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Cletus P. Kurtzman - One of the best experts on this subject based on the ideXlab platform.
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Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees
Antonie van Leeuwenhoek, 2012Co-Authors: Cletus P. KurtzmanAbstract:Two new species of the methanol assimilating ascosporic yeast genus Komagataella are described. Komagataella populi sp. nov. (NRRL YB-455, CBS 12362, type strain, MycoBank accession number = 564110) was isolated from an exudate on a cottonwood tree ( Populus deltoides ), Peoria, Illinois, USA, and Komagataella ulmi sp. nov. (NRRL YB-407, CBS 12361, type strain, MycoBank accession number = 564111) was isolated from the exudate on an elm tree ( Ulmus americana ), also growing in Peoria, Illinois. The species were resolved from divergence in gene sequences for domains D1/D2 LSU rRNA, ITS1-5.8S-ITS2, mitochondrial small subunit rRNA, RNA polymerase subunit 1 and translation elongation factor-1α. Species of Komagataella assimilate few carbon compounds and are unlikely to be resolved from differences in standard growth and fermentation tests. For this reason, separation of species is dependent on gene sequence analysis.
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Komagataella Y. Yamada, Matsuda, Maeda & Mikata (1995)
The Yeasts, 2011Co-Authors: Cletus P. KurtzmanAbstract:Publisher Summary This chapter studies the genus Komagataella. In the determination of asexual reproduction, yeast cells arise by multilateral budding on a narrow base and are spherical to ovoid in shape. Neither pseudohyphae nor true hyphae are produced. In sexual reproduction it is seen that asci may be unconjugated or show conjugation between a cell and its bud or between independent cells. Asci produce one to four hat-shaped ascospores and the spores may be quickly or slowly liberated. Known species are homothallic. In physiology and biochemistry it is seen that glucose is fermented but other sugars are not. This study further examines the phylogenetic placement. The type of species mentioned is Komagataella pastoris. The species accepted are Komagataella pastoris, Komagataella phaffii, and Komagataella pseudopastoris. The systematic discussion of the species includes growth on 5% malt extract agar, growth on the surface of assimilation media, Dalmau plate culture on morphology agar, formation of ascospores, origin of the strains studied, authentic strain, systematics, ecology, biotechnology, agriculture and food, and clinical importance.
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Komagataella y yamada matsuda maeda mikata 1995
The Yeasts (Fifth Edition), 2011Co-Authors: Cletus P. KurtzmanAbstract:Publisher Summary This chapter studies the genus Komagataella. In the determination of asexual reproduction, yeast cells arise by multilateral budding on a narrow base and are spherical to ovoid in shape. Neither pseudohyphae nor true hyphae are produced. In sexual reproduction it is seen that asci may be unconjugated or show conjugation between a cell and its bud or between independent cells. Asci produce one to four hat-shaped ascospores and the spores may be quickly or slowly liberated. Known species are homothallic. In physiology and biochemistry it is seen that glucose is fermented but other sugars are not. This study further examines the phylogenetic placement. The type of species mentioned is Komagataella pastoris. The species accepted are Komagataella pastoris, Komagataella phaffii, and Komagataella pseudopastoris. The systematic discussion of the species includes growth on 5% malt extract agar, growth on the surface of assimilation media, Dalmau plate culture on morphology agar, formation of ascospores, origin of the strains studied, authentic strain, systematics, ecology, biotechnology, agriculture and food, and clinical importance.
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biotechnological strains of Komagataella pichia pastoris are Komagataella phaffii as determined from multigene sequence analysis
Journal of Industrial Microbiology & Biotechnology, 2009Co-Authors: Cletus P. KurtzmanAbstract:Pichiapastoris was reassigned earlier to the genus Komagataella following phylogenetic analysis of gene sequences. Since that time, two additional species of Komagataella have been described, K. pseudopastoris and K. phaffii. Because these three species are unlikely to be resolved from the standard fermentation and growth tests used in yeast taxonomy, the identity of biotechnologically important strains of K. pastoris was determined from multigene sequence analyses. Results from this study show that the strain of ‘Pichia pastoris’ commonly used in gene expression studies is actually K. phaffii.
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Description of Komagataella phaffii sp. nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella.
