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Tsutomu Takeuchi - One of the best experts on this subject based on the ideXlab platform.
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Entamoeba dispar: cultivation with sterilized Crithidia fasciculata.
The Journal of eukaryotic microbiology, 1998Co-Authors: Seiki Kobayashi, Hiroshi Tachibana, Eiko Imai, Tatsushi Fujiwara, Tsutomu TakeuchiAbstract:Four isolates of Entamoeba dispar identified by their hexokinase and phosphoglucomutase Isoenzyme profile and by their failure to react with Entamoeba histolytica-specific monoclonal antibody (4G6) could be grown in either Diamond's BI-S-33 medium, newly developed BCSI-S (Biosate cysteine starch iron-serum) medium, or casein-free YI-S medium in the presence of Crithidia fasciculata (ReF-1:PRR) sterilized by heating 56 degrees C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4 degrees C. After the cultures were maintained for over 50 passages, the amebae were identified as E. dispar by Isoenzyme Analysis, polymerase chain reaction with E. histolytica- and E. dispar-specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic Analysis. All of these findings suggest that E. dispar can grow in vitro with metabolically inactive C. fasciculata as a culture associate.
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Entamoeba dispar : Cultivation with sterilized Crithidia fasciculata : Host-parasite interactions in amebiasis
Journal of Eukaryotic Microbiology, 1998Co-Authors: Seiki Kobayashi, Hiroshi Tachibana, Eiko Imai, Tatsushi Fujiwara, Tsutomu TakeuchiAbstract:Four isolates of Entamoeba dispar identified by their hexokinase and phosphoglucomutase Isoenzyme profile and by their failure to react with Entamoeba histolytica-specific monoclonal antibody (4G6) could be grown in either Diamond's BI-S-33 medium, newly developed BCSI-S (Biosate cysteine starch iron-serum) medium, or casein-free YI-S medium in the presence of Crithidia fasciculata (ReF-1:PRR) sterilized by heating 56° C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4° C. After the cultures were maintained for over 50 passages, the amebae were identified as E. dispar by Isoenzyme Analysis, polymerase chain reaction with E. histolytica- and E. dispar-specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic Analysis. All of these findings suggest that E. dispar can grow in vitro with metabolically inactive C. fasciculata as a culture associate.
Jeanpierre Dedet - One of the best experts on this subject based on the ideXlab platform.
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geographical distribution and epidemiological features of old world leishmania infantum and leishmania donovani foci based on the Isoenzyme Analysis of 2277 strains
Parasitology, 2013Co-Authors: F Pratlong, Christophe Ravel, J Dereure, Patrick Lami, Yves Balard, Ghislaine Serres, Fouad El Baidouri, Jeanpierre DedetAbstract:A series of 2277 Leishmania strains from Old World visceral leishmaniasis foci, isolated between 1973 and 2008, were studied by Isoenzyme Analysis. The strains were obtained from humans, domestic and wild carnivores, rodents and phlebotomine sandflies, and came from 36 countries. In all, 60 different zymodemes were identified and clustered by a phenetic Analysis into 3 different groups corresponding to the typically visceralizing species L. donovani (20 zymodemes, 169 strains), L. archibaldi (3 zymodemes, 46 strains) and L. infantum (37 zymodemes, 2,062 strains). The taxonomic position of these isoenzymatic groups is discussed in view of contradictory results obtained from recent molecular studies.
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geographical distribution and epidemiological features of old world cutaneous leishmaniasis foci based on the Isoenzyme Analysis of 1048 strains
Tropical Medicine & International Health, 2009Co-Authors: F Pratlong, Christophe Ravel, J Dereure, Patrick Lami, Yves Balard, Ghislaine Serres, Genevieve Lanotte, J A Rioux, Jeanpierre DedetAbstract:A series of 1048 Leishmania strains from Old World cutaneous leishmaniasis foci, isolated between 1981 and 2005, were studied by Isoenzyme Analysis. The strains were obtained from humans, rodents, dogs and sandflies from 33 countries. The four typically dermotropic species, Leishmania major, L. tropica, L. aethiopica and L. killicki, were found. The viscerotropic species L. donovani and L. infantum, which can occasionally be responsible for cutaneous leishmaniasis, are not considered in this paper. Leishmania major was the least polymorphic species (12 zymodemes, 638 strains). Leishmania tropica was characterized by a complex polymorphism varying according to focus (35 zymodemes, 329 strains). Leishmania aethiopica, a species restricted to East Africa, showed a high polymorphism, in spite of a limited number of strains (23 zymodemes, 40 strains). Leishmania killicki, mainly restricted to Tunisia had a single zymodeme for 39 strains. Recently a parasite close to L. killicki (one zymodeme, two strains) was isolated in Algeria, which lead us to revise the taxonomic status of this taxon.
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a previously unclassified trypanosomatid responsible for human cutaneous lesions in martinique french west indies is the most divergent member of the genus leishmania ss
Parasitology, 2002Co-Authors: Harry Noyes, F Pratlong, M L Chance, John Ellis, G Lanotte, Jeanpierre DedetAbstract:Two cases of skin lesions similar to those caused by Leishmania parasites have been reported from Martinique. Parasites isolated from these lesions were unlike Leishmania reference strains by Isoenzyme Analysis and electron microscopy and were assumed to be monoxenous trypanosomatids which normally only infect invertebrates. Both strains have now been retyped by Isoenzyme Analysis and found to be identical to each other and distantly related to all other Leishmania species. The sequence of the 18S ribosomal RNA gene and partial sequences of the DNA polymerase alpha and RNA polymerase II largest subunit genes were obtained. These sequences indicated that the Martinique parasites clustered with L. enriettii and were basal to all other euleishmania. However, support for both the position basal to all euleishmania and the clustering with L. enriettii was low. The Martinique parasites may cluster with L. (Leishmania) or L. (Viannia) or form a novel clade within the euleishmania either with or without L. enriettii.
