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Pablo Yagupsky - One of the best experts on this subject based on the ideXlab platform.
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Kingella negevensis shares multiple putative virulence factors with Kingella kingae
PLOS ONE, 2020Co-Authors: Eric A Porsch, Joseph W St Geme, Pablo YagupskyAbstract:Kingella negevensis is a newly described gram-negative bacterium in the Neisseriaceae family and is closely related to Kingella kingae, an important cause of pediatric osteoarticular infections and other invasive diseases. Like K. kingae, K. negevensis can be isolated from the oropharynx of young children, although at a much lower rate. Due to the potential for misidentification as K. kingae, the burden of disease due to K. negevensis is currently unknown. Similarly, there is little known about virulence factors present in K. negevensis and how they compare to virulence factors in K. kingae. Using a variety of approaches, we show that K. negevensis produces many of the same putative virulence factors that are present in K. kingae, including a polysaccharide capsule, a secreted exopolysaccharide, a Knh-like trimeric autotransporter, and type IV pili, suggesting that K. negevensis may have significant pathogenic potential.
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on king saul two missing mules and Kingella kingae the serendipitous discovery of a pediatric pathogen
Pediatric Infectious Disease Journal, 2018Co-Authors: Pablo Yagupsky, Ron DaganAbstract:For the first 2 decades following Kingella kingae's initial characterization, this fastidious organism was considered an unusual cause of human infection until a study published in 1992 reported that inoculation of synovial fluid aspirates into blood culture vials improved the recovery of the bacterium. The authors of the original publication report herein the history of the discovery and review the progress made in the research of the organism.
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respiratory carriage of the novel Kingella negevensis species by young children
new microbes and new infections, 2018Co-Authors: Pablo Yagupsky, El N. Houmami, Pierre-edouard FournierAbstract:Kingella negevensis, a novel Kingella species implicated in a pediatric joint infection, has been recently characterized but its epidemiology remains largely unknown. The pharyngeal carriage of K. negevensis was studied by re-examining the results of a previous longitudinal study conducted in a cohort of healthy Israeli children from whom upper respiratory tract specimens were sequentially cultured between the ages of 2 and 36 months. Isolates were identified as K. negevensis by a species-specific nucleic amplification assay and genotyped by pulsed-field gel electrophoresis. β-lactamase production was determined by the nitrocephin test. Kingella negevensis was detected in 26 of 4,472 (0.58%) oropharyngeal cultures obtained from 24 of 716 children (3.35%) and was not isolated from any of 4,472 nasopharyngeal specimens. Following the first 6 months of life during which none of the children was colonized, the prevalence of carriage gradually increased reaching a peak of 1.09% at 24 months of age and decreased thereafter. Kingella negevensis strains showed genomic heterogeneity, and two clones represented 22 of 26 (84.62%) isolates. Twelve of the 26 (46.15%) isolates, belonging to two distinct clones, produced β-lactamase. Kingella negevensis shows remarkable similarities with K. kingae in terms of colonization site, age-related patterns of acquisition and carriage, and clonal distribution of β-lactamase production. Additional research is needed to investigate potential colonization sites of K. negevensis outside the respiratory tract, explore the mechanisms of pharyngeal colonization by the organism, and determine its role as an invasive human pathogen.
