The Experts below are selected from a list of 36066 Experts worldwide ranked by ideXlab platform
Heinrich Hofmann - One of the best experts on this subject based on the ideXlab platform.
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significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle spion based core shell Nanoparticles on the composition of the protein corona
Biomaterials Science, 2015Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Margarethe Hofmannamtenbrink, Marcel De Vries, M M Motazacker, Farhad Rezaee, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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Significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle (SPION) based core/shell Nanoparticles on the composition of the protein corona
Biomaterials science, 2014Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Marcel De Vries, M M Motazacker, Farhad Rezaee, Margarethe Hofmann-amtenbrink, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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intraarticular application of superparamagnetic Nanoparticles and their uptake by synovial membrane an experimental study in sheep
Journal of Magnetism and Magnetic Materials, 2005Co-Authors: Katja Schulze, Heinrich Hofmann, Annette Koch, Bernhard Schopf, A Petri, Benedikt Steitz, Mathieu Chastellain, M Hofmann, Brigitte Von RechenbergAbstract:A superparamagnetic Iron Oxide Nanoparticle, coated with polyvinyl alcohol, (PVA-SPION) and its fluorescently functionalized analogue (amino-PVA-Cy3.5-SPION) were compared in vivo as proof of principle for future use in magnetic drug targeting in inflammatory joint diseases. They were injected either intraarticularly or periarticularly and their uptake by cells of the synovial membrane was evaluated. Uptake was completed in 48 h and was enforced by an extracorporally applied magnet.
Miqin Zhang - One of the best experts on this subject based on the ideXlab platform.
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superparamagnetic Iron Oxide Nanoparticle based delivery systems for biotherapeutics
Expert Opinion on Drug Delivery, 2013Co-Authors: Hyejung Mok, Miqin ZhangAbstract:Introduction: Superparamagnetic Iron Oxide Nanoparticle (SPION)-based carrier systems have many advantages over other Nanoparticle-based systems. They are biocompatible, biodegradable, facilely tunable and superparamagnetic and thus controllable by an external magnetic field. These attributes enable their broad biomedical applications. In particular, magnetically driven carriers are drawing considerable interest as an emerging therapeutic delivery system because of their superior delivery efficiency. Areas covered: This article reviews the recent advances in use of SPION-based carrier systems to improve the delivery efficiency and target specificity of biotherapeutics. The authors examine various formulations of SPION-based delivery systems, including SPION micelles, clusters, hydrogels, liposomes and micro/nanospheres, as well as their specific applications in delivery of biotherapeutics. Expert opinion: Recently, biotherapeutics including therapeutic cells, proteins and genes have been studied as altern...
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doxorubicin loaded Iron Oxide Nanoparticles overcome multidrug resistance in cancer in vitro
Journal of Controlled Release, 2011Co-Authors: Forrest M Kievit, Hyejung Mok, Freddy Y Wang, Chen Fang, Kui Wang, John R Silber, Richard G Ellenbogen, Miqin ZhangAbstract:Multidrug resistance (MDR) is characterized by the overexpression of ATP-binding cassette (ABC) transporters that actively pump a broad class of hydrophobic chemotherapeutic drugs out of cancer cells. MDR is a major mechanism of treatment resistance in a variety of human tumors, and clinically applicable strategies to circumvent MDR remain to be characterized. Here we describe the fabrication and characterization of a drug-loaded Iron Oxide Nanoparticle designed to circumvent MDR. Doxorubicin (DOX), an anthracycline antibiotic commonly used in cancer chemotherapy and substrate for ABC-mediated drug efflux, was covalently bound to polyethylenimine via a pH sensitive hydrazone linkage and conjugated to an Iron Oxide Nanoparticle coated with amine terminated polyethylene glycol. Drug loading, physiochemical properties and pH lability of the DOX-hydrazone linkage were evaluated in vitro. Nanoparticle uptake, retention, and dose-dependent effects on viability were compared in wild-type and DOX-resistant ABC transporter over-expressing rat glioma C6 cells. We found that DOX release from Nanoparticles was greatest at acidic pH, indicative of cleavage of the hydrazone linkage. DOX-conjugated Nanoparticles were readily taken up by wild-type and drug-resistant cells. In contrast to free drug, DOX-conjugated Nanoparticles persisted in drug-resistant cells, indicating that they were not subject to drug efflux. Greater retention of DOX-conjugated Nanoparticles was accompanied by reduction of viability relative to cells treated with free drug. Our results suggest that DOX-conjugated Nanoparticles could improve the efficacy of chemotherapy by circumventing MDR.
