The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Suman Lakhanpaul - One of the best experts on this subject based on the ideXlab platform.
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phloem sap analysis of schleichera oleosa lour oken butea monosperma lam taub and ziziphus mauritiana lam and hemolymph of Kerria lacca kerr using hplc and tandem mass spectrometry
Physiology and Molecular Biology of Plants, 2013Co-Authors: Amit Vashishtha, Sandeep Kaushik, K K Sharma, Brijesh Rathi, Suman LakhanpaulAbstract:Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent’s viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6–85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4–39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %–69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %–35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect’s endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding.
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mouthparts and stylet penetration of the lac insect Kerria lacca kerr hemiptera tachardiidae
Arthropod Structure & Development, 2012Co-Authors: Ayashaa Ahmad, Sandeep Kaushik, V V Ramamurthy, Suman Lakhanpaul, R Ramani, K K Sharma, Ambarish Sharan VidyarthiAbstract:Hitherto less known aspects on mouthpart morphology and penetration mechanism of the lac insect Kerria lacca have been explored. Unique details of the mouthparts, i.e. morphology of labium and stylets and salivary sheath have been brought out. The gross morphology of the mouthparts though resembled other plant sucking homopterans; a two-segmented labium with symmetrically distributed six pairs of contact-chemoreceptors on its surface was distinct; the mandibular stylets had serrations on its extreme apical region, while the maxillary stylets had their external surface smooth with parallel longitudinal grooves on their inner surface. Formation of flanges, salivary sheath and penetration pathway observed along with probing and penetration of the stylets intracellularly up to the phloem cells, as illustrated herein, are the addition to the existing knowledge on the structural details of the mouthparts and the feeding behavior thereupon.
Vincent Verney - One of the best experts on this subject based on the ideXlab platform.
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Molecular changes during natural biopolymer ageing - The case of shellac
Polymer Degradation and Stability, 2012Co-Authors: Christian Coelho, Raviteja Nanabala, Matthieu Ménager, Sophie Commereuc, Vincent VerneyAbstract:Shellac is derived from the resinous secretion of the lac insect (Kerria lacca), mainly found in India. This biopolymer is made of natural single and polyesters of aliphatic acids and sesquiterpenoid acids. Ageing was investigated through interdisciplinary approaches to understand the behaviour and the interactions of macromolecules in the biological system. During ageing the viscoelastic properties of the polymer evolved in the sense of crosslinking of the macromolecules. The fate of the shellac backbone molecules was assessed. It was found that hydroxy fatty acid such as aleuritic acid could be a factor in the changes of the polymer consistency. Aleuritic acid could be freed from the polymer during its ageing after deesterification and could participate through hydrogen bonding in the biopolymer crosslinking observed by melt rheology.
K K Sharma - One of the best experts on this subject based on the ideXlab platform.
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18S rDNA PCR based detection of hymenopteran parasitoids in the Indian Lac insect, Kerria lacca [Kerr]
NISCAIR-CSIR India, 2019Co-Authors: Kandasamy Thamilarasi, K K Sharma, Roy Tamoshree, Mohanasundaram A, Ramani RAbstract:526-531The Indian lac insect, Kerria lacca (Kerr) is an economically important insect yielding resin, dye and wax. Aprostocetus purpureus and Tachardiaephagus tachardiae are the two most important parasitoids of lac insect. Timely detection of the parasitoids infection is essential for their control. Host dissection and caging of adult lac insects are the existing methods for detection of parasitoids in lac culture. Host dissection is laborious, whereas caging helps to detect the presence of only adult but not immature stages of parasitoids. Hence in this study, a PCR based approach has been developed for detection of these two important parasitoids in lac insects. Primers were designed from the clustalW alignment of 18S rDNA sequences of parasitoids and lac insects. Subsequently, specificity of the primers was checked by PCR i.e., A. purpureus and T. tachardiae specific primers amplified only the respective parasitoid DNA but not lac insect and another parasitoid DNA. The PCR methods thus developed were also found to work with field collected infected lac insect samples and were able to differentiate infected lac insects from the uninfected ones
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phloem sap analysis of schleichera oleosa lour oken butea monosperma lam taub and ziziphus mauritiana lam and hemolymph of Kerria lacca kerr using hplc and tandem mass spectrometry
Physiology and Molecular Biology of Plants, 2013Co-Authors: Amit Vashishtha, Sandeep Kaushik, K K Sharma, Brijesh Rathi, Suman LakhanpaulAbstract:Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent’s viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6–85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4–39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %–69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %–35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect’s endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding.
