The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Margrethe Serek - One of the best experts on this subject based on the ideXlab platform.
-
USE OF THE PMI/MANNOSE SELECTION SYSTEM FOR GENETIC TRANSFORMATION OF Kalanchoe blossfeldiana POELLN.
2015Co-Authors: Agnieszka Ilczuk, Heiko Mibus, Malthe Schmidt, Margrethe SerekAbstract:AidA R., ShibAtA M. (1996). Transformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgen
-
manipulation of mks1 gene expression affects Kalanchoe blossfeldiana and petunia hybrida phenotypes
Plant Biotechnology Journal, 2015Co-Authors: J. M. Gargul, Heiko Mibus, Margrethe SerekAbstract:ummary The establishment of alternative methods to chemical treatments for growth retardation and pathogen protection in ornamental plant production has become a major goal in recent breeding programmes. This study evaluates the effect of manipulating MAP kinase 4 nuclear substrate 1 (MKS1) expression in Kalanchoe blossfeldiana and Petunia hybrida. The Arabidopsis thaliana MKS1 gene was overexpressed in both species via Agrobacterium-mediated transformation, resulting in dwarfed phenotypes and delayed flowering in both species and increased tolerance to Pseudomonas syringae pv. tomato in transgenic Petunia plants. The lengths of the stems and internodes were decreased, while the number of nodes in the transgenic plants was similar to that of the control plants in both species. The transgenic Kalanchoe flowers had an increased anthocyanin concentration, and the length of the inflorescence stem was decreased. The morphology of transgenic Petunia flowers was not altered. The results of the Pseudomonas syringae tolerance test showed that Petunia plants with one copy of the transgene reacted similarly to the nontransgenic control plants; however, plants with four copies of the transgene exhibited considerably higher tolerance to bacterial attack. Transgene integration and expression was determined by Southern blot hybridization and RT-PCR analyses. MKS1 in wild-type Petunia plants was down-regulated through a virus-induced gene silencing (VIGS) method using tobacco rattle virus vectors. There were no significant phenotypic differences between the plants with silenced MKS1 genes and the controls. The relative concentration of the MKS1 transcript in VIGS-treated plants was estimated by quantitative RT-PCR.
-
constitutive overexpression of nicotiana ga 2 ox leads to compact phenotypes and delayed flowering in Kalanchoe blossfeldiana and petunia hybrida
Plant Cell Tissue and Organ Culture, 2013Co-Authors: J. M. Gargul, Heiko Mibus, Margrethe SerekAbstract:This work describes compact phenotypes of Kalanchoe blossfeldiana and Petunia hybrida plants harboring a constitutively overexpressed gibberellin 2-oxidase (GA 2 ox) transgene. A GA 2 ox gene from Nicotiana tabacum under the control of the Ca35S promoter was introduced into the pCAMBIA1303 plasmid. The cloning vector was introduced into leaf explants of Kalanchoe and Petunia via Agrobacterium-mediated transformation. Putative transformants were analysed for the presence, integration and expression of the transgene using polymerase chain reaction (PCR), reverse-transcription (RT)-PCR, and Southern blot analysis, respectively. Phenotypic evaluations revealed that the mean lengths of the Kalanchoe transgenic lines were two-fold shorter than those of wild-type control plants, although the mean numbers of nodes were similar. Moreover, the mean lengths of inflorescence stems of the Kalanchoe transgenic lines were almost three-fold shorter than those of the wild-type control plants. Similarly, the mean lengths of Petunia transgenic lines were four-fold shorter than those of the wild-type plants, except for a single line, while the mean numbers of nodes were either similar or higher in the transgenic lines than in the wild-type control plants. In transgenic lines of both Kalanchoe and Petunia, delayed flowering was observed with a mean of 24 days for Kalanchoe and a range of three to 12 days for Petunia. Although the flower morphology of the transgenic lines did not exhibit any differences from their respective wild-type control plants, transgenic lines of both species exhibited darker green pigmented leaves containing an approximately two-fold increase in chlorophyll contents over the wild-type control plants.
