The Experts below are selected from a list of 66030 Experts worldwide ranked by ideXlab platform
Pieter Vader - One of the best experts on this subject based on the ideXlab platform.
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extracellular vesicle heterogeneity subpopulations Isolation Techniques and diverse functions in cancer progression
Frontiers in Immunology, 2018Co-Authors: Eduard Willms, Carlos Cabanas, Imre Mager, Matthew J A Wood, Pieter VaderAbstract:Cells release membrane enclosed nano-sized vesicles termed extracellular vesicles (EVs) that function as mediators of intercellular communication by transferring biological information between cells. Tumor-derived EVs have emerged as important mediators in cancer development and progression, mainly through transfer of their bioactive content which can include oncoproteins, oncogenes, chemokine receptors, as well as soluble factors, transcripts of proteins and miRNAs involved in angiogenesis or inflammation. This transfer has been shown to influence the metastatic behavior of primary tumors. Moreover, tumor-derived EVs have been shown to influence distant cellular niches, establishing favorable microenvironments that support growth of disseminated cancer cells upon their arrival at these pre-metastatic niches. It is generally accepted that cells release a number of major EV populations with distinct biophysical properties and biological functions. Exosomes, microvesicles, and apoptotic bodies are EV populations most widely studied and characterized. They are discriminated based primarily on their intracellular origin. However, increasing evidence suggests that even within these EV populations various subpopulations may exist. This heterogeneity introduces an extra level of complexity in the study of EV biology and function. For example, EV subpopulations could have unique roles in the intricate biological processes underlying cancer biology. Here, we discuss current knowledge regarding the role of subpopulations of EVs in cancer development and progression and highlight the relevance of EV heterogeneity. The position of tetraspanins and integrins therein will be highlighted. Since addressing EV heterogeneity has become essential for the EV field, current and novel Techniques for isolating EV subpopulations will also be discussed. Further dissection of EV heterogeneity will advance our understanding of the critical roles of EVs in health and disease.
Aimery De Gramont - One of the best experts on this subject based on the ideXlab platform.
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Disseminated and circulating tumor cells in gastrointestinal oncology.
Critical Reviews in Oncology Hematology, 2012Co-Authors: François-clément Bidard, François-régis Ferrand, Florence Huguet, Pascal Hammel, Christophe Louvet, David Malka, Valérie Boige, Michel Ducreux, Thierry Andre, Aimery De GramontAbstract:Circulating (CTCs) and disseminated tumor cells (DTCs) are two different steps in the metastatic process. Several recent Techniques have allowed detection of these cells in patients, and have generated many results using different Isolation Techniques in small cohorts. Herein, we review the detection results and their clinical consequence in esophageal, gastric, pancreatic, colorectal, and liver carcinomas, and discuss their possible applications as new biomarkers.
Shiro Dosho - One of the best experts on this subject based on the ideXlab platform.
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Isolation Techniques against substrate noise coupling utilizing through silicon via tsv process for rf mixed signal socs
IEEE Journal of Solid-state Circuits, 2012Co-Authors: Shinichiro Uemura, Yukio Hiraoka, Shiro DoshoAbstract:The Isolation Techniques against substrate noise coupling utilizing through silicon via (TSV) process are described. The trench shape TSV encloses the RF circuit on a SoC chip to improve the Isolation between digital circuits and the RF circuits without constraints of on-chip interconnect above first metal as the TSV is connected to the grounded 1st metal from the back side of the substrate. The analysis with simplified model is proposed to show the effect of the proposed Isolation Techniques. Mesh circuit model is applied to simulate the noise distribution in detail. Various test patterns are fabricated on a CMOS silicon substrate with resistivity of 10 Ωcm. The measurement pattern of H-shaped TSV confirms about 30 dB and 40 dB improvement at 100 MHz and 1 GHz respectively, which is much better than conventional Isolation Techniques such as guard ring, Deep N-well and DTI. The combinational pattern with TSV, DTI and high resistive layer shows 60 dB improvement of the Isolation. Proposed Isolation Techniques are useful for substrate noise coupling of future RF/mixed-signal SoCs.
