF may facilitate the identification of Intractable Epilepsy; therefore, the determination of αS rates may serve as a valuable prognostic marker in the clinical assessment.
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Is Intractable Epilepsy a tauopathy
Medical Hypotheses, 2011Co-Authors: Xuefeng Wang, Xiao-fang Shu, Guojun Chen, Fei Xiao, Ji-jun Sun, Xi ZhuAbstract:Abstract Tau exists in neuronal axons and glial cells of the central nervous system and contributes to the maintenance of the unique cell morphology. It functions in axon elongation, cell polarity formation and microtubule stabilization. Aggregates and hyper-phosphorylated tau proteins are classical components of neurofibrillary lesions in numerous neurodegenerative disorders, which are called “tauopathies”. Recent studies have demonstrated that tau-associated genes and proteins and tau phosphorylation were abnormal in Intractable Epilepsy. Therefore, the discovery of the dysfunctional tau in Intractable Epilepsy opens a new window in the study of central tauopathy.
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STUDY ON EXPRESSION OF LAMININ IN PATIENTS WITH Intractable Epilepsy
The International journal of neuroscience, 2009Co-Authors: Yun Feng, Jia-rong Pang, Mei Tang, Xiu-ying Liu, Xuefeng WangAbstract:In this study, we examined differences in serum laminin expression in patients with Intractable Epilepsy. Our results suggest that elevated laminin may contribute to the pathogenesis of Intractable Epilepsy. ELISA and western blots were used to measure laminin in the serum of 30 Intractable Epilepsy patients, 46 nonIntractable Epilepsy patients, and 20 normal subjects. By ELISA, serum laminin levels were greater in Intractable Epilepsy patients (177.396 ± 30.602) and nonIntractable Epilepsy patients (121.915 ± 35.215) than in normal control subjects (67.474 ± 7.197); laminin was significantly greater in the Intractable Epilepsy group than in the nonIntractable Epilepsy group. In western blots, the optical density ratio of laminin to s-actin was 0.871 ± 0.032 for the Intractable Epilepsy group, 0.686 ± 0.017 for the nonIntractable Epilepsy group, and 0.385 ± 0.024 for the normal control group. The optical density ratios of the Intractable and nonIntractable Epilepsy groups were higher than those for the no...
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Nestin in the Temporal Neocortex of the Intractable Epilepsy Patients
Neurochemical Research, 2008Co-Authors: Liang Wang, Zhiqin Xi, Yuqin Li, Xuefeng Wang, Jie Yuan, Zheng XiaoAbstract:Nestin is one kind of intermediate filament protein, which is considered as a typical marker of neural precursor cells. Considerable evidence supports nestin may have actively functions in neurogenesis and gliosis. Our aim was to investigate nestin expression in the temporal neocortex of patients with Intractable Epilepsy (IE), and then to discuss the possible role of nestin in IE. Tissue samples from the temporal neocortex of 32 patients who had surgery for IE were used to detect nestin expression by immunohistochemistry, immunofluorescence. We compared these tissues with 12 histologically normal temporal neocortex from intracranial hypertension patients who had decompression procedures. In this study, we found some nestin positive cells in the normal temporal neocortex, but in the Intractable Epilepsy, they were upregulated, increasing with length of course and seizure frequency. Optical density (OD) value in epileptic tissue was determined 0.246 ± 0.030, and 0.134 ± 0.040 in the control (P < 0.05). Double lables of immunofluorescence showed some nestin positive cells coexpression with glial fibrillary acidic protein (GFAP), while some coexpression with microtubule-associated protein-2 (MAP2). These findings provided some evidence for increased neurogenesis and gliosis in Epilepsy, which could be associated with Intractable Epilepsy.
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Expression of laminin β1 in hippocampi of patients with Intractable Epilepsy
Neuroscience letters, 2008Co-Authors: Xuefeng Wang, Jie Yuan, Hong-bin Sun, Yan Zeng, Tao Lin, Xi ZhuAbstract:We investigated laminin β1 expression in the hippocampi of patients with Intractable Epilepsy and explored the role of laminin β1 in the pathogenesis of this condition. Fluorescence quantitative PCR, immunofluorescence, immunohistochemistry and Western blotting were used to measure laminin β1 expression in surgically removed hippocampi of patients with Intractable Epilepsy, and the results were compared with control hippocampi. Fluorescence quantitative PCR showed increased expression of laminin β1 mRNA in patient hippocampi compared with control tissues. Immunohistochemical staining demonstrated that laminin β1 protein expression was significantly increased in patient hippocampi, and immunofluorescence microscopy showed accumulation of laminin β1 in the cell membrane and cytoplasm of patient hippocampi. These findings were confirmed by Western blotting of protein preparations from patient hippocampi. Elevated expression of laminin β1 mRNA and protein in the hippocampus suggests that laminin β1 may play a role in the development of epileptic seizures in patients with Intractable Epilepsy.
Zheng Xiao - One of the best experts on this subject based on the ideXlab platform.
