The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
J C Rambaud - One of the best experts on this subject based on the ideXlab platform.
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immunological study of histologically non involved jejunum during crohn s disease evidence for reduced in vivo Secretion of secretory iga
Clinical and Experimental Immunology, 2008Co-Authors: P Marteau, Jf Colombel, J Nemeth, Jeanpierre Vaerman, J C Dive, J C RambaudAbstract:We studied the local humoral immunity of histologically non-involved jejunum in patients with Crohn's disease. Normal subjects and patients with ulcerative colitis served as controls. Jejunal fluid samples were collected during segmental Jejunal perfusion, under an occluding balloon and the in vivo Jejunal Secretion of the following proteins was determined: albumin, orosomucoid, transferrin, alpha 2-macroglobulin, secretory component, monomeric and polymeric IgA, IgG, and IgM. The densities and number of IgA-, IgG-, and IgM-containing cells in the lamina propria of the jejunum were measured on perendoscopic biopsies. Jejunal Secretion of polymeric IgA and the density of IgA-containing plasma cells in the lamina propria were significantly lower in patients with Crohn's disease than in both control groups. This abnormal intestinal immune response, which was not correlated to the activity of the disease, might be involved in its pathogenesis.
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human Jejunal Secretion induced by prostaglandin e1 a dose response study
British Journal of Clinical Pharmacology, 1991Co-Authors: Iradj Sobhani, N Vidon, B Huchet, J C RambaudAbstract:1. Intraluminally infused prostaglandins induce Jejunal Secretion of water and electrolytes in man, and a receptor-mediated process in the intestinal epithelial cells has been suggested to explain this Secretion. In an attempt to obtain data under basal conditions for pharmacological studies, we tested the dose-response effect of PGE1 on Jejunal hydroelectrolytic movements in 10 healthy volunteers. 2. Accordingly, a solution with or without PGE1 was infused via a four-lumen tube with a proximal occluding balloon and Jejunal water and electrolyte transport were determined. The segment tested was 40 cm long. Seven randomized doses of PGE1 ranging from 0.01 to 1.3 micrograms kg-1 min-1 (2.83 to 364 pmol kg-1 min-1) were infused in an isotonic control saline solution. The Secretion induced by PGE1 was evaluated in each subject as the difference between fluxes in response to the control saline solution and to PGE1. 3. Whatever the dose, PGE1 induced Secretion of water, Na+, K+ and Cl- which was dose-dependent and saturable, with a mean Km of congruent to 6-8 pmol kg-1 min-1, suggesting that at the pharmacological doses used, enterocytes have a saturable membrane site similar to a single class of receptor for PGE1. 4. These findings may be of great importance if prostaglandins are administered as drugs or used as Jejunal secretory agents in vivo when the antisecretory effect of another drug is studied.
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Jejunal immunoglobulin Secretion in alcoholic patients with and without cirrhosis
Journal of Hepatology, 1991Co-Authors: Jeanfrederic Colombel, Jean-pierre Dehennin, Jeanpierre Vaerman, Charles Dive, B Mesnard, J C RambaudAbstract:Alcoholic liver diseases are associated with an elevation of serum immunoglobulin A (IgA). This could be the result of increased IgA production by the intestinal mucosa. Serum and Jejunal immunoglobulin, albumin and orosomucoid were measured in 13 alcoholic patients with (n = 6) and without (n = 7) cirrhosis and compared to 11 controls. Jejunal Secretions were obtained by segmental perfusion under an occluding balloon. High levels of serum monomeric and polymeric IgA were only found in patients with cirrhosis. Alcoholics with and without cirrhosis had normal monomeric and polymeric IgA Jejunal Secretion rates. Jejunal clearances of albumin, orosomucoid and immunoglobulin G were significantly higher in both non-cirrhotic and cirrhotic patients than in controls. These findings indicate normal Jejunal IgA Secretion and increased permeability to plasma proteins, such as albumin and immunoglobulin G in alcoholics.
Iradj Sobhani - One of the best experts on this subject based on the ideXlab platform.
