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Raquel Vilela - One of the best experts on this subject based on the ideXlab platform.
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Paracoccidioidomycosis ceti (Lacaziosis/Lobomycosis) in Dolphins
Emerging and Epizootic Fungal Infections in Animals, 2018Co-Authors: Raquel Vilela, Leonel MendozaAbstract:Infections caused by the fungal pathogen Lacazia Loboi were first reported in 1931 by Jorge de Oliveira Lobo in a human with granulomatous skin lesions in Pernambuco, Brazil. Early histopathological and serological analyses found morphological similarities and cross-reactive antigens with Paracoccidioides brasiliensis. In 1971, veterinarians working with dolphins in Florida, USA, reported granulomatous skin lesions in a dolphin, similar to that in human lacaziosis. Based on histopathological findings, L. Loboi was initially believed to be also the etiologic agent of cutaneous disease in dolphins. Ever since, cutaneous granulomas have been reported in different dolphin species around the coast of Asia, Europe, and North and South America. Recently, using molecular biology approaches, some investigators stated that the DNA sequences extracted from cases of cutaneous granulomas in dolphins were closely related to those of P. brasiliensis. This chapter deals with the history, taxonomy, and other features of L. Loboi in humans and the unculturable P. brasiliensis var. ceti type affecting the skin of dolphins.
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cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis
Emerging Infectious Diseases, 2016Co-Authors: Raquel Vilela, Judy St. Leger, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Adam M. Schaefer, Leslie M Dalton, Peter J Mccarthy, Leonel MendozaAbstract:Cutaneous granulomas in dolphins were believed to be caused by Lacazia Loboi, which also causes a similar disease in humans. This hypothesis was recently challenged by reports that fungal DNA sequences from dolphins grouped this pathogen with Paracoccidioides brasiliensis. We conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (Tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. Kex gene sequences of P. brasiliensis from dolphins showed 100% homology with sequences from cultivated P. brasiliensis, 73% with those of L. Loboi, and 93% with those of P. lutzii. Parsimony analysis placed DNA sequences from dolphins within a cluster with human P. brasiliensis strains. This cluster was the sister taxon to P. lutzii and L. Loboi. Our molecular data support previous findings and suggest that a novel uncultivated strain of P. brasiliensis restricted to cutaneous lesions in dolphins is probably the cause of lacaziosis/lobomycosis, herein referred to as paracoccidioidomycosis ceti.
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Clin. Vaccine Immunol. doi:10.1128/CVI.00201-07 Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. 1 2 3 4 5 6 7 8
2013Co-Authors: Leonel Mendoza, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, John S. Reif, Patricia A. Fair, Andréa F. F. Belone, Gregory D, Wendy N. Durden, Luiz RAbstract:20 Western blot analyses of Lacazia Loboi antigens using sera from humans and dolphins with lacaziosis and sera from experimentally infected mic
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Molecular phylogeny of animal pathogen Lacazia Loboi inferred from rDNA and DNA coding sequences
Fungal Biology, 2009Co-Authors: Raquel Vilela, Patrícia Sammarco Rosa, Andréa De Faria Fernandes Belone, Suzana Madeira Diório, John W. Taylor, Leonel MendozaAbstract:Lacazia Loboi is a geographically restricted, uncultivated fungal pathogen of humans and dolphins. Previous investigations using 18S small unit rDNA, chitin synthase 2 and gp43 DNA sequences positioned L. Loboi as a close relative of Paracoccidioides brasiliensis. However, given the few individuals of L. Loboi studied and the high degree of genetic variation observed in P. brasiliensis, the existence of L. Loboi as an independent species has been questioned. To investigate the phylogenetic position of this species, we conducted a phylogenetic analysis using 20 L. Loboi collections (L. Loboi was obtained from proven cases of lacaziosis and 14 collections were maintained in mice, the others were analyzed from DNA taken directly from infected human tissue.). L. Loboi DNA sequence was compared to that from 17 P. brasiliensis strains that represented the known variation in this species, and outgroup taxa in the Onygenales (Ajellomyces and Coccidioides species). Our analyses used DNA sequence from ITS rRNA, and partial coding sequences of chitin synthase 4, ADP-ribosylation factor, and gp43. Nucleotide variation among strains of L. Loboi was minor but numerous nucleotide mismatches and multiple gaps were found for these gene regions among members in the Ajellomycetaceae, including P. brasiliensis. Phylogenies inferred using neighbor-joining, maximum parsimony and Bayesian analyses showed no significant conflict and depicted L. Loboi as a well-supported, monophyletic group that was sister to the Paracoccidioides clade. These results argue for maintaining L. Loboi as a taxon independent from Paracoccidioides within the Ajellomycetaceae.
