Jungle Fowl

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Elisabeth Blesbois - One of the best experts on this subject based on the ideXlab platform.

  • effect of glutathione on pre and post freezing sperm quality of indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2021
    Co-Authors: M S Ansari, Shamim Akhter, Elisabeth Blesbois, Ali Akhter, B A Rakha, J Santiagomoreno
    Abstract:

    During cryopreservation sperm encounter oxidative stress due to higher production of ROS molecules and insufficient natural antioxidant defence system. Therefore, present study was designed to identify the effects of various glutathione (GSH) concentrations on Indian red Jungle Fowl (Gallus gallus murghi) sperm quality and fertility pre-freezing and post-thaw incubation hours. Semen was collected from eight cocks and qualified semen ejaculates having motility >65% were pooled after initial evaluation. Semen was divided in four aliquots, diluted with red Fowl extender (1:5) at 37 °C having GSH 0 mM (control), 0.1 mM, 0.5 mM and 1.0 mM, cryopreserved and stored at (-196 °C) in liquid nitrogen. Semen quality was assessed at post dilution, cooling, equilibration, and freeze-thawing at 0, 2 and 4 h of incubation at 37 °C. Sperm motility, plasma membrane integrity, viability, acrosome integrity and mitochondrial function were recorded highest (P < 0.05) with 0.5 mM GSH in extender at post-dilution, cooling, equilibration, freeze-thawing and 0, 2 and 4 h of incubation. Lipid peroxidation in sperm and seminal plasma were recorded lowest (P < 0.05) with 0.5 mM GSH during cryopreservation stages and post-thawing incubation. Moreover, antioxidant activities (total antioxidant potential and free radical scavenging capacity) were recorded highest (P < 0.05) in extender having 0.5 mM GSH. Fertility rates were recorded higher (P < 0.05) with 0.5 mM GSH compared to control. It is concluded that 0.5 mM GSH in extender improves sperm structural (sperm viability, plasma membrane integrity and acrosome integrity), functional integrity (motility, mitochondrial function) and fertility parameters of Indian red Jungle Fowl through enriching antioxidant potential and ameliorating the oxidative stress.

  • effect of dimethylformamide on sperm quality and fertilizing ability of indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2020
    Co-Authors: B A Rakha, Shamim Akhter, Elisabeth Blesbois, Ali Akhter, M S Ansari, J Santiagomoreno
    Abstract:

    Abstract The present study investigates the efficacy of dimehtlyformamide (DMF) as a permeable cryoprotectant and its effect on quality and fertility of Indian red Jungle Fowl sperm. Semen was collected from eight mature roosters, pooled, divided into five aliquots and diluted with red Fowl extender having DMF (0%, 4%, 6%, 8% and 10%). Diluted semen samples were cooled from 37 °C to 4 °C, 20% glycerol added to control (0% DMF), equilibrated for 10 min and filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Sperm motility, plasma membrane functionality, viability and acrosome integrity were assessed at post dilution, cooling, equilibration and freeze-thawing stage of cryopreservation. Cryopreservation stages had negative effects (P

  • effect of cryopreservation on lipid peroxidation antioxidant potential chromatin integrity and mitochondrial activity of indian red Jungle Fowl gallus gallus murghi semen
    Biopreservation and Biobanking, 2019
    Co-Authors: Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter, Elisabeth Blesbois, J Santiagomoreno
    Abstract:

    Aim: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red Jungle Fowl sperm. Materials and...