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lanreotide inhibits human Jejunal Secretion induced by prostaglandin el in healthy volunteers
British Journal of Clinical Pharmacology, 1996Co-Authors: Iradj Sobhani, Emmanuel Rene Akli Ramdani, Faysel Bayod, Luis Sabbagh, Francois Thomas, M MignonAbstract:1 Somatostatin inhibits hormonal Secretions in the gastrointestinal tract. Somatostatin analogues are used in the treatment of VIPome-related watery diarrhoea. In addition, more than 10% of patients with AIDS suffer from diarrhoea likely due to the increased intestinal Secretion of water and ions. However, the direct effect of somatostatin on the flux of water and ions in the intestine has not been, so far, analyzed in vivo. The aim of the present study was to evaluate the effect of lanreotide, a somatostatin analogue, on the movements of water and ions in the jejunum in man. 2 Accordingly, 10 healthy volunteers (age 18–35 years, mean 27) and two patients with AIDS (26 and 33 years) suffering from water diarrhoea (> 800 ml day-1) underwent intestinal perfusion using a four lumen tube with proximal occluding balloon. The segment tested was 25 cm long. The jejunum was infused by an isotonic control saline solution containing polyethylene glycol (PEG) as non-absorbable marker. Basal Jejunal Secretions were measured in all subjects. Prostaglandin El (PGE1) was administered intraluminally to stimulate Jejunal Secretion in healthy volunteers. The effect of intravenous lanreotide on the Jejunal PGE1-induced Secretions of water and electrolytes was analysed in healthy subjects and on the basal Secretions in AIDS patients. Each period was analyzed on the basis of three (10 min) successive intestinal juice collections after 20–30 min equilibration time. The antisecretory effect of lanreotide was evaluated in each subject as the difference between fluxes compared to the control period. 3 In healthy volunteers, PGE1 induced Secretion of H2O, Na+, K+ and C1- in the jejunum and lanreotide reduced significantly PGE1-induced response. In both AIDS patients basal fluxes of water and ions were reduced by lanreotide in a dose-dependent manner. 4 Somatostatin can reduce stimulated-Jejunal Secretion of ions and water in normal subjects and may improve water diarrhoea in AIDS patients.
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human Jejunal Secretion induced by prostaglandin e1 a dose response study
British Journal of Clinical Pharmacology, 1991Co-Authors: Iradj Sobhani, N Vidon, B Huchet, J C RambaudAbstract:1. Intraluminally infused prostaglandins induce Jejunal Secretion of water and electrolytes in man, and a receptor-mediated process in the intestinal epithelial cells has been suggested to explain this Secretion. In an attempt to obtain data under basal conditions for pharmacological studies, we tested the dose-response effect of PGE1 on Jejunal hydroelectrolytic movements in 10 healthy volunteers. 2. Accordingly, a solution with or without PGE1 was infused via a four-lumen tube with a proximal occluding balloon and Jejunal water and electrolyte transport were determined. The segment tested was 40 cm long. Seven randomized doses of PGE1 ranging from 0.01 to 1.3 micrograms kg-1 min-1 (2.83 to 364 pmol kg-1 min-1) were infused in an isotonic control saline solution. The Secretion induced by PGE1 was evaluated in each subject as the difference between fluxes in response to the control saline solution and to PGE1. 3. Whatever the dose, PGE1 induced Secretion of water, Na+, K+ and Cl- which was dose-dependent and saturable, with a mean Km of congruent to 6-8 pmol kg-1 min-1, suggesting that at the pharmacological doses used, enterocytes have a saturable membrane site similar to a single class of receptor for PGE1. 4. These findings may be of great importance if prostaglandins are administered as drugs or used as Jejunal secretory agents in vivo when the antisecretory effect of another drug is studied.
M J G Farthing - One of the best experts on this subject based on the ideXlab platform.
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the sigma ligand igmesine inhibits cholera toxin and escherichia coli enterotoxin induced Jejunal Secretion in the rat
Gut, 1999Co-Authors: J L Turvill, P Kasapidis, M J G FarthingAbstract:BACKGROUND—Cholera toxin, and Escherichia coli heat labile (LT) and heat stable (STa) enterotoxins induce small intestinal Secretion in part by activating enteric nerves. Igmesine is a novel sigma receptor ligand that inhibits neurally mediated Secretion. AIMS—To assess the antisecretory potential of igmesine in cholera toxin, LT, and STa induced water and electrolyte Secretion using an in vivo rat model of Jejunal perfusion. METHODS—After pretreatment with igmesine, 0.03-10 mg/kg intravenously, Jejunal segments of anaesthetised, adult male Wistar rats were incubated with cholera toxin (25 µg), LT (25 µg), or saline. Jejunal perfusion with a plasma electrolyte solution containing a non-absorbable marker was undertaken. In some cases 200 µg/l STa was added to the perfusate. After equilibration, net water and electrolyte movement was determined. In additional experiments rats received igmesine, intravenously or intraJejunally, after exposure to cholera toxin. RESULTS—Cholera toxin induced net water Secretion was inhibited by 1 mg/kg igmesine (median −120 versus −31 µl/min/g, p<0.001). LT and STa induced Secretion were also inhibited by 1 mg/kg igmesine (−90 versus −56, p<0.03; and −76 versus −29, p<0.01, respectively). Igmesine reduced established cholera toxin induced Secretion. CONCLUSION—The sigma ligand, igmesine, inhibits neurally mediated enterotoxigenic Secretion. Its ability to inhibit established Secretion makes it an agent with therapeutic potential. Keywords: igmesine; cholera toxin; Escherichia coli enterotoxin; Jejunal Secretion
M Mignon - One of the best experts on this subject based on the ideXlab platform.
