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Svetlana P. Ermakova - One of the best experts on this subject based on the ideXlab platform.
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Aminated Laminaran from brown alga Saccharina cichorioides: Synthesis, structure, anticancer, and radiosensitizing potential in vitro
Carbohydrate polymers, 2020Co-Authors: Olesya S. Malyarenko, Roza V. Usoltseva, Artem S. Silchenko, Svetlana P. ErmakovaAbstract:Abstract Laminarans are currently the focus of attention in regard to the selection of prospective agents for the prevention and treatment of cancer. Laminaran from Saccharina cichorioides was aminated to heighten anticancer and radiosensitizing activities and elucidate its molecular mode of action. Aminated Laminaran, ScLNH2, was identified as 1,3-β- d -glucan with −CH2−CH(OH)−CH2-NH2 group at the C6 of branches. ScLNH2 selectively inhibited the viability and colony formation in the MDA-MB-231 cell line of triple negative breast cancer cells. ScLNH2 possessed synergism with radiation, resulting in a decreased number of colonies of MDA-MB-231 cells. The mechanism underling the radiosensitizing effect of ScLNH2 was associated with apoptosis induction via regulation of caspases 9 and 3 and PARP enzyme, preventing the repair of DNA damage in irradiated cells. These findings confirmed that combination therapy by aminated Laminaran and radiation might play a role in the optimization of therapy for an aggressive form of human breast cancer.
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Composition of polysaccharides and radiosensitizing activity of native and sulfated Laminarans from the Tаuуа basicrassa Kloczc. et Krupn.
Carbohydrate polymers, 2020Co-Authors: Tatiana N Zvyagintseva, Tatiana N Krupnova, Roza V. Usoltseva, Natalia M. Shevchenko, Vladimir V. Isakov, Stanislav D. Anastyuk, Nikolai V. Zvyagintsev, Pavel A. Zadorozhny, Svetlana P. ErmakovaAbstract:Abstract Polysaccharide fractions of alginate, Laminarans and fucoidans were obtained from the brown alga Tauya basicrassa. Yields of alginate and Laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant. Alginate and Laminarans had typical structures for those substances. Fucoidans were low- and medium-sulfated heterogeneous polysaccharides. The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β- d -galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues. Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues. Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6. It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.
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Laminarans and 1,3-β-D-glucanases.
International journal of biological macromolecules, 2020Co-Authors: Roza V. Usoltseva, Olesya S. Malyarenko, Mikhail I. Kusaykin, Aleksei A. Belik, Tatiana N. Zvyagintsevа, Svetlana P. ErmakovaAbstract:The Laminarans are biologically active water-soluble polysaccharide (1,3;1,6-β-D-glucans) of brown algae. These polysaccharides are an attractive object for research due to its relatively simple structure, low toxicity, and various biological effects. 1,3-β-D-glucanases are an effective tool for studying the structure of Laminarans, and can also be used to obtain new biologically active derivatives. This review is to outline what is currently known about Laminarans and enzymes that catalyze of their transformation. We focused on information about sources, structure and properties of Laminarans and 1,3-β-D-glucanases, methods of obtaining and structural elucidation of Laminarans, and biological activity of Laminarans and products of their enzymatic transformation. It has an increased focus on the immunomodulating and anticancer activity of Laminarans and their derivatives.
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Laminaran from brown alga dictyota dichotoma and its sulfated derivative as radioprotectors and radiosensitizers in melanoma therapy
Carbohydrate Polymers, 2019Co-Authors: Olesya S. Malyarenko, Roza V. Usoltseva, T N Zvyagintseva, Svetlana P. ErmakovaAbstract:Abstract The Laminarans are neutral water-soluble β-D-glucans of brown algae possessing potent immunomodulating, radioprotective, and anticancer activities. The aim of the present study was to investigate in vitro anticancer, radioprotective, and radiosensitizing activities of Laminaran from brown alga Dictyota dichotoma and its sulfated derivative. The native and sulfated Laminarans by themselves at non-toxic doses possessed significant anticancer activity against melanoma cells. Both polysaccharides protected normal epidermal cells, while only sulfated Laminaran was able to sensitize melanoma cells to X-rays irradiation resulting in significant inhibition of cell proliferation, colony formation, and migration of cancer cells. The molecular mechanism of this action was related to the inhibition of MMP-2 and MMP-9 proteinases activity as well as down-regulation of kinases’ phosphorylation of ERK1/2 signaling cascade. Taken together, the combination of sulfated derivative of Laminaran from D. dichotoma with X-ray may serve as a potential treatment strategy for human melanoma.