  • use of dimethylsulfoxide for semen cryopreservation in indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Elisabeth Blesbois, Iftikhar Hussain, Z. Zafar, A. Naseer, J Santiagomoreno
    Abstract:

    Abstract Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating cryoprotectant for biological cells, has been recognized as an adequate alternative for cryopreservation of sperm from several species. This study was designed to evaluate the efficacy of different concentrations of DMSO as cryoprotectant for Indian red Jungle Fowl (Gallus gallus murghi) sperm. Semen was collected from Indian red Jungle Fowl cocks, pooled and divided into five aliquots. Different concentrations of DMSO (0%, 4%, 6%, 8% and 10%) were compared. Diluted semen was cooled from 37 °C to 4 °C (−0.275 °C min−1), 20% glycerol added to control and equilibrated for 10 min. After equilibration, semen was filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Semen samples were thawed at 37 °C for 30 s. Cryo-survival of Indian red Jungle Fowl sperm was affected by cryopreservation stages and different concentrations of cryoprotectant used. Highest sperm motility (85.0 ± 2.9; 80.0 ± 3.5; 71.3 ± 4.3; 60.0 ± 1.3), plasma membrane integrity (79.5 ± 3.8; 75.3 ± 2.4; 72.8 ± 3.3; 60.3 ± 2.8), viability (80.8 ± 4.6; 75.5 ± 2.9; 71.0 ± 7.6; 58.8 ± 1.3) and acrosomal integrity (76.3 ± 2.4; 72.0 ± 6.0; 62.5 ± 4.3; 55.0 ± 3.2) were recorded in a diluent having 8% DMSO at post-dilution, cooling, equilibration and freeze-thawing. Highest fertility results were obtained after artificial insemination with 8% DMSO compared to 20% glycerol (73.0 ± 4.4 vs 53.1 ± 4.3, P

  • Cryoprotectant effects of egg yolk on Indian red Jungle Fowl (Gallus gallus murghi) sperm
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, J. Santiago-moreno, Elisabeth Blesbois
    Abstract:

    Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red Jungle Fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red Jungle Fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 +/- 2.5%), plasma membrane integrity (663 +/- 2.4%), viability (58.8 +/- 1.3%) and acrosomal integrity (60.0.8 +/- 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 +/- 0.7, 21.6 +/- 1.2), percent fertility (55.9 +/- 4.4, 46.5 +/- 2.2), no. of hatched chicks (23.6 +/- 1.0,17.2 +/- 1.0), percent hatch (49.5 +/- 3.2, 37.1 +/- 2.5%) and hatchability of the fertile eggs (89.4 +/- 2.2, 79.7 +/- 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red Jungle Fowl sperm.

Bushra Allah Rakha - One of the best experts on this subject based on the ideXlab platform.

  • effect of quercetin on oxidative stress mitochondrial activity and quality of indian red Jungle Fowl gallus gallus murghi sperm
    Biopreservation and Biobanking, 2020
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Ali Akhter, Muhammad Amjad Awan, J Santiagomoreno
    Abstract:

    Aim: The study was designed to elucidate the effects of quercetin in an extender on oxidative stress, mitochondrial activity and quality of Indian red Jungle Fowl (Gallus gallus murghi) sperm durin...

  • effect of cryopreservation on lipid peroxidation antioxidant potential chromatin integrity and mitochondrial activity of indian red Jungle Fowl gallus gallus murghi semen
    Biopreservation and Biobanking, 2019
    Co-Authors: Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter, Elisabeth Blesbois, J Santiagomoreno
    Abstract:

    Aim: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red Jungle Fowl sperm. Materials and...

  • use of dimethylsulfoxide for semen cryopreservation in indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Elisabeth Blesbois, Iftikhar Hussain, Z. Zafar, A. Naseer, J Santiagomoreno
    Abstract:

