The Experts below are selected from a list of 177 Experts worldwide ranked by ideXlab platform
Katsutoshi Terasawa - One of the best experts on this subject based on the ideXlab platform.
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Oren-gedoku-to and Keishi-Bukuryo-Gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits.
Biological & pharmaceutical bulletin, 2005Co-Authors: Nobuyasu Sekiya, Yutaka Shimada, Takako Nakagawa, Hiroaki Hikiami, Naotoshi Shibahara, Mosaburo Kainuma, Kazufumi Kouta, Katsutoshi TerasawaAbstract:In this study, we examined whether the Kampo formulas Oren-gedoku-to (OGT, Huanglian-jie-du-tang in Chinese) and Keishi-Bukuryo-Gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) could prevent the progression of atherosclerosis in cholesterol-fed rabbit, an animal model for hypercholesterolemia in vivo. Twenty-four male Japanese white rabbits (2 kg body weight) were divided into four groups. The control group was fed standard rabbit chow containing 1% cholesterol, the OGT group was fed standard rabbit chow containing 1% cholesterol and 1% OGT, the KBG group was fed standard rabbit chow containing 1% cholesterol and 1% KBG, and the vitamin E group was fed standard rabbit chow containing 1% cholesterol and vitamin E (450 mg/1000 g). All four groups were kept on these diets for 8 weeks. At the end of the experiments, the percentage of surface area of the total thoracic aorta with visible plaque was significantly reduced in the OGT and KBG groups. The serum thiobarbituric acid reactive substances of the vitamin E group showed a significantly low value compared with the control group, whereas the serum lipid peroxide levels of the OGT and KBG groups were considerably lower than that of the control groups as well as that of the vitamin E group. Furthermore, the urinary 8-hydroxydeoxyguanosine levels of the OGT and KBG groups were considerably lower than that of the vitamin E group. These results suggest that OGT and KBG prevent the progression of atheromatous plaque by creating a sounder antioxidant defense system than vitamin E.
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Effects of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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effects of Keishi Bukuryo Gan on vascular function and hemorheological factors in spontaneously diabetic wbn kob rats
Phytomedicine, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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Protective effect of Keishi-Bukuryo-Gan and its constituent medicinal plants against nitric oxide donor-induced neuronal death in cultured cerebellar granule cells.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Yutaka Shimada, Nobuyasu Sekiya, Hirozo Goto, Naoki Mantani, Hiroaki Hikiami, Koichi Yokoyama, Eiichi Tahara, Katsutoshi TerasawaAbstract:Abstract Keishi-Bukuryo-Gan (Gui-Zhi-Fu-Ling-Wan) (KBG) is a traditional Chinese/Japanese medical (Kampo) formulation that has been administered to patients with “Oketsu” (blood stagnation) syndrome. In the process of neuronal cell death induced by brain ischemia, excessive generation of nitric oxide (NO) free radicals is implicated in the neurotoxicity. In the present study, we examined the protective effects of KBG and its constituent medicinal plants against NO donors, sodium nitroprusside (SNP) and 2,2′-(hydroxynitrosohydrazino) bis -ethanamine (NOC18)-induced neuronal death in cultured rat cerebellar granule cells (CGCs). MTT assay showed cell viability to be significantly increased by the addition of KBG extract (KBGE) (100 μg/ml), Cinnamomi Cortex extract (CCE) (3, 10 and 30 μg/ml), Paeoniae Radix extract (PRE) (100 μg/ml) and Moutan Cortex extract (MCE) (10 and 30 μg/ml) compared with exposure to SNP (30 μM, 24 h) only. Also, cell viability was significantly increased by the addition of KBGE (100 and 300 μg/ml), CCE (30 and 100 μg/ml), PRE (100 and 300 μg/ml) and MCE (30 and 100 μg/ml) compared with exposure to NOC18 (100 μM, 48 h) only. Persicae Semen extract and Hoelen extract did not protect against NO donor-induced neuronal death. These results suggest that KBG has protective effect against NO-mediated neuronal death in cultured CGCs and that it is derived from Cinnamomi Cortex, Paeoniae Radix and Moutan Cortex.