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In vitro anticancer activity of the Laminarans from Far Eastern brown seaweeds and their sulfated derivatives
Journal of Applied Phycology, 2016Co-Authors: Olesya S. Malyarenko, Tatyana N. Zvyagintseva, Roza V. Usoltseva, Natalia M. Shevchenko, Vladimir V. Isakov, Svetlana P. ErmakovaAbstract:Brown seaweeds have drawn worldwide attention due to their involvement in many industrial applications. They produce neutral low molecular weight polysaccharides (Laminarans) with beneficial biological activities. However, the anticancer activity and molecular mechanism of the Laminarans and their sulfated derivatives have not been investigated in detail. Herein, the Laminarans from brown seaweeds Saccharina cichorioides, Saccharina japonica, and Fucus evanescens were isolated. The Laminarans from S. cichorioides and S. japonica were confirmed to contain a main chain of β-(1→3)-D-glucopyranose with single branches at C6. The Laminaran from F. evanescens consisted of not only β-(1→3)-linked D- glucopyranose but also includes single β-(1→6)-linked D-glucose residues. The branches at C6 are presented as glucose or as gentiobiose. The sulfated Laminarans with different degrees of sulfation were obtained by the chlorosulfonic acid-pyridine assay. In modified polysaccharides, the positions of sulfates are directly predetermined by the structure of native Laminarans. The Laminarans and their sulfated derivatives inhibited proliferation, colony formation, and migration of human colorectal adenocarcinoma, melanoma, and breast adenocarcinoma cells in different manners. The sulfated Laminaran from F. evanescens possessed the highest anticancer activity in vitro and effectively prevented migration of breast adenocarcinoma cells by inhibiting of the Matrix Metalloproteinases 2 and 9 activity.
Martin Beaulieu - One of the best experts on this subject based on the ideXlab platform.
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Role of seaweed Laminaran from Saccharina longicruris on matrix deposition during dermal tissue-engineered production
International journal of biological macromolecules, 2015Co-Authors: Akram Ayoub, Laurieeve Rioux, Sylvie L Turgeon, Martin Beaulieu, Jadson Moreira Pereira, Véronique J. MoulinAbstract:Our laboratory has developed a technique to reconstruct in vitro tissue from human cells using the self-assembly tissue-engineering method, which utilizes the ability of fibroblasts to deposit the matrix they secrete. The time necessary for tissue construction, several weeks, is a drawback for many clinical uses. We hypothesized that the addition of Laminaran can increase the deposition of matrix, speeding up the production of the tissue. Laminaran was isolated from the brown seaweed Saccharina longicruris harvested in Canada and its structure was evaluated. Laminaran is a small molecular weight polysaccharide composed of linear glucose chains. Monolayer-cultured human skin fibroblasts were cultured in the presence of Laminaran with ascorbate for 7 or 35 days to produce a dermis. Treatment did not induce any variation in the growth rate or alpha smooth muscle actin content but it did increase the deposition of collagen I in a dose-dependent manner. After 35 days, the reconstructed dermal thickness was increased when Laminaran was added, and collagen I deposition and MMP activity were also significantly increased. Thus, Laminaran can be used to increase the rate of production of reconstructed self-assembled dermis and can also potentially be used in cosmetic or therapeutic creams to stimulate matrix production.
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structural characterization of Laminaran and galactofucan extracted from the brown seaweed saccharina longicruris
Phytochemistry, 2010Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:Brown seaweed contains several polysaccharides like Laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude Laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The Laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other Laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights <30 kDa. The structure of the galactofucan fractions remained complex after depolymerization, with these also being more sulfated (30-39%) than the crude fractions (13-20%). The crude and RDP fractions contained 3-linked fucopyranose 4-sulfate and 6-linked galactopyranose 3-sulfate moieties, although the galactofucans isolated from M05 and J06 contained less 6-linked galactopyranose 3-sulfate than the A05 and N05 fractions.
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Structural characterization of Laminaran and galactofucan extracted from the brown seaweed Saccharina longicruris.
