The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
J B Hendricks - One of the best experts on this subject based on the ideXlab platform.
-
effect of fixation time and microwave oven heating time on retrieval of the Ki 67 Antigen from paraffin embedded tissue
Journal of Histochemistry and Cytochemistry, 1993Co-Authors: S Munakata, J B HendricksAbstract:Microwave oven heating has been employed for retrieval of Antigens from formalin-fixed, paraffin-embedded tissue for immunohistochemical staining. Recently, a Ki-67 equivalent murine monoclonal antibody was generated which can detect tumor proliferative activity in routinely processed tissue with microwave oven heating. We assessed the effect of fixation time (4, 24, or 48 hr) and microwave oven heating time (7, 14, 21, 28, 35, or 49 min) on retrieval of the Ki-67 Antigen from tonsil tissue. Ki-67 staining was quantitated by image analysis. Owing to the heterogeneity of Ki-67 staining within and between germinal centers, we employed a measurement technique that averages staining across the germinal centers of each section. Good Ki-67 immunostaining was observed for all microwave oven heating times in tissue fixed for 4 hr. In contrast, poor immunostaining was observed in tissue fixed for 48 hr unless a heating time of at least 14 min was used. Tonsil fixed for 24 or 48 hr showed a significant increase in ...
-
effect of fixation time and microwave oven heating time on retrieval of the Ki 67 Antigen from paraffin embedded tissue
Journal of Histochemistry and Cytochemistry, 1993Co-Authors: S Munakata, J B HendricksAbstract:Microwave oven heating has been employed for retrieval of Antigens from formalin-fixed, paraffin-embedded tissue for immunohistochemical staining. Recently, a Ki-67 equivalent murine monoclonal antibody was generated which can detect tumor proliferative activity in routinely processed tissue with microwave oven heating. We assessed the effect of fixation time (4, 24, or 48 hr) and microwave oven heating time (7, 14, 21, 28, 35, or 49 min) on retrieval of the Ki-67 Antigen from tonsil tissue. Ki-67 staining was quantitated by image analysis. Owing to the heterogeneity of Ki-67 staining within and between germinal centers, we employed a measurement technique that averages staining across the germinal centers of each section. Good Ki-67 immunostaining was observed for all microwave oven heating times in tissue fixed for 4 hr. In contrast, poor immunostaining was observed in tissue fixed for 48 hr unless a heating time of at least 14 min was used. Tonsil fixed for 24 or 48 hr showed a significant increase in percentage positive nuclear area after microwave times of 14 or 21 min. Prolonged heating time (up to 49 min) had no effect on the quality of Ki-67 staining in tissue fixed for 4 or 48 hr.
S Munakata - One of the best experts on this subject based on the ideXlab platform.
-
effect of fixation time and microwave oven heating time on retrieval of the Ki 67 Antigen from paraffin embedded tissue
Journal of Histochemistry and Cytochemistry, 1993Co-Authors: S Munakata, J B HendricksAbstract:Microwave oven heating has been employed for retrieval of Antigens from formalin-fixed, paraffin-embedded tissue for immunohistochemical staining. Recently, a Ki-67 equivalent murine monoclonal antibody was generated which can detect tumor proliferative activity in routinely processed tissue with microwave oven heating. We assessed the effect of fixation time (4, 24, or 48 hr) and microwave oven heating time (7, 14, 21, 28, 35, or 49 min) on retrieval of the Ki-67 Antigen from tonsil tissue. Ki-67 staining was quantitated by image analysis. Owing to the heterogeneity of Ki-67 staining within and between germinal centers, we employed a measurement technique that averages staining across the germinal centers of each section. Good Ki-67 immunostaining was observed for all microwave oven heating times in tissue fixed for 4 hr. In contrast, poor immunostaining was observed in tissue fixed for 48 hr unless a heating time of at least 14 min was used. Tonsil fixed for 24 or 48 hr showed a significant increase in ...
