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Pablo Yagupsky - One of the best experts on this subject based on the ideXlab platform.
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Kingella negevensis shares multiple putative virulence factors with Kingella kingae
PLOS ONE, 2020Co-Authors: Eric A Porsch, Joseph W St Geme, Pablo YagupskyAbstract:Kingella negevensis is a newly described gram-negative bacterium in the Neisseriaceae family and is closely related to Kingella kingae, an important cause of pediatric osteoarticular infections and other invasive diseases. Like K. kingae, K. negevensis can be isolated from the oropharynx of young children, although at a much lower rate. Due to the potential for misidentification as K. kingae, the burden of disease due to K. negevensis is currently unknown. Similarly, there is little known about virulence factors present in K. negevensis and how they compare to virulence factors in K. kingae. Using a variety of approaches, we show that K. negevensis produces many of the same putative virulence factors that are present in K. kingae, including a polysaccharide capsule, a secreted exopolysaccharide, a Knh-like trimeric autotransporter, and type IV pili, suggesting that K. negevensis may have significant pathogenic potential.
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diagnosing Kingella kingae infections in infants and young children
Expert Review of Anti-infective Therapy, 2017Co-Authors: Pablo YagupskyAbstract:INTRODUCTION Kingella kingae is currently recognized as the prime etiology of skeletal system infections in children aged 6-48 months. The organism is notoriously fastidious, its growth is inhibited by synovial fluid and bone exudates, and its presence in clinical specimens is commonly missed by traditional culture methods. Areas covered: The present review discusses the use of improved laboratory methods to detect the organism in normally sterile body fluids, exudates, and upper respiratory tract specimens. Expert commentary: While inoculation of joint and bone exudates into blood culture vials dilutes the concentration of detrimental factors and significantly improves the isolation of the organism, novel PCR-based assays have enhanced sensitivity, shortened the time-to-detection of K. kingae from 3-4 days to <24 h, and enabled the bacteriological diagnosis in patients being administered antibiotic therapy. PCR-based assays that amplify the 16S rRNA gene results in a 200% improvement in the diagnosis of the organism compared to culture, whereas the use of real-time PCR tests that target K. kingae-specific DNA sequences increases the detection rate by a five-fold factor and reduces the fraction of culture-negative septic arthritis and osteomyelitis in infants and young children.
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diagnosing Kingella kingae infections in infants and young children
Expert Review of Anti-infective Therapy, 2017Co-Authors: Pablo YagupskyAbstract:Introduction: Kingella kingae is currently recognized as the prime etiology of skeletal system infections in children aged 6–48 months. The organism is notoriously fastidious, its growth is inhibit...
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the type a and type b polysaccharide capsules predominate in an international collection of invasive Kingella kingae isolates
mSphere, 2017Co-Authors: Eric A Porsch, Joseph W St Geme, Pablo Yagupsky, Kimberly StarrAbstract:ABSTRACT Kingella kingae is an encapsulated Gram-negative bacterium and an important etiology of osteoarticular infections in young children. A recent study examining a diverse collection of carrier and invasive K. kingae isolates from Israel revealed four distinct polysaccharide capsule types. In this study, to obtain a global view of K. kingae capsule type diversity, we examined an international collection of isolates using a multiplex PCR approach. The collection contained all four previously identified capsule types and no new capsule types. Over 95% of invasive isolates in the collection were type a or type b, similar to the findings in Israel. These results suggest that the type a and type b polysaccharide capsules may have enhanced pathogenic properties or may mark clonal groups of strains with specific virulence genes. In addition, they raise the possibility that a vaccine containing the type a and type b capsules might be an effective approach to preventing K. kingae disease. IMPORTANCE Kingella kingae has emerged as a significant cause of septic arthritis, osteomyelitis, and bacteremia in young children. A recent study examining a diverse collection of K. kingae isolates from Israel revealed four different polysaccharide capsule types in this species, designated types a to d. To determine the global distribution of K. kingae capsule types, we assembled and capsule typed an international collection of K. kingae isolates. The findings reported here show that the type a and type b capsules represent >95% of the invasive isolates, similar to the Israeli isolate collection, suggesting that a polysaccharide-based vaccine targeting these two capsules could be an attractive approach to prevent K. kingae disease.