Phytochemistry, 2010Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:Brown seaweed contains several polysaccharides like Laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude Laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The Laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other Laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights
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effect of season on the composition of bioactive polysaccharides from the brown seaweed saccharina longicruris
Phytochemistry, 2009Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:The structural features of Laminarans and galactofucans extracted from the brown seaweed Saccharina longicruris were determined for four harvest periods (M05, A05, N05 and J06). Crude Laminarans were purified and crude galactofucans were fractionated using DEAE Sepharose anion exchange chromatography with increasing levels of NaCl (0.5, 1 and 2 M). The results showed differences in terms of their monosaccharide compositions. Purified Laminaran contained a high proportion of D-glucose, between 45.1% and 69.1%, with a higher amount in M05 and A05, while the amount of D-mannitol remained constant (less than 1.7%). Crude galactofucans from M05, A05, and N05 contained 19.9-21.5% of sulphates, where J06 had only 14.3%. The 2 M fractionated galactofucans contained a higher proportion of sulphate groups, from 27.1% to 36.9%, for each harvest period, while the 1 M fraction contained 9.2% to 15.9% of sulphates. An important variation in the amount of L-fucose and D-galactose was observed for crude and fractionated galactofucans. In M05, a higher content of L-fucose was observed for crude galactofucans compared to that observed for D-galactose (21.5% vs. 11.1%), whereas the opposite was found for A05 (18.5% vs. 36.6%), N05 (20.9% vs. 36.8%), and J06 (12.8% vs. 19.6%). Also, the 0.5 and 2 M fractions were similar to the crude galactofucans. A05, N05, and J06 contained lower amounts of L-fucose than D-galactose, while the M05 fractions showed the opposite behaviour. However, the 1 M fraction showed a higher amount of L-fucose than D-galactose for each harvest period. The next step will be to study the biological activity of the fractions and to attempt to relate this activity to the structure of the galactofucan and Laminaran fractions.
Tatyana N. Zvyagintseva - One of the best experts on this subject based on the ideXlab platform.
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In vitro anticancer activity of the Laminarans from Far Eastern brown seaweeds and their sulfated derivatives
Journal of Applied Phycology, 2016Co-Authors: Olesya S. Malyarenko, Tatyana N. Zvyagintseva, Roza V. Usoltseva, Natalia M. Shevchenko, Vladimir V. Isakov, Svetlana P. ErmakovaAbstract:Brown seaweeds have drawn worldwide attention due to their involvement in many industrial applications. They produce neutral low molecular weight polysaccharides (Laminarans) with beneficial biological activities. However, the anticancer activity and molecular mechanism of the Laminarans and their sulfated derivatives have not been investigated in detail. Herein, the Laminarans from brown seaweeds Saccharina cichorioides, Saccharina japonica, and Fucus evanescens were isolated. The Laminarans from S. cichorioides and S. japonica were confirmed to contain a main chain of β-(1→3)-D-glucopyranose with single branches at C6. The Laminaran from F. evanescens consisted of not only β-(1→3)-linked D- glucopyranose but also includes single β-(1→6)-linked D-glucose residues. The branches at C6 are presented as glucose or as gentiobiose. The sulfated Laminarans with different degrees of sulfation were obtained by the chlorosulfonic acid-pyridine assay. In modified polysaccharides, the positions of sulfates are directly predetermined by the structure of native Laminarans. The Laminarans and their sulfated derivatives inhibited proliferation, colony formation, and migration of human colorectal adenocarcinoma, melanoma, and breast adenocarcinoma cells in different manners. The sulfated Laminaran from F. evanescens possessed the highest anticancer activity in vitro and effectively prevented migration of breast adenocarcinoma cells by inhibiting of the Matrix Metalloproteinases 2 and 9 activity.
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The composition and structural characteristics of polysaccharides of the brown alga Eisenia bicyclis
Russian Journal of Marine Biology, 2013Co-Authors: Roza V. Menshova, Byung-hun Um, Svetlana P. Ermakova, Tatyana N. ZvyagintsevaAbstract:The present study examines the polysaccharide composition of the brown alga Eisenia bicyclis. The major polysaccharide of E. bicyclis is shown to be sodium alginate (15.8% of the dry defatted alga weight). The yields of Laminaran and fucoidan were 1.4% and 1.3% of the dry defatted alga weight, respectively. The alga contains Laminaran (1,3;1,6-β-D-glucan) that has a unique structure, a high content of 1,6-linked D-glucose residues (the ratio of 1,3: 1,6 linkage is 1.5: 1), and an unusually high molecular weight (19–27 kDa). The fucoidan fractions isolated from E. bicyclis are sulfated and acetylated heteropolysaccharides that differ in their sulfate content and monosaccharide composition.