-
use of the pmi mannose selection system for genetic transformation of Kalanchoe blossfeldiana poelln
Propagation of Ornamental Plants, 2009Co-Authors: Agnieszka Ilczuk, Heiko Mibus, Malthe Schmidt, Margrethe SerekAbstract:Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana ‘Molly’ and breeding line ‘1998-469’. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l -1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l -1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for ‘Molly’ and 6.7-39% for breeding line ‘1998-469’. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
-
transformation of Kalanchoe blossfeldiana with rol genes is useful in molecular breeding towards compact growth
Plant Cell Reports, 2008Co-Authors: Brian Christensen, Margrethe Serek, Sridevy Sriskandarajah, Renate MullerAbstract:Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding.
Renate Muller - One of the best experts on this subject based on the ideXlab platform.
-
stigma development and receptivity of two Kalanchoe blossfeldiana cultivars
Acta Physiologiae Plantarum, 2014Co-Authors: Leila Therese Traore, Henrik Lutken, Katarzyna Kuligowska, Renate MullerAbstract:Several members of the Kalanchoe genus are popular as ornamental plants. Cross-breeding and wide hybridisation are essential to continuously introduce novel traits into cultivated plant material. This study aimed to identify the major factors related to the stigma affecting cross-pollination in the Kalanchoe blossfeldiana. Pollen tube growth after pollination of K. blossfeldiana ‘Jackie’ and ‘Reese’ was examined at different stigma developmental stages. Five distinct developmental stages were identified based on changes in morphology and activity of stigmatic peroxidase. After reciprocal pollination at the five stigma developmental stages, fluorescence microscopy was used to estimate the number of pollen tubes in situ. Both cultivars had receptive stigmas from stage I to IV, which concurred with the continuous expansion of the stigma covered with exudates. No pollen tube growth was observed at stage V for both cultivars. The number of pollen tubes was significantly higher in carpels pollinated at stage III, characterized by loose arrangement of the papillae and maximal amount of exudates, compared to all other developmental stages. Stigmas showing drying exudates and absence of peroxidase exhibited a relatively decreased number of pollen tubes in situ. No pollen tubes germinated on wilting stigmas. The arrangement of the papillae, the presence of exudates and peroxidase activity affected the number of pollen tubes in cross-pollination of K. blossfeldiana cultivars ‘Jackie’ and ‘Reese’. These results will help breeders to better select the optimal time for effective pollination. The findings may be applicable for other cultivars of K. blossfeldiana and relevant for different species of Kalanchoe.
-
biomass distribution in Kalanchoe blossfeldiana transformed with rol genes of agrobacterium rhizogenes
Hortscience, 2009Co-Authors: Brian Christensen, Sridevy Sriskandarajah, Renate MullerAbstract:Kalanchoe blossfeldiana transformed with Agrobacterium rhizogenes exhibited marked alterations in morphology and biomass distribution. Plants termed root- inducing (Ri) lines were regenerated from hairy roots produced by inoculating leaf explants with Agrobacterium rhizogenes wild-type strain ATCC15834. Six Ri lines were characterized in a greenhouse trial and all Ri lines had reduced dry weights of main shoot, lateral shoots, leaves, and flowers compared with control plants. The reduction in dry weights of these organs correlated with reduced plant height, shoot length, leaf area, and number of flowers per plant. Furthermore, an altered distribution of dry matter was evident in the Ri plants, where the greater part of dry matter was allocated into leaves and secondly into flowers, whereas the majority of dry matter in control plants was allocated into flowers and secondly into leaves. Furthermore, a higher percentage of dry matter was allocated into the main shoot of the Ri lines in comparison with that of control plants. Increased dry matter in leaves and in the main shoot in the Ri lines appeared to be at the expense of dry matter allocated into flowers. Moreover, an increased number of vegetative lateral shoots was recorded in the Ri lines, whereas the number of reproductive lateral shoots was decreased. Possible mechanisms behind the altered resource distribution are discussed.