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Additional file 1: Figure S1. of A modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Dimitri Ceroni, Pablo Yagupsky, Jean-christophe Pons, Guillaume Durand, Anis Oubraham, Didier RaoultAbstract:MAFFT alignment of MLST genomic regions of the abcZ, adk, aroE, cpn60, gdh/zwf, recA genes from the 40 Kingella kingae strains that were used in this study, and those from 6 closely related Kingella and Neisseria species. Only each distinct variant of K. kingae sequence types is represented. MAFFT alignment and figures were performed by using Geneious 10.2.3 (Biomatters). (PPTX 24674 kb
Romain Basmaci - One of the best experts on this subject based on the ideXlab platform.
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Temporal association between rhinovirus activity and Kingella kingae osteoarticular infections
Journal of Pediatrics, 2018Co-Authors: Nina Droz, Stephane Bonacorsi, Vincent Enouf, Damir Mohamed, Sylvie Behillil, Anne-laure Simon, Manon Bachy, Marion Caseris, Romain BasmaciAbstract:OBJECTIVE: To determine whether the seasonal distribution of Kingella kingae osteoarticular infections is similar to that of common respiratory viruses. STUDY DESIGN: Between October 2009 and September 2016, we extracted the results of K kingae-specific real-time polymerase chain reaction analyses performed for bone or joint specimens in patients from 2 pediatric tertiary care centers in Paris. We used data of respiratory virus detection from the Réseau National des Laboratoires network with coordination with the National Influenza Center of France. The Spearman rank correlation was used to assess a correlation between weekly distributions, with P
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Kingella kingae from carriage to infection
Canadian Medical Association Journal, 2017Co-Authors: Romain Basmaci, Stephane BonacorsiAbstract:[See related article at [www.cmaj.ca/lookup/doi/10.1503/cmaj.170127][2]][2] KEY POINTS In a linked research paper,[1][2] Gravel and colleagues evaluated the association between carriage of Kingella kingae in the oropharynx of preschool children and osteoarticular infections in a prospective case–
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genome analysis of Kingella kingae strain kwg1 reveals how a β lactamase gene inserted in the chromosome of this species
Antimicrobial Agents and Chemotherapy, 2016Co-Authors: C. Doit, Romain Basmaci, Christelle Jost, Julien GuglielminiAbstract:We describe the genome of a penicillinase-producing Kingella kingae strain (KWG1), the first to be isolated in continental Europe, whose bla(TEM-1) gene was, for the first time in this species, found to be chromosomally inserted. The bla(TEM) gene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids of K. kingae and other Gram-negative bacteria.
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penicillinase encoding gene blatem 1 may be plasmid borne or chromosomally located in Kingella kingae species
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Romain Basmaci, Stephane Bonacorsi, Pablo Yagupsky, Christelle JostAbstract:Kingella kingae is the prime pathogen of osteoarticular infections in young children in numerous countries ([1][1][–][2][4][3]). Although K. kingae is usually susceptible to antibiotics that are administered to children with skeletal system infections, β-lactamase production has been sporadically
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high respiratory virus oropharyngeal carriage rate during Kingella kingae osteoarticular infections in children
Future Microbiology, 2015Co-Authors: Romain Basmaci, C. Doit, M. Lorrot, Brice Ilharreborde, Stephane Bonacorsi, Mahmoud Kahil, Benoit Visseaux, Nadhira HouhouAbstract:ABSTRACT Aim: Kingella kingae osteoarticular (KKO) infections are frequently associated with upper respiratory tract infections. However, no comparative studies detecting respiratory viruses had ever been performed between KKO and non-KKO (NKO). Patients & methods: Eighteen viruses were searched by FilmArray® Respiratory Panel (BioFire Diagnostics, UT, USA) in the oropharynx of 6-to-48-month-children admitted for KKO and NKO in 2013. Results: At least one virus was detected in the oropharynx of 19/21 (90.5%) KKO and 3/8 (37.5%) NKO cases (p = 0.008). In KKO group, human rhinovirus was predominant (12/21; 57.1%), especially during winter (7/11; 63.6%) despite its low concomitant circulation (<10%). Human rhinovirus was found in 2/8 (25%) in NKO group. Conclusion: Higher prevalence of respiratory virus in oropharynx was observed in KKO than NKO, strengthening their putative role in KKO pathophysiology.
Stephane Bonacorsi - One of the best experts on this subject based on the ideXlab platform.