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effect of Keishi Bukuryo Gan on autonomic nervous activity
和漢医薬学雑誌 = Journal of traditional medicines, 2003Co-Authors: 直利 柴原, Nobuyasu Sekiya, Yutaka Shimada, Hirozo Goto, Naoki Mantani, Eiichi Tahara, Katsutoshi TerasawaAbstract:In order to confirm any effects ofKeishi－Bukuryo－Gan（KBG）on the autonomic nervous system，8healthy volun－ teers were evaluated with laser Doppler flowmetry and spectral analysis ofthe R－R intervals（RR）and systolic blood pressure（SBP）．After evaluation ofthe窟’oketsu”score and measurement ofthe electrophysiological parameters（PRE）， each subject was administere（1KBG or hot water。The same parameters were re－measured at15，30，45，60and90min－ utes after their administration to each subject．Changes in the parameters after the administration of KBG or hot water were investigated compared with PRE．In the experiment with KBG，skin blood flow（SBF）was increased at 90min，although it had been significantly decreased at15min。By spectral analysis，SBP－10w frequency（LF）and SBP．10w to high frequency ratio（L／H）were initially increased at15min，and then were significantly decreased at 60and90min．As for hot water，there were no significant changes in any of the parameters．It is known that SBF changes with sympathetic nervous activity，and SBP－LF and SBP－L／H reflect sympathetic nervous activity．These re－ sults suggest that KBG has certain effects on sympathetic nervous activity．
Hirozo Goto - One of the best experts on this subject based on the ideXlab platform.
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Effects of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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effects of Keishi Bukuryo Gan on vascular function and hemorheological factors in spontaneously diabetic wbn kob rats
Phytomedicine, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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Protective effect of Keishi-Bukuryo-Gan and its constituent medicinal plants against nitric oxide donor-induced neuronal death in cultured cerebellar granule cells.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Yutaka Shimada, Nobuyasu Sekiya, Hirozo Goto, Naoki Mantani, Hiroaki Hikiami, Koichi Yokoyama, Eiichi Tahara, Katsutoshi TerasawaAbstract:Abstract Keishi-Bukuryo-Gan (Gui-Zhi-Fu-Ling-Wan) (KBG) is a traditional Chinese/Japanese medical (Kampo) formulation that has been administered to patients with “Oketsu” (blood stagnation) syndrome. In the process of neuronal cell death induced by brain ischemia, excessive generation of nitric oxide (NO) free radicals is implicated in the neurotoxicity. In the present study, we examined the protective effects of KBG and its constituent medicinal plants against NO donors, sodium nitroprusside (SNP) and 2,2′-(hydroxynitrosohydrazino) bis -ethanamine (NOC18)-induced neuronal death in cultured rat cerebellar granule cells (CGCs). MTT assay showed cell viability to be significantly increased by the addition of KBG extract (KBGE) (100 μg/ml), Cinnamomi Cortex extract (CCE) (3, 10 and 30 μg/ml), Paeoniae Radix extract (PRE) (100 μg/ml) and Moutan Cortex extract (MCE) (10 and 30 μg/ml) compared with exposure to SNP (30 μM, 24 h) only. Also, cell viability was significantly increased by the addition of KBGE (100 and 300 μg/ml), CCE (30 and 100 μg/ml), PRE (100 and 300 μg/ml) and MCE (30 and 100 μg/ml) compared with exposure to NOC18 (100 μM, 48 h) only. Persicae Semen extract and Hoelen extract did not protect against NO donor-induced neuronal death. These results suggest that KBG has protective effect against NO-mediated neuronal death in cultured CGCs and that it is derived from Cinnamomi Cortex, Paeoniae Radix and Moutan Cortex.