-
Kalanchoe blossfeldiana transformed with rol genes exhibits improved postharvest performance and increased ethylene tolerance
Postharvest Biology and Technology, 2009Co-Authors: Brian Christensen, Renate MullerAbstract:Abstract Kalanchoe blossfeldiana transformed with rol genes, termed root-inducing (Ri) lines, which had been produced through transformation using Agrobacterium rhizogenes wild-type strain ATCC15834, were tested for their postharvest performance. Flower longevity, ethylene sensitivity and display quality varied among control and chemical growth retarded plants and the Ri lines. Both chemical growth retardation and transformation with rol genes reduced the number of flowers in comparison to control plants. Detached single flowers of the two Ri lines tested, Ri line 306 and 331, had a longevity, defined as the stage where the flowers were 70% open, of 32 and 34 d, respectively, whereas chemical growth retarded and control plants had a longevity of 28 and 26 d, respectively. Ri line 331 had the significantly longest display life of 34 d to reach the stage of losing the ornamental value of the whole plant, defined as 10% wilted flowers. The corresponding display life of control and chemical retarded plants and plants of Ri line 306 was 25 d. The opening of the flowers was more synchronous in the Ri lines than control plants. Exposed to ethylene, the flowers of the Ri lines exhibited reduced responsiveness whereas chemical growth retarded and control plants were sensitive. Possible mechanisms behind the improved postharvest performance of plants transformed with rol genes are discussed.
-
transformation of Kalanchoe blossfeldiana with rol genes is useful in molecular breeding towards compact growth
Plant Cell Reports, 2008Co-Authors: Brian Christensen, Margrethe Serek, Sridevy Sriskandarajah, Renate MullerAbstract:Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding.
J Brulfert - One of the best experts on this subject based on the ideXlab platform.
-
identification of multiple pepc isogenes in leaves of the facultative crassulacean acid metabolism cam plant Kalanchoe blossfeldiana poelln cv tom thumb
FEBS Letters, 1995Co-Authors: H Gehrig, Manfred Kluge, Tahar Taybi, J BrulfertAbstract:In the facuitative Crassulacean Acid Metabolism (CAM) plant Kalanchoe blossfeldiana cv. Tom Thumb, CAM can be induced by short-day treatment or water deficiency stress. From young leaves of well-watered and water-stressed individuals of this plant, cDNA clones coding for a partial sequence of the key enzyme of CAM, phnsphoenolpyruvate carboxylase, were isolated after transcription of mRNA. cDNA polymorphism was established by enzyme restriction profiles and sequencing data. Four PEPC isogenes could be shown to exist inK. blossfeidiana forming two geue pairs, with 95%-98% homology inside and only 75% between the pairs. One cDNA sequence pair having a length of 1113 bp and an open reading frame of 371 AA was identified as PEPC isoform specific for the C3 state, whereas the pair having a length of 1116 bp and an open reading frame of 372 AA could be attributed to the CAM state. These results were con- firmed by Southern Blot hybridization. (EMBL, accession num- bers X 87818, X 87819, X 87820, X 87821.)
-
differential effects of abscisic acid on phosphoenolpyruvate carboxylase and cam operation in Kalanchoe blossfeldiana
Plant Biology, 1995Co-Authors: Tahar Taybi, S Guclu, Manfred Kluge, B Sotta, H Gehrig, J BrulfertAbstract:Photoperiodism or water stress can shift young leaves of Kalanchoe blossfeldiana from C3-type photosynthesis to Crassulacean acid metabolism (CAM). CAM induction implies synthesis of phosphoenolpyruvate carboxylase (EC 4.1.1.31, PEPC). Present results show that, in plants as well as in isolated leaves, an increase in endogenous levels of abscisic acid (ABA) preceded PEPC increase, independently of the nature of the CAM inductive parameter. Exogenously applied ABA was taken up and accumulated by isolated leaves to a level similar to the internal ABA concentration in the leaf tissues. Both in entire plants and in isolated leaves, exogenous ABA mimicked the effects of water stress or short days in triggering PEPC capacity. Using a heterologous cDNA probe (derived from Sorghum leaf PEPC gene) it could be shown that an early step of the leaf response to drought or ABA treatment consisted in an increase in the amount of PEPC transcripts. Depending on the duration of ABA application, C3 photosynthesis, typical CAM or CAM-idling could be triggered in isolated leaves. In each case, similar increases in PEPC capacity (maximum extractable activity) were obtained, suggesting that the type of photosynthesis performed by the leaves is not controlled by the level of the PEPC capacity. The results suggest that the photosynthetic behaviour of the leaves is primarily governed by the progressive drop in availability of external carbon caused by the ABA-controlled closure of the stomata. Effects of ABA on PEPC synthesis proceed independently of CAM performance.