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Accidental Jorge Lobo's disease in a worker dealing with Lacazia Loboi infected mice: a case report
Journal of Medical Case Reports, 2009Co-Authors: Patrícia Sammarco Rosa, Raquel Vilela, A.f.f. Belone, Cleverson Teixeira Soares, Somei Ura, Milton Cury Filho, Leonel MendozaAbstract:Introduction Jorge Lobo's disease (Lacaziosis) is a subcutaneous infection of humans living in the Amazon region of Latin America, and in dolphins inhabiting the east coastal areas of the United States. The disease mainly affects people from rural areas living or working in close contact with vegetation and aquatic environments. Most patients refer having developed lesions after accidental trauma with plant thorns or insect bites. Inter-human transmission has never been confirmed suggesting that Lacazia Loboi is acquired from environmental propagules. Case presentation We report the case of a 41-year-old woman from São Paulo, Brazil, a non-endemic area of Jorge Lobo's disease, with L. Loboi skin infection most likely accidentally acquired while manipulating experimentally infected mice in the laboratory. Conclusion Because many patients with Jorge Lobo's disease do not recall accidental skin trauma before their infections, the possibility of accidentally acquired Jorge Lobo's disease through unnoticed broken skin should be considered during the clinical investigation of nodular skin diseases in people who have contact with the fungus or who live in endemic areas. This is the second report of animal to human transmission of this disease.
Leonel Mendoza - One of the best experts on this subject based on the ideXlab platform.
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Paracoccidioidomycosis ceti (Lacaziosis/Lobomycosis) in Dolphins
Emerging and Epizootic Fungal Infections in Animals, 2018Co-Authors: Raquel Vilela, Leonel MendozaAbstract:Infections caused by the fungal pathogen Lacazia Loboi were first reported in 1931 by Jorge de Oliveira Lobo in a human with granulomatous skin lesions in Pernambuco, Brazil. Early histopathological and serological analyses found morphological similarities and cross-reactive antigens with Paracoccidioides brasiliensis. In 1971, veterinarians working with dolphins in Florida, USA, reported granulomatous skin lesions in a dolphin, similar to that in human lacaziosis. Based on histopathological findings, L. Loboi was initially believed to be also the etiologic agent of cutaneous disease in dolphins. Ever since, cutaneous granulomas have been reported in different dolphin species around the coast of Asia, Europe, and North and South America. Recently, using molecular biology approaches, some investigators stated that the DNA sequences extracted from cases of cutaneous granulomas in dolphins were closely related to those of P. brasiliensis. This chapter deals with the history, taxonomy, and other features of L. Loboi in humans and the unculturable P. brasiliensis var. ceti type affecting the skin of dolphins.
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cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis
Emerging Infectious Diseases, 2016Co-Authors: Raquel Vilela, Judy St. Leger, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Adam M. Schaefer, Leslie M Dalton, Peter J Mccarthy, Leonel MendozaAbstract:Cutaneous granulomas in dolphins were believed to be caused by Lacazia Loboi, which also causes a similar disease in humans. This hypothesis was recently challenged by reports that fungal DNA sequences from dolphins grouped this pathogen with Paracoccidioides brasiliensis. We conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (Tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. Kex gene sequences of P. brasiliensis from dolphins showed 100% homology with sequences from cultivated P. brasiliensis, 73% with those of L. Loboi, and 93% with those of P. lutzii. Parsimony analysis placed DNA sequences from dolphins within a cluster with human P. brasiliensis strains. This cluster was the sister taxon to P. lutzii and L. Loboi. Our molecular data support previous findings and suggest that a novel uncultivated strain of P. brasiliensis restricted to cutaneous lesions in dolphins is probably the cause of lacaziosis/lobomycosis, herein referred to as paracoccidioidomycosis ceti.