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Water-soluble polysaccharides from the brown alga Eisenia bicyclis: Structural characteristics and antitumor activity
Algal Research, 2013Co-Authors: Svetlana P. Ermakova, Roza V. Menshova, Vladimir V. Isakov, Olesya S. Vishchuk, Sang Min Kim, Tatyana N. ZvyagintsevaAbstract:Abstract Water-soluble polysaccharides were isolated from the brown alga Eisenia bicyclis , which was collected near the coast of the Republic of Korea. The structures of Laminaran and fucoidan were investigated. Laminaran from E. bicyclis was determined to be a glucan with β-(1 → 6) side chains linked to a β-(1 → 3) backbone with relatively few branch points. Based on nuclear magnetic resonance (NMR) data, the ratio of the β-(1 → 3) and β-(1 → 6) linkages was estimated as 2.6:1. Fucoidan from E. bicyclis was found to contain 1,3-linked fucose residues, some 1,6-, 1,2,6-, 1,4,6-linked galactose residues and traces of mannose and xylose. In addition, the amount of sulfate in fucoidan was 13.2%. Those polysaccharides were non-cytotoxic to human melanoma SK-MEL-28 and colon cancer DLD-1 cells. Laminaran and fucoidan from E. bicyclis inhibited the colony formation of those cells. Therefore, they may have potential as antitumor agents.
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An endo-(1-->3)-beta-D-glucanase from the scallop Chlamys albidus: catalytic properties, cDNA cloning and secondary-structure characterization.
Carbohydrate research, 2008Co-Authors: Svetlana N. Kovalchuk, Irina Y. Bakunina, Yulia V. Burtseva, Viktor I. Emelyanenko, Natalia Yu. Kim, Konstantin V. Guzev, Valeri B. Kozhemyako, Valeri A. Rasskazov, Tatyana N. ZvyagintsevaAbstract:An endo-(1-->3)-beta-d-glucanase (L(0)) with molecular mass of 37 kDa was purified to homogeneity from the crystalline style of the scallop Chlamys albidus. The endo-(1-->3)-beta-d-glucanase was extremely thermolabile with a half-life of 10 min at 37 degrees C. L(0) hydrolyzed Laminaran with K(m) approximately 0.75 mg/mL, and catalyzed effectively transglycosylation reactions with Laminaran as donor and p-nitrophenyl betad-glucoside as acceptor (K(m) approximately 2mg/mL for Laminaran) and Laminaran as donor and as acceptor (K(m) approximately 5mg/mL) yielding p-nitrophenyl betad-glucooligosaccharides (n=2-6) and high-molecular branching (1-->3),(1-->6)-beta-d-glucans, respectively. Efficiency of hydrolysis and transglycosylation processes depended on the substrate structure and decreased appreciably with the increase of the percentage of beta-(1-->6)-glycosidic bonds, and Laminaran with 10% of beta-(1-->6)-glycosidic bonds was the optimal substrate for both reactions. The CD spectrum of L(0) was characteristic for a protein with prevailing beta secondary-structural elements. Binding L(0) with d-glucose as the best acceptor for transglycosylation was investigated by the methods of intrinsic tryptophan fluorescence and CD. Glucose in concentration sufficient to saturate the enzyme binding sites resulted in a red shift in the maximum of fluorescence emission of 1-1.5 nm and quenching the Trp fluorescence up to 50%. An apparent association constant of L(0) with glucose (K(a)=7.4 x 10(5)+/-1.1 x 10(5)M(-1)) and stoichiometry (n=13.3+/-0.7) was calculated. The cDNA encoding L(0) was sequenced, and the enzyme was classified in glycoside hydrolases family 16 on the basis of the amino acid sequence similarity.
Olesya S. Malyarenko - One of the best experts on this subject based on the ideXlab platform.
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Aminated Laminaran from brown alga Saccharina cichorioides: Synthesis, structure, anticancer, and radiosensitizing potential in vitro
Carbohydrate polymers, 2020Co-Authors: Olesya S. Malyarenko, Roza V. Usoltseva, Artem S. Silchenko, Svetlana P. ErmakovaAbstract:Abstract Laminarans are currently the focus of attention in regard to the selection of prospective agents for the prevention and treatment of cancer. Laminaran from Saccharina cichorioides was aminated to heighten anticancer and radiosensitizing activities and elucidate its molecular mode of action. Aminated Laminaran, ScLNH2, was identified as 1,3-β- d -glucan with −CH2−CH(OH)−CH2-NH2 group at the C6 of branches. ScLNH2 selectively inhibited the viability and colony formation in the MDA-MB-231 cell line of triple negative breast cancer cells. ScLNH2 possessed synergism with radiation, resulting in a decreased number of colonies of MDA-MB-231 cells. The mechanism underling the radiosensitizing effect of ScLNH2 was associated with apoptosis induction via regulation of caspases 9 and 3 and PARP enzyme, preventing the repair of DNA damage in irradiated cells. These findings confirmed that combination therapy by aminated Laminaran and radiation might play a role in the optimization of therapy for an aggressive form of human breast cancer.