Saliha Mi Haji - One of the best experts on this subject based on the ideXlab platform.
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retrospective evaluation of a local protocol used to enhance laboratory savings through minimizing the performance of alkaline phosphatase Isoenzyme Analysis
Annals of Clinical Biochemistry, 2019Co-Authors: Saliha Mi Haji, Allison Chipchase, William D Fraser, Javier Romero GomezAbstract:BackgroundAlkaline phosphatase Isoenzyme Analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally derived screening algorithm for alkaline phosphatase isoen...
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Retrospective evaluation of a local protocol used to enhance laboratory savings through minimizing the performance of alkaline phosphatase Isoenzyme Analysis
'SAGE Publications', 2019Co-Authors: Saliha Mi Haji, Chipchase Allison, Fraser, William D, Gomez JavierAbstract:Background: Alkaline phosphatase Isoenzyme Analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally derived screening algorithm for alkaline phosphatase Isoenzyme requests on the number of alkaline phosphatase Isoenzyme analyses performed, laboratory cost and patient care. Method: A total of 110 alkaline phosphatase Isoenzyme Analysis requests from the year 2015 were reviewed and subsequent alkaline phosphatase concentrations were monitored over a two-year period, to determine if the decision of performing/not performing alkaline phosphatase Isoenzyme Analysis, based on the algorithm, had an impact on patient care and laboratory cost. All alkaline phosphatase Isoenzyme Analysis requests with two consecutive elevated alkaline phosphatase concentrations (>upper limit of reference interval) were screened and, subject to the gamma glutamyl transferase being within the reference interval, either Bone alkaline phosphatase or 25 hydroxyvitamin D was measured depending on the age of the patient. Results: Compliance with this algorithm led to the normalization of subsequent serum alkaline phosphatase in 97% of patients without requiring alkaline phosphatase Isoenzyme Analysis. The cost of performing Bone alkaline phosphatase and 25 hydroxyvitamin D in-house was £778.50, while the cost of performing alkaline phosphatase Isoenzyme Analysis would have been £3040. This resulted in a laboratory saving of £2261.50. Conclusions: Implementation of this algorithm led to a significant reduction in alkaline phosphatase Isoenzyme Analysis, without compromising patient care. Total savings could be increased if 25 hydroxyvitamin D was used as a first-line test, for all patients with an elevated alkaline phosphatase and a normal gamma glutamyl transferase regardless of age. This algorithm is cost-effective and can be implemented in laboratories with 25 hydroxyvitamin D assay
Raphael Breuer - One of the best experts on this subject based on the ideXlab platform.
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lactate dehydrogenase Isoenzyme Analysis for the diagnosis of pleural effusion in haemato oncological patients
Respiratory Medicine, 1999Co-Authors: Izidore S Lossos, Orna Intrator, Neville Berkman, Raphael BreuerAbstract:This study aimed to evaluate the utility of the pleural fluid lactate dehydrogenase (LDH) Isoenzyme algorithm for the differential diagnosis of pleural fluid in patients with haematological malignancies. Twenty consecutive haemato-oncological patients with pleural effusion, hospitalized in the Haematology Department during a 2.75-year period, were prospectively and independently evaluated for the cause of effusion by standard methods and the LDH Isoenzyme algorithm. The causes of the pleural effusions established during the standard evaluations were compared to the results obtained from the LDH Isoenzyme algorithm. Following the standard evaluation, the pleural effusion was attributed to congestive heart failure in one patient, to infection in six, to the underlying malignancy in 12 and to concomitant congestive heart failure and malignancy in one. LDH Isoenzyme Analysis correctly predicted the cause of pleural effusion in 18 patients (positive predictive value 90%). In haemato-oncological patients, the pleural fluid LDH Isoenzyme pattern may be helpful in the differential diagnosis of the most common causes of pleural effusion.
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differential diagnosis of pleural effusion by lactate dehydrogenase Isoenzyme Analysis
Chest, 1997Co-Authors: Izidore S Lossos, Raphael Breuer, Orna Intrator, Moshe SonenblickAbstract:Study objective To determine the diagnostic value of pleural fluid lactate dehydrogenase (LDH) Isoenzyme Analysis in the differential diagnosis of pleural fluid. Patients and methods Eighty-seven consecutive patients with pleural effusion caused by congestive heart failure (33), infection (33), and malignancy (21) comprised a derivation set of patients. Pleural fluid LDH activity and Isoenzyme pattern were established in all patients and analyzed by the classification and regression trees (CART) method. An additional group of 20 consecutive patients comprised a validation set that was used for cross-validation of CART-derived decision tree. Results A decision tree, with a positive predictive value of 83%, was constructed and validated by data from a validation set of patients. Conclusions Pleural fluid LDH Isoenzyme pattern may be helpful for the differential diagnosis of the most common causes of pleural effusions: congestive heart failure, infections, and malignancy.
Javier Romero Gomez - One of the best experts on this subject based on the ideXlab platform.
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retrospective evaluation of a local protocol used to enhance laboratory savings through minimizing the performance of alkaline phosphatase Isoenzyme Analysis
Annals of Clinical Biochemistry, 2019Co-Authors: Saliha Mi Haji, Allison Chipchase, William D Fraser, Javier Romero GomezAbstract:BackgroundAlkaline phosphatase Isoenzyme Analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally derived screening algorithm for alkaline phosphatase isoen...