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Clin. Vaccine Immunol. doi:10.1128/CVI.00201-07 Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. 1 2 3 4 5 6 7 8
2013Co-Authors: Leonel Mendoza, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, John S. Reif, Patricia A. Fair, Andréa F. F. Belone, Gregory D, Wendy N. Durden, Luiz RAbstract:20 Western blot analyses of Lacazia Loboi antigens using sera from humans and dolphins with lacaziosis and sera from experimentally infected mic
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Molecular phylogeny of animal pathogen Lacazia Loboi inferred from rDNA and DNA coding sequences
Fungal Biology, 2009Co-Authors: Raquel Vilela, Patrícia Sammarco Rosa, Andréa De Faria Fernandes Belone, Suzana Madeira Diório, John W. Taylor, Leonel MendozaAbstract:Lacazia Loboi is a geographically restricted, uncultivated fungal pathogen of humans and dolphins. Previous investigations using 18S small unit rDNA, chitin synthase 2 and gp43 DNA sequences positioned L. Loboi as a close relative of Paracoccidioides brasiliensis. However, given the few individuals of L. Loboi studied and the high degree of genetic variation observed in P. brasiliensis, the existence of L. Loboi as an independent species has been questioned. To investigate the phylogenetic position of this species, we conducted a phylogenetic analysis using 20 L. Loboi collections (L. Loboi was obtained from proven cases of lacaziosis and 14 collections were maintained in mice, the others were analyzed from DNA taken directly from infected human tissue.). L. Loboi DNA sequence was compared to that from 17 P. brasiliensis strains that represented the known variation in this species, and outgroup taxa in the Onygenales (Ajellomyces and Coccidioides species). Our analyses used DNA sequence from ITS rRNA, and partial coding sequences of chitin synthase 4, ADP-ribosylation factor, and gp43. Nucleotide variation among strains of L. Loboi was minor but numerous nucleotide mismatches and multiple gaps were found for these gene regions among members in the Ajellomycetaceae, including P. brasiliensis. Phylogenies inferred using neighbor-joining, maximum parsimony and Bayesian analyses showed no significant conflict and depicted L. Loboi as a well-supported, monophyletic group that was sister to the Paracoccidioides clade. These results argue for maintaining L. Loboi as a taxon independent from Paracoccidioides within the Ajellomycetaceae.
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Accidental Jorge Lobo's disease in a worker dealing with Lacazia Loboi infected mice: a case report
Journal of Medical Case Reports, 2009Co-Authors: Patrícia Sammarco Rosa, Raquel Vilela, A.f.f. Belone, Cleverson Teixeira Soares, Somei Ura, Milton Cury Filho, Leonel MendozaAbstract:Introduction Jorge Lobo's disease (Lacaziosis) is a subcutaneous infection of humans living in the Amazon region of Latin America, and in dolphins inhabiting the east coastal areas of the United States. The disease mainly affects people from rural areas living or working in close contact with vegetation and aquatic environments. Most patients refer having developed lesions after accidental trauma with plant thorns or insect bites. Inter-human transmission has never been confirmed suggesting that Lacazia Loboi is acquired from environmental propagules. Case presentation We report the case of a 41-year-old woman from São Paulo, Brazil, a non-endemic area of Jorge Lobo's disease, with L. Loboi skin infection most likely accidentally acquired while manipulating experimentally infected mice in the laboratory. Conclusion Because many patients with Jorge Lobo's disease do not recall accidental skin trauma before their infections, the possibility of accidentally acquired Jorge Lobo's disease through unnoticed broken skin should be considered during the clinical investigation of nodular skin diseases in people who have contact with the fungus or who live in endemic areas. This is the second report of animal to human transmission of this disease.
John S. Reif - One of the best experts on this subject based on the ideXlab platform.
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cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis
Emerging Infectious Diseases, 2016Co-Authors: Raquel Vilela, Judy St. Leger, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Adam M. Schaefer, Leslie M Dalton, Peter J Mccarthy, Leonel MendozaAbstract:Cutaneous granulomas in dolphins were believed to be caused by Lacazia Loboi, which also causes a similar disease in humans. This hypothesis was recently challenged by reports that fungal DNA sequences from dolphins grouped this pathogen with Paracoccidioides brasiliensis. We conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (Tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. Kex gene sequences of P. brasiliensis from dolphins showed 100% homology with sequences from cultivated P. brasiliensis, 73% with those of L. Loboi, and 93% with those of P. lutzii. Parsimony analysis placed DNA sequences from dolphins within a cluster with human P. brasiliensis strains. This cluster was the sister taxon to P. lutzii and L. Loboi. Our molecular data support previous findings and suggest that a novel uncultivated strain of P. brasiliensis restricted to cutaneous lesions in dolphins is probably the cause of lacaziosis/lobomycosis, herein referred to as paracoccidioidomycosis ceti.