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effect of Keishi Bukuryo Gan on autonomic nervous activity
和漢医薬学雑誌 = Journal of traditional medicines, 2003Co-Authors: 直利 柴原, Nobuyasu Sekiya, Yutaka Shimada, Hirozo Goto, Naoki Mantani, Eiichi Tahara, Katsutoshi TerasawaAbstract:In order to confirm any effects ofKeishi－Bukuryo－Gan（KBG）on the autonomic nervous system，8healthy volun－ teers were evaluated with laser Doppler flowmetry and spectral analysis ofthe R－R intervals（RR）and systolic blood pressure（SBP）．After evaluation ofthe窟’oketsu”score and measurement ofthe electrophysiological parameters（PRE）， each subject was administere（1KBG or hot water。The same parameters were re－measured at15，30，45，60and90min－ utes after their administration to each subject．Changes in the parameters after the administration of KBG or hot water were investigated compared with PRE．In the experiment with KBG，skin blood flow（SBF）was increased at 90min，although it had been significantly decreased at15min。By spectral analysis，SBP－10w frequency（LF）and SBP．10w to high frequency ratio（L／H）were initially increased at15min，and then were significantly decreased at 60and90min．As for hot water，there were no significant changes in any of the parameters．It is known that SBF changes with sympathetic nervous activity，and SBP－LF and SBP－L／H reflect sympathetic nervous activity．These re－ sults suggest that KBG has certain effects on sympathetic nervous activity．
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Comparative efficacy of Keishi-Bukuryo-Gan and Pentoxifylline on RBC deformability in patients with “oketsu” syndrome
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2003Co-Authors: Hiroaki Hikiami, Nobuyasu Sekiya, Yutaka Shimada, Hirozo Goto, Naoko Hattori, Iwao Sakakibara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-Zhi-Fu-Ling-Wan) (KBG) is one of the prescriptions in Japanese traditional medicine for improving the "oketsu" syndrome, so-called blood stasis syndrome. "Oketsu" syndrome is an important pathological conception in Japanese traditional medicine and often accompanies cerebro-vascular disorders. Previously, we were able to reveal a deterioration of RBC (Red blood cell) deformability and viscoelasticity in patients with "oketsu" syndrome. The purpose of the present study was to evaluate whether KBG has an effect on RBC deformability in comparison with pentoxifylline (PXF). The subjects were 30 male patients with multiple lacunar infarctions. Eighteen patients (44-79 yrs, mean +/- SD, 66.1 +/- 10.7 yrs) were treated with 12 g of KBG daily for 4 weeks (KBG group). Twelve patients (59-78 yrs, 70.7 +/- 6.4 yrs) were treated with 300 mg of PXF daily for 4 weeks (PXF group). Based on the "oketsu" score, the patients of each group were divided into two subgroups, a non-"oketsu" group ("oketsu" score 20 points or less) and an "oketsu" group ("oketsu" score 21 points or higher). KBG had significant effects on RBC deformability as evaluated by filtration method. KBG also significantly increased intracellular ATP content, as did PXF. Moreover, KBG was more effective for patients with a more severe "oketsu" state. However, PXF was effective only in patients with "oketsu" syndrome, who might have deteriorated RBC deformability. In conclusion, the effect of KBG on RBC deformability was by no means inferior to PXF.
Nobuyasu Sekiya - One of the best experts on this subject based on the ideXlab platform.
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Oren-gedoku-to and Keishi-Bukuryo-Gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits.
Biological & pharmaceutical bulletin, 2005Co-Authors: Nobuyasu Sekiya, Yutaka Shimada, Takako Nakagawa, Hiroaki Hikiami, Naotoshi Shibahara, Mosaburo Kainuma, Kazufumi Kouta, Katsutoshi TerasawaAbstract:In this study, we examined whether the Kampo formulas Oren-gedoku-to (OGT, Huanglian-jie-du-tang in Chinese) and Keishi-Bukuryo-Gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) could prevent the progression of atherosclerosis in cholesterol-fed rabbit, an animal model for hypercholesterolemia in vivo. Twenty-four male Japanese white rabbits (2 kg body weight) were divided into four groups. The control group was fed standard rabbit chow containing 1% cholesterol, the OGT group was fed standard rabbit chow containing 1% cholesterol and 1% OGT, the KBG group was fed standard rabbit chow containing 1% cholesterol and 1% KBG, and the vitamin E group was fed standard rabbit chow containing 1% cholesterol and vitamin E (450 mg/1000 g). All four groups were kept on these diets for 8 weeks. At the end of the experiments, the percentage of surface area of the total thoracic aorta with visible plaque was significantly reduced in the OGT and KBG groups. The serum thiobarbituric acid reactive substances of the vitamin E group showed a significantly low value compared with the control group, whereas the serum lipid peroxide levels of the OGT and KBG groups were considerably lower than that of the control groups as well as that of the vitamin E group. Furthermore, the urinary 8-hydroxydeoxyguanosine levels of the OGT and KBG groups were considerably lower than that of the vitamin E group. These results suggest that OGT and KBG prevent the progression of atheromatous plaque by creating a sounder antioxidant defense system than vitamin E.