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Cletus P. KurtzmanAbstract:The new methanol-assimilating yeast species Komagataella phaffii Kurtzman sp. nov. (type strain NRRL Y-7556T=CBS 2612T) is described. Of the four known strains of this species, two were isolated from black oak trees in California, USA, one from an Emory oak in Arizona, USA, and one from an unidentified source in Mexico. The species forms hat-shaped ascospores in deliquescent asci and appears to be homothallic. Analysis of nucleotide sequences from domains D1/D2 of large-subunit (26S) rDNA separates the new species from Komagataella pastoris, the type species of the genus, and from Pichia pseudopastoris, which is here renamed Komagataella pseudopastoris (Dlauchy, Tornai-Lehoczki, Fulop & Peter) Kurtzman comb. nov. (type strain NRRL Y-27603T=CBS 9187T=NCAIM Y 01541T). On the basis of D1/D2 26S rDNA sequence analysis, the three species now assigned to the genus Komagataella represent a clade that is phylogenetically isolated from other ascomycetous yeast genera.
Diethard Mattanovich - One of the best experts on this subject based on the ideXlab platform.
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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family.
FEMS yeast research, 2020Co-Authors: Corinna Rebnegger, Diethard Mattanovich, Nadine E. Tatto, Josef W. Moser, Alexandra B. Graf, Brigitte GasserAbstract:Many yeasts differentiate into multicellular phenotypes in adverse environmental conditions. Here, we investigate pseudohyphal growth in Komagataella phaffii and the involvement of the flocculin (FLO) gene family in its regulation. The K. phaffii FLO family consists of 13 members, and the conditions inducing pseudohyphal growth are different from Saccharomyces cerevisiae. So far, this phenotype was only observed when K. phaffii was cultivated at slow growth rates in glucose-limited chemostats, but not upon nitrogen starvation or the presence of fusel alcohols. Transcriptional analysis identified that FLO11, FLO400 and FLO5-1 are involved in the phenotype, all being controlled by the transcriptional regulator Flo8. The three genes exhibit a complex mechanism of expression and repression during transition from yeast to pseudohyphal form. Unlike in S. cerevisiae, deletion of FLO11 does not completely prevent the phenotype. In contrast, deletion of FLO400 or FLO5-1 prevents pseudohyphae formation, and hampers FLO11 expression. FAIRE-Seq data shows that the expression and repression of FLO400 and FLO5-1 are correlated to open or closed chromatin regions upstream of these genes, respectively. Our findings indicate that K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 upon glucose limitation in chemostats at slow growth and chromatin modulation is involved in the regulation of their expression.
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microbe profile Komagataella phaffii a methanol devouring biotech yeast formerly known as pichia pastoris
Microbiology, 2020Co-Authors: Lina Heistinger, Brigitte Gasser, Diethard MattanovichAbstract:Methylotrophic yeasts of the genus Komagataella are abundantly found in tree exudates. Their ability to utilize methanol as carbon and energy source relies on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as well. Komagataella yeasts usually grow as haploid cells and are secondary homothallic as they can switch mating type. Upon mating diploid cells sporulate readily, forming asci with four haploid spores. Their ability to secrete high amounts of heterologous proteins made them interesting for biotechnology, which expands today also to other products of primary and secondary metabolism.
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Komagataella phaffii YPS1-5 encodes the alpha-factor degrading protease Bar1.
FEMS yeast research, 2020Co-Authors: Lina Heistinger, Brigitte Gasser, Diethard MattanovichAbstract:Yeast mating pheromones are small secreted peptides required for efficient mating between cells of opposite mating type. Pheromone gradients allow the cells to detect potential mating partners. Secreted pheromone degrading proteases steepen local gradients and allow fast recovery from the pheromone signal. The methylotrophic yeast Komagataella phaffii is a preferentially haploid species. Only under nitrogen starvation, mating genes are activated and the cells are able to undergo a full sexual cycle of mating and sporulation. It has been shown that, similar to other yeasts, K. phaffii requires the mating pheromone and pheromone surface receptor genes for efficient mating. The analysis of so far uncharacterized mating-type-specific genes allowed us to identify the K. phaffii α-factor protease gene YPS1-5. It encodes an aspartic protease of the yapsin family and is upregulated only in a-type cells under mating conditions. The phenotype of K. phaffiia-type strains with a deletion in the protease gene was found to be highly similar to the phenotype of Saccharomyces cerevisiae α-factor protease BAR1 deletion strains. They are highly sensitive to α-factor pheromone in pheromone sensitivity assays and were found to mate with reduced efficiency. Based on our results, we propose to rename the gene into K. phaffii BAR1.
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A yeast for all seasons - Is Pichia pastoris a suitable chassis organism for future bioproduction?
FEMS microbiology letters, 2018Co-Authors: Brigitte Gasser, Diethard MattanovichAbstract:The methylotrophic yeast Pichia pastoris (Komagataella spp.) is well established as a host for recombinant protein production. Since a few years it has also been subject to metabolic engineering to produce a diversity of biochemicals. Based on these developments we discuss here why P. pastoris is a suitable future chassis organism for synthetic biology, and we provide a roadmap for a community effort towards this aim.
