The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
De Chen - One of the best experts on this subject based on the ideXlab platform.
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Islet Isolation and Purification from inbred Wuzhishan miniature pigs.
Xenotransplantation, 2012Co-Authors: Xiaofeng Jiang, Qian Taolai, Thomas Linn, Liangqi Cao, Guangyang Xiang, Yezeng Wang, He-ping Peng, Ping Xue, Le-hong Zhang, De ChenAbstract:Jiang X, Qian T, Linn T, Cao L, Xiang G, Wang Y, Peng H, Xue P, Zhang L, Chen D, Yang X. Islet Isolation and Purification from inbred Wuzhishan miniature pigs. Xenotransplantation 2012; 19: 159–165. © 2012 John Wiley & Sons A/S. Abstract: Background: To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet Isolation and Purification. Methods: Islet Isolation and Purification was conducted on adult (1-yr-old), male inbred WZS miniature pigs and age- and sex-matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet Isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose-induced insulin release in culture and transplantation in an in vivo study. Results: The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs’ pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents [IEQ]/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2–4 days (median, 3.6 days) in experimental group and control group, respectively. Conclusion: Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
Yu Hai-dong - One of the best experts on this subject based on the ideXlab platform.
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Pancreatic Islet Isolation and Purification of Yunnan Miniature Pig
Journal of Kunming Medical University, 2009Co-Authors: Yu Hai-dongAbstract:Objective To explore an effective way of pancreatic islet cell Isolation and Purification of Yunnan Miniature Pig.Methods Islets were isolated using digestion by pancreatic duct injection of collagenase,and still digestion combined with the physical digestion.Islet cells were purified by the proportion of discontinuous density gradient centrifugation;it's function was detected by insulin release test.Results(4 580±257)IEQ per gram of pancreas were obtained,after Purification the recover were(3 660±449)IEQ per gram of pancreas;the purify of the final preparation was about 80%.The release of insulin was well received.Conclusions The production and purity of island cells were high and well-functioning by this method.It provides experimental evidence for the Isolation and Purification of adult islet cells.
Xiaofeng Jiang - One of the best experts on this subject based on the ideXlab platform.
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Islet Isolation and Purification from inbred Wuzhishan miniature pigs.
Xenotransplantation, 2012Co-Authors: Xiaofeng Jiang, Qian Taolai, Thomas Linn, Liangqi Cao, Guangyang Xiang, Yezeng Wang, He-ping Peng, Ping Xue, Le-hong Zhang, De ChenAbstract:Jiang X, Qian T, Linn T, Cao L, Xiang G, Wang Y, Peng H, Xue P, Zhang L, Chen D, Yang X. Islet Isolation and Purification from inbred Wuzhishan miniature pigs. Xenotransplantation 2012; 19: 159–165. © 2012 John Wiley & Sons A/S. Abstract: Background: To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet Isolation and Purification. Methods: Islet Isolation and Purification was conducted on adult (1-yr-old), male inbred WZS miniature pigs and age- and sex-matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet Isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose-induced insulin release in culture and transplantation in an in vivo study. Results: The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs’ pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents [IEQ]/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2–4 days (median, 3.6 days) in experimental group and control group, respectively. Conclusion: Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
Lei Shi - One of the best experts on this subject based on the ideXlab platform.
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Bioactivities, Isolation and Purification methods of polysaccharides from natural products: A review
International journal of biological macromolecules, 2016Co-Authors: Lei ShiAbstract:Polysaccharides play multiple roles and have extensive bioactivities in life process and an immense potential in healthcare, food and cosmetic industries, due to their therapeutic effects and relatively low toxicity. This review describes their major functions involved in antitumor, anti-virus, and anti-inflammatory bioactivities. Due to their enormous structural heterogeneity, the approaches for Isolation and Purification of polysaccharides are distinct from that of the other macromolecules such as proteins, etc. Yet, to achieve the homogeneity is the initial step for studies of polysaccharide structure, pharmacology, and its structure-activity relationships. According to the experiences accumulated by our lab and the published literatures, this review also introduces the methods widely used in Isolation and Purification of polysaccharides.
Ping Xue - One of the best experts on this subject based on the ideXlab platform.
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Islet Isolation and Purification from inbred Wuzhishan miniature pigs.
Xenotransplantation, 2012Co-Authors: Xiaofeng Jiang, Qian Taolai, Thomas Linn, Liangqi Cao, Guangyang Xiang, Yezeng Wang, He-ping Peng, Ping Xue, Le-hong Zhang, De ChenAbstract:Jiang X, Qian T, Linn T, Cao L, Xiang G, Wang Y, Peng H, Xue P, Zhang L, Chen D, Yang X. Islet Isolation and Purification from inbred Wuzhishan miniature pigs. Xenotransplantation 2012; 19: 159–165. © 2012 John Wiley & Sons A/S. Abstract: Background: To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet Isolation and Purification. Methods: Islet Isolation and Purification was conducted on adult (1-yr-old), male inbred WZS miniature pigs and age- and sex-matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet Isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose-induced insulin release in culture and transplantation in an in vivo study. Results: The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs’ pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents [IEQ]/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2–4 days (median, 3.6 days) in experimental group and control group, respectively. Conclusion: Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