    Abstract Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating cryoprotectant for biological cells, has been recognized as an adequate alternative for cryopreservation of sperm from several species. This study was designed to evaluate the efficacy of different concentrations of DMSO as cryoprotectant for Indian red Jungle Fowl (Gallus gallus murghi) sperm. Semen was collected from Indian red Jungle Fowl cocks, pooled and divided into five aliquots. Different concentrations of DMSO (0%, 4%, 6%, 8% and 10%) were compared. Diluted semen was cooled from 37 °C to 4 °C (−0.275 °C min−1), 20% glycerol added to control and equilibrated for 10 min. After equilibration, semen was filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Semen samples were thawed at 37 °C for 30 s. Cryo-survival of Indian red Jungle Fowl sperm was affected by cryopreservation stages and different concentrations of cryoprotectant used. Highest sperm motility (85.0 ± 2.9; 80.0 ± 3.5; 71.3 ± 4.3; 60.0 ± 1.3), plasma membrane integrity (79.5 ± 3.8; 75.3 ± 2.4; 72.8 ± 3.3; 60.3 ± 2.8), viability (80.8 ± 4.6; 75.5 ± 2.9; 71.0 ± 7.6; 58.8 ± 1.3) and acrosomal integrity (76.3 ± 2.4; 72.0 ± 6.0; 62.5 ± 4.3; 55.0 ± 3.2) were recorded in a diluent having 8% DMSO at post-dilution, cooling, equilibration and freeze-thawing. Highest fertility results were obtained after artificial insemination with 8% DMSO compared to 20% glycerol (73.0 ± 4.4 vs 53.1 ± 4.3, P

  • Cryoprotectant effects of egg yolk on Indian red Jungle Fowl (Gallus gallus murghi) sperm
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, J. Santiago-moreno, Elisabeth Blesbois
    Abstract:

    Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red Jungle Fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red Jungle Fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 +/- 2.5%), plasma membrane integrity (663 +/- 2.4%), viability (58.8 +/- 1.3%) and acrosomal integrity (60.0.8 +/- 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 +/- 0.7, 21.6 +/- 1.2), percent fertility (55.9 +/- 4.4, 46.5 +/- 2.2), no. of hatched chicks (23.6 +/- 1.0,17.2 +/- 1.0), percent hatch (49.5 +/- 3.2, 37.1 +/- 2.5%) and hatchability of the fertile eggs (89.4 +/- 2.2, 79.7 +/- 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red Jungle Fowl sperm.

  • Cryopreservation of Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa: A First Approach:
    Avian Biology Research, 2017
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Syed Qasim Raza, Iftikhar Hussain, Maqsood Anwar, Elisabeth Blesbois
    Abstract:

    The experiment was designed to show the capacity of Indian Red Jungle Fowl (Gallus gallus murghi) sperm to be cryopreserved. The changes in characteristics of Indian Red Jungle Fowl spermatozoa dur...

Shamim Akhter - One of the best experts on this subject based on the ideXlab platform.

  • effect of glutathione on pre and post freezing sperm quality of indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2021
    Co-Authors: M S Ansari, Shamim Akhter, Elisabeth Blesbois, Ali Akhter, B A Rakha, J Santiagomoreno
    Abstract:

    During cryopreservation sperm encounter oxidative stress due to higher production of ROS molecules and insufficient natural antioxidant defence system. Therefore, present study was designed to identify the effects of various glutathione (GSH) concentrations on Indian red Jungle Fowl (Gallus gallus murghi) sperm quality and fertility pre-freezing and post-thaw incubation hours. Semen was collected from eight cocks and qualified semen ejaculates having motility >65% were pooled after initial evaluation. Semen was divided in four aliquots, diluted with red Fowl extender (1:5) at 37 °C having GSH 0 mM (control), 0.1 mM, 0.5 mM and 1.0 mM, cryopreserved and stored at (-196 °C) in liquid nitrogen. Semen quality was assessed at post dilution, cooling, equilibration, and freeze-thawing at 0, 2 and 4 h of incubation at 37 °C. Sperm motility, plasma membrane integrity, viability, acrosome integrity and mitochondrial function were recorded highest (P < 0.05) with 0.5 mM GSH in extender at post-dilution, cooling, equilibration, freeze-thawing and 0, 2 and 4 h of incubation. Lipid peroxidation in sperm and seminal plasma were recorded lowest (P < 0.05) with 0.5 mM GSH during cryopreservation stages and post-thawing incubation. Moreover, antioxidant activities (total antioxidant potential and free radical scavenging capacity) were recorded highest (P < 0.05) in extender having 0.5 mM GSH. Fertility rates were recorded higher (P < 0.05) with 0.5 mM GSH compared to control. It is concluded that 0.5 mM GSH in extender improves sperm structural (sperm viability, plasma membrane integrity and acrosome integrity), functional integrity (motility, mitochondrial function) and fertility parameters of Indian red Jungle Fowl through enriching antioxidant potential and ameliorating the oxidative stress.