-
enzymatic responses to water stress in detached leaves of the cam plant Kalanchoe blossfeldiana poelln
Plant Physiology and Biochemistry, 1993Co-Authors: J Brulfert, S Guclu, Tahar Taybi, J N PierreAbstract:Total extractable activity («capacity») of phosphoenolpyruvate carboxylase (EC 4.1.1.31, PEPC), phosphofructokinase (EC 2.7.1.1, PKF), aldolase (EC 4.1.2.7), phosphoglycerate mutase (EC 5.4.2.1, PGM), enolase (EC 4.2.1.11), glucose-6-phosphate dehydrogenase (EC 1.1.1.49, G-6-PDH) and phenylalanine ammonia lyase (EC 4.3.1.5, PAL) was measured in leaves detached from the CAM plant Kalanchoe blossfeldiana grown under long days (C 3 -performing). The isolated leaves were maintained 36 h under 280 μmol m −2 s −1 PAR, at 27°C, under drought (50% relative air humidity)
Heiko Mibus - One of the best experts on this subject based on the ideXlab platform.
-
USE OF THE PMI/MANNOSE SELECTION SYSTEM FOR GENETIC TRANSFORMATION OF Kalanchoe blossfeldiana POELLN.
2015Co-Authors: Agnieszka Ilczuk, Heiko Mibus, Malthe Schmidt, Margrethe SerekAbstract:AidA R., ShibAtA M. (1996). Transformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgen
-
manipulation of mks1 gene expression affects Kalanchoe blossfeldiana and petunia hybrida phenotypes
Plant Biotechnology Journal, 2015Co-Authors: J. M. Gargul, Heiko Mibus, Margrethe SerekAbstract:ummary The establishment of alternative methods to chemical treatments for growth retardation and pathogen protection in ornamental plant production has become a major goal in recent breeding programmes. This study evaluates the effect of manipulating MAP kinase 4 nuclear substrate 1 (MKS1) expression in Kalanchoe blossfeldiana and Petunia hybrida. The Arabidopsis thaliana MKS1 gene was overexpressed in both species via Agrobacterium-mediated transformation, resulting in dwarfed phenotypes and delayed flowering in both species and increased tolerance to Pseudomonas syringae pv. tomato in transgenic Petunia plants. The lengths of the stems and internodes were decreased, while the number of nodes in the transgenic plants was similar to that of the control plants in both species. The transgenic Kalanchoe flowers had an increased anthocyanin concentration, and the length of the inflorescence stem was decreased. The morphology of transgenic Petunia flowers was not altered. The results of the Pseudomonas syringae tolerance test showed that Petunia plants with one copy of the transgene reacted similarly to the nontransgenic control plants; however, plants with four copies of the transgene exhibited considerably higher tolerance to bacterial attack. Transgene integration and expression was determined by Southern blot hybridization and RT-PCR analyses. MKS1 in wild-type Petunia plants was down-regulated through a virus-induced gene silencing (VIGS) method using tobacco rattle virus vectors. There were no significant phenotypic differences between the plants with silenced MKS1 genes and the controls. The relative concentration of the MKS1 transcript in VIGS-treated plants was estimated by quantitative RT-PCR.