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Effects of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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effects of Keishi Bukuryo Gan on vascular function and hemorheological factors in spontaneously diabetic wbn kob rats
Phytomedicine, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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Protective effect of Keishi-Bukuryo-Gan and its constituent medicinal plants against nitric oxide donor-induced neuronal death in cultured cerebellar granule cells.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Yutaka Shimada, Nobuyasu Sekiya, Hirozo Goto, Naoki Mantani, Hiroaki Hikiami, Koichi Yokoyama, Eiichi Tahara, Katsutoshi TerasawaAbstract:Abstract Keishi-Bukuryo-Gan (Gui-Zhi-Fu-Ling-Wan) (KBG) is a traditional Chinese/Japanese medical (Kampo) formulation that has been administered to patients with “Oketsu” (blood stagnation) syndrome. In the process of neuronal cell death induced by brain ischemia, excessive generation of nitric oxide (NO) free radicals is implicated in the neurotoxicity. In the present study, we examined the protective effects of KBG and its constituent medicinal plants against NO donors, sodium nitroprusside (SNP) and 2,2′-(hydroxynitrosohydrazino) bis -ethanamine (NOC18)-induced neuronal death in cultured rat cerebellar granule cells (CGCs). MTT assay showed cell viability to be significantly increased by the addition of KBG extract (KBGE) (100 μg/ml), Cinnamomi Cortex extract (CCE) (3, 10 and 30 μg/ml), Paeoniae Radix extract (PRE) (100 μg/ml) and Moutan Cortex extract (MCE) (10 and 30 μg/ml) compared with exposure to SNP (30 μM, 24 h) only. Also, cell viability was significantly increased by the addition of KBGE (100 and 300 μg/ml), CCE (30 and 100 μg/ml), PRE (100 and 300 μg/ml) and MCE (30 and 100 μg/ml) compared with exposure to NOC18 (100 μM, 48 h) only. Persicae Semen extract and Hoelen extract did not protect against NO donor-induced neuronal death. These results suggest that KBG has protective effect against NO-mediated neuronal death in cultured CGCs and that it is derived from Cinnamomi Cortex, Paeoniae Radix and Moutan Cortex.
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effect of Keishi Bukuryo Gan on autonomic nervous activity
和漢医薬学雑誌 = Journal of traditional medicines, 2003Co-Authors: 直利 柴原, Nobuyasu Sekiya, Yutaka Shimada, Hirozo Goto, Naoki Mantani, Eiichi Tahara, Katsutoshi TerasawaAbstract:In order to confirm any effects ofKeishi－Bukuryo－Gan（KBG）on the autonomic nervous system，8healthy volun－ teers were evaluated with laser Doppler flowmetry and spectral analysis ofthe R－R intervals（RR）and systolic blood pressure（SBP）．After evaluation ofthe窟’oketsu”score and measurement ofthe electrophysiological parameters（PRE）， each subject was administere（1KBG or hot water。The same parameters were re－measured at15，30，45，60and90min－ utes after their administration to each subject．Changes in the parameters after the administration of KBG or hot water were investigated compared with PRE．In the experiment with KBG，skin blood flow（SBF）was increased at 90min，although it had been significantly decreased at15min。By spectral analysis，SBP－10w frequency（LF）and SBP．10w to high frequency ratio（L／H）were initially increased at15min，and then were significantly decreased at 60and90min．As for hot water，there were no significant changes in any of the parameters．It is known that SBF changes with sympathetic nervous activity，and SBP－LF and SBP－L／H reflect sympathetic nervous activity．These re－ sults suggest that KBG has certain effects on sympathetic nervous activity．
Yutaka Shimada - One of the best experts on this subject based on the ideXlab platform.