-
effect of fixation time and microwave oven heating time on retrieval of the Ki 67 Antigen from paraffin embedded tissue
Journal of Histochemistry and Cytochemistry, 1993Co-Authors: S Munakata, J B HendricksAbstract:Microwave oven heating has been employed for retrieval of Antigens from formalin-fixed, paraffin-embedded tissue for immunohistochemical staining. Recently, a Ki-67 equivalent murine monoclonal antibody was generated which can detect tumor proliferative activity in routinely processed tissue with microwave oven heating. We assessed the effect of fixation time (4, 24, or 48 hr) and microwave oven heating time (7, 14, 21, 28, 35, or 49 min) on retrieval of the Ki-67 Antigen from tonsil tissue. Ki-67 staining was quantitated by image analysis. Owing to the heterogeneity of Ki-67 staining within and between germinal centers, we employed a measurement technique that averages staining across the germinal centers of each section. Good Ki-67 immunostaining was observed for all microwave oven heating times in tissue fixed for 4 hr. In contrast, poor immunostaining was observed in tissue fixed for 48 hr unless a heating time of at least 14 min was used. Tonsil fixed for 24 or 48 hr showed a significant increase in percentage positive nuclear area after microwave times of 14 or 21 min. Prolonged heating time (up to 49 min) had no effect on the quality of Ki-67 staining in tissue fixed for 4 or 48 hr.
Maciej Zabel - One of the best experts on this subject based on the ideXlab platform.
-
nogo b receptor expression correlates negatively with malignancy grade and Ki 67 Antigen expression in invasive ductal breast carcinoma
Anticancer Research, 2014Co-Authors: Bartosz Pula, Mateusz Olbromski, Tomasz Owczarek, Aleksandra Ambicka, Wojciech Witkiewicz, Maciej Ugorski, Janusz Rys, Maciej ZabelAbstract:BACKGROUND Nogo-B receptor (NgBR) has been shown to be involved in endothelial cell chemotaxis and morphogenesis. However, few studies analyzing its expression in cancer cells have been performed. MATERIALS AND METHODS We examined NgBR expression in 233 patients with invasive ductal breast carcinoma (IDC) and corresponding non-malignant breast tissues (NMBT) on mRNA (real-time polymerase chain reaction) and protein levels (immunohistochemistry; IHC and western-blot analysis). NgBR expression was found also analyzed in breast cancer cell lines of varying invasiveness. RESULTS NgBR expression was increased in IDC compared to NMBT on the mRNA (p=0.0007) and protein level (p=0.018). NgBR expression decreased significantly with IDC malignancy grade and correlated negatively with the Ki-67 Antigen expression (r=-0.18; p=0.0005). High NgBR mRNA expression was associated with estrogen receptor negativity (p=0.0023) and the triple-negative phenotype of the tumors (p=0.0129). CONCLUSION NgBR may be involved in IDC development, however, its role in its progression requires further research.
-
immunohistochemical evaluation of metallothionein mcm 2 and Ki 67 Antigen expression in tumors of the adrenal cortex
Anticancer Research, 2008Co-Authors: Artur Szajerka, Piotr Dziegiel, Maciej Zabel, Tobiasz Szajerka, Jacek Winowski, Zygmunt GrzebieniakAbstract:. Background: The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these markers of proliferation and between these markers and tumor diameter. Materials and Methods: The expression of MT, Mcm-2 and Ki-67 was assessed by immunochemistry in forty-eight adrenocortical adenomas, six adrenocortical carcinomas and eleven normal adrenal cortex tissue samples. Results: The expressions of MT, Mcm-2 and Ki-67 in the adrenocortical carcinomas were significantly higher than in the adenomas and normal tissue (p<0.05). The levels of Mcm-2 were also higher in the adrenocortical adenomas compared to the normal tissue (p<0.05). The Mcm-2 expression showed a positive correlation to the expression of MT in the adrenocortical carcinomas (r=0.773; p<0.05) and to the expression of Ki-67 in the adrenocortical adenomas (r=0.432; p<0.05). The malignant tumor diameter was positively correlated with the MT and Mcm-2 expressions (r=0.766, p<0.05 and r=0.620, p<0.05, respectively). Conclusion: The assessment of Mcm-2 expression seems to be of special importance as a marker of adrenocortical dysplasia and a reliable indicator of malignancy in suspicious masses of the adrenal cortex.