-
constitutive overexpression of nicotiana ga 2 ox leads to compact phenotypes and delayed flowering in Kalanchoe blossfeldiana and petunia hybrida
Plant Cell Tissue and Organ Culture, 2013Co-Authors: J. M. Gargul, Heiko Mibus, Margrethe SerekAbstract:This work describes compact phenotypes of Kalanchoe blossfeldiana and Petunia hybrida plants harboring a constitutively overexpressed gibberellin 2-oxidase (GA 2 ox) transgene. A GA 2 ox gene from Nicotiana tabacum under the control of the Ca35S promoter was introduced into the pCAMBIA1303 plasmid. The cloning vector was introduced into leaf explants of Kalanchoe and Petunia via Agrobacterium-mediated transformation. Putative transformants were analysed for the presence, integration and expression of the transgene using polymerase chain reaction (PCR), reverse-transcription (RT)-PCR, and Southern blot analysis, respectively. Phenotypic evaluations revealed that the mean lengths of the Kalanchoe transgenic lines were two-fold shorter than those of wild-type control plants, although the mean numbers of nodes were similar. Moreover, the mean lengths of inflorescence stems of the Kalanchoe transgenic lines were almost three-fold shorter than those of the wild-type control plants. Similarly, the mean lengths of Petunia transgenic lines were four-fold shorter than those of the wild-type plants, except for a single line, while the mean numbers of nodes were either similar or higher in the transgenic lines than in the wild-type control plants. In transgenic lines of both Kalanchoe and Petunia, delayed flowering was observed with a mean of 24 days for Kalanchoe and a range of three to 12 days for Petunia. Although the flower morphology of the transgenic lines did not exhibit any differences from their respective wild-type control plants, transgenic lines of both species exhibited darker green pigmented leaves containing an approximately two-fold increase in chlorophyll contents over the wild-type control plants.
-
a search for growth related genes in Kalanchoe blossfeldiana
Plant Physiology and Biochemistry, 2009Co-Authors: Sine Hovbye Topp, Soren K Rasmussen, Heiko Mibus, Lilli SanderAbstract:Abstract Differential display of mRNA from four sets of contrasting phenotypes were carried out in order to identify and isolate genes associated with elongating growth of Kalanchoe blossfeldiana . A total of 17 unique differential expressed cDNA fragments were sequenced and 12 showed homology to genes in other plant species. Three genes were subsequently tested for growth related activity by Virus Induced Gene Silencing (VIGS) in Nicotiana benthamiana . One gene fragment (13C) resulted in plants with significantly reduced growth ( N = 20, P = 0.05, one-tailed students t -test) from day 25 after virus infection. Full-length cDNA and genomic DNA sequences were obtained by inverse PCR and thermal asymmetric interlaced (TAIL) PCR and the gene was named KbORF1 . The predicted gene is 2244 bp long with three exons of 411 bp in total encoding a protein of 137 amino acid residues with homologs widespread among plants. The protein has no known function, but its expression has been confirmed in a proteomic study of Arabidopsis . Southern blot analysis shows two hybridizing fragments in agreement with the tetraploid nature of K. blossfeldiana . Fragment 13C comprises 446 bp of the gene, and the portion of 13C conferring growth retardation by VIGS is located 10 bp into the second intron indicating a regulatory function of this part of the KbORF1 mRNA. Differential display in combination with VIGS as a screening method proved to be a good functional approach not only to search for genes of interest, but also to isolate expressed genetic regulatory domains.
-
use of the pmi mannose selection system for genetic transformation of Kalanchoe blossfeldiana poelln
Propagation of Ornamental Plants, 2009Co-Authors: Agnieszka Ilczuk, Heiko Mibus, Malthe Schmidt, Margrethe SerekAbstract:Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana ‘Molly’ and breeding line ‘1998-469’. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l -1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l -1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for ‘Molly’ and 6.7-39% for breeding line ‘1998-469’. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
Ana Rita Costenaro Parizi - One of the best experts on this subject based on the ideXlab platform.