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Oren-gedoku-to and Keishi-Bukuryo-Gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits.
Biological & pharmaceutical bulletin, 2005Co-Authors: Nobuyasu Sekiya, Yutaka Shimada, Takako Nakagawa, Hiroaki Hikiami, Naotoshi Shibahara, Mosaburo Kainuma, Kazufumi Kouta, Katsutoshi TerasawaAbstract:In this study, we examined whether the Kampo formulas Oren-gedoku-to (OGT, Huanglian-jie-du-tang in Chinese) and Keishi-Bukuryo-Gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) could prevent the progression of atherosclerosis in cholesterol-fed rabbit, an animal model for hypercholesterolemia in vivo. Twenty-four male Japanese white rabbits (2 kg body weight) were divided into four groups. The control group was fed standard rabbit chow containing 1% cholesterol, the OGT group was fed standard rabbit chow containing 1% cholesterol and 1% OGT, the KBG group was fed standard rabbit chow containing 1% cholesterol and 1% KBG, and the vitamin E group was fed standard rabbit chow containing 1% cholesterol and vitamin E (450 mg/1000 g). All four groups were kept on these diets for 8 weeks. At the end of the experiments, the percentage of surface area of the total thoracic aorta with visible plaque was significantly reduced in the OGT and KBG groups. The serum thiobarbituric acid reactive substances of the vitamin E group showed a significantly low value compared with the control group, whereas the serum lipid peroxide levels of the OGT and KBG groups were considerably lower than that of the control groups as well as that of the vitamin E group. Furthermore, the urinary 8-hydroxydeoxyguanosine levels of the OGT and KBG groups were considerably lower than that of the vitamin E group. These results suggest that OGT and KBG prevent the progression of atheromatous plaque by creating a sounder antioxidant defense system than vitamin E.
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Effects of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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effects of Keishi Bukuryo Gan on vascular function and hemorheological factors in spontaneously diabetic wbn kob rats
Phytomedicine, 2004Co-Authors: Hirozo Goto, Nobuyasu Sekiya, Yutaka Shimada, Qiao Yang, Toshiaki Kogure, Naoki Mantani, Hiroaki Hikiami, Naotoshi Shibahara, Katsutoshi TerasawaAbstract:Keishi-Bukuryo-Gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-Bukuryo-Gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-Bukuryo-Gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-Bukuryo-Gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-Bukuryo-Gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-Bukuryo-Gan showed a protective effect against vascular injury in the susceptible rat.
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Protective effect of Keishi-Bukuryo-Gan and its constituent medicinal plants against nitric oxide donor-induced neuronal death in cultured cerebellar granule cells.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2004Co-Authors: Yutaka Shimada, Nobuyasu Sekiya, Hirozo Goto, Naoki Mantani, Hiroaki Hikiami, Koichi Yokoyama, Eiichi Tahara, Katsutoshi TerasawaAbstract:Abstract Keishi-Bukuryo-Gan (Gui-Zhi-Fu-Ling-Wan) (KBG) is a traditional Chinese/Japanese medical (Kampo) formulation that has been administered to patients with “Oketsu” (blood stagnation) syndrome. In the process of neuronal cell death induced by brain ischemia, excessive generation of nitric oxide (NO) free radicals is implicated in the neurotoxicity. In the present study, we examined the protective effects of KBG and its constituent medicinal plants against NO donors, sodium nitroprusside (SNP) and 2,2′-(hydroxynitrosohydrazino) bis -ethanamine (NOC18)-induced neuronal death in cultured rat cerebellar granule cells (CGCs). MTT assay showed cell viability to be significantly increased by the addition of KBG extract (KBGE) (100 μg/ml), Cinnamomi Cortex extract (CCE) (3, 10 and 30 μg/ml), Paeoniae Radix extract (PRE) (100 μg/ml) and Moutan Cortex extract (MCE) (10 and 30 μg/ml) compared with exposure to SNP (30 μM, 24 h) only. Also, cell viability was significantly increased by the addition of KBGE (100 and 300 μg/ml), CCE (30 and 100 μg/ml), PRE (100 and 300 μg/ml) and MCE (30 and 100 μg/ml) compared with exposure to NOC18 (100 μM, 48 h) only. Persicae Semen extract and Hoelen extract did not protect against NO donor-induced neuronal death. These results suggest that KBG has protective effect against NO-mediated neuronal death in cultured CGCs and that it is derived from Cinnamomi Cortex, Paeoniae Radix and Moutan Cortex.