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a modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
BMC Microbiology, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Guillaume Andre Durand, Pablo Yagupsky, Dimitri Ceroni, Didier Raoult, Jean-christophe Pons, Anis Oubraham, Philippe BidetAbstract:Outbreaks of Kingella kingae infection are an emerging public health concern among daycare attendees carrying epidemic clones in the oropharynx. However, genotyping of such epidemic clones from affected cases is limited by the low performance of current methods to detect K. kingae from blood samples and lack of specimens available from infected sites. We aimed at developing a modified multilocus sequence typing (MLST) method to genotype K. kingae strains from oropharyngeal samples without prior culture. We designed in silico MLST primers specific for K. kingae by aligning whole nucleotide sequences of abcZ, adk, aroE, cpn60, recA, and gdh/zwf genes from closely related species belonging to the Kingella and Neisseria genera. We tested our modified MLST protocol on all Kingella species and N. meningitidis, as well as 11 oropharyngeal samples from young children with sporadic (n = 10) or epidemic (n = 1) K. kingae infection. We detected K. kingae-specific amplicons in the 11 oropharyngeal samples, corresponding to sequence-type 6 (ST-6) in 6 children including the epidemic cases, ST-25 in 2 children, and 3 possible novel STs (ST-67, ST-68, and ST-69). No amplicon was obtained from other Kingella species and N. meningitidis. We herein developed a specific MLST protocol that enables genotyping of K. kingae by MLST directly from oropharyngeal samples. This discriminatory tool, with which we identified the first K. kingae outbreak caused by ST-6 in Europe, may be used in further epidemiological investigations.
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draft genome sequence of Kingella negevensis sw7208426 the first european strain of k negevensis isolated from a healthy child in switzerland
Genome Announcements, 2017Co-Authors: Nawal El Houmami, Catherine Robert, Pablo Yagupsky, Dimitri Ceroni, Jacques Schrenzel, Didier Raoult, Pierre-edouard FournierAbstract:We report here the draft genome of Kingella negevensis strain SW7208426, isolated from the oropharynx of a healthy 6-year-old boy in Geneva, Switzerland. To our knowledge, this is the first genome report of the newly described K. negevensis species from Europe.
Stephane Bonacorsi - One of the best experts on this subject based on the ideXlab platform.
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molecular tests that target the rtx locus do not distinguish between Kingella kingae and the recently described Kingella negevensis species
Journal of Clinical Microbiology, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Elsa Prudent, Guillaume Andre Durand, Sofiane Bakour, Herve Seligmann, Stephane BonacorsiAbstract:ABSTRACT Kingella kingae is an important invasive pathogen in early childhood. The organism elaborates an RTX toxin presumably restricted to this species. Consequently, real-time quantitative PCR (qPCR) assays targeting the RTX locus have been developed in recent years and are gaining increasing use for the molecular diagnosis of K. kingae infections. However, the present study shows that Kingella negevensis, a Kingella species newly identified in young children, harbors an identical Kingella RTX locus, raising the question of whether K. negevensis can be misidentified as K. kingae by clinical microbiology laboratories. In silico comparison of Kingella sp. RTX and groEL genes and in vitro studies provided evidence that targeting the rtxA and rtxB genes could not differentiate between strains of K. kingae and K. negevensis, whereas targeting the groEL gene could. This prompted the design of a highly specific and sensitive qPCR assay targeting K. negevensis groEL (kngroEL). Ninety-nine culture-negative osteoarticular specimens from 99 children younger than 4 years of age were tested with a conventional 16S rRNA gene-based broad-range PCR assay and Kingella-specific rtxB, K. kingae-specific groEL (kkgroEL), and kngroEL qPCR assays. Forty-two specimens were rtxB positive, including 41 that were also kkgroEL positive and 1 (the remaining one) that was kngroEL positive. Thus, this study discloses an invasive infection caused by K. negevensis in humans and demonstrates that targeting the RTX locus cannot be used for the formal diagnosis of K. kingae infections. These findings stress the need for further studies on the epidemiology of asymptomatic carriage and invasive infections caused by K. negevensis in humans.
