The Experts below are selected from a list of 1605 Experts worldwide ranked by ideXlab platform
Johann Christian Virchow - One of the best experts on this subject based on the ideXlab platform.
-
Against all odds: anti-IgE for Intrinsic Asthma?
Thorax, 2013Co-Authors: Marek Lommatzsch, Stephanie Korn, Roland Buhl, Johann Christian VirchowAbstract:For many years, pathogenetic concepts and the results of clinical trials supported the view that anti-IgE treatment is specifically effective in allergic Asthma. However, there is now growing clinical and mechanistic evidence suggesting that treatment with the anti-IgE antibody omalizumab can be effective in patients with Intrinsic Asthma. Therefore, large and well-controlled clinical trials with anti-IgE are urgently warranted in patients with Intrinsic Asthma. In addition, there is a need to find new biomarkers which can identify patients with Asthma who respond to anti-IgE treatment.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
Pneumologie, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune disease. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy (control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3(+), CD4(+), CD8(+), CD16(+), and CD56(+) subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes as significantly elevated in patients with allergic as well as Intrinsic Asthma when compared with normal control subjects. Analysis of lymphocyte subpopulations also revealed a significant increase in the percentage of CD3(+), CD4(+), CD8(+), and CD56(+) cells expressing perforin in allergic Asthma and a significant increase in the percentage of CD4(+), and CD56(+) cells in Intrinsic Asthma when compare with healthy control subjects. Perforin expression in CD4(+) cells in Intrinsic Asthma was also significantly elevated compared with allergic Asthma. We conclude that allergic and Intrinsic Asthma is associated with increased expression of perforin in T-lymphocyte subsets.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
American Journal of Respiratory and Critical Care Medicine, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma, which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune diseases. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3+, CD4+, CD8+, CD16+, and CD56+ subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes was sign...
E. Ginter - One of the best experts on this subject based on the ideXlab platform.
-
Plasma Zinc, copper and copper/zinc ratio in Intrinsic Asthma
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 1996Co-Authors: Kadrabová J, A. Mad'arić, F. Podivínsky, F. Gazdík, E. GinterAbstract:Summary Plasma zinc and copper levels and copper/zinc ratio of 22 Intrinsic Asthma patients were compared to that of 33 healthy control subjects. Five of the Intrinsic Asthma patients were aspirin (ASA) intolerant. The zinc content of plasma was found to be significantly lower in patients than in control individuals with the values being 0.80 ± 0.01 mg/L versus 0.89 ± 0.02 mg/L, while the plasma copper level and copper/zinc ratio were significantly higher in the Asthma group than in the control group, with the values bieng 1.28 ± 0.03 mg/L and 1.61 ± 0.04 versus 1.06 ± 0.02 mg/L and 1.21 ± 0.02, respectively (mean ± SE). The role of the essential trace elements zinc and copper and cytokines in the pathogenesis of Asthma is discussed.
-
plasma zinc copper and copper zinc ratio in Intrinsic Asthma
Journal of Trace Elements in Medicine and Biology, 1996Co-Authors: J Kadrabova, F. Podivínsky, F. Gazdík, A Madaric, E. GinterAbstract:Summary Plasma zinc and copper levels and copper/zinc ratio of 22 Intrinsic Asthma patients were compared to that of 33 healthy control subjects. Five of the Intrinsic Asthma patients were aspirin (ASA) intolerant. The zinc content of plasma was found to be significantly lower in patients than in control individuals with the values being 0.80 ± 0.01 mg/L versus 0.89 ± 0.02 mg/L, while the plasma copper level and copper/zinc ratio were significantly higher in the Asthma group than in the control group, with the values bieng 1.28 ± 0.03 mg/L and 1.61 ± 0.04 versus 1.06 ± 0.02 mg/L and 1.21 ± 0.02, respectively (mean ± SE). The role of the essential trace elements zinc and copper and cytokines in the pathogenesis of Asthma is discussed.
H Matthys - One of the best experts on this subject based on the ideXlab platform.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
Pneumologie, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune disease. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy (control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3(+), CD4(+), CD8(+), CD16(+), and CD56(+) subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes as significantly elevated in patients with allergic as well as Intrinsic Asthma when compared with normal control subjects. Analysis of lymphocyte subpopulations also revealed a significant increase in the percentage of CD3(+), CD4(+), CD8(+), and CD56(+) cells expressing perforin in allergic Asthma and a significant increase in the percentage of CD4(+), and CD56(+) cells in Intrinsic Asthma when compare with healthy control subjects. Perforin expression in CD4(+) cells in Intrinsic Asthma was also significantly elevated compared with allergic Asthma. We conclude that allergic and Intrinsic Asthma is associated with increased expression of perforin in T-lymphocyte subsets.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
American Journal of Respiratory and Critical Care Medicine, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma, which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune diseases. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3+, CD4+, CD8+, CD16+, and CD56+ subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes was sign...
-
Inflammatory determinants of Asthma severity: mediator and cellular changes in bronchoalveolar lavage fluid of patients with severe Asthma.