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Identification and characterization of the Komagataella phaffii mating pheromone genes.
FEMS yeast research, 2018Co-Authors: Lina Heistinger, Brigitte Gasser, Nadine E. Tatto, Minoska Valli, Josef W. Moser, Diethard MattanovichAbstract:The methylotrophic yeast Komagataella phaffii (Pichia pastoris) is a haploid yeast that is able to form diploid cells by mating once nitrogen becomes limiting. Activation of the mating response requires the secretion of a- and α-factor pheromones, which bind to G-protein coupled receptors on cells of opposite mating type. In K. phaffii, the genes coding for the α-factor (MFα), the pheromone surface receptors and the conserved a-factor biogenesis pathway have been annotated previously. Initial homology-based search failed to identify potential a-factor genes (MFA). By using transcriptome data of heterothallic strains under mating conditions, we found two K. phaffiia-factor genes. Deletion of both MFA genes prevented mating of a-type cells. MFA single mutants were still able to mate and activate the mating response pathway in α-type cells. A reporter assay was used to confirm the biological activity of synthetic a- and α-factor peptides. The identification of the a-factor genes enabled the first characterization of the role and regulation of the mating pheromone genes and the response of K. phaffii to synthetic pheromones and will help to gain a better understanding of the mating behavior of K. phaffii.
Brigitte Gasser - One of the best experts on this subject based on the ideXlab platform.
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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family.
FEMS yeast research, 2020Co-Authors: Corinna Rebnegger, Diethard Mattanovich, Nadine E. Tatto, Josef W. Moser, Alexandra B. Graf, Brigitte GasserAbstract:Many yeasts differentiate into multicellular phenotypes in adverse environmental conditions. Here, we investigate pseudohyphal growth in Komagataella phaffii and the involvement of the flocculin (FLO) gene family in its regulation. The K. phaffii FLO family consists of 13 members, and the conditions inducing pseudohyphal growth are different from Saccharomyces cerevisiae. So far, this phenotype was only observed when K. phaffii was cultivated at slow growth rates in glucose-limited chemostats, but not upon nitrogen starvation or the presence of fusel alcohols. Transcriptional analysis identified that FLO11, FLO400 and FLO5-1 are involved in the phenotype, all being controlled by the transcriptional regulator Flo8. The three genes exhibit a complex mechanism of expression and repression during transition from yeast to pseudohyphal form. Unlike in S. cerevisiae, deletion of FLO11 does not completely prevent the phenotype. In contrast, deletion of FLO400 or FLO5-1 prevents pseudohyphae formation, and hampers FLO11 expression. FAIRE-Seq data shows that the expression and repression of FLO400 and FLO5-1 are correlated to open or closed chromatin regions upstream of these genes, respectively. Our findings indicate that K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 upon glucose limitation in chemostats at slow growth and chromatin modulation is involved in the regulation of their expression.
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microbe profile Komagataella phaffii a methanol devouring biotech yeast formerly known as pichia pastoris
Microbiology, 2020Co-Authors: Lina Heistinger, Brigitte Gasser, Diethard MattanovichAbstract:Methylotrophic yeasts of the genus Komagataella are abundantly found in tree exudates. Their ability to utilize methanol as carbon and energy source relies on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as well. Komagataella yeasts usually grow as haploid cells and are secondary homothallic as they can switch mating type. Upon mating diploid cells sporulate readily, forming asci with four haploid spores. Their ability to secrete high amounts of heterologous proteins made them interesting for biotechnology, which expands today also to other products of primary and secondary metabolism.
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Komagataella phaffii YPS1-5 encodes the alpha-factor degrading protease Bar1.
FEMS yeast research, 2020Co-Authors: Lina Heistinger, Brigitte Gasser, Diethard MattanovichAbstract:Yeast mating pheromones are small secreted peptides required for efficient mating between cells of opposite mating type. Pheromone gradients allow the cells to detect potential mating partners. Secreted pheromone degrading proteases steepen local gradients and allow fast recovery from the pheromone signal. The methylotrophic yeast Komagataella phaffii is a preferentially haploid species. Only under nitrogen starvation, mating genes are activated and the cells are able to undergo a full sexual cycle of mating and sporulation. It has been shown that, similar to other yeasts, K. phaffii requires the mating pheromone and pheromone surface receptor genes for efficient mating. The analysis of so far uncharacterized mating-type-specific genes allowed us to identify the K. phaffii α-factor protease gene YPS1-5. It encodes an aspartic protease of the yapsin family and is upregulated only in a-type cells under mating conditions. The phenotype of K. phaffiia-type strains with a deletion in the protease gene was found to be highly similar to the phenotype of Saccharomyces cerevisiae α-factor protease BAR1 deletion strains. They are highly sensitive to α-factor pheromone in pheromone sensitivity assays and were found to mate with reduced efficiency. Based on our results, we propose to rename the gene into K. phaffii BAR1.