-
prognostic significance of Ki 67 Antigen expression in non hodgKin s lymphomas
Anticancer Research, 2008Co-Authors: Katarzyna Szczuraszek, Grzegorz Mazur, Michal Jelen, Piotr Dziegiel, Pawel Surowiak, Maciej ZabelAbstract:Background Non-HodgKin's lymphomas (NHLs) are malignant tumours of the lymphoid system. Various risk factors have been described which are helpful in diagnosing, monitoring of the clinical course and predicting survival time of the patients. Proliferative activity of the tumour, measured by expression of Ki-67 Antigen, is linked to the tumour proliferation rate and represents a recognised prognostic index in various tumours. In this study, the prognostic and predictive value of Ki-67 expression in NHL was evaluated. Patients and methods Expression of Ki-67 was examined using an immunohistochemical technique in archival paraffin-embedded sections taken from 56 previously untreated patients with diagnosed primary NHL. An attempt was made to test correlation between Ki-67 expression on one hand and clinical parameters of the patients and their survival on the other. Results Expression of Ki-67 Antigen was noted in 75% of the tumour cases. In the group manifesting higher Ki-67 indices, survival of the patients was significantly shorter (p = 0.03). No significant correlation could be detected between Ki-67 Antigen expression and clinical or pathological parameters of the patients. Conclusion The results demonstrate that the most cases of NHL display the expression of Ki-67. Moreover, shortened survival was noted in patients with high expression of Ki-67.
-
prognostic significance of metallothionein p53 protein and Ki 67 Antigen expression in laryngeal cancer
Anticancer Research, 2007Co-Authors: Wojciech Pastuszewski, Piotr Dziegiel, Marzena Podhorskaokolow, Tomasz Krecicki, Urszula Ciesielska, Elzbieta Gorzynska, Maciej ZabelAbstract:Background: This study aimed at the evaluation of the expression of metallothionein (MT) in laryngeal carcinoma and its correlation with the expression of Ki-67 Antigen and p53 protein and selected clinical and pathological variables in view of their potential prognostic significance. Materials and Methods: Sixty-five laryngeal cancer patients were retrospectively analysed. Expression of MT, Ki-67 and p53 in tumour tissue samples were assessed by immunohistochemistry. Results: In laryngeal cancer a significantly augmented expression of MT, Ki-67 and p53 was noted, as compared to the control group (p<0.001) and a significantly increased expression of MT in low malignancy tumours (G1) as compared to the control group (p<0.001). The expression of Ki-67 Antigen was positively correlated with the expression of p53 protein (r=0.477; p<0.05) and expression of either marker was positively correlated with malignancy grade (r=0.47, p<0.05; r=0.31, p<0.05; for Ki-67 and p53, respectively). Shortened survival was noted in patients with high expression of Ki-67 Antigen and p53 protein. Conclusion: The intensity of MT expression was not related to prognosis in laryngeal cancer. Nevertheless, it may provide a significant index indicating the malignant transformation of benign lesions of laryngeal epithelium. According to current data of the International Agency for
-
prognostic significance of augmented metallothionein mt expression correlated with Ki 67 Antigen expression in selected soft tissue sarcomas
Histology and Histopathology, 2005Co-Authors: Piotr Dziegiel, W Salwazurawska, J Zurawski, Andrzej Wojnar, Maciej ZabelAbstract:Summary. In soft tissue sarcomas, the most important prognostic criteria include extent of malignancy (G), size of the tumour and intensity of Ki-67 Antigen expression. In recent times expression of metallothionein (MT) in cells of some malignant processes of epithelial origin was found to correlate with intensity of Ki-67 Antigen expression and to carry a possible prognostic significance. The present study aimed at a demonstration of prognostic value of MT expression and at comparing it with Ki-67 Antigen expression and G grade in selected soft tissue sarcomas. Immunohistochemical studies were performed on paraffin sections in 54 cases of malignant fibrous histiocytoma (MFH), 18 cases of liposarcoma and 20 cases of synovial sarcoma. The extent of MT and Ki-67 Antigen expression was evaluated and an attempt was made to correlate the results with each other and with grade of the tumour. Expression of MT was evident both in the cytoplasm and in cell nuclei of all studied sarcomas. The most pronounced MT expression was noted in MFH-type tumours. The extent of Ki-67 Antigen expression was similar in MFH and liposarcoma and was the lowest in synovial sarcoma. In MFH, liposarcoma and synovial sarcoma a pronounced positive correlation was documented between expression of MT and Ki-67 Antigen (r=0.85; p<0.001; r=0.93, p<0.0001; r=0.79, p<0.0001). In all types of the tumours a positive relation was detected between MT expression, expression of Ki67 and G grade of malignancy in the tumour. Moreover, patients with higher MT expression in the studied tumours demonstrated a shorter survival. MT expression in soft tissue tumours of MFH, liposarcoma and synovial sarcoma type strongly correlated with intensity of proliferation (Ki-67) and G grade and could be useful in defining the extent of malignancy and in prognostic appraisal in the tumours.