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Toward the identification, characterization and experimental culture of Lacazia Loboi from Atlantic bottlenose dolphin ( Tursiops truncatus )
Medical mycology, 2016Co-Authors: Adam M. Schaefer, Gregory D. Bossart, John S. Reif, Esther A. Guzmán, Patrick Ottuso, Joseph Snyder, Neil S Medalie, Ralph Rosato, Sushan Han, Patricia A. FairAbstract:Lobomycosis (lacaziosis) is a chronic, granulomatous, fungal infection of the skin and subcutaneous tissues of humans and dolphins. To date, the causative agent, the yeast-like organism Lacazia Loboi, has not been grown in the laboratory, and there have been no recent reports describing attempts to culture the organism. As a result, studies on the efficacy of therapeutics and potential environmental reservoirs have not been conducted. Therefore, the objective of the current study was to utilize both classical and novel microbiological methods in order to stimulate growth of Lacazia cells collected from dolphin lesions. This included the experimental inoculation of novel media, cell culture, and the use of artificial skin matrices. Although unsuccessful, the methods and results of this study provide important insight into new approaches that could be utilized in future investigations of this elusive organism.
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Clin. Vaccine Immunol. doi:10.1128/CVI.00201-07 Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. 1 2 3 4 5 6 7 8
2013Co-Authors: Leonel Mendoza, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, John S. Reif, Patricia A. Fair, Andréa F. F. Belone, Gregory D, Wendy N. Durden, Luiz RAbstract:20 Western blot analyses of Lacazia Loboi antigens using sera from humans and dolphins with lacaziosis and sera from experimentally infected mic
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Lobomycosis: risk of zoonotic transmission from dolphins to humans.
Vector borne and zoonotic diseases (Larchmont N.Y.), 2013Co-Authors: John S. Reif, Adam M. Schaefer, Gregory D. BossartAbstract:Abstract Lobomycosis, a fungal disease of the skin and subcutaneous tissues caused by Lacazia Loboi, is sometimes referred to as a zoonotic disease because it affects only specific delphinidae and humans; however, the evidence that it can be transferred directly to humans from dolphins is weak. Dolphins have also been postulated to be responsible for an apparent geographic expansion of the disease in humans. Morphological and molecular differences between the human and dolphin organisms, differences in geographic distribution of the diseases between dolphins and humans, the existence of only a single documented case of presumed zoonotic transmission, and anecdotal evidence of lack of transmission to humans following accidental inoculation of tissue from infected dolphins do not support the hypothesis that dolphins infected with L. Loboi represent a zoonotic hazard for humans. In addition, the lack of human cases in communities adjacent to coastal estuaries with a high prevalence of lobomycosis in dolphins...
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Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for Western Blot analyses of Lacazia Loboi antigens.
Clinical and vaccine immunology : CVI, 2007Co-Authors: Leonel Mendoza, Wendy Noke Durden, Andréa De Faria Fernandes Belone, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Luiz R. TravassosAbstract:Antibodies in the sera of patients with lacaziosis recognized an approximately 193-kDa antigen and other Lacazia Loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. Loboi extracts. This study is the first to examine the humoral response to L. Loboi antigens by using multiple host sera.
Gregory D. Bossart - One of the best experts on this subject based on the ideXlab platform.
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cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis
Emerging Infectious Diseases, 2016Co-Authors: Raquel Vilela, Judy St. Leger, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Adam M. Schaefer, Leslie M Dalton, Peter J Mccarthy, Leonel MendozaAbstract:Cutaneous granulomas in dolphins were believed to be caused by Lacazia Loboi, which also causes a similar disease in humans. This hypothesis was recently challenged by reports that fungal DNA sequences from dolphins grouped this pathogen with Paracoccidioides brasiliensis. We conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (Tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. Kex gene sequences of P. brasiliensis from dolphins showed 100% homology with sequences from cultivated P. brasiliensis, 73% with those of L. Loboi, and 93% with those of P. lutzii. Parsimony analysis placed DNA sequences from dolphins within a cluster with human P. brasiliensis strains. This cluster was the sister taxon to P. lutzii and L. Loboi. Our molecular data support previous findings and suggest that a novel uncultivated strain of P. brasiliensis restricted to cutaneous lesions in dolphins is probably the cause of lacaziosis/lobomycosis, herein referred to as paracoccidioidomycosis ceti.