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Laminarans and 1,3-β-D-glucanases.
International journal of biological macromolecules, 2020Co-Authors: Roza V. Usoltseva, Olesya S. Malyarenko, Mikhail I. Kusaykin, Aleksei A. Belik, Tatiana N. Zvyagintsevа, Svetlana P. ErmakovaAbstract:The Laminarans are biologically active water-soluble polysaccharide (1,3;1,6-β-D-glucans) of brown algae. These polysaccharides are an attractive object for research due to its relatively simple structure, low toxicity, and various biological effects. 1,3-β-D-glucanases are an effective tool for studying the structure of Laminarans, and can also be used to obtain new biologically active derivatives. This review is to outline what is currently known about Laminarans and enzymes that catalyze of their transformation. We focused on information about sources, structure and properties of Laminarans and 1,3-β-D-glucanases, methods of obtaining and structural elucidation of Laminarans, and biological activity of Laminarans and products of their enzymatic transformation. It has an increased focus on the immunomodulating and anticancer activity of Laminarans and their derivatives.
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Laminaran from brown alga dictyota dichotoma and its sulfated derivative as radioprotectors and radiosensitizers in melanoma therapy
Carbohydrate Polymers, 2019Co-Authors: Olesya S. Malyarenko, Roza V. Usoltseva, T N Zvyagintseva, Svetlana P. ErmakovaAbstract:Abstract The Laminarans are neutral water-soluble β-D-glucans of brown algae possessing potent immunomodulating, radioprotective, and anticancer activities. The aim of the present study was to investigate in vitro anticancer, radioprotective, and radiosensitizing activities of Laminaran from brown alga Dictyota dichotoma and its sulfated derivative. The native and sulfated Laminarans by themselves at non-toxic doses possessed significant anticancer activity against melanoma cells. Both polysaccharides protected normal epidermal cells, while only sulfated Laminaran was able to sensitize melanoma cells to X-rays irradiation resulting in significant inhibition of cell proliferation, colony formation, and migration of cancer cells. The molecular mechanism of this action was related to the inhibition of MMP-2 and MMP-9 proteinases activity as well as down-regulation of kinases’ phosphorylation of ERK1/2 signaling cascade. Taken together, the combination of sulfated derivative of Laminaran from D. dichotoma with X-ray may serve as a potential treatment strategy for human melanoma.
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In vitro anticancer activity of the Laminarans from Far Eastern brown seaweeds and their sulfated derivatives
Journal of Applied Phycology, 2016Co-Authors: Olesya S. Malyarenko, Tatyana N. Zvyagintseva, Roza V. Usoltseva, Natalia M. Shevchenko, Vladimir V. Isakov, Svetlana P. ErmakovaAbstract:Brown seaweeds have drawn worldwide attention due to their involvement in many industrial applications. They produce neutral low molecular weight polysaccharides (Laminarans) with beneficial biological activities. However, the anticancer activity and molecular mechanism of the Laminarans and their sulfated derivatives have not been investigated in detail. Herein, the Laminarans from brown seaweeds Saccharina cichorioides, Saccharina japonica, and Fucus evanescens were isolated. The Laminarans from S. cichorioides and S. japonica were confirmed to contain a main chain of β-(1→3)-D-glucopyranose with single branches at C6. The Laminaran from F. evanescens consisted of not only β-(1→3)-linked D- glucopyranose but also includes single β-(1→6)-linked D-glucose residues. The branches at C6 are presented as glucose or as gentiobiose. The sulfated Laminarans with different degrees of sulfation were obtained by the chlorosulfonic acid-pyridine assay. In modified polysaccharides, the positions of sulfates are directly predetermined by the structure of native Laminarans. The Laminarans and their sulfated derivatives inhibited proliferation, colony formation, and migration of human colorectal adenocarcinoma, melanoma, and breast adenocarcinoma cells in different manners. The sulfated Laminaran from F. evanescens possessed the highest anticancer activity in vitro and effectively prevented migration of breast adenocarcinoma cells by inhibiting of the Matrix Metalloproteinases 2 and 9 activity.