Imre Mager - One of the best experts on this subject based on the ideXlab platform.
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extracellular vesicle heterogeneity subpopulations Isolation Techniques and diverse functions in cancer progression
Frontiers in Immunology, 2018Co-Authors: Eduard Willms, Carlos Cabanas, Imre Mager, Matthew J A Wood, Pieter VaderAbstract:Cells release membrane enclosed nano-sized vesicles termed extracellular vesicles (EVs) that function as mediators of intercellular communication by transferring biological information between cells. Tumor-derived EVs have emerged as important mediators in cancer development and progression, mainly through transfer of their bioactive content which can include oncoproteins, oncogenes, chemokine receptors, as well as soluble factors, transcripts of proteins and miRNAs involved in angiogenesis or inflammation. This transfer has been shown to influence the metastatic behavior of primary tumors. Moreover, tumor-derived EVs have been shown to influence distant cellular niches, establishing favorable microenvironments that support growth of disseminated cancer cells upon their arrival at these pre-metastatic niches. It is generally accepted that cells release a number of major EV populations with distinct biophysical properties and biological functions. Exosomes, microvesicles, and apoptotic bodies are EV populations most widely studied and characterized. They are discriminated based primarily on their intracellular origin. However, increasing evidence suggests that even within these EV populations various subpopulations may exist. This heterogeneity introduces an extra level of complexity in the study of EV biology and function. For example, EV subpopulations could have unique roles in the intricate biological processes underlying cancer biology. Here, we discuss current knowledge regarding the role of subpopulations of EVs in cancer development and progression and highlight the relevance of EV heterogeneity. The position of tetraspanins and integrins therein will be highlighted. Since addressing EV heterogeneity has become essential for the EV field, current and novel Techniques for isolating EV subpopulations will also be discussed. Further dissection of EV heterogeneity will advance our understanding of the critical roles of EVs in health and disease.
Carlos Cabanas - One of the best experts on this subject based on the ideXlab platform.
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extracellular vesicle heterogeneity subpopulations Isolation Techniques and diverse functions in cancer progression
Frontiers in Immunology, 2018Co-Authors: Eduard Willms, Carlos Cabanas, Imre Mager, Matthew J A Wood, Pieter VaderAbstract:Cells release membrane enclosed nano-sized vesicles termed extracellular vesicles (EVs) that function as mediators of intercellular communication by transferring biological information between cells. Tumor-derived EVs have emerged as important mediators in cancer development and progression, mainly through transfer of their bioactive content which can include oncoproteins, oncogenes, chemokine receptors, as well as soluble factors, transcripts of proteins and miRNAs involved in angiogenesis or inflammation. This transfer has been shown to influence the metastatic behavior of primary tumors. Moreover, tumor-derived EVs have been shown to influence distant cellular niches, establishing favorable microenvironments that support growth of disseminated cancer cells upon their arrival at these pre-metastatic niches. It is generally accepted that cells release a number of major EV populations with distinct biophysical properties and biological functions. Exosomes, microvesicles, and apoptotic bodies are EV populations most widely studied and characterized. They are discriminated based primarily on their intracellular origin. However, increasing evidence suggests that even within these EV populations various subpopulations may exist. This heterogeneity introduces an extra level of complexity in the study of EV biology and function. For example, EV subpopulations could have unique roles in the intricate biological processes underlying cancer biology. Here, we discuss current knowledge regarding the role of subpopulations of EVs in cancer development and progression and highlight the relevance of EV heterogeneity. The position of tetraspanins and integrins therein will be highlighted. Since addressing EV heterogeneity has become essential for the EV field, current and novel Techniques for isolating EV subpopulations will also be discussed. Further dissection of EV heterogeneity will advance our understanding of the critical roles of EVs in health and disease.