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Toward the identification, characterization and experimental culture of Lacazia Loboi from Atlantic bottlenose dolphin ( Tursiops truncatus )
Medical mycology, 2016Co-Authors: Adam M. Schaefer, Gregory D. Bossart, John S. Reif, Esther A. Guzmán, Patrick Ottuso, Joseph Snyder, Neil S Medalie, Ralph Rosato, Sushan Han, Patricia A. FairAbstract:Lobomycosis (lacaziosis) is a chronic, granulomatous, fungal infection of the skin and subcutaneous tissues of humans and dolphins. To date, the causative agent, the yeast-like organism Lacazia Loboi, has not been grown in the laboratory, and there have been no recent reports describing attempts to culture the organism. As a result, studies on the efficacy of therapeutics and potential environmental reservoirs have not been conducted. Therefore, the objective of the current study was to utilize both classical and novel microbiological methods in order to stimulate growth of Lacazia cells collected from dolphin lesions. This included the experimental inoculation of novel media, cell culture, and the use of artificial skin matrices. Although unsuccessful, the methods and results of this study provide important insight into new approaches that could be utilized in future investigations of this elusive organism.
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Lobomycosis: risk of zoonotic transmission from dolphins to humans.
Vector borne and zoonotic diseases (Larchmont N.Y.), 2013Co-Authors: John S. Reif, Adam M. Schaefer, Gregory D. BossartAbstract:Abstract Lobomycosis, a fungal disease of the skin and subcutaneous tissues caused by Lacazia Loboi, is sometimes referred to as a zoonotic disease because it affects only specific delphinidae and humans; however, the evidence that it can be transferred directly to humans from dolphins is weak. Dolphins have also been postulated to be responsible for an apparent geographic expansion of the disease in humans. Morphological and molecular differences between the human and dolphin organisms, differences in geographic distribution of the diseases between dolphins and humans, the existence of only a single documented case of presumed zoonotic transmission, and anecdotal evidence of lack of transmission to humans following accidental inoculation of tissue from infected dolphins do not support the hypothesis that dolphins infected with L. Loboi represent a zoonotic hazard for humans. In addition, the lack of human cases in communities adjacent to coastal estuaries with a high prevalence of lobomycosis in dolphins...
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Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for Western Blot analyses of Lacazia Loboi antigens.
Clinical and vaccine immunology : CVI, 2007Co-Authors: Leonel Mendoza, Wendy Noke Durden, Andréa De Faria Fernandes Belone, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Luiz R. TravassosAbstract:Antibodies in the sera of patients with lacaziosis recognized an approximately 193-kDa antigen and other Lacazia Loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. Loboi extracts. This study is the first to examine the humoral response to L. Loboi antigens by using multiple host sera.
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Lobomycosis in Atlantic bottlenose dolphins from the Indian River Lagoon, Florida
Journal of the American Veterinary Medical Association, 2006Co-Authors: John S. Reif, Patricia A. Fair, Marilyn Mazzoil, Stephen D. Mcculloch, René A. Varela, Juli D. Goldstein, Gregory D. BossartAbstract:Objective—To determine the prevalence of lobomycosis, a mycotic infection of dolphins and humans caused by a yeastlike organism (Lacazia Loboi), among dolphins in the Indian River Lagoon in Florida. Design—Cross-sectional study. Animals—146 Atlantic bottlenose dolphins. Procedure—Comprehensive health assessments of bottlenose dolphins in the Indian River Lagoon of Florida (n = 75) and in estuarine waters near Charleston, SC (71), were conducted during 2003 and 2004. Bottlenose dolphins were captured, examined, and released. Skin lesions were photographed and then biopsied. Tissue sections were stained with H&E and Gomori methenamine silver stains for identification of L Loboi. Results—9 of 30 (30%) dolphins captured in the southern portion of the Indian River Lagoon had lobomycosis, whereas none of the 45 dolphins captured in the northern portion of the lagoon or of the 71 dolphins captured near Charleston, SC, did. Affected dolphins had low serum alkaline phosphatase activities and high acute-phase prote...
Patricia A. Fair - One of the best experts on this subject based on the ideXlab platform.