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Temporal association between rhinovirus activity and Kingella kingae osteoarticular infections
Journal of Pediatrics, 2018Co-Authors: Nina Droz, Stephane Bonacorsi, Vincent Enouf, Damir Mohamed, Sylvie Behillil, Anne-laure Simon, Manon Bachy, Marion Caseris, Romain BasmaciAbstract:OBJECTIVE: To determine whether the seasonal distribution of Kingella kingae osteoarticular infections is similar to that of common respiratory viruses. STUDY DESIGN: Between October 2009 and September 2016, we extracted the results of K kingae-specific real-time polymerase chain reaction analyses performed for bone or joint specimens in patients from 2 pediatric tertiary care centers in Paris. We used data of respiratory virus detection from the Réseau National des Laboratoires network with coordination with the National Influenza Center of France. The Spearman rank correlation was used to assess a correlation between weekly distributions, with P
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Kingella kingae from carriage to infection
Canadian Medical Association Journal, 2017Co-Authors: Romain Basmaci, Stephane BonacorsiAbstract:[See related article at [www.cmaj.ca/lookup/doi/10.1503/cmaj.170127][2]][2] KEY POINTS In a linked research paper,[1][2] Gravel and colleagues evaluated the association between carriage of Kingella kingae in the oropharynx of preschool children and osteoarticular infections in a prospective case–
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penicillinase encoding gene blatem 1 may be plasmid borne or chromosomally located in Kingella kingae species
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Romain Basmaci, Stephane Bonacorsi, Pablo Yagupsky, Christelle JostAbstract:Kingella kingae is the prime pathogen of osteoarticular infections in young children in numerous countries ([1][1][–][2][4][3]). Although K. kingae is usually susceptible to antibiotics that are administered to children with skeletal system infections, β-lactamase production has been sporadically
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high respiratory virus oropharyngeal carriage rate during Kingella kingae osteoarticular infections in children
Future Microbiology, 2015Co-Authors: Romain Basmaci, C. Doit, M. Lorrot, Brice Ilharreborde, Stephane Bonacorsi, Mahmoud Kahil, Benoit Visseaux, Nadhira HouhouAbstract:ABSTRACT Aim: Kingella kingae osteoarticular (KKO) infections are frequently associated with upper respiratory tract infections. However, no comparative studies detecting respiratory viruses had ever been performed between KKO and non-KKO (NKO). Patients & methods: Eighteen viruses were searched by FilmArray® Respiratory Panel (BioFire Diagnostics, UT, USA) in the oropharynx of 6-to-48-month-children admitted for KKO and NKO in 2013. Results: At least one virus was detected in the oropharynx of 19/21 (90.5%) KKO and 3/8 (37.5%) NKO cases (p = 0.008). In KKO group, human rhinovirus was predominant (12/21; 57.1%), especially during winter (7/11; 63.6%) despite its low concomitant circulation (<10%). Human rhinovirus was found in 2/8 (25%) in NKO group. Conclusion: Higher prevalence of respiratory virus in oropharynx was observed in KKO than NKO, strengthening their putative role in KKO pathophysiology.
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unusually severe cases of Kingella kingae osteoarticular infections in children
Pediatric Infectious Disease Journal, 2014Co-Authors: Cindy Mallet, M. Lorrot, K. Mazda, Stephane Bonacorsi, Dimitri Ceroni, Estelle Litzelmann, Victor Duboisferriere, Brice IlharrebordeAbstract:With the development of molecular biology and specific polymerase chain reaction, Kingella kingae has become the primary diagnosis of osteoarticular infections in young children. Clinical features of these osteoarticular infections are typically mild, and outcome is almost always favorable. We report a series of unusually severe cases of K. kingae osteoarticular infections.
Dimitri Ceroni - One of the best experts on this subject based on the ideXlab platform.
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primary pyomyositis caused by Kingella kingae in a 21 month old infant a case report
Pediatric Infectious Disease Journal, 2021Co-Authors: Moez Chargui, Romain Dayer, Celine Habre, Christina Steiger, Giacomo De Marco, Benoit Borner, Dimitri CeroniAbstract:ABSTRACT The authors report a rare case of an unusual primary pyomyositis of the biceps cruralis assigned to Kingella kingae in a 21-month-old girl. The reported case demonstrated that primary pyomyositis may be encountered during invasive infection due to K. kingae even if this manifestation remains rare. This bacterial etiology must, therefore, be evoked when a primary pyomyositis is observed, and this is in particular in children under 4 years of age.