Laurieeve Rioux - One of the best experts on this subject based on the ideXlab platform.
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Role of seaweed Laminaran from Saccharina longicruris on matrix deposition during dermal tissue-engineered production
International journal of biological macromolecules, 2015Co-Authors: Akram Ayoub, Laurieeve Rioux, Sylvie L Turgeon, Martin Beaulieu, Jadson Moreira Pereira, Véronique J. MoulinAbstract:Our laboratory has developed a technique to reconstruct in vitro tissue from human cells using the self-assembly tissue-engineering method, which utilizes the ability of fibroblasts to deposit the matrix they secrete. The time necessary for tissue construction, several weeks, is a drawback for many clinical uses. We hypothesized that the addition of Laminaran can increase the deposition of matrix, speeding up the production of the tissue. Laminaran was isolated from the brown seaweed Saccharina longicruris harvested in Canada and its structure was evaluated. Laminaran is a small molecular weight polysaccharide composed of linear glucose chains. Monolayer-cultured human skin fibroblasts were cultured in the presence of Laminaran with ascorbate for 7 or 35 days to produce a dermis. Treatment did not induce any variation in the growth rate or alpha smooth muscle actin content but it did increase the deposition of collagen I in a dose-dependent manner. After 35 days, the reconstructed dermal thickness was increased when Laminaran was added, and collagen I deposition and MMP activity were also significantly increased. Thus, Laminaran can be used to increase the rate of production of reconstructed self-assembled dermis and can also potentially be used in cosmetic or therapeutic creams to stimulate matrix production.
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structural characterization of Laminaran and galactofucan extracted from the brown seaweed saccharina longicruris
Phytochemistry, 2010Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:Brown seaweed contains several polysaccharides like Laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude Laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The Laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other Laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights <30 kDa. The structure of the galactofucan fractions remained complex after depolymerization, with these also being more sulfated (30-39%) than the crude fractions (13-20%). The crude and RDP fractions contained 3-linked fucopyranose 4-sulfate and 6-linked galactopyranose 3-sulfate moieties, although the galactofucans isolated from M05 and J06 contained less 6-linked galactopyranose 3-sulfate than the A05 and N05 fractions.
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Structural characterization of Laminaran and galactofucan extracted from the brown seaweed Saccharina longicruris.
Phytochemistry, 2010Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:Brown seaweed contains several polysaccharides like Laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude Laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The Laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other Laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights
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effect of season on the composition of bioactive polysaccharides from the brown seaweed saccharina longicruris
Phytochemistry, 2009Co-Authors: Laurieeve Rioux, Sylvie L Turgeon, Martin BeaulieuAbstract:The structural features of Laminarans and galactofucans extracted from the brown seaweed Saccharina longicruris were determined for four harvest periods (M05, A05, N05 and J06). Crude Laminarans were purified and crude galactofucans were fractionated using DEAE Sepharose anion exchange chromatography with increasing levels of NaCl (0.5, 1 and 2 M). The results showed differences in terms of their monosaccharide compositions. Purified Laminaran contained a high proportion of D-glucose, between 45.1% and 69.1%, with a higher amount in M05 and A05, while the amount of D-mannitol remained constant (less than 1.7%). Crude galactofucans from M05, A05, and N05 contained 19.9-21.5% of sulphates, where J06 had only 14.3%. The 2 M fractionated galactofucans contained a higher proportion of sulphate groups, from 27.1% to 36.9%, for each harvest period, while the 1 M fraction contained 9.2% to 15.9% of sulphates. An important variation in the amount of L-fucose and D-galactose was observed for crude and fractionated galactofucans. In M05, a higher content of L-fucose was observed for crude galactofucans compared to that observed for D-galactose (21.5% vs. 11.1%), whereas the opposite was found for A05 (18.5% vs. 36.6%), N05 (20.9% vs. 36.8%), and J06 (12.8% vs. 19.6%). Also, the 0.5 and 2 M fractions were similar to the crude galactofucans. A05, N05, and J06 contained lower amounts of L-fucose than D-galactose, while the M05 fractions showed the opposite behaviour. However, the 1 M fraction showed a higher amount of L-fucose than D-galactose for each harvest period. The next step will be to study the biological activity of the fractions and to attempt to relate this activity to the structure of the galactofucan and Laminaran fractions.
