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Yukari Sotohira - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo Disease (lumpy Jaw Disease; LJD) is a Disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with lumpy Jaw Disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by lumpy Jaw Disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
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Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy Jaw Disease) using an automated handheld testing system
The Journal of veterinary medical science, 2016Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Masakazu Tsuchiya, Toshio Shimamori, Kenji Tsukano, Haruka Sasaki, Yohko Suzuki, Ayano Sato, Hiroshi YokotaAbstract:The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with lumpy Jaw Disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P 0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
Mitsuhiko Asakawa - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo Disease (lumpy Jaw Disease; LJD) is a Disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with lumpy Jaw Disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by lumpy Jaw Disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
Kazuyuki Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos
Journal of Zoo Biology, 2018Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Mitsuhiko Asakawa, Haruna Okui, Tadashi SanoAbstract:Kangaroo Disease (lumpy Jaw Disease; LJD) is a Disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with lumpy Jaw Disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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Stress assessment using hair cortisol of kangaroos affected by the lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Chigusa Arai, Mitsuhiko Asakawa, Hideaki HayashiAbstract:The aim of this study was to objectively assess stress of kangaroos affected by lumpy Jaw Disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
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Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy Jaw Disease) using an automated handheld testing system
The Journal of veterinary medical science, 2016Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Masakazu Tsuchiya, Toshio Shimamori, Kenji Tsukano, Haruka Sasaki, Yohko Suzuki, Ayano Sato, Hiroshi YokotaAbstract:The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with lumpy Jaw Disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P 0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
Samuel Meyrowitz - One of the best experts on this subject based on the ideXlab platform.
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Metastatic tumors in the Jaws: A retrospective study of 114 cases
Journal of the American Dental Association (1939), 2006Co-Authors: Nisha J. D'silva, Don John Summerlin, Kitrina G. Cordell, Rafik A. Abdelsayed, Charles E. Tomich, Carl T. Hanks, Dalbert Fear, Samuel MeyrowitzAbstract:ABSTRACT Background Malignancies involving the bones are metastatic tumors more commonly than primary tumors. In this retrospective study, the authors review metastatic Disease in the Jaws. Methods The authors retrieved cases of metastatic Disease in the Jaws over a 45-year period from the pathology archives at the University of Michigan School of Dentistry, Ann Arbor, and Indiana University School of Dentistry, Indianapolis. Results The authors conducted a retrospective analysis of 114 cases of metastatic Disease in the Jaws and found that approximately 60 percent of subjects had no history of malignancy. The sex distribution was equivalent. Mandibular predilection was more prominent in females than in males. Metastases from the breast were significantly greater than those from the lung and prostate ( P ≤ .05), the second and third most frequent sites, respectively. Women exhibited twice as many Jaw metastases as did men 31 to 40 years of age and significantly fewer metastases than did men 71 to 80 years of age ( P ≤ .05). Conclusion In the majority of cases, subjects had an undiagnosed primary cancer at the time the metastatic Jaw Disease presented. The most common site of origin of the primary cancer was the breast, when primary sites were considered independent of sex. Clinical Implications Patients with metastatic Disease in the Jaws may have innocuous dental symptoms, such as pulpal or periodontal pain; therefore, clinicians will play a significant role in diagnosing the life-threatening Disease.
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Metastatic tumors to the Jaws: retrospective study of 101 cases
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 2004Co-Authors: Nisha J. D'silva, Don John Summerlin, Rafik A. Abdelsayed, Charles E. Tomich, Carl T. Hanks, K.c. Cordell, Samuel MeyrowitzAbstract:Background Metastatic tumors to the Jaws are rare lesions that often present with innocuous symptoms mimicking dental infection. In this study, we report 2 cases of prostatic adenocarcinoma metastatic to the mandible and review 101 cases of metastatic cancer from the archives of the oral pathology biopsy services of the University of Michigan and Indiana University Schools of Dentistry. Study design After IRB approval at both institutions, cases diagnosed as metastatic Disease to the Jaws were retrieved. Data collected from these cases included gender, age, clinical signs and symptoms, location, and primary tumor diagnosis. Case 1 The lesion presented as an expansile bony mass in the mandible of a 76-year-old male with a history of prostatic adenocarcinoma. Case 2 An 82-year-male with a history of prostatic adenocarcinoma presented with a lesion causing bone destruction and swelling of the mandibular alveolar ridge. Results In a retrospective analysis of archival cases there was an equivalent gender distribution and a 5:1 mandibular predilection. The breast and the lungs were the most common primary tumor sites for females and males, respectively. When all metastatic tumors were considered, regardless of gender or location, the breast was the most common site of origin. Pain was the most common presenting symptom, followed by paresthesia. There was a wide age distribution, but women exhibited twice as many Jaw metastases as men in the 31-40 age group and a third as many in the 71-80 age group. Conclusion Metastatic Jaw Disease presents at an earlier age in women than in men and exhibits an equivalent distribution in both genders. Patients presenting with dental symptoms and with a history of malignancy should be evaluated for the possibility of metastatic Disease.
Kenji Tsukano - One of the best experts on this subject based on the ideXlab platform.
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Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy Jaw Disease.
The Journal of veterinary medical science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P
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plasma endotoxin activity in eastern grey kangaroos macropus giganteus with lumpy Jaw Disease
Journal of Veterinary Medical Science, 2017Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Marina Otsuka, Masakazu Tsuchiya, Toshio Shimamori, Yasunobu Nishi, Kenji Tsukano, Mitsuhiko AsakawaAbstract:Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy Jaw Disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
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Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy Jaw Disease) using an automated handheld testing system
The Journal of veterinary medical science, 2016Co-Authors: Yukari Sotohira, Kazuyuki Suzuki, Tadashi Sano, Masakazu Tsuchiya, Toshio Shimamori, Kenji Tsukano, Haruka Sasaki, Yohko Suzuki, Ayano Sato, Hiroshi YokotaAbstract:The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with lumpy Jaw Disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P 0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