  • effect of quercetin on oxidative stress mitochondrial activity and quality of indian red Jungle Fowl gallus gallus murghi sperm
    Biopreservation and Biobanking, 2020
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Ali Akhter, Muhammad Amjad Awan, J Santiagomoreno
    Abstract:

    Aim: The study was designed to elucidate the effects of quercetin in an extender on oxidative stress, mitochondrial activity and quality of Indian red Jungle Fowl (Gallus gallus murghi) sperm durin...

  • effect of dimethylformamide on sperm quality and fertilizing ability of indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2020
    Co-Authors: B A Rakha, Shamim Akhter, Elisabeth Blesbois, Ali Akhter, M S Ansari, J Santiagomoreno
    Abstract:

    Abstract The present study investigates the efficacy of dimehtlyformamide (DMF) as a permeable cryoprotectant and its effect on quality and fertility of Indian red Jungle Fowl sperm. Semen was collected from eight mature roosters, pooled, divided into five aliquots and diluted with red Fowl extender having DMF (0%, 4%, 6%, 8% and 10%). Diluted semen samples were cooled from 37 °C to 4 °C, 20% glycerol added to control (0% DMF), equilibrated for 10 min and filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Sperm motility, plasma membrane functionality, viability and acrosome integrity were assessed at post dilution, cooling, equilibration and freeze-thawing stage of cryopreservation. Cryopreservation stages had negative effects (P

  • effect of cryopreservation on lipid peroxidation antioxidant potential chromatin integrity and mitochondrial activity of indian red Jungle Fowl gallus gallus murghi semen
    Biopreservation and Biobanking, 2019
    Co-Authors: Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter, Elisabeth Blesbois, J Santiagomoreno
    Abstract:

    Aim: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red Jungle Fowl sperm. Materials and...

  • use of dimethylsulfoxide for semen cryopreservation in indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Elisabeth Blesbois, Iftikhar Hussain, Z. Zafar, A. Naseer, J Santiagomoreno
    Abstract:

    Abstract Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating cryoprotectant for biological cells, has been recognized as an adequate alternative for cryopreservation of sperm from several species. This study was designed to evaluate the efficacy of different concentrations of DMSO as cryoprotectant for Indian red Jungle Fowl (Gallus gallus murghi) sperm. Semen was collected from Indian red Jungle Fowl cocks, pooled and divided into five aliquots. Different concentrations of DMSO (0%, 4%, 6%, 8% and 10%) were compared. Diluted semen was cooled from 37 °C to 4 °C (−0.275 °C min−1), 20% glycerol added to control and equilibrated for 10 min. After equilibration, semen was filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Semen samples were thawed at 37 °C for 30 s. Cryo-survival of Indian red Jungle Fowl sperm was affected by cryopreservation stages and different concentrations of cryoprotectant used. Highest sperm motility (85.0 ± 2.9; 80.0 ± 3.5; 71.3 ± 4.3; 60.0 ± 1.3), plasma membrane integrity (79.5 ± 3.8; 75.3 ± 2.4; 72.8 ± 3.3; 60.3 ± 2.8), viability (80.8 ± 4.6; 75.5 ± 2.9; 71.0 ± 7.6; 58.8 ± 1.3) and acrosomal integrity (76.3 ± 2.4; 72.0 ± 6.0; 62.5 ± 4.3; 55.0 ± 3.2) were recorded in a diluent having 8% DMSO at post-dilution, cooling, equilibration and freeze-thawing. Highest fertility results were obtained after artificial insemination with 8% DMSO compared to 20% glycerol (73.0 ± 4.4 vs 53.1 ± 4.3, P

Muhammad Sajjad Ansari - One of the best experts on this subject based on the ideXlab platform.