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patterns of Kingella kingae disease outbreaks
Pediatric Infectious Disease Journal, 2016Co-Authors: Nawal El Houmami, P Minodier, Jean-luc Jouve, Stephane Bonacorsi, Gregory Dubourg, Pablo Yagupsky, Romain Basmaci, Audrey Mirand, Remi N Charrel, Didier RaoultAbstract:Background:Kingella kingae outbreaks occur sporadically in childcare centers but remain poorly understood and difficult to identify.Methods:To provide the basis of a better knowledge of K. kingae outbreaks patterns that may help to guide identification and management strategies, we collected epidemi
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penicillinase encoding gene blatem 1 may be plasmid borne or chromosomally located in Kingella kingae species
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Stephane Bonacorsi, Pablo Yagupsky, Romain Basmaci, Philippe Bidet, Christelle JostAbstract:Kingella kingae is the prime pathogen of osteoarticular infections in young children in numerous countries ([1][1][–][2][4][3]). Although K. kingae is usually susceptible to antibiotics that are administered to children with skeletal system infections, β-lactamase production has been sporadically
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unusually severe cases of Kingella kingae osteoarticular infections in children
Pediatric Infectious Disease Journal, 2014Co-Authors: Cindy Mallet, Stephane Bonacorsi, M. Lorrot, Keyvan Mazda, Dimitri Ceroni, Estelle Litzelmann, Victor Duboisferriere, Brice IlharrebordeAbstract:With the development of molecular biology and specific polymerase chain reaction, Kingella kingae has become the primary diagnosis of osteoarticular infections in young children. Clinical features of these osteoarticular infections are typically mild, and outcome is almost always favorable. We report a series of unusually severe cases of K. kingae osteoarticular infections.
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Two Atypical Cases of Kingella kingae Invasive Infection with Concomitant Human Rhinovirus Infection
Journal of clinical microbiology, 2013Co-Authors: Romain Basmaci, Brice Ilharreborde, Catherine Doit, Ana Presedo, M. Lorrot, Marianne Alison, Keyvan Mazda, Philippe Bidet, Stephane BonacorsiAbstract:We describe two atypical cases of Kingella kingae infection in children diagnosed by PCR, one case involving a soft tissue abscess and one case a femoral Brodie abscess. Both patients had concomitant human rhinovirus infection. K. kingae strains, isolated from an oropharyngeal swab, were characterized by multilocus sequence typing and rtxA sequencing.
Philippe Bidet - One of the best experts on this subject based on the ideXlab platform.
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a modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
BMC Microbiology, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Guillaume Andre Durand, Pablo Yagupsky, Dimitri Ceroni, Didier Raoult, Jean-christophe Pons, Anis Oubraham, Philippe BidetAbstract:Outbreaks of Kingella kingae infection are an emerging public health concern among daycare attendees carrying epidemic clones in the oropharynx. However, genotyping of such epidemic clones from affected cases is limited by the low performance of current methods to detect K. kingae from blood samples and lack of specimens available from infected sites. We aimed at developing a modified multilocus sequence typing (MLST) method to genotype K. kingae strains from oropharyngeal samples without prior culture. We designed in silico MLST primers specific for K. kingae by aligning whole nucleotide sequences of abcZ, adk, aroE, cpn60, recA, and gdh/zwf genes from closely related species belonging to the Kingella and Neisseria genera. We tested our modified MLST protocol on all Kingella species and N. meningitidis, as well as 11 oropharyngeal samples from young children with sporadic (n = 10) or epidemic (n = 1) K. kingae infection. We detected K. kingae-specific amplicons in the 11 oropharyngeal samples, corresponding to sequence-type 6 (ST-6) in 6 children including the epidemic cases, ST-25 in 2 children, and 3 possible novel STs (ST-67, ST-68, and ST-69). No amplicon was obtained from other Kingella species and N. meningitidis. We herein developed a specific MLST protocol that enables genotyping of K. kingae by MLST directly from oropharyngeal samples. This discriminatory tool, with which we identified the first K. kingae outbreak caused by ST-6 in Europe, may be used in further epidemiological investigations.