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A yeast for all seasons - Is Pichia pastoris a suitable chassis organism for future bioproduction?
FEMS microbiology letters, 2018Co-Authors: Brigitte Gasser, Diethard MattanovichAbstract:The methylotrophic yeast Pichia pastoris (Komagataella spp.) is well established as a host for recombinant protein production. Since a few years it has also been subject to metabolic engineering to produce a diversity of biochemicals. Based on these developments we discuss here why P. pastoris is a suitable future chassis organism for synthetic biology, and we provide a roadmap for a community effort towards this aim.
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Identification and characterization of the Komagataella phaffii mating pheromone genes.
FEMS yeast research, 2018Co-Authors: Lina Heistinger, Brigitte Gasser, Nadine E. Tatto, Minoska Valli, Josef W. Moser, Diethard MattanovichAbstract:The methylotrophic yeast Komagataella phaffii (Pichia pastoris) is a haploid yeast that is able to form diploid cells by mating once nitrogen becomes limiting. Activation of the mating response requires the secretion of a- and α-factor pheromones, which bind to G-protein coupled receptors on cells of opposite mating type. In K. phaffii, the genes coding for the α-factor (MFα), the pheromone surface receptors and the conserved a-factor biogenesis pathway have been annotated previously. Initial homology-based search failed to identify potential a-factor genes (MFA). By using transcriptome data of heterothallic strains under mating conditions, we found two K. phaffiia-factor genes. Deletion of both MFA genes prevented mating of a-type cells. MFA single mutants were still able to mate and activate the mating response pathway in α-type cells. A reporter assay was used to confirm the biological activity of synthetic a- and α-factor peptides. The identification of the a-factor genes enabled the first characterization of the role and regulation of the mating pheromone genes and the response of K. phaffii to synthetic pheromones and will help to gain a better understanding of the mating behavior of K. phaffii.
Maria A.m. Reis - One of the best experts on this subject based on the ideXlab platform.
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Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source.
Carbohydrate polymers, 2017Co-Authors: Diana Araújo, Christian Grandfils, Filomena Freitas, Chantal Sevrin, Maria A.m. ReisAbstract:Abstract Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46–0.54 g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64 g/L, with a yield on xylose of 0.52 g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.
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chitin glucan complex production by Komagataella pastoris downstream optimization and product characterization
Carbohydrate Polymers, 2015Co-Authors: Inês Farinha, Paulo Duarte, Ana Pimentel, Evgeniya Plotnikova, Ferreira Chagas Bárbara, Luís Mafra, Christian Grandfils, Filomena Freitas, Elvira Fortunato, Maria A.m. ReisAbstract:Abstract Purified chitin–glucan complex (CGC pure ) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGC pure had an average molecular weight of 4.9 × 10 5 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGC pure has α-chitin in its structure. CGC pure presented an endothermic decomposition peak at 315 °C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.
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Chitin–glucan complex production by Komagataella pastoris: Downstream optimization and product characterization
Carbohydrate polymers, 2015Co-Authors: Inês Farinha, Paulo Duarte, Ana Pimentel, Evgeniya Plotnikova, Ferreira Chagas Bárbara, Luís Mafra, Christian Grandfils, Filomena Freitas, Elvira Fortunato, Maria A.m. ReisAbstract:Abstract Purified chitin–glucan complex (CGC pure ) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGC pure had an average molecular weight of 4.9 × 10 5 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGC pure has α-chitin in its structure. CGC pure presented an endothermic decomposition peak at 315 °C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.
Minoska Valli - One of the best experts on this subject based on the ideXlab platform.
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A subcellular proteome atlas of the yeast Komagataella phaffii.