Joanna Jelenkrzeszewska - One of the best experts on this subject based on the ideXlab platform.
-
mcm 2 protein expression predicts prognosis better than Ki 67 Antigen in oral cavity squamocellular carcinoma
Anticancer Research, 2006Co-Authors: Jolanta Szelachowska, Michal Jelen, Piotr Dziegiel, Joanna Jelenkrzeszewska, Rafal Matkowski, Agnieszka Pomiecko, Barbara Spytkowska, Maria Jagas, Iwona Gisterek, Jan KornafelAbstract:Background: The present study aimed at evaluating the expression intensities of the Mcm-2 protein and Ki-67 Antigen in squamocellular carcinomas of the oral cavity and comparing their prognostic value. Materials and Methods: Forty-nine patients, operated on and treated with radiotherapy for carcinoma of the oral cavity floor and/or the mobile part of the tongue, were retrospectively analyzed. Results: A significant positive correlation was noted between the expression of Mcm-2 protein and that of the Ki-67 Antigen, as well as an absence of such correlations with the remaining examined factors. A significant correlation with worse disease- specific survival period (DSS) in the group of patients demonstrating Mcm-2 protein expression in over 10% of cancer cells was detected (5-year cumulative DSS 50% vs. 76%). Conclusion: The results suggested that the expression of Mcm-2 protein may be used as a prognostic factor in patients with squamocellular carcinoma of the oral cavity. Cancer of the oral cavity comprises less than 4% of total morbidity from malignant tumors in Europe. The squamocellular type accounts for over 90% of cancer diagnosed in the oral cavity. Most tumors develop multifocally and exhibit a marked tendency to relapse. The prognosis depends on the primary location of the tumor, its grade of malignancy (G), type of invasion and involvement of lymph nodes (1, 2). The proliferation markers currently applied, such as Ki-67, provide only a restricted picture of the involvement of the cells in the cell cycle (3-9). Moreover, the value of Ki-67 as a prognostic factor remains to be fully documented and the results of studies which evaluated the relationship between cell proliferation and the clinical course of the disease are contradictory (10-16). This can be attributed, to some extent, to the fact that the function of the Ki-67 protein remains unknown and the protein is not a key element in cell proliferation (17). The expression of Ki-67 may also appear when the synthesis of DNA is blocked or in cells undergoing apoptosis (7).
-
epidermal growth factor receptor egfr proliferating cell nuclear Antigen pcna and Ki 67 Antigen in laryngeal epithelial lesions
Oral Oncology, 1999Co-Authors: Tomasz Krecicki, Michal Jelen, Maria Zalesskakrecicka, Jerzy Rak, Teresa Szkudlarek, Joanna JelenkrzeszewskaAbstract:The epidermal growth factor receptor (EGFR), proliferating cell nuclear Antigen (PCNA) and Ki-67 immunostaining in paraffin sections from 154 cases of laryngeal squamous cell carcinoma, 25 specimens of normal and hyperplastic laryngeal epithelium, and 21 preneoplastic lesions was examined. The difference of EGFR expression and PCNA score between laryngeal cancer and dysplastic lesions vs normal and hyperplastic epithelium was significant. There was significant difference in Ki-67 score between malignant vs premalignant lesions and normal and hyperplastic epithelium. There was significant correlation between PCNA score and histopathological grading of the tumour. The highest PCNA expression was detected in stage G3. Our findings have shown that PCNA and Ki-67 staining can be used as a marker of cell proliferative activity in laryngeal epithelial lesions. EGFR, PCNA and Ki-67 expression correlate with severity of laryngeal lesions. PCNA immunostaining can aid in estimating the histological grade of malignant lesions.
Tetsu Takahashi - One of the best experts on this subject based on the ideXlab platform.
