The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform
Massimiliano Valeriani - One of the best experts on this subject based on the ideXlab platform.
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Homotopic reduction in Laser-Evoked Potential amplitude and Laser-pain rating by abdominal acupuncture.
European journal of pain (London England), 2020Co-Authors: Massimiliano Valeriani, Catello Vollono, Stefano Liguori, E. Testani, Sergio Bangrazi, Filomena Petti, Aldo Liguori, Marco Germanotta, Luca Padua, Costanza PazzagliaAbstract:BACKGROUND The neural mechanism underlying the analgesic effect of acupuncture is largely unknown. We aimed at investigating the effect of abdominal acupuncture (AA) on the Laser-Evoked Potential (LEP) amplitude and Laser-pain rating to stimulation of body parts either homotopic or heterotopic to the treated acupoint. METHODS Laser-Evoked Potentials were recorded from 13 healthy subjects to stimulation of the right wrist (RW), left wrist (LW) and right foot (RF). LEPs were obtained before, during and after the AA stimulation of an abdominal area corresponding to the representation of the RW. Subjective Laser-pain rating was collected after each LEP recording. RESULTS The amplitude of the N2/P2 LEP component was significantly reduced during AA and 15 min after needle removal to both RW (F = 4.14, p = .02) and LW (F = 5.48, p = .008) stimulation, while the N2/P2 amplitude to RF stimulation (F = 0.94, p = .4) remained unchanged. Laser-pain rating was reduced during AA and 15 min after needle removal only to RW stimulation (F = 5.67, p = .007). CONCLUSION Our findings showing an AA effect on LEP components to both the ipsilateral and contralateral region homotopic to the treated area, without any LEP change to stimulation of a heterotopic region, suggest that the AA analgesia is mediated by a segmental spinal mechanism. SIGNIFICANCE Although abdominal acupuncture has demonstrated to be effective in the reduction in Laser-Evoked Potential (LEP) amplitude and Laser-pain rating, the exact mechanism of this analgesic effect is not known. In the current study, we found that treatment of an area in the "turtle representation" of the body led to a topographical pattern of LEP amplitude inhibition that can be mediated by a segmental spinal mechanism.
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znrd1 as and rp11 819c21 1 long non coding rna changes following painful Laser stimulation correlate with Laser Evoked Potential amplitude and habituation in healthy subjects a pilot study
European Journal of Pain, 2020Co-Authors: Massimo Santoro, Costanza Pazzaglia, Catello Vollono, Luca Padua, Rocco Giordano, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:BACKGROUND Non-coding RNAs (lncRNAs) are a group of non-coding RNAs that act as regulators of gene expression; they are implicated in various human diseases and have been reported to be involved in the modulation of pain. We aimed to study whether: (a) lncRNAs modifications could be found in an experimental model of pain; (b) there was a correlation between lncRNA changes and Laser Evoked Potential (LEP) amplitude/Laser-pain rating. METHODS Laser Evoked Potentials were recorded from 11 healthy subjects to both left hand and perioral region stimulation. Three consecutive averages were calculated for each stimulation site in order to investigate the LEP amplitude habituation. Blood samples were obtained immediately before LEP recording (pre-pain) and 30-min after the recording of the last LEP average (post-pain). Eighty-four lncRNAs, involved in autoimmunity and human inflammatory response, were screened. The criteria used for lncRNAs analysis were fold change >2 and p < .05. By Real-Time PCR, we identified two lncRNAs up-regulated at the post-pain time, as compared to the pre-pain time: RP11-819C21.1 (fold change = 8.2; p = .038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS; fold change = 6.3; p = .037). RESULTS The ZNRD1-AS up-regulation was directly correlated with the N1 amplitude, while the RP11-819C21.1 increase after pain showed a correlation with the reduced N2/P2 amplitude and Laser-pain habituation. CONCLUSION IncRNA changes in a human experimental phasic pain model. The correlation between lncRNA changes and LEP amplitude and habituation suggests that RP11-819C21.1 and ZNRD1-AS could be involved in the pathophysiology of painful diseases characterized by abnormal excitability of the cerebral cortex. SIGNIFICANCE Long non-coding RNAs are upregulated after experimental pain. RP11-819C21.1 and ZNRD1 could be involved in the pathophysiology of diseases characterized by reduced habituation to pain.