FEMS yeast research, 2020Co-Authors: Minoska Valli, Nadine E. Tatto, Karlheinz Grillitsch, Clemens Grünwald-gruber, Bernhard Andreas Hrobath, Lisa Klug, Vasyl Ivashov, Sandra Hauzmayer, Martina Koller, Nora TirAbstract:The compartmentalization of metabolic and regulatory pathways is a common pattern of living organisms. Eukaryotic cells are subdivided into several organelles enclosed by lipid membranes. Organelle proteomes define their functions. Yeasts, as simple eukaryotic single cell organisms, are valuable models for higher eukaryotes and frequently used for biotechnological applications. While the subcellular distribution of proteins is well studied in Saccharomyces cerevisiae, this is not the case for other yeasts like Komagataella phaffii (syn. Pichia pastoris). Different to most well-studied yeasts, K. phaffii can grow on methanol, which provides specific features for production of heterologous proteins and as a model for peroxisome biology. We isolated microsomes, very early Golgi, early Golgi, plasma membrane, vacuole, cytosol, peroxisomes and mitochondria of K. phaffii from glucose- and methanol-grown cultures, quantified their proteomes by liquid chromatography-electrospray ionization-mass spectrometry of either unlabeled or tandem mass tag-labeled samples. Classification of the proteins by their relative enrichment, allowed the separation of enriched proteins from potential contaminants in all cellular compartments except the peroxisomes. We discuss differences to S. cerevisiae, outline organelle specific findings and the major metabolic pathways and provide an interactive map of the subcellular localization of proteins in K. phaffii.
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a single gal4 like transcription factor activates the crabtree effect in Komagataella phaffii
Nature Communications, 2018Co-Authors: Özge Ata, Corinna Rebnegger, Nadine E. Tatto, Minoska Valli, Teresa Mairinger, Stephan Hann, Matthias G. Steiger, Pınar ÇalıkAbstract:The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates. It provides Crabtree positive yeasts an evolutionary advantage of consuming glucose faster and producing ethanol to outcompete other microorganisms in sugar rich environments. While a number of genetic events are associated with the emergence of the Crabtree effect, its evolution remains unresolved. Here we show that overexpression of a single Gal4-like transcription factor is sufficient to convert Crabtree-negative Komagataella phaffii (Pichia pastoris) into a Crabtree positive yeast. Upregulation of the glycolytic genes and a significant increase in glucose uptake rate due to the overexpression of the Gal4-like transcription factor leads to an overflow metabolism, triggering both short-term and long-term Crabtree phenotypes. This indicates that a single genetic perturbation leading to overexpression of one gene may have been sufficient as the first molecular event towards respiro-fermentative metabolism in the course of yeast evolution.
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Identification and characterization of the Komagataella phaffii mating pheromone genes.
FEMS yeast research, 2018Co-Authors: Lina Heistinger, Brigitte Gasser, Nadine E. Tatto, Minoska Valli, Josef W. Moser, Diethard MattanovichAbstract:The methylotrophic yeast Komagataella phaffii (Pichia pastoris) is a haploid yeast that is able to form diploid cells by mating once nitrogen becomes limiting. Activation of the mating response requires the secretion of a- and α-factor pheromones, which bind to G-protein coupled receptors on cells of opposite mating type. In K. phaffii, the genes coding for the α-factor (MFα), the pheromone surface receptors and the conserved a-factor biogenesis pathway have been annotated previously. Initial homology-based search failed to identify potential a-factor genes (MFA). By using transcriptome data of heterothallic strains under mating conditions, we found two K. phaffiia-factor genes. Deletion of both MFA genes prevented mating of a-type cells. MFA single mutants were still able to mate and activate the mating response pathway in α-type cells. A reporter assay was used to confirm the biological activity of synthetic a- and α-factor peptides. The identification of the a-factor genes enabled the first characterization of the role and regulation of the mating pheromone genes and the response of K. phaffii to synthetic pheromones and will help to gain a better understanding of the mating behavior of K. phaffii.
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A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii
Nature Publishing Group, 2018Co-Authors: Özge Ata, Corinna Rebnegger, Nadine E. Tatto, Minoska Valli, Teresa Mairinger, Stephan Hann, Matthias G. Steiger, Pınar Çalık, Diethard MattanovichAbstract:Aerobic ethanol production, a phenomenon referred as Crabtree effect, allows yeast to outcompete other microorganisms in sugar rich environments. Here, the authors show that overexpression of a Gal4-like transcription factor can transform Komagataella phaffii from Crabtree effect negative to positive
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systems level organization of yeast methylotrophic lifestyle
BMC Biology, 2015Co-Authors: Hannes Rusmayer, Minoska Valli, Markus Buchetics, Clemens Gruber, Karlheinz Grillitsch, Gerda Modarres, Raffaele Guerrasio, Kristaps Klavins, Stefan NeubauerAbstract:Background Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes such as alcohol oxidase and dihydroxyacetone synthase and their localization to the peroxisomes. C1 assimilation is supposed to involve the pentose phosphate pathway, but details of these reactions are not known to date.
