The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Gloria A Moore - One of the best experts on this subject based on the ideXlab platform.
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expression patterns of flowering genes in leaves of pineapple sweet orange citrus sinensis l osbeck and pummelo citrus grandis osb eck
BMC Plant Biology, 2017Co-Authors: Melanie Pajon, Vicente J Febres, Gloria A MooreAbstract:In citrus the transition from Juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of Juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and ‘Pineapple’ sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature ‘Pineapple’ seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions.
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Expression patterns of flowering genes in leaves of ‘Pineapple’ sweet orange [Citrus sinensis (L.) Osbeck] and pummelo (Citrus grandis Osbeck)
BMC, 2017Co-Authors: Melanie Pajon, Vicente J Febres, Gloria A MooreAbstract:Abstract Background In citrus the transition from Juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of Juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). Results In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and ‘Pineapple’ sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature ‘Pineapple’ seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. Conclusions In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions
Masaki Mori - One of the best experts on this subject based on the ideXlab platform.
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the Juvenility associated long noncoding rna gm14230 maintains cellular juvenescence
Journal of Cell Science, 2019Co-Authors: Ayami Tano, Yosuke Kadota, Takao Morimune, Faidruz Azura Jam, Haruka Yukiue, Jeanpierre Bellier, Tatsuyuki Sokoda, Yoshihiro Maruo, Ikuo Tooyama, Masaki MoriAbstract:Juvenile animals possess distinct properties that are missing in adults. These properties include capabilities for higher growth, faster wound healing, plasticity and regeneration. However, the molecular mechanisms underlying these juvenile physiological properties are not fully understood. To obtain insight into the distinctiveness of juveniles from adults at the molecular level, we assessed long noncoding RNAs (lncRNAs) that are highly expressed selectively in juvenile cells. The noncoding elements of the transcriptome were investigated in hepatocytes and cardiomyocytes isolated from juvenile and adult mice. Here, we identified 62 Juvenility-associated lncRNAs (JAlncs), which are selectively expressed in both hepatocytes and cardiomyocytes from juvenile mice. Among these common (shared) JAlncs, Gm14230 is evolutionarily conserved and is essential for cellular juvenescence. Loss of Gm14230 impairs cell growth and causes cellular senescence. Gm14230 safeguards cellular juvenescence through recruiting the histone methyltransferase Ezh2 to Tgif2, thereby repressing the functional role of Tgif2 in cellular senescence. Thus, we identify Gm14230 as a Juvenility-selective lncRNA required to maintain cellular juvenescence.
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identification of Juvenility associated genes in the mouse hepatocytes and cardiomyocytes
Scientific Reports, 2018Co-Authors: Faidruz Azura Jam, Yosuke Kadota, Ikuo Tooyama, Masaki Mori, Anarmaa MendsaikhanAbstract:Young individuals possess distinct properties that adults do not. The juvenile animals show higher activities for growth, healing, learning and plasticity than adults. The machinery for establishing these juvenile properties is not fully understood. To better understand the molecular constituents for the above properties, we performed a comprehensive transcriptome analysis of differently aged cells of mice by high-throughput sequencing and identified the genes selectively highly expressed in the young cells. These genes, collectively called as Juvenility-associated genes (JAGs), show significant enrichments in the functions such as alternative splicing, phosphorylation and extracellular matrix (ECM). This implies the juvenescence might be achieved by these functions at the cell level. The JAG mutations are associated with progeria syndromes and growth disorders. Thus, the JAGs might organize the juvenile property of young animals and analysis of JAGs may provide scientific and therapeutic approaches toward treating the genetic diseases.
Melanie Pajon - One of the best experts on this subject based on the ideXlab platform.
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expression patterns of flowering genes in leaves of pineapple sweet orange citrus sinensis l osbeck and pummelo citrus grandis osb eck
BMC Plant Biology, 2017Co-Authors: Melanie Pajon, Vicente J Febres, Gloria A MooreAbstract:In citrus the transition from Juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of Juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and ‘Pineapple’ sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature ‘Pineapple’ seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions.
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Expression patterns of flowering genes in leaves of ‘Pineapple’ sweet orange [Citrus sinensis (L.) Osbeck] and pummelo (Citrus grandis Osbeck)
BMC, 2017Co-Authors: Melanie Pajon, Vicente J Febres, Gloria A MooreAbstract:Abstract Background In citrus the transition from Juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of Juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). Results In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and ‘Pineapple’ sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature ‘Pineapple’ seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. Conclusions In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions
Neena Mitter - One of the best experts on this subject based on the ideXlab platform.