  • effect of quercetin on oxidative stress mitochondrial activity and quality of indian red Jungle Fowl gallus gallus murghi sperm
    Biopreservation and Biobanking, 2020
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Ali Akhter, Muhammad Amjad Awan, J Santiagomoreno
    Abstract:

    Aim: The study was designed to elucidate the effects of quercetin in an extender on oxidative stress, mitochondrial activity and quality of Indian red Jungle Fowl (Gallus gallus murghi) sperm durin...

  • effect of cryopreservation on lipid peroxidation antioxidant potential chromatin integrity and mitochondrial activity of indian red Jungle Fowl gallus gallus murghi semen
    Biopreservation and Biobanking, 2019
    Co-Authors: Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter, Elisabeth Blesbois, J Santiagomoreno
    Abstract:

    Aim: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red Jungle Fowl sperm. Materials and...

  • use of dimethylsulfoxide for semen cryopreservation in indian red Jungle Fowl gallus gallus murghi
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Elisabeth Blesbois, Iftikhar Hussain, Z. Zafar, A. Naseer, J Santiagomoreno
    Abstract:

    Abstract Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating cryoprotectant for biological cells, has been recognized as an adequate alternative for cryopreservation of sperm from several species. This study was designed to evaluate the efficacy of different concentrations of DMSO as cryoprotectant for Indian red Jungle Fowl (Gallus gallus murghi) sperm. Semen was collected from Indian red Jungle Fowl cocks, pooled and divided into five aliquots. Different concentrations of DMSO (0%, 4%, 6%, 8% and 10%) were compared. Diluted semen was cooled from 37 °C to 4 °C (−0.275 °C min−1), 20% glycerol added to control and equilibrated for 10 min. After equilibration, semen was filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Semen samples were thawed at 37 °C for 30 s. Cryo-survival of Indian red Jungle Fowl sperm was affected by cryopreservation stages and different concentrations of cryoprotectant used. Highest sperm motility (85.0 ± 2.9; 80.0 ± 3.5; 71.3 ± 4.3; 60.0 ± 1.3), plasma membrane integrity (79.5 ± 3.8; 75.3 ± 2.4; 72.8 ± 3.3; 60.3 ± 2.8), viability (80.8 ± 4.6; 75.5 ± 2.9; 71.0 ± 7.6; 58.8 ± 1.3) and acrosomal integrity (76.3 ± 2.4; 72.0 ± 6.0; 62.5 ± 4.3; 55.0 ± 3.2) were recorded in a diluent having 8% DMSO at post-dilution, cooling, equilibration and freeze-thawing. Highest fertility results were obtained after artificial insemination with 8% DMSO compared to 20% glycerol (73.0 ± 4.4 vs 53.1 ± 4.3, P

  • Cryoprotectant effects of egg yolk on Indian red Jungle Fowl (Gallus gallus murghi) sperm
    Theriogenology, 2018
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, J. Santiago-moreno, Elisabeth Blesbois
    Abstract:

    Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red Jungle Fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red Jungle Fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 +/- 2.5%), plasma membrane integrity (663 +/- 2.4%), viability (58.8 +/- 1.3%) and acrosomal integrity (60.0.8 +/- 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 +/- 0.7, 21.6 +/- 1.2), percent fertility (55.9 +/- 4.4, 46.5 +/- 2.2), no. of hatched chicks (23.6 +/- 1.0,17.2 +/- 1.0), percent hatch (49.5 +/- 3.2, 37.1 +/- 2.5%) and hatchability of the fertile eggs (89.4 +/- 2.2, 79.7 +/- 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red Jungle Fowl sperm.

  • Cryopreservation of Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa: A First Approach:
    Avian Biology Research, 2017
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Syed Qasim Raza, Iftikhar Hussain, Maqsood Anwar, Elisabeth Blesbois
    Abstract:

    The experiment was designed to show the capacity of Indian Red Jungle Fowl (Gallus gallus murghi) sperm to be cryopreserved. The changes in characteristics of Indian Red Jungle Fowl spermatozoa dur...

Maqsood Anwar - One of the best experts on this subject based on the ideXlab platform.