The Journal of allergy and clinical immunology, 1996Co-Authors: J.c. Virchow, C. Walker, Claus Kroegel, H MatthysAbstract:Cellular and mediator profiles in bronchoalveolar lavage have not been compared systematically between patients with Asthma of different severities, mainly because the patients with more severe Asthma have an increased need for antiinflammatory medication. Information is limited to comparisons of allergic and Intrinsic Asthma, which can be distinguished clinically. When patients from these two groups with similar degrees of bronchial hyperresponsiveness were compared, both groups showed increased numbers of activated T-helper lymphocytes; those in the allergic group expressed the IL-2 receptor (CD25+), whereas in patients with Intrinsic Asthma there was also an increased number of T-suppressor cells with the activation markers CD25, class II histocompatibility antigen, and very late activation antigen-I, as well as T-helper cells class II histocompatibility antigen and very late activation antigen-I. This pattern is compatible with a more chronic T-cell activation in patients with Intrinsic Asthma. In patients with allergic Asthma the cytokine pattern is compatible with a pure TH2 response (elevated IL-4 and IL-5); however, Intrinsic Asthma is characterized by elevated IL-5 and IL-2 but not IL-4. Our own findings show similar concentrations of IL-1, IL-8, and granulocyte-macrophage colony-stimulating factor in bronchoalveolar lavage fluid of patients with allergic and Intrinsic Asthma, whereas IL-6 and interferon-gamma tended to be higher in patients with Intrinsic Asthma. There are probably fundamental differences in the pathogenesis of allergic and Intrinsic Asthma. These findings suggest that Asthma does not depend on the presence of IgE or IL-4, although both may contribute to the pathogenesis of atopic Asthma. The only common pathway in the different presentations of Asthma that has been related to clinical symptoms appears to be IL-5-mediated activation of eosinophils; therapies aimed at this mechanism may be promising.
-
Immunological differences in allergic and Intrinsic Asthma
Asthma, 1993Co-Authors: C. Kroegel, H MatthysAbstract:Intrinsic Asthmatics have a different pattern of T cell activation which can be observed in periphal blood but more pronouced in BAL. In contrast to allergic Asthmatics CD23+ B cells are not elevated in the periphal circulation of Intrinsic Asthmatics.
J Kadrabova - One of the best experts on this subject based on the ideXlab platform.
-
plasma zinc copper and copper zinc ratio in Intrinsic Asthma
Journal of Trace Elements in Medicine and Biology, 1996Co-Authors: J Kadrabova, F. Podivínsky, F. Gazdík, A Madaric, E. GinterAbstract:Summary Plasma zinc and copper levels and copper/zinc ratio of 22 Intrinsic Asthma patients were compared to that of 33 healthy control subjects. Five of the Intrinsic Asthma patients were aspirin (ASA) intolerant. The zinc content of plasma was found to be significantly lower in patients than in control individuals with the values being 0.80 ± 0.01 mg/L versus 0.89 ± 0.02 mg/L, while the plasma copper level and copper/zinc ratio were significantly higher in the Asthma group than in the control group, with the values bieng 1.28 ± 0.03 mg/L and 1.61 ± 0.04 versus 1.06 ± 0.02 mg/L and 1.21 ± 0.02, respectively (mean ± SE). The role of the essential trace elements zinc and copper and cytokines in the pathogenesis of Asthma is discussed.
Verena Arnold - One of the best experts on this subject based on the ideXlab platform.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
Pneumologie, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune disease. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy (control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3(+), CD4(+), CD8(+), CD16(+), and CD56(+) subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes as significantly elevated in patients with allergic as well as Intrinsic Asthma when compared with normal control subjects. Analysis of lymphocyte subpopulations also revealed a significant increase in the percentage of CD3(+), CD4(+), CD8(+), and CD56(+) cells expressing perforin in allergic Asthma and a significant increase in the percentage of CD4(+), and CD56(+) cells in Intrinsic Asthma when compare with healthy control subjects. Perforin expression in CD4(+) cells in Intrinsic Asthma was also significantly elevated compared with allergic Asthma. We conclude that allergic and Intrinsic Asthma is associated with increased expression of perforin in T-lymphocyte subsets.
-
increase in perforin positive peripheral blood lymphocytes in extrinsic and Intrinsic Asthma
American Journal of Respiratory and Critical Care Medicine, 2000Co-Authors: Verena Arnold, S Balkow, R Staats, H Matthys, Werner Luttmann, Johann Christian VirchowAbstract:The cause of Asthma, which has been linked to a chronic, T-cell-mediated bronchial inflammation, remains unclear. A number of other T-lymphocyte-mediated, chronic inflammatory disorders have been associated with autoimmunity and there are data indicating that autoimmune phenomena might also be present in Asthma. Expression of perforin, a cytotoxic molecule produced by lymphocytes, has been implicated in the pathogenesis of autoimmune diseases. We therefore tested the hypothesis that allergic and Intrinsic Asthma might be associated with an increase in lymphocytes producing perforin by comparing the expression of intracellular perforin in peripheral blood lymphocytes of patients with extrinsic Asthma (n = 13), Intrinsic Asthma (n = 7), and healthy control subjects (n = 18). Lymphocytes were identified using flow cytometry and subdivided into CD3+, CD4+, CD8+, CD16+, and CD56+ subpopulations after staining with appropriate monoclonal antibodies. The percentage of perforin-positive total lymphocytes was sign...