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effect of Keishi Bukuryo Gan on autonomic nervous activity
和漢医薬学雑誌 = Journal of traditional medicines, 2003Co-Authors: 直利 柴原, Nobuyasu Sekiya, Yutaka Shimada, Hirozo Goto, Naoki Mantani, Eiichi Tahara, Katsutoshi TerasawaAbstract:In order to confirm any effects ofKeishi－Bukuryo－Gan（KBG）on the autonomic nervous system，8healthy volun－ teers were evaluated with laser Doppler flowmetry and spectral analysis ofthe R－R intervals（RR）and systolic blood pressure（SBP）．After evaluation ofthe窟’oketsu”score and measurement ofthe electrophysiological parameters（PRE）， each subject was administere（1KBG or hot water。The same parameters were re－measured at15，30，45，60and90min－ utes after their administration to each subject．Changes in the parameters after the administration of KBG or hot water were investigated compared with PRE．In the experiment with KBG，skin blood flow（SBF）was increased at 90min，although it had been significantly decreased at15min。By spectral analysis，SBP－10w frequency（LF）and SBP．10w to high frequency ratio（L／H）were initially increased at15min，and then were significantly decreased at 60and90min．As for hot water，there were no significant changes in any of the parameters．It is known that SBF changes with sympathetic nervous activity，and SBP－LF and SBP－L／H reflect sympathetic nervous activity．These re－ sults suggest that KBG has certain effects on sympathetic nervous activity．
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Skin temperature rise induced by calcitonin gene-related peptide in gonadotropin-releasing hormone analogue-treated female rats and alleviation by Keishi-Bukuryo-Gan, a Japanese herbal medicine.
Life sciences, 2005Co-Authors: Masamichi Noguchi, Yasushi Ikarashi, Mitsutoshi Yuzurihara, Yoshio Kase, Shuichi Takeda, Kenji Watanabe, Gregory A. Plotnikoff, Masaki AburadaAbstract:The effects of Keishi-Bukuryo-Gan on calcitonin gene-related peptide (CGRP)-induced elevation of skin temperature were investigated in gonadotropin-releasing hormone (GnRH) analogue-treated female rats. Leupline (1.0 mg/kg) as the GnRH analogue was subcutaneously (s.c.) injected into female rats. After Keishi-Bukuryo-Gan (100-1,000 mg/kg, p.o.) or 17beta-estradiol (0.010 mg/kg, s.c.) was administered to GnRH analogue-treated rats for 14 days, CGRP-induced skin temperature elevation, concentration of plasma 17beta-estradiol and pituitary gonadotropin (luteinizing hormone; LH, and follicle stimulating hormone; FSH) were measured. In addition, effects of 17beta-estradiol and Keishi-Bukuryo-Gan on the proliferation of estrogen-dependent human breast cancer (MCF-7) cells were investigated under in vitro conditions. GnRH analogue significantly lowered the concentrations of plasma 17beta-estradiol and pituitary gonadotropins. Tissue weights of the ovaries and uterus were also decreased by the analogue. Under the condition of estrogen deficiency, intravenous (i.v.) injection of exogenous CGRP (10 microg/kg) elevated the skin temperature of the hind paws more significantly than it did in sham-treated control rats. Estrogen supplementation inhibited this elevation of skin temperature with restoration of both the lowered plasma estrogen level and the decreased uterine weight in GnRH analogue-treated rats. On the other hand, Keishi-Bukuryo-Gan inhibited the elevation of skin temperature in a dose-dependent manner without restoring the plasma estrogen level and uterine weight. In addition, in an in vitro study, MCF-7 cells proliferated in a dose-dependent manner by the addition of 17beta-estradiol (10(-13)-10(-8) M) to the medium. However, Keishi-Bukuryo-Gan (10(-6)-10(-4) mg/ml) did not activate the MCF-7 cell proliferation. These results suggest that Keishi-Bukuryo-Gan, which does not exhibit estrogen activity, may be useful for the treatment of hot flashes in women who are undergoing medical ovariectomy with a GnRH analogue.