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Fulminant Infective Endocarditis Due to Kingella kingae and Several Complications in a 6-Year-Old Girl: A Case Report
'Frontiers Media SA', 2021Co-Authors: Raphael Joye, Dimitri Ceroni, Maurice Beghetti, Yacine Aggoun, Tornike SologashviliAbstract:Kingella kingae is a gram-negative coccobacillus belonging to the HACEK group (Haemophilus species, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella species) and is a common oropharyngeal colonizer of healthy young children. Osteoarticular infection is the most commonly reported invasive Kingella kingae infection in children, usually presenting a mild clinical picture. However, it can also cause severe invasive infections, especially infective endocarditis, with a high complication rate. We report the case of a 6-year-old girl, with no past medical history, who presented with fulminant infective endocarditis due to Kingella kingae. She received emergency venoarterial extracorporeal membrane oxygenation support, rapidly underwent cardiac surgery, and was then treated using ceftriaxone for 4 weeks as recommended by the American Heart Association. The patient's postoperative course was marked by a cerebral ischemic stroke consistent with septic embolism. She also presented with a para-aortic pseudoaneurysm that required a secondary surgical procedure, with a good postoperative result. This report illustrates a case of fulminant infective endocarditis due to Kingella kingae and responsible for two major complications. We also describe the preventive valve surgery performed to ensure the preservation of valve function and its capacity for growth
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osteoarticular infections of the chest wall due to Kingella kingae a series of 8 cases
Pediatric Infectious Disease Journal, 2020Co-Authors: Dimitri Ceroni, Romain Dayer, Giacomo Demarco, Benoit Coulin, Tanguy Vendeuvre, Vanessa Morello, Celine Habre, Christina SteigerAbstract:Osteoarticular infections of the chest wall are relatively uncommon in pediatric patients and affect primarily infants and toddlers. Clinical presentation is often vague and nonspecific. Laboratory findings may be unremarkable in osteoarticular chest wall infections and not suggestive of an osteoarticular infection. Causative microbes are frequently identified if specific nucleic acid amplification assays are carried out. In the young pediatric population, there is evidence that Kingella kingae is 1 of the main the main causative pathogens of osteoarticular infections of the chest wall.
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Kingella kingae and osteoarticular infections
Pediatrics, 2019Co-Authors: Eleftheria Samara, Sergio Manzano, Romain Dayer, Vasiliki Spyropoulou, Anne Tabardfougere, Laura Merlini, Raimonda Valaikaite, Amira Dhouib, Celine Juchler, Dimitri CeroniAbstract:OBJECTIVES: In this study, we aimed to contrast the bacteriologic epidemiology of osteoarticular infections (OAIs) between 2 patient groups in successive 10-year periods, before and after the extensive use of nucleic acid amplification assays in the diagnostic process. METHODS: Epidemiologic data and bacteriologic etiologies of all children presenting with OAIs on admission to our institution over 20 years (1997–2016) were assessed retrospectively. The population was divided into 2 cohorts, using the standardized use of polymerase chain reaction as the cutoff point (2007). The conventional cohort included children with OAIs mainly investigated by using classic cultures, whereas the molecular cohort referred to patients also investigated by using molecular assays. RESULTS: Kingella kingae was the most frequently isolated pathogen, responsible for 51% of OAIs, whereas other classic pathogens were responsible for 39.7% of cases in the molecular cohort. A statistically significant increase in the mean incidence of OAIs was observed, as was a decrease in the mean age at diagnosis after 2007. After 2007, the pathogen remained unidentified in 21.6% of OAIs in our pediatric population. CONCLUSIONS: Extensive use of nucleic acid amplification assays improved the detection of fastidious pathogens and has increased the observed incidence of OAI, especially in children aged between 6 and 48 months. We propose the incorporation of polymerase chain reaction assays into modern diagnostic algorithms for OAIs to better identify the bacteriologic etiology of OAIs.
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Additional file 1: Figure S1. of A modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Dimitri Ceroni, Pablo Yagupsky, Jean-christophe Pons, Guillaume Durand, Anis Oubraham, Didier RaoultAbstract:MAFFT alignment of MLST genomic regions of the abcZ, adk, aroE, cpn60, gdh/zwf, recA genes from the 40 Kingella kingae strains that were used in this study, and those from 6 closely related Kingella and Neisseria species. Only each distinct variant of K. kingae sequence types is represented. MAFFT alignment and figures were performed by using Geneious 10.2.3 (Biomatters). (PPTX 24674 kb
Jacques Schrenzel - One of the best experts on this subject based on the ideXlab platform.