  • Cryopreservation of Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa: A First Approach:
    Avian Biology Research, 2017
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Syed Qasim Raza, Iftikhar Hussain, Maqsood Anwar, Elisabeth Blesbois
    Abstract:

    The experiment was designed to show the capacity of Indian Red Jungle Fowl (Gallus gallus murghi) sperm to be cryopreserved. The changes in characteristics of Indian Red Jungle Fowl spermatozoa dur...

  • Comparison of Extenders for Liquid Storage of Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa
    Avian Biology Research, 2016
    Co-Authors: Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Iftikhar Hussain, Maqsood Anwar, Elisabeth Blesbois
    Abstract:

    This study was designed to evaluate a range of avian semen extenders for liquid storage of Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa at 5 °C. Semen was collected from 8 mature train...

  • breeding ecology of red Jungle Fowl gallus gallus in deva vatala national park azad jammu and kashmir pakistan
    Journal of Applied Agriculture and Biotechnology, 2016
    Co-Authors: Maqsood Anwar, Sakhawat Ali, Muhammad Rais, Tariq Mahmood
    Abstract:

    Red Jungle Fowl (Gallus gallus murghi) was reported for the first time in 2010 from Pakistan in Deva Vatala National Park, Azad Jammu and Kashmir. The present study investigated breeding aspects such as nesting, breeding season, clutch size and hatching success of the species at 10 selected sites in the national park. Nests were mostly found on slopes with dense vegetation cover dominated by Lantana camara and Adhatoda zeylanica. The nesting material consisted of dry leaves, downy feathers, and twigs of plants. The nests were found at sloppy areas as a small depression of 2.5-4 inches in soil made with the help of feet and beak for laying eggs. The breeding season of Red Jungle Fowl expanded from January till July with peak in spring season. Red Jungle Fowl formed harem with dominant male maintaining a territory and reproduced with a group of females throughout the breeding season. Harem formation in Red Jungle Fowl was observed from March to May with average harem size of three females with one male. The average clutch size of Red Jungle Fowl in the study area was 5.5±1.5 eggs per nest. Maximum chicks with hen were observed in June (3.1±0.43) and July (3.3±0.36). The forests in Deva Vatala National Park had thick vegetation cover due to occurrence of heavy rains during Monsoon season, when insects were abundant. The thick cover provided shelter to chicks and insects provide food to them. Our results provide some valuable baseline information on breeding aspects of this least studied species in Pakistan which can be used for its conservation in the park.

  • population status and distribution pattern of red Jungle Fowl gallus gallus murghi in deva vatala national park azad jammu kashmir pakistan a pioneer study
    Pakistan Journal of Zoology, 2010
    Co-Authors: Akram Subhani, Maqsood Anwar, Muhammad Siddique Awan, Usman Ali, Naeem Iftikhar Dar
    Abstract:

    Abstract.- A study was conducted during April- September, 2008 to determine the abundance and distribution of Red Jungle Fowl ( Gallus gallus murghi ) in Deva Vatala National Park, Azad Jammu and Kashmir. Study area was divided in to four localities/sites: Chhumb, Deva, Burmala and Vatala. Line transects and calling site counts methods were used to estimate the population of Red Jungle Fowl at study sites. A total of 34 line transects were walked in study area. A population of 109 individuals of Red Jungle Fowl was estimated in the study area for an overall density of 7.87 birds/km 2 . However, the bird density varied among the sites and was 6.07, 6.25, 8.75 and 15.63 birds/km 2 in Chhumb, Deva, Burmala and Vatala localities, respectively. Red Jungle Fowl occupied densely vegetated, hilly areas between 350m and 415m elevation, especially areas with grassland habitat followed by habitats with Zizyphus mauritiana and Lantana camara dominant vegetation. Red Jas ungle Fowl seem to have pushed back into densely vegetated areas of the park mainly to avoid disturbance by local hunters, shepherds and grass cutters. Results of this pioneer study are important for conservation planning for this important and beautiful species in Deva Vatala National Park.

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