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cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis
Emerging Infectious Diseases, 2016Co-Authors: Raquel Vilela, Judy St. Leger, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Adam M. Schaefer, Leslie M Dalton, Peter J Mccarthy, Leonel MendozaAbstract:Cutaneous granulomas in dolphins were believed to be caused by Lacazia Loboi, which also causes a similar disease in humans. This hypothesis was recently challenged by reports that fungal DNA sequences from dolphins grouped this pathogen with Paracoccidioides brasiliensis. We conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (Tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. Kex gene sequences of P. brasiliensis from dolphins showed 100% homology with sequences from cultivated P. brasiliensis, 73% with those of L. Loboi, and 93% with those of P. lutzii. Parsimony analysis placed DNA sequences from dolphins within a cluster with human P. brasiliensis strains. This cluster was the sister taxon to P. lutzii and L. Loboi. Our molecular data support previous findings and suggest that a novel uncultivated strain of P. brasiliensis restricted to cutaneous lesions in dolphins is probably the cause of lacaziosis/lobomycosis, herein referred to as paracoccidioidomycosis ceti.
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Toward the identification, characterization and experimental culture of Lacazia Loboi from Atlantic bottlenose dolphin ( Tursiops truncatus )
Medical mycology, 2016Co-Authors: Adam M. Schaefer, Gregory D. Bossart, John S. Reif, Esther A. Guzmán, Patrick Ottuso, Joseph Snyder, Neil S Medalie, Ralph Rosato, Sushan Han, Patricia A. FairAbstract:Lobomycosis (lacaziosis) is a chronic, granulomatous, fungal infection of the skin and subcutaneous tissues of humans and dolphins. To date, the causative agent, the yeast-like organism Lacazia Loboi, has not been grown in the laboratory, and there have been no recent reports describing attempts to culture the organism. As a result, studies on the efficacy of therapeutics and potential environmental reservoirs have not been conducted. Therefore, the objective of the current study was to utilize both classical and novel microbiological methods in order to stimulate growth of Lacazia cells collected from dolphin lesions. This included the experimental inoculation of novel media, cell culture, and the use of artificial skin matrices. Although unsuccessful, the methods and results of this study provide important insight into new approaches that could be utilized in future investigations of this elusive organism.
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Clin. Vaccine Immunol. doi:10.1128/CVI.00201-07 Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. 1 2 3 4 5 6 7 8
2013Co-Authors: Leonel Mendoza, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, John S. Reif, Patricia A. Fair, Andréa F. F. Belone, Gregory D, Wendy N. Durden, Luiz RAbstract:20 Western blot analyses of Lacazia Loboi antigens using sera from humans and dolphins with lacaziosis and sera from experimentally infected mic
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Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for Western Blot analyses of Lacazia Loboi antigens.
Clinical and vaccine immunology : CVI, 2007Co-Authors: Leonel Mendoza, Wendy Noke Durden, Andréa De Faria Fernandes Belone, Raquel Vilela, Judy St. Leger, Manuela Rehtanz, Gregory D. Bossart, John S. Reif, Patricia A. Fair, Luiz R. TravassosAbstract:Antibodies in the sera of patients with lacaziosis recognized an approximately 193-kDa antigen and other Lacazia Loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. Loboi extracts. This study is the first to examine the humoral response to L. Loboi antigens by using multiple host sera.
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Lobomycosis in Atlantic bottlenose dolphins from the Indian River Lagoon, Florida
Journal of the American Veterinary Medical Association, 2006Co-Authors: John S. Reif, Patricia A. Fair, Marilyn Mazzoil, Stephen D. Mcculloch, René A. Varela, Juli D. Goldstein, Gregory D. BossartAbstract:Objective—To determine the prevalence of lobomycosis, a mycotic infection of dolphins and humans caused by a yeastlike organism (Lacazia Loboi), among dolphins in the Indian River Lagoon in Florida. Design—Cross-sectional study. Animals—146 Atlantic bottlenose dolphins. Procedure—Comprehensive health assessments of bottlenose dolphins in the Indian River Lagoon of Florida (n = 75) and in estuarine waters near Charleston, SC (71), were conducted during 2003 and 2004. Bottlenose dolphins were captured, examined, and released. Skin lesions were photographed and then biopsied. Tissue sections were stained with H&E and Gomori methenamine silver stains for identification of L Loboi. Results—9 of 30 (30%) dolphins captured in the southern portion of the Indian River Lagoon had lobomycosis, whereas none of the 45 dolphins captured in the northern portion of the lagoon or of the 71 dolphins captured near Charleston, SC, did. Affected dolphins had low serum alkaline phosphatase activities and high acute-phase prote...