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Juvenility and vegetative phase transition in tropical subtropical tree crops
Frontiers in Plant Science, 2019Co-Authors: Muhammad Umair Ahsan, Alice Hayward, Vered Irihimovitch, Stephen J Fletcher, Milos Tanurdzic, Alexander Pocock, Christine A Beveridge, Neena MitterAbstract:In plants, juvenile to adult phase transition is regulated by the sequential activity of two microRNAs: miR156 and miR172. A decline in miR156 and increase in miR172 abundance is associated with phase transition. There is very limited information on phase transition in economically important horticultural tree crops, which have a significantly long vegetative phase affecting fruit bearing. Here, we profiled various molecular cues known to be involved in phase transition and flowering, including the microRNAs miR156 and miR172, in three horticultural tree crops: avocado (Persea americana), mango (Mangifera indica), and macadamia (Macadamia integrifolia). We observed that miR156 expression decreases as these trees age and can potentially be used as a Juvenility marker. Consistent with findings in annual plants, we also observed conserved regulation of the miR156-SPL3/4/5 regulatory module in these genetically distant tree crops, suggesting that this pathway may play a highly conserved role in vegetative identity. Meanwhile, the abundance of miR172 and its target AP2-like genes as well as the accumulation level of SPL9 transcripts were not related with plant age in these crops except in avocado where miR172 expression increased steadily. Finally, we demonstrate that various floral genes, including AP1 and SOC1 were upregulated in the reproductive phase and can be used as potential markers for the reproductive phase transition. Overall, this study provides an insight into the molecular associations of Juvenility and phase transition in horticultural trees where crop breeding and improvement are encumbered by long juvenile phases.
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temporal profile of Juvenility associated micrornas during tissue culture of avocado
Acta Horticulturae, 2018Co-Authors: J Hiti C A Bandaralage, Alice Hayward, Christopher J Obrien, Neena MitterAbstract:MicroRNAs are a group of non-coding small RNAs of 21-22 nucleotides found in many multicellular organisms including both animals and plants. They are highly conserved across many plant species and play a key role in regulating plant development by either directing cleavage of complimentary mRNAs or by translational inhibition. Avocado (Persea americana Mill.) is a high value tropical fruit crop which could be benefited through tissue culture to improve propagation of elite lines. Many morphological, physiological, biochemical and molecular changes occur during the in vitro tissue culture process. It has been hypothesised that the tissue culture process restores juvenile characteristics to explants obtained from physiologically mature mother plants. This hypothesis was examined in the current study by profiling expression levels of two highly conserved microRNAs (miR156 and miR172) involved in juvenile-to-adult phase change. The preliminary results reveal that miR172, a marker for plant maturity, is significantly reduced during the in vitro culture process. This is the first molecular indication of physiologically mature avocado material regaining Juvenility characteristics as a result of the tissue culture process.
Wen-wu Guo - One of the best experts on this subject based on the ideXlab platform.
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Somatic cybrid production via protoplast fusion for citrus improvement
Scientia Horticulturae, 2013Co-Authors: Wen-wu Guo, Shi-xin Xiao, Xiuxin DengAbstract:Abstract Somatic hybridization holds great potential in citrus improvement by circumventing several biological barriers such as nucellar polyembryony, long Juvenility and pollen/ovule sterility. To date more than 300 intergeneric and interspecific citrus somatic hybrids have been produced by standard symmetric fusion; among which, more than forty parental combinations produced leaf-parent-type cybrids or putative cybrids. Herein somatic cybrid production by protoplast fusion aiming to produce citrus cybrids with male sterile cytoplasm being transferred simply via symmetric fusion is detailed including methods to produce cybrids, possible mechanism and factors involved in cybrid production via symmetric fusion, strategy of targeted cybrid production for potential seedlessness, and agronomic performance and omics-based studies of some selected cybrids. This review provides the most comprehensive and updated information concerning citrus cybrid production over the past 20 years.
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highly efficient transformation of the gfp and mac12 2 genes into precocious trifoliate orange poncirus trifoliata l raf a potential model genotype for functional genomics studies in citrus
Tree Genetics & Genomes, 2009Co-Authors: Bin Tan, Gaien Fan, Jing Fan, Wen-wu GuoAbstract:Precocious trifoliate orange (Poncirus trifoliata [L.] Raf), an extremely early flowering mutant of P. trifoliata, is an attractive model for functional genomics research in Citrus. A procedure for efficient regeneration and transformation of this genotype was developed by using green fluorescent protein (GFP) gene as visual marker and etiolated stem segments as explants. In vivo monitoring of GFP expression permitted a rapid and easy discrimination of transgenic shoots and escapes. Transformation efficiency was 20.7% and the transformants were identified by polymerase chain reaction (PCR) and Southern blot analysis. Moreover, the transgenic lines expressed variable amounts of the GFP gene as revealed by real-time PCR analysis. Fifteen transgenic plants flowered 18 months after transfer to the greenhouse and six of them set fruits. GFP expression was also observed in the transgenic flowers and fruits. To test the utility of this system for functional genomics studies, an Arabidopsis thaliana MAC12.2 gene with the potential to produce seedless fruits was introduced into this genotype, and the traits of the transgenic fruits were characterized. The successful transformation of this perennial woody genotype with extremely short Juvenility will allow us to test the function of cloned genes in citrus, the improvement of which is hindered by a long Juvenility period.