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Effects of the Japanese herbal medicine Keishi-Bukuryo-Gan and 17beta-estradiol on calcitonin gene-related peptide-induced elevation of skin temperature in ovariectomized rats
The Journal of endocrinology, 2003Co-Authors: Masamichi Noguchi, Yasushi Ikarashi, Mitsutoshi Yuzurihara, Yoshio Kase, Masaki Aburada, Jt Chen, Satoshi Takeda, A IshigeAbstract:The effects of a Japanese herbal medicine, Keishi-BukuryoGan, and 17-estradiol on calcitonin gene-related peptide (CGRP)-induced elevation of skin temperature were investigated in ovariectomized (OVX) rats. Ovariectomy not only potentiated CGRP-induced elevation of skin temperature and arterial vasorelaxation but also induced a lower concentration of endogenous CGRP in plasma and up-regulation of arterial CGRP receptors, suggesting that lowered CGRP in plasma due to ovarian hormone deficiency increases the number of CGRP receptors and consequently amplifies the stimulatory effects of CGRP to elevate skin temperature. Oral Keishi-Bukuryo-Gan (100‐ 1000 mg/kg, once a day for 7 days) restored a series of CGRP-related responses observed in OVX rats by normalizing plasma CGRP levels in a dose-dependent manner as effectively as s.c. injection. 17-estradiol (0·010 mg/kg, once a day for 7 days). However, KeishiBukuryo-Gan did not affect the lower concentration of plasma estradiol and the decreased uterine weight due to ovariectomy, although the hormone replacement of 17-estradiol restored them. These results suggest that Keishi-Bukuryo-Gan, which does not confer estrogen activity on plasma, may be useful for the treatment of hot flashes in patients for whom estrogen replacement therapy is contraindicated, as well as menopausal women.
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Effects of 17β-Estradiol and the Japanese Herbal Medicine Keishi-Bukuryo-Gan on the Release and Synthesis of Calcitonin Gene-Related Peptide in Ovariectomized Rats
Journal of pharmacological sciences, 2003Co-Authors: Masamichi Noguchi, Yasushi Ikarashi, Mitsutoshi Yuzurihara, Yoshio Kase, Shuichi Takeda, Masaki AburadaAbstract:The purpose of this study is to clarify the effects of 17beta-estradiol and the Japanese herbal medicine Keishi-Bukuryo-Gan on the release and synthesis of calcitonin gene-related peptide (CGRP) in ovariectomized (OVX) rats. The effect of ovariectomy on the release or synthesis was evaluated by measuring CGRP concentration in plasma after capsaicin (1.0 mg/kg, i.p.) injection or by measuring CGRP concentration and its mRNA expression in dorsal root Ganglia in OVX rats. Ovariectomy attenuated the capsaicin-evoked increase in plasma concentration of CGRP, which was restored by treatment with 17beta-estradiol (0.010 mg/kg, s.c.) or Keishi-Bukuryo-Gan (1000 mg/kg, p.o.) for 7 days after ovariectomy. However, no significant differences were observed in the CGRP concentration and the mRNA expression of dorsal root Ganglia by treating the rats with ovariectomy, 17 beta-estradiol, and Keishi-Bukuryo-Gan. These results suggest not only that estrogen deficiency attenuates CGRP release but also that 17beta-estradiol or Keishi-Bukuryo-Gan normalizes the attenuated release process.