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p46 s rp11 819c21 1 and znrd1 as long non coding rna changes following experimental pain correlate with Laser Evoked Potential habituation
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Costanza Pazzaglia, Luca Padua, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:Background Long non-coding RNAs (lncRNAs) are a group of non-coding RNAs acting as regulators of gene expression, through interaction with histones or through interaction with complementary DNA sequences, implicated in various human diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders and have been reported to be involved in the modulation of neuropathic pain. We recorded Laser Evoked Potentials (LEPs) in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. Material and methods LEPs were recorded in 11 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were collected immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Results We identified 2 lncRNAs up-regulated at the post-pain time: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). The up-regulation of both lncRNAs showed a significant positive correlation with the LEP habituation to perioral region stimulation (p = 0.04 and p = 0.01, respectively). Conclusions This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a and miR19b, a class of microRNAs involved in modulation of multiple potassium channel α-subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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rp11 819c21 1 and znrd1 as long non coding rna changes following painful Laser stimulation correlate with pain habituation in healthy subjects a Laser Evoked Potential lep study
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, C Pazzaglia, L Padua, Massimiliano ValerianiAbstract:Long non-coding RNAs (lncRNAs) are a group of 200 nucleotides acting as regulators of gene expression implicated in various diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders. Moreover, lncRNAs are involved in pain modulation. We recorded LEPs in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. LEPs were recorded in 12 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were obtained immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Two lncRNAs were up-regulated after pain: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense-RNA1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). lncRNAs up-regulated showed a significant positive correlation with perioral LEP habituation (p = 0.04 and p = 0.01, respectively). This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a andmiR19b, a class of microRNAs involved in modulation of multiple potassium channel α -subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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Laser Evoked Potential amplitude and Laser-pain rating reduction during high-frequency non-noxious somatosensory stimulation
Clinical Neurophysiology, 2018Co-Authors: Massimiliano Valeriani, Vincenzo Rizzo, Costanza Pazzaglia, Angelo Quartarone, Catello VollonoAbstract:Abstract Objective To investigate the mechanism subtending the analgesic effect of high frequency non-painful somatosensory stimulation. Methods Laser Evoked Potentials (LEPs) and Laser-pain rating were obtained from healthy subjects to stimulation of different parts of the body. LEPs were recorded at baseline and during non-painful electrical stimulation of the superficial branch of the right radial nerve (RRES). Results RRES reduced N2/P2 LEP amplitude to right radial (F(8,10) = 82.4, p 0.05). Conclusions Our study confirms that the non-nociceptive afferents dampen the nociceptive input. The spatial pattern of this interaction suggests that, when conditioning higher frequency non-painful stimulation is used, the inhibition takes place at the spinal cord. Significance Our experimental design reproduces what happens when non-painful somatosensory stimuli are used to reduce pain, such as rubbing a wound or during transcutaneous electrical nerve stimulation. Therefore, in these situations the analgesia is likely to occur at the spinal cord level.
Catello Vollono - One of the best experts on this subject based on the ideXlab platform.
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Homotopic reduction in Laser-Evoked Potential amplitude and Laser-pain rating by abdominal acupuncture.