-
efeito da dotacao de rega em cultivares de Kalanchoe blossfeldiana
Revista de Ciências Agrárias, 2015Co-Authors: Fatima Cibele Soares, Ana Rita Costenaro Parizi, Francieli Ribeiro Correa, Francielle Altissimo Bortolas, Glaucia Pinheiro, Veronica Pereira Da Rosa, Jumar Luis RussiAbstract:Com o crescente aumento de consumo de flores e plantas ornamentais de alta qualidade e, a disseminacao das tecnicas de cultivo no meio do agronegocio, aliado a escassez de recursos hidricos, torna-se imprescindivel o conhecimento do manejo adequado da agua de irrigacao, na floricultura. Dentro deste contexto, o presente trabalho tem por objetivo avaliar o efeito de niveis de irrigacao sobre cultivares de Kalanchoe blossfeldiana Poelln em ambiente protegido. O experimento foi conduzido em estufa. O delineamento experimental foi fatorial 4x3, sendo quatro lâminas de irrigacao (100, 80, 60 e 40% da capacidade de vaso - CV) e tres cultivares (Debbie, Gold Jewel e Simone). Foram avaliados, a altura de planta, numero de folhas por planta e area do dossel vegetativo. No final do ciclo, aos 252 dias apos transplante foi determinado o numero de flores por planta e calculado o consumo diario de agua. Determinou-se a eficiencia do uso da agua, pelo quociente obtido entre a produtividade da cultura (em numero de flores por vaso) e a lâmina total de agua aplicada durante o ciclo da cultura, em mm. As variaveis foram submetidas a analise da variância a 5%. Observou-se interacao significativa, apenas, entre os fatores, para a area do dossel, com destaque para a cultivar Gold Jewel na lâmina de 40%. As cultivares Debbie e Simone apresentaram a maior eficiencia no uso da agua, diferindo estatisticamente da variedade Gold Jewel. Conclui-se que a lâmina com 40% da CV e suficiente para atender a demanda hidrica das cultivares.
-
efeito da dotacao de rega em cultivares de Kalanchoe blossfeldiana effect of irrigation on cultivars of Kalanchoe blossfeldiana
2015Co-Authors: Fatima Cibele Soares, Ana Rita Costenaro Parizi, Francieli Ribeiro Correa, Francielle Altissimo Bortolas, Jumar Luis RussiAbstract:With the growing consumer demand for high quality flowers and ornamental plants, and the spread of farming techni ques in the agribusiness, combined with scarce water resources, it is imperative to stablish the proper water irrigation management for floriculture. In this context, this work aims to evaluate the effect of different irrigation levels on varie ties of Kalanchoe blossfeldiana Poelln in a protected environment. The experiment was conducted in a greenhouse. The experimental design was factorial 4x3, with four irrigation levels (100, 80, 60 and 40% of pot capacity - CV) and three cultivars (‘Debbie’, ‘Gold Jewel’ and ‘Simone’). Throughout the plant growth cycle the plant height, the number of leaves per plant and the canopy area, for each treatment were evaluated weekly. At the end of the cycle, by 252 days after transplanting, was determined the number of flowers per plant and calculated the daily water consumption. The water use efficiency was determined by the ratio between the number of flowers per pot, and the total irrigation water applied during the growth cycle, in mm. The variables were subjected to an analysis of variance at 5%. A significant interaction between the factors was observed only for the area of the canopy, with emphasis for the ‘Gold Jewel’ variety with 40% level of irrigation. The ‘Debbie’ and ‘Simone’ varieties showed greater efficiency in water use, differing statistically from the ‘Gold Jewel’ variety. It is concluded that the 40% of CV level is enough meet the water demand of crops.