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Nestin in the Temporal Neocortex of the Intractable Epilepsy Patients
Neurochemical Research, 2008Co-Authors: Liang Wang, Zhiqin Xi, Yuqin Li, Xuefeng Wang, Jie Yuan, Zheng XiaoAbstract:Nestin is one kind of intermediate filament protein, which is considered as a typical marker of neural precursor cells. Considerable evidence supports nestin may have actively functions in neurogenesis and gliosis. Our aim was to investigate nestin expression in the temporal neocortex of patients with Intractable Epilepsy (IE), and then to discuss the possible role of nestin in IE. Tissue samples from the temporal neocortex of 32 patients who had surgery for IE were used to detect nestin expression by immunohistochemistry, immunofluorescence. We compared these tissues with 12 histologically normal temporal neocortex from intracranial hypertension patients who had decompression procedures. In this study, we found some nestin positive cells in the normal temporal neocortex, but in the Intractable Epilepsy, they were upregulated, increasing with length of course and seizure frequency. Optical density (OD) value in epileptic tissue was determined 0.246 ± 0.030, and 0.134 ± 0.040 in the control (P < 0.05). Double lables of immunofluorescence showed some nestin positive cells coexpression with glial fibrillary acidic protein (GFAP), while some coexpression with microtubule-associated protein-2 (MAP2). These findings provided some evidence for increased neurogenesis and gliosis in Epilepsy, which could be associated with Intractable Epilepsy.
T Yoshimoto - One of the best experts on this subject based on the ideXlab platform.
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Surgical treatment of Intractable Epilepsy in children: indication for resective surgery.
Epilepsia, 2000Co-Authors: T Otsuki, T YoshimotoAbstract:Good surgical outcome can be expected for Intractable Epilepsy in children by resective surgery when a focal cortical abnormality is demonstrated by neuroimaging studies such as MRI, SPECT, and PET. Improvement in behavioral and cognitive development can be expected with seizure cessation after surgery. Early surgical intervention for Intractable Epilepsy in children is considered a safe and effective choice of treatment to avoid cognitive delay and learning disability.
Jie Yuan - One of the best experts on this subject based on the ideXlab platform.
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Nestin in the Temporal Neocortex of the Intractable Epilepsy Patients
Neurochemical Research, 2008Co-Authors: Liang Wang, Zhiqin Xi, Yuqin Li, Xuefeng Wang, Jie Yuan, Zheng XiaoAbstract:Nestin is one kind of intermediate filament protein, which is considered as a typical marker of neural precursor cells. Considerable evidence supports nestin may have actively functions in neurogenesis and gliosis. Our aim was to investigate nestin expression in the temporal neocortex of patients with Intractable Epilepsy (IE), and then to discuss the possible role of nestin in IE. Tissue samples from the temporal neocortex of 32 patients who had surgery for IE were used to detect nestin expression by immunohistochemistry, immunofluorescence. We compared these tissues with 12 histologically normal temporal neocortex from intracranial hypertension patients who had decompression procedures. In this study, we found some nestin positive cells in the normal temporal neocortex, but in the Intractable Epilepsy, they were upregulated, increasing with length of course and seizure frequency. Optical density (OD) value in epileptic tissue was determined 0.246 ± 0.030, and 0.134 ± 0.040 in the control (P < 0.05). Double lables of immunofluorescence showed some nestin positive cells coexpression with glial fibrillary acidic protein (GFAP), while some coexpression with microtubule-associated protein-2 (MAP2). These findings provided some evidence for increased neurogenesis and gliosis in Epilepsy, which could be associated with Intractable Epilepsy.
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Expression of laminin β1 in hippocampi of patients with Intractable Epilepsy
Neuroscience letters, 2008Co-Authors: Xuefeng Wang, Jie Yuan, Hong-bin Sun, Yan Zeng, Tao Lin, Xi ZhuAbstract:We investigated laminin β1 expression in the hippocampi of patients with Intractable Epilepsy and explored the role of laminin β1 in the pathogenesis of this condition. Fluorescence quantitative PCR, immunofluorescence, immunohistochemistry and Western blotting were used to measure laminin β1 expression in surgically removed hippocampi of patients with Intractable Epilepsy, and the results were compared with control hippocampi. Fluorescence quantitative PCR showed increased expression of laminin β1 mRNA in patient hippocampi compared with control tissues. Immunohistochemical staining demonstrated that laminin β1 protein expression was significantly increased in patient hippocampi, and immunofluorescence microscopy showed accumulation of laminin β1 in the cell membrane and cytoplasm of patient hippocampi. These findings were confirmed by Western blotting of protein preparations from patient hippocampi. Elevated expression of laminin β1 mRNA and protein in the hippocampus suggests that laminin β1 may play a role in the development of epileptic seizures in patients with Intractable Epilepsy.
Karen Hebert - One of the best experts on this subject based on the ideXlab platform.
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QUESTION 1. Efficacy of vagal nerve stimulation in children with medically Intractable Epilepsy
Archives of Disease in Childhood, 2009Co-Authors: Samir Amin, Karen HebertAbstract:A 12-year-old boy was admitted to our department as part of our Epilepsy surgery protocol. He was admitted for video EEG and for ultimate consideration for vagal nerve stimulator (VNS) insertion. He had a background of Intractable Epilepsy which had not responded to multiple antiepileptic medications. In children with Intractable or refractory or difficult Epilepsy [population], does vagal nerve stimulation [intervention] reduce seizure frequency or rate [outcome]? The search was performed independently by two people. An advanced search of National Library for Health, AMED, BNI, EMBASE, HMIC, MEDLINE, PsycINFO, CINAHL and Health Business Elite was carried out. Key words were: children, Intractable Epilepsy, vagal nerve stimulation, seizure frequency and seizure rate. Fifteen papers were found, six were relevant to the question, and one was excluded due to small size. See table 1. View this table: Table 1 Efficacy of vagal nerve stimulation in children with medically Intractable Epilepsy As approximately one third of patients with Epilepsy will …