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Surface engineering of Iron Oxide Nanoparticles for targeted cancer therapy
Accounts of Chemical Research, 2011Co-Authors: Forrest M Kievit, Miqin ZhangAbstract:Nanotechnology provides a flexible platform for the development of effective therapeutic nanomaterials that can interact specifically with a target in a biological system and provoke a desired response. Of the nanomaterials studied, Iron Oxide Nanoparticles have emerged as one of top candidates for cancer therapy. Their intrinsic superparamagnetism enables noninvasive magnetic resonance imaging (MRI), and their biodegradability is advantageous for in vivo applications. A therapeutic superparamagnetic Iron Oxide Nanoparticle (SPION) typically consists of three primary components: an Iron Oxide Nanoparticle core that serves as both a carrier for therapeutics and contrast agent for MRI, a coating on the Iron Oxide Nanoparticle that promotes favorable interactions between the SPION and the biological system, and a therapeutic payload that performs the designated function in vivo. Often, the design may include a targeting ligand that recognizes the receptors over-expressed on the exterior surface of cancer cel...
Morteza Mahmoudi - One of the best experts on this subject based on the ideXlab platform.
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significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle spion based core shell Nanoparticles on the composition of the protein corona
Biomaterials Science, 2015Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Margarethe Hofmannamtenbrink, Marcel De Vries, M M Motazacker, Farhad Rezaee, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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Significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle (SPION) based core/shell Nanoparticles on the composition of the protein corona
Biomaterials science, 2014Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Marcel De Vries, M M Motazacker, Farhad Rezaee, Margarethe Hofmann-amtenbrink, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
Usawadee Sakulkhu - One of the best experts on this subject based on the ideXlab platform.
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significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle spion based core shell Nanoparticles on the composition of the protein corona
Biomaterials Science, 2015Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Margarethe Hofmannamtenbrink, Marcel De Vries, M M Motazacker, Farhad Rezaee, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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Significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle (SPION) based core/shell Nanoparticles on the composition of the protein corona
Biomaterials science, 2014Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Marcel De Vries, M M Motazacker, Farhad Rezaee, Margarethe Hofmann-amtenbrink, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
Lionel Maurizi - One of the best experts on this subject based on the ideXlab platform.
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significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle spion based core shell Nanoparticles on the composition of the protein corona
Biomaterials Science, 2015Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Margarethe Hofmannamtenbrink, Marcel De Vries, M M Motazacker, Farhad Rezaee, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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Significance of surface charge and shell material of superparamagnetic Iron Oxide Nanoparticle (SPION) based core/shell Nanoparticles on the composition of the protein corona
Biomaterials science, 2014Co-Authors: Usawadee Sakulkhu, Morteza Mahmoudi, Lionel Maurizi, Geraldine Coullerez, Marcel De Vries, M M Motazacker, Farhad Rezaee, Margarethe Hofmann-amtenbrink, Heinrich HofmannAbstract:As Nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" Nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium diOxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : Nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the Nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.
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Monitoring the effects of dexamethasone treatment by MRI using in vivo Iron Oxide Nanoparticle-labeled macrophages
Arthritis Research and Therapy, 2014Co-Authors: Azza Gramoun, Lionel Maurizi, Geraldine Coullerez, Lindsey Crowe, Wolfgang Wirth, Frank Tobalem, Kerstin Grosdemange, Felix Eckstein, Marije Koenders, Wim Van Den BergAbstract:Introduction: Rheumatoid arthritis (RA) is a chronic disease causing recurring inflammatory joint attacks. These attacks are characterized by macrophage infiltration contributing to joint destruction. Studies have shown that RA treatment efficacy is correlated to synovial macrophage number. The aim of this study was to experimentally validate the use of in vivo superparamagnetic Iron Oxide Nanoparticle (SPION) labeled macrophages to evaluate RA treatment by MRI. Methods: The evolution of macrophages was monitored with and without dexamethasone (Dexa) treatment in rats. Two doses of 3 and 1 mg/kg Dexa were administered two and five days following induction of antigen induced arthritis. SPIONs (7 mg Fe/rat) were injected intravenously and the knees were imaged in vivo on days 6, 10 and 13. The MR images were scored for three parameters: SPION signal intensity, SPION distribution pattern and synovial oedema. Using 3D semi-automated software, the MR SPION signal was quantified. The efficacy of SPIONs and gadolinium chelate (Gd), an MR contrast agent, in illustrating treatment effects were compared. Those results were confirmed through histological measurements of number and area of macrophages and Nanoparticle clusters using CD68 immunostaining and Prussian blue staining respectively.