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lanreotide inhibits human Jejunal Secretion induced by prostaglandin el in healthy volunteers
British Journal of Clinical Pharmacology, 1996Co-Authors: Iradj Sobhani, Emmanuel Rene Akli Ramdani, Faysel Bayod, Luis Sabbagh, Francois Thomas, M MignonAbstract:1 Somatostatin inhibits hormonal Secretions in the gastrointestinal tract. Somatostatin analogues are used in the treatment of VIPome-related watery diarrhoea. In addition, more than 10% of patients with AIDS suffer from diarrhoea likely due to the increased intestinal Secretion of water and ions. However, the direct effect of somatostatin on the flux of water and ions in the intestine has not been, so far, analyzed in vivo. The aim of the present study was to evaluate the effect of lanreotide, a somatostatin analogue, on the movements of water and ions in the jejunum in man. 2 Accordingly, 10 healthy volunteers (age 18–35 years, mean 27) and two patients with AIDS (26 and 33 years) suffering from water diarrhoea (> 800 ml day-1) underwent intestinal perfusion using a four lumen tube with proximal occluding balloon. The segment tested was 25 cm long. The jejunum was infused by an isotonic control saline solution containing polyethylene glycol (PEG) as non-absorbable marker. Basal Jejunal Secretions were measured in all subjects. Prostaglandin El (PGE1) was administered intraluminally to stimulate Jejunal Secretion in healthy volunteers. The effect of intravenous lanreotide on the Jejunal PGE1-induced Secretions of water and electrolytes was analysed in healthy subjects and on the basal Secretions in AIDS patients. Each period was analyzed on the basis of three (10 min) successive intestinal juice collections after 20–30 min equilibration time. The antisecretory effect of lanreotide was evaluated in each subject as the difference between fluxes compared to the control period. 3 In healthy volunteers, PGE1 induced Secretion of H2O, Na+, K+ and C1- in the jejunum and lanreotide reduced significantly PGE1-induced response. In both AIDS patients basal fluxes of water and ions were reduced by lanreotide in a dose-dependent manner. 4 Somatostatin can reduce stimulated-Jejunal Secretion of ions and water in normal subjects and may improve water diarrhoea in AIDS patients.
Jf Colombel - One of the best experts on this subject based on the ideXlab platform.
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immunological study of histologically non involved jejunum during crohn s disease evidence for reduced in vivo Secretion of secretory iga
Clinical and Experimental Immunology, 2008Co-Authors: P Marteau, Jf Colombel, J Nemeth, Jeanpierre Vaerman, J C Dive, J C RambaudAbstract:We studied the local humoral immunity of histologically non-involved jejunum in patients with Crohn's disease. Normal subjects and patients with ulcerative colitis served as controls. Jejunal fluid samples were collected during segmental Jejunal perfusion, under an occluding balloon and the in vivo Jejunal Secretion of the following proteins was determined: albumin, orosomucoid, transferrin, alpha 2-macroglobulin, secretory component, monomeric and polymeric IgA, IgG, and IgM. The densities and number of IgA-, IgG-, and IgM-containing cells in the lamina propria of the jejunum were measured on perendoscopic biopsies. Jejunal Secretion of polymeric IgA and the density of IgA-containing plasma cells in the lamina propria were significantly lower in patients with Crohn's disease than in both control groups. This abnormal intestinal immune response, which was not correlated to the activity of the disease, might be involved in its pathogenesis.
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effect of intraJejunal elemental diet perfusion on Jejunal Secretion of immunoglobulins albumin and hyaluronan in man
Gut, 1992Co-Authors: Jf Colombel, Jean-pierre Dehennin, Jp Vaerman, R Hallgren, E Wain, R Modigliani, A CortotAbstract:The aim of this work was to study the Jejunal Secretion of immunoglobulins (Ig), albumin, and hyaluronan in response to Jejunal perfusion of an elemental diet. A four lumen tube with a proximal occluding balloon at the angle of Treitz was used for Jejunal perfusion in seven healthy volunteers (mean age 23 years). The length of the test segment was 40 cm. The jejunum was successively perfused with a control electrolyte solution for 80 minutes and with an elemental diet (containing 20.5 milligrams of free amino acids and 104.2 milligrams of oligosaccharides) for 100 minutes. The Jejunal fluid concentrations of albumin, IgG, monomeric IgA (m-IgA), polymeric IgA (p-IgA), IgM, secretory component, and hyaluronan were measured and their Jejunal outputs calculated. Within 20 minutes of starting perfusion with the elemental diet there was a significant increase in the Secretion rates of albumin (x3.3), IgG (x5), M-IgA (x3.7), p-IgA (x2), IgM (x2), and secretory component (x1.6), but the hyaluronan Secretion rate was not changed. The increase in m-IgA, p-IgA, IgM, and secretory component output suggests that intestinal perfusion of an elemental diet results in stimulation of secretory immunity. The increase in albumin and IgG output probably reflects a nutrient induced leakage from the plasma compartment.