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Comparing the oropharyngeal colonization density of Kingella kingae between asymptomatic carriers and children with invasive osteoarticular infections.
The Pediatric infectious disease journal, 2013Co-Authors: Dimitri Ceroni, Abdessalam Cherkaoui, Rebecca Anderson Della Llana, Omar Kherad, Victor Dubois-ferriere, Pierre Lascombes, Gesuele Renzi, Léopold Lamah, Sergio Manzano, Jacques SchrenzelAbstract:Colonization of the oropharynx by Kingella kingae is currently considered to be a prerequisite for later development of invasive infections. However, the oropharyngeal K. kingae DNA bacterial load in children with osteoarticular infections caused by this microorganism is not different than that of asymptomatic carriers.
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30 years of study of Kingella kingae: post tenebras, lux.
Future microbiology, 2013Co-Authors: Dimitri Ceroni, Abdessalam Cherkaoui, Victor Dubois-ferriere, Pierre Lascombes, Gesuele Renzi, Léopold Lamah, Belaieff Wilson, Jacques SchrenzelAbstract:Kingella kingae is a Gram-negative bacterium that is today recognized as the major cause of joint and bone infections in young children. This microorganism is a member of the normal flora of the oropharynx, and the carriage rate among children under 4 years of age is approximately 10%. K. kingae is transmitted from child to child through close personal contact. Key virulence factors of K. kingae include expression of type IV pili, Knh-mediated adhesive activity and production of a potent RTX toxin. The clinical presentation of K. kingae invasive infection is often subtle and may be associated to mild-to-moderate biologic inflammatory responses, highlighting the importance a high index of suspicion. Molecular diagnosis of K. kingae infections by nucleic acid amplification techniques enables identification of this fastidious microorganism. Invasive infections typically respond favorably to medical treatment, with the exception of cases of endocarditis, which may require urgent valve replacement.
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small risk of osteoarticular infections in children with asymptomatic oropharyngeal carriage of Kingella kingae
Pediatric Infectious Disease Journal, 2012Co-Authors: Dimitri Ceroni, Abdessalam Cherkaoui, Léopold Lamah, Victor Duboisferriere, Christophe Combescure, Rebecca Anderson, Jacques SchrenzelAbstract:The aim of this study was to evaluate the absolute risk for children younger than 4 years of age with asymptomatic oropharyngeal carriage of Kingella kingae to sustain an osteoarticular infection. The rate of K. kingae carriage in the oropharyngeal mucosa was 9% among healthy children, and the risk for an asymptomatic carrier to develop an osteoarticular infection due to K. kingae was estimated to be lower than 1%.
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differentiating osteoarticular infections caused by Kingella kingae from those due to typical pathogens in young children
Pediatric Infectious Disease Journal, 2011Co-Authors: Dimitri Ceroni, Abdessalam Cherkaoui, Christophe Combescure, Patrice Francois, Andre Kaelin, Jacques SchrenzelAbstract:Osteoarticular infections caused by Kingella kingae are characterized by mild-to-moderate clinical and biologic inflammatory signs that are different from those caused by Gram-positive cocci. A combined score was built to find the best model to predict K. kingae osteoarticular infections by using the following 4 variables: body temperature <38°C, serum C-reactive protein <55 mg/L, white blood cell count <14,000/mm, and band forms <150/mm.
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Kingella kingae spondylodiscitis in young children: toward a new approach for bacteriological investigations? A preliminary report
Journal of Children's Orthopaedics, 2010Co-Authors: Dimitri Ceroni, Abdessalam Cherkaoui, Andre Kaelin, Jacques SchrenzelAbstract:As the result of improved bacteriological techniques, Kingella kingae is a slow-growing Gram-negative coccobacillus that is emerging as an important cause of spondylodiscitis in children younger than 3 years of age. The high pharyngeal carrier rates of this slow-growing Gram-negative coccobacillus combined with the low incidence of identified K. kingae infections is possibly explained by a low virulence of this bacterium. The use of specific real-time polymerase chain reaction (PCR) on blood samples and throat swabs opens new prospects in the bacteriological investigations of young children suspected to have spondylodiscitis, an approach that could prevent, in the future, unnecessary invasive interventions.