-
modelo para determincao da area foliar de Kalanchoe blossfeldiana poelln
Ciencia Rural, 2006Co-Authors: Marcia Xavier Peiter, Adroaldo Dias Robaina, Ana Rita Costenaro PariziAbstract:This research was aimed at versifying a mathematical procedure that allows the description of leaf of Kalanchoe (Kalanchoe blossfeldiana Poelln.) and the estimation of its photosynthetically active leaf area starting from a non destructive leaf determination. Seedlings of Kalanchoe cv Gold Jewel were cultivated in irrigated vases with recommended doses for the culture. Three vases were removed weekly from the greenhouse and the plants had their leaves cut, identified and submitted to measures in plant position, longitudinal and transverse length. A total of nine data collection was carried out weekly since 04/04/2003 until blossom. Three plants were sampled weekly and leaf area were calculated using Gauss method (GARCIA & PIEDADE, 1944) implemented in Visual Basic specifically for this purpose. A total of 979 leaves were sampled and the verification of the possibility of using a medium correction factor (FCM) for leaf area independently of its position and growth cycle in the plant, was found out through regression analysis among the values obtained by the standard method (Gauss) and the estimated values for FCM method. The experimental results indicate that the value FCM=1.1134 can be used to estimate leaf area by multiplying length and width leaf values in any phase of the plant and any position of the leaf in the plant.
-
Modelo para determinção da área foliar de Kalanchoe blossfeldiana Poelln Model for leaf area determination in Kalanchoe blossfeldiana Poelln
Universidade Federal de Santa Maria, 2006Co-Authors: Marcia Xavier Peiter, Adroaldo Dias Robaina, Ana Rita Costenaro PariziAbstract:O presente trabalho teve por objetivo a verificação de um procedimento matemático que permita a descrição do crescimento foliar de Kalanchoe (Kalanchoe blossfeldiana Poelln.) e possa prever a sua área foliar fotossinteticamente ativa a partir de medidas não destrutivas de folhas. As mudas de Kalanchoe Cv. "Gold Jewel" foram cultivadas para o procedimento experimental em vasos irrigados com doses recomendadas para a cultura. Semanalmente, foram retirados três vasos da estufa e as plantas tiveram suas folhas cortadas, identificadas e submetidas a tomadas de medidas de sua posição na planta, do máximo comprimento longitudinal e do máximo comprimento transversal. Foram realizadas um total de nove coletas semanalmente, desde 04/04/2003 até o início da floração. Em cada coleta, três plantas eram amostradas e a área foliar calculada com a utilização do método de Gauss (GARCIA & PIEDADE, 1944) implementado em Visual Basic especificamente para este objetivo. Foram amostradas um total de 979 folhas e a verificação da possibilidade de uso de um fator de correção médio (FCM) para o cálculo da área de uma folha, independentemente de sua posição na planta ou fase do ciclo de crescimento, foi averiguada por análise de regressão entre os valores obtidos pelo método padrão (Gauss) e os valores estimados pelo método do FCM. Os resultados experimentais indicam que o valor FCM=1,1134 pode ser utilizado para estimar a área foliar pela multiplicação pelos valores de comprimento e largura de folha em qualquer fase do cultivo e sem qualquer posição da folha na planta.This research was aimed at versifying a mathematical procedure that allows the description of leaf of Kalanchoe (Kalanchoe blossfeldiana Poelln.) and the estimation of its photosynthetically active leaf area starting from a non destructive leaf determination. Seedlings of Kalanchoe cv Gold Jewel were cultivated in irrigated vases with recommended doses for the culture. Three vases were removed weekly from the greenhouse and the plants had their leaves cut, identified and submitted to measures in plant position, longitudinal and transverse length. A total of nine data collection was carried out weekly since 04/04/2003 until blossom. Three plants were sampled weekly and leaf area were calculated using Gauss method (GARCIA & PIEDADE, 1944) implemented in Visual Basic specifically for this purpose. A total of 979 leaves were sampled and the verification of the possibility of using a medium correction factor (FCM) for leaf area independently of its position and growth cycle in the plant, was found out through regression analysis among the values obtained by the standard method (Gauss) and the estimated values for FCM method. The experimental results indicate that the value FCM=1.1134 can be used to estimate leaf area by multiplying length and width leaf values in any phase of the plant and any position of the leaf in the plant