-
the relationship of the histologic grade at the deep invasive front and the expression of Ki 67 Antigen and p53 protein in oral squamous cell carcinoma
Journal of Oral Pathology & Medicine, 2005Co-Authors: Hideo Kurokawa, Min Zhang, Shinobu Matsumoto, Yoshihiro Yamashita, Toshiko Tanaka, Taiki Tomoyose, Hirofumi Takano, Katsuyuki Funaki, Hiroshi Fukuyama, Tetsu TakahashiAbstract:Background: Although many histopathologic characteristics of oral squamous cell carcinoma (O-SCC) have been identified as prognostic factors, accurate, and unequivocal factors have not been clearly identified. The purpose of this study was to evaluate a potential association between the histologic grade of malignancy at the deep invasive front and the expression of Ki-67 Antigen and p53 protein in O-SCC. Methods: The expression of Ki-67 Antigen and p53 at the invasive tumor front area of O-SCC was examined by immunohistochemistry of archived tissue from 62 cases. The mean age of patients was 60.7 years (range: 37–89) and the male–female ratio was 1.6:1 (38 men, 24 women). There were 20, 17, 14, and 11 cases classified as stage I to stage IV, respectively. The correlation between the intensity of immunostaining for Ki-67 Antigen and p53 and the histologic grade of malignancy at the deep invasive front (invasive front grade, IFG) was analyzed. The expression of Ki-67 Antigen and p53 in normal oral epithelia (10 cases) was also investigated. Results: The mean Ki-67 labeling index (LI) in the O-SCC samples was 32.8 ± 12.0% (n = 62). The mean total score of IFG (IFG score) was 9.1 ± 2.7 points (n = 62). There was a significant linear correlation between the IFG score and the Ki-67 Antigen (γ = 0.651, R2 = 0.596, P < 0.0001). Of 50 tumors examined, 27 (54.0%) exhibited p53-positive nuclear immunostaining. The staining patterns for Ki-67 Antigen and p53 were similar. Both Ki-67-LI and p53-positive status were significantly correlated with the IFG scores. Conclusion: The findings of this study demonstrate that overexpression of Ki-67 Antigen and p53 at the deep tumor invasive front of O-SCC is associated with histologic grade of malignancy.
-
immunohistochemical study of syndecan 1 down regulation and the expression of p53 protein or Ki 67 Antigen in oral leukoplaKia with or without epithelial dysplasia
Journal of Oral Pathology & Medicine, 2003Co-Authors: Hideo Kurokawa, Min Zhang, Shinobu Matsumoto, Yoshihiro Yamashita, Taiki Tomoyose, Hiroshi Fukuyama, Tomoyuki Murata, Tetsu TakahashiAbstract:Background: LeukoplaKia is an oral pre-cancerous lesion that sometimes develops into squamous cell carcinoma. Therefore, leukoplaKia with epithelial dysplasia is useful for studying carcinogenesis at the cellular level. The purpose of this study was to evaluate a potential association between the loss of syndecan-1 expression and the expression of p53 protein and Ki-67 Antigen, and to identify reliable markers for predicting malignant changes in oral leukoplaKia with epithelial dysplasia. Methods: Changes in the expression of syndecan-1, p53, and Ki-67 were examined immunohistochemically in 43 cases of oral leukoplaKia with or without epithelial dysplasia. The subjects were categorized as: none, 13 cases; mild dysplasia, 5 cases; moderate dysplasia, 17 cases; and severe dysplasia, 8 cases. The expression of these molecules in normal oral epithelia (22 cases) was also investigated. Results: Strong syndecan-1 expression was observed on the surface of keratinocytes in normal epithelium. Immunopositivity was lost gradually as the extent of epithelial dysplasia increased. In normal epithelium, p53 and Ki-67 appeared mainly in the basal cell layer, while they were more widely distributed in leukoplaKia. Specifically, significant changes were observed in the labeling index of p53 and Ki-67 in leukoplaKia as epithelial dysplasia progressed from mild to moderate or severe. Conclusion: Our results reveal that overexpression of p53 protein and Ki-67 Antigen, and down-regulation of syndecan-1 expression in the lower part of the epithelium, are associated with dysplastic changes. Therefore, the down-regulation of syndecan-1 expression may be the most important reliable marker for dysplastic changes.