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Additional file 1: Figure S1. of A modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Pablo Yagupsky, Dimitri Ceroni, Didier Raoult, Jean-christophe Pons, Guillaume Durand, Anis Oubraham, Philippe BidetAbstract:MAFFT alignment of MLST genomic regions of the abcZ, adk, aroE, cpn60, gdh/zwf, recA genes from the 40 Kingella kingae strains that were used in this study, and those from 6 closely related Kingella and Neisseria species. Only each distinct variant of K. kingae sequence types is represented. MAFFT alignment and figures were performed by using Geneious 10.2.3 (Biomatters). (PPTX 24674 kb
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genome analysis of Kingella kingae strain kwg1 reveals how a β lactamase gene inserted in the chromosome of this species
Antimicrobial Agents and Chemotherapy, 2016Co-Authors: Romain Basmaci, Catherine Doit, Philippe Bidet, Christelle Jost, Julien GuglielminiAbstract:We describe the genome of a penicillinase-producing Kingella kingae strain (KWG1), the first to be isolated in continental Europe, whose bla(TEM-1) gene was, for the first time in this species, found to be chromosomally inserted. The bla(TEM) gene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids of K. kingae and other Gram-negative bacteria.
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penicillinase encoding gene blatem 1 may be plasmid borne or chromosomally located in Kingella kingae species
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Stephane Bonacorsi, Pablo Yagupsky, Romain Basmaci, Philippe Bidet, Christelle JostAbstract:Kingella kingae is the prime pathogen of osteoarticular infections in young children in numerous countries ([1][1][–][2][4][3]). Although K. kingae is usually susceptible to antibiotics that are administered to children with skeletal system infections, β-lactamase production has been sporadically
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first identification of a chromosomally located penicillinase gene in Kingella kingae species isolated in continental europe
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: Romain Basmaci, Philippe Bidet, Beatrice Bercot, Christelle Jost, Theresa Kwon, Elodie GaumetouAbstract:Kingella kingae is the major pathogen causing osteoarticular infections (OAI) in young children in numerous countries. Plasmid-borne TEM-1 penicillinase production has been sporadically detected in a few countries but not in continental Europe, despite a high prevalence of K. kingae infections. We describe here for the first time a K. kingae β-lactamase-producing strain in continental Europe and demonstrate the novel chromosomal location of the blaTEM-1 gene in K. kingae species.
Nawal El Houmami - One of the best experts on this subject based on the ideXlab platform.
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A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens
Journal of Clinical Microbiology, 2018Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Guillaume Andre Durand, Herve Seligmann, Didier Raoult, Anne Darmon, Pierre-edouard FournierAbstract:Kingella kingae is a significant pediatric pathogen responsible for bone and joint infections, occult bacteremia, and endocarditis in early childhood. Past efforts to detect this bacterium using culture and broad-range 16S rRNA gene PCR assays from clinical specimens have proven unsatisfactory; therefore, by the late 2000s, these were gradually phased out to explore the benefits of specific real-time PCR tests targeting the groEL gene and the RTX locus of K. kingae. However, recent studies showed that real-time PCR (RT-PCR) assays targeting the Kingella sp. RTX locus that are currently available for the diagnosis of K. kingae infection lack specificity because they could not distinguish between K. kingae and the recently described Kingella negevensis species. Furthermore, in silico analysis of the groEL gene from a large collection of 45 K. kingae strains showed that primers and probes from K. kingae groEL-based RT-PCR assays display a few mismatches with K. kingae groEL variations that may result in decreased detection sensitivity, especially in paucibacillary clinical specimens. In order to provide an alternative to groEL-and RTX-targeting RTPCR assays that may suffer from suboptimal specificity and sensitivity, a K. kingae-specific RT-PCR assay targeting the malate dehydrogenase (mdh) gene was developed for predicting no mismatch between primers and probe and 18 variants of the K. kingae mdh gene from 20 distinct sequence types of K. kingae. This novel K. kingae-specific RT-PCR assay demonstrated high specificity and sensitivity and was successfully used to diagnose K. kingae infections and carriage in 104 clinical specimens from children between 7 months and 7 years old.