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mouthparts and stylet penetration of the lac insect Kerria lacca kerr hemiptera tachardiidae
Arthropod Structure & Development, 2012Co-Authors: Ayashaa Ahmad, Sandeep Kaushik, V V Ramamurthy, Suman Lakhanpaul, R Ramani, K K Sharma, Ambarish Sharan VidyarthiAbstract:Hitherto less known aspects on mouthpart morphology and penetration mechanism of the lac insect Kerria lacca have been explored. Unique details of the mouthparts, i.e. morphology of labium and stylets and salivary sheath have been brought out. The gross morphology of the mouthparts though resembled other plant sucking homopterans; a two-segmented labium with symmetrically distributed six pairs of contact-chemoreceptors on its surface was distinct; the mandibular stylets had serrations on its extreme apical region, while the maxillary stylets had their external surface smooth with parallel longitudinal grooves on their inner surface. Formation of flanges, salivary sheath and penetration pathway observed along with probing and penetration of the stylets intracellularly up to the phloem cells, as illustrated herein, are the addition to the existing knowledge on the structural details of the mouthparts and the feeding behavior thereupon.
Sandeep Kaushik - One of the best experts on this subject based on the ideXlab platform.
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phloem sap analysis of schleichera oleosa lour oken butea monosperma lam taub and ziziphus mauritiana lam and hemolymph of Kerria lacca kerr using hplc and tandem mass spectrometry
Physiology and Molecular Biology of Plants, 2013Co-Authors: Amit Vashishtha, Sandeep Kaushik, K K Sharma, Brijesh Rathi, Suman LakhanpaulAbstract:Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent’s viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6–85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4–39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %–69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %–35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect’s endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding.
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mouthparts and stylet penetration of the lac insect Kerria lacca kerr hemiptera tachardiidae
Arthropod Structure & Development, 2012Co-Authors: Ayashaa Ahmad, Sandeep Kaushik, V V Ramamurthy, Suman Lakhanpaul, R Ramani, K K Sharma, Ambarish Sharan VidyarthiAbstract:Hitherto less known aspects on mouthpart morphology and penetration mechanism of the lac insect Kerria lacca have been explored. Unique details of the mouthparts, i.e. morphology of labium and stylets and salivary sheath have been brought out. The gross morphology of the mouthparts though resembled other plant sucking homopterans; a two-segmented labium with symmetrically distributed six pairs of contact-chemoreceptors on its surface was distinct; the mandibular stylets had serrations on its extreme apical region, while the maxillary stylets had their external surface smooth with parallel longitudinal grooves on their inner surface. Formation of flanges, salivary sheath and penetration pathway observed along with probing and penetration of the stylets intracellularly up to the phloem cells, as illustrated herein, are the addition to the existing knowledge on the structural details of the mouthparts and the feeding behavior thereupon.
Christian Coelho - One of the best experts on this subject based on the ideXlab platform.
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Molecular changes during natural biopolymer ageing - The case of shellac
Polymer Degradation and Stability, 2012Co-Authors: Christian Coelho, Raviteja Nanabala, Matthieu Ménager, Sophie Commereuc, Vincent VerneyAbstract:Shellac is derived from the resinous secretion of the lac insect (Kerria lacca), mainly found in India. This biopolymer is made of natural single and polyesters of aliphatic acids and sesquiterpenoid acids. Ageing was investigated through interdisciplinary approaches to understand the behaviour and the interactions of macromolecules in the biological system. During ageing the viscoelastic properties of the polymer evolved in the sense of crosslinking of the macromolecules. The fate of the shellac backbone molecules was assessed. It was found that hydroxy fatty acid such as aleuritic acid could be a factor in the changes of the polymer consistency. Aleuritic acid could be freed from the polymer during its ageing after deesterification and could participate through hydrogen bonding in the biopolymer crosslinking observed by melt rheology.