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molecular tests that target the rtx locus do not distinguish between Kingella kingae and the recently described Kingella negevensis species
Journal of Clinical Microbiology, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Elsa Prudent, Guillaume Andre Durand, Sofiane Bakour, Herve Seligmann, Stephane BonacorsiAbstract:ABSTRACT Kingella kingae is an important invasive pathogen in early childhood. The organism elaborates an RTX toxin presumably restricted to this species. Consequently, real-time quantitative PCR (qPCR) assays targeting the RTX locus have been developed in recent years and are gaining increasing use for the molecular diagnosis of K. kingae infections. However, the present study shows that Kingella negevensis, a Kingella species newly identified in young children, harbors an identical Kingella RTX locus, raising the question of whether K. negevensis can be misidentified as K. kingae by clinical microbiology laboratories. In silico comparison of Kingella sp. RTX and groEL genes and in vitro studies provided evidence that targeting the rtxA and rtxB genes could not differentiate between strains of K. kingae and K. negevensis, whereas targeting the groEL gene could. This prompted the design of a highly specific and sensitive qPCR assay targeting K. negevensis groEL (kngroEL). Ninety-nine culture-negative osteoarticular specimens from 99 children younger than 4 years of age were tested with a conventional 16S rRNA gene-based broad-range PCR assay and Kingella-specific rtxB, K. kingae-specific groEL (kkgroEL), and kngroEL qPCR assays. Forty-two specimens were rtxB positive, including 41 that were also kkgroEL positive and 1 (the remaining one) that was kngroEL positive. Thus, this study discloses an invasive infection caused by K. negevensis in humans and demonstrates that targeting the RTX locus cannot be used for the formal diagnosis of K. kingae infections. These findings stress the need for further studies on the epidemiology of asymptomatic carriage and invasive infections caused by K. negevensis in humans.
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a modified multilocus sequence typing protocol to genotype Kingella kingae from oropharyngeal swabs without bacterial isolation
BMC Microbiology, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Guillaume Andre Durand, Pablo Yagupsky, Dimitri Ceroni, Didier Raoult, Jean-christophe Pons, Anis Oubraham, Philippe BidetAbstract:Outbreaks of Kingella kingae infection are an emerging public health concern among daycare attendees carrying epidemic clones in the oropharynx. However, genotyping of such epidemic clones from affected cases is limited by the low performance of current methods to detect K. kingae from blood samples and lack of specimens available from infected sites. We aimed at developing a modified multilocus sequence typing (MLST) method to genotype K. kingae strains from oropharyngeal samples without prior culture. We designed in silico MLST primers specific for K. kingae by aligning whole nucleotide sequences of abcZ, adk, aroE, cpn60, recA, and gdh/zwf genes from closely related species belonging to the Kingella and Neisseria genera. We tested our modified MLST protocol on all Kingella species and N. meningitidis, as well as 11 oropharyngeal samples from young children with sporadic (n = 10) or epidemic (n = 1) K. kingae infection. We detected K. kingae-specific amplicons in the 11 oropharyngeal samples, corresponding to sequence-type 6 (ST-6) in 6 children including the epidemic cases, ST-25 in 2 children, and 3 possible novel STs (ST-67, ST-68, and ST-69). No amplicon was obtained from other Kingella species and N. meningitidis. We herein developed a specific MLST protocol that enables genotyping of K. kingae by MLST directly from oropharyngeal samples. This discriminatory tool, with which we identified the first K. kingae outbreak caused by ST-6 in Europe, may be used in further epidemiological investigations.
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draft genome sequence of Kingella negevensis sw7208426 the first european strain of k negevensis isolated from a healthy child in switzerland
Genome Announcements, 2017Co-Authors: Nawal El Houmami, Catherine Robert, Pablo Yagupsky, Dimitri Ceroni, Jacques Schrenzel, Didier Raoult, Pierre-edouard FournierAbstract:We report here the draft genome of Kingella negevensis strain SW7208426, isolated from the oropharynx of a healthy 6-year-old boy in Geneva, Switzerland. To our knowledge, this is the first genome report of the newly described K. negevensis species from Europe.
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an outbreak of Kingella kingae infections complicating a severe hand foot and mouth disease outbreak in nice france 2016
Pediatric Infectious Disease Journal, 2017Co-Authors: Nawal El Houmami, Janek Bzdrenga, P Minodier, Sofiane Bakour, Virginie Cointat, Audrey Mirand, Virginie Fouilloux, Marieamelie Dubois, Francoise Anavefrapech, Remi N CharrelAbstract:We report the investigation methods for the diagnosis of an epidemic and culture-negative Kingella kingae endocarditis complicating a severe outbreak of hand, foot and mouth disease in a childcare center. The diagnosis was confirmed by polymerase chain reaction testing performed from cardiac tissue.
Romain Basmaci - One of the best experts on this subject based on the ideXlab platform.
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patterns of Kingella kingae disease outbreaks
Pediatric Infectious Disease Journal, 2016Co-Authors: Nawal El Houmami, P Minodier, Jean-luc Jouve, Stephane Bonacorsi, Gregory Dubourg, Pablo Yagupsky, Romain Basmaci, Audrey Mirand, Remi N Charrel, Didier RaoultAbstract:Background:Kingella kingae outbreaks occur sporadically in childcare centers but remain poorly understood and difficult to identify.Methods:To provide the basis of a better knowledge of K. kingae outbreaks patterns that may help to guide identification and management strategies, we collected epidemi
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genome analysis of Kingella kingae strain kwg1 reveals how a β lactamase gene inserted in the chromosome of this species
Antimicrobial Agents and Chemotherapy, 2016Co-Authors: Romain Basmaci, Catherine Doit, Philippe Bidet, Christelle Jost, Julien GuglielminiAbstract:We describe the genome of a penicillinase-producing Kingella kingae strain (KWG1), the first to be isolated in continental Europe, whose bla(TEM-1) gene was, for the first time in this species, found to be chromosomally inserted. The bla(TEM) gene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids of K. kingae and other Gram-negative bacteria.
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penicillinase encoding gene blatem 1 may be plasmid borne or chromosomally located in Kingella kingae species
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Stephane Bonacorsi, Pablo Yagupsky, Romain Basmaci, Philippe Bidet, Christelle JostAbstract:Kingella kingae is the prime pathogen of osteoarticular infections in young children in numerous countries ([1][1][–][2][4][3]). Although K. kingae is usually susceptible to antibiotics that are administered to children with skeletal system infections, β-lactamase production has been sporadically
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first identification of a chromosomally located penicillinase gene in Kingella kingae species isolated in continental europe
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: Romain Basmaci, Philippe Bidet, Beatrice Bercot, Christelle Jost, Theresa Kwon, Elodie GaumetouAbstract:Kingella kingae is the major pathogen causing osteoarticular infections (OAI) in young children in numerous countries. Plasmid-borne TEM-1 penicillinase production has been sporadically detected in a few countries but not in continental Europe, despite a high prevalence of K. kingae infections. We describe here for the first time a K. kingae β-lactamase-producing strain in continental Europe and demonstrate the novel chromosomal location of the blaTEM-1 gene in K. kingae species.
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Two Atypical Cases of Kingella kingae Invasive Infection with Concomitant Human Rhinovirus Infection
Journal of clinical microbiology, 2013Co-Authors: Romain Basmaci, Brice Ilharreborde, Catherine Doit, Ana Presedo, M. Lorrot, Marianne Alison, Keyvan Mazda, Philippe Bidet, Stephane BonacorsiAbstract:We describe two atypical cases of Kingella kingae infection in children diagnosed by PCR, one case involving a soft tissue abscess and one case a femoral Brodie abscess. Both patients had concomitant human rhinovirus infection. K. kingae strains, isolated from an oropharyngeal swab, were characterized by multilocus sequence typing and rtxA sequencing.