European journal of pain (London England), 2020Co-Authors: Massimiliano Valeriani, Catello Vollono, Stefano Liguori, E. Testani, Sergio Bangrazi, Filomena Petti, Aldo Liguori, Marco Germanotta, Luca Padua, Costanza PazzagliaAbstract:BACKGROUND The neural mechanism underlying the analgesic effect of acupuncture is largely unknown. We aimed at investigating the effect of abdominal acupuncture (AA) on the Laser-Evoked Potential (LEP) amplitude and Laser-pain rating to stimulation of body parts either homotopic or heterotopic to the treated acupoint. METHODS Laser-Evoked Potentials were recorded from 13 healthy subjects to stimulation of the right wrist (RW), left wrist (LW) and right foot (RF). LEPs were obtained before, during and after the AA stimulation of an abdominal area corresponding to the representation of the RW. Subjective Laser-pain rating was collected after each LEP recording. RESULTS The amplitude of the N2/P2 LEP component was significantly reduced during AA and 15 min after needle removal to both RW (F = 4.14, p = .02) and LW (F = 5.48, p = .008) stimulation, while the N2/P2 amplitude to RF stimulation (F = 0.94, p = .4) remained unchanged. Laser-pain rating was reduced during AA and 15 min after needle removal only to RW stimulation (F = 5.67, p = .007). CONCLUSION Our findings showing an AA effect on LEP components to both the ipsilateral and contralateral region homotopic to the treated area, without any LEP change to stimulation of a heterotopic region, suggest that the AA analgesia is mediated by a segmental spinal mechanism. SIGNIFICANCE Although abdominal acupuncture has demonstrated to be effective in the reduction in Laser-Evoked Potential (LEP) amplitude and Laser-pain rating, the exact mechanism of this analgesic effect is not known. In the current study, we found that treatment of an area in the "turtle representation" of the body led to a topographical pattern of LEP amplitude inhibition that can be mediated by a segmental spinal mechanism.
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znrd1 as and rp11 819c21 1 long non coding rna changes following painful Laser stimulation correlate with Laser Evoked Potential amplitude and habituation in healthy subjects a pilot study
European Journal of Pain, 2020Co-Authors: Massimo Santoro, Costanza Pazzaglia, Catello Vollono, Luca Padua, Rocco Giordano, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:BACKGROUND Non-coding RNAs (lncRNAs) are a group of non-coding RNAs that act as regulators of gene expression; they are implicated in various human diseases and have been reported to be involved in the modulation of pain. We aimed to study whether: (a) lncRNAs modifications could be found in an experimental model of pain; (b) there was a correlation between lncRNA changes and Laser Evoked Potential (LEP) amplitude/Laser-pain rating. METHODS Laser Evoked Potentials were recorded from 11 healthy subjects to both left hand and perioral region stimulation. Three consecutive averages were calculated for each stimulation site in order to investigate the LEP amplitude habituation. Blood samples were obtained immediately before LEP recording (pre-pain) and 30-min after the recording of the last LEP average (post-pain). Eighty-four lncRNAs, involved in autoimmunity and human inflammatory response, were screened. The criteria used for lncRNAs analysis were fold change >2 and p < .05. By Real-Time PCR, we identified two lncRNAs up-regulated at the post-pain time, as compared to the pre-pain time: RP11-819C21.1 (fold change = 8.2; p = .038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS; fold change = 6.3; p = .037). RESULTS The ZNRD1-AS up-regulation was directly correlated with the N1 amplitude, while the RP11-819C21.1 increase after pain showed a correlation with the reduced N2/P2 amplitude and Laser-pain habituation. CONCLUSION IncRNA changes in a human experimental phasic pain model. The correlation between lncRNA changes and LEP amplitude and habituation suggests that RP11-819C21.1 and ZNRD1-AS could be involved in the pathophysiology of painful diseases characterized by abnormal excitability of the cerebral cortex. SIGNIFICANCE Long non-coding RNAs are upregulated after experimental pain. RP11-819C21.1 and ZNRD1 could be involved in the pathophysiology of diseases characterized by reduced habituation to pain.
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p46 s rp11 819c21 1 and znrd1 as long non coding rna changes following experimental pain correlate with Laser Evoked Potential habituation
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Costanza Pazzaglia, Luca Padua, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:Background Long non-coding RNAs (lncRNAs) are a group of non-coding RNAs acting as regulators of gene expression, through interaction with histones or through interaction with complementary DNA sequences, implicated in various human diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders and have been reported to be involved in the modulation of neuropathic pain. We recorded Laser Evoked Potentials (LEPs) in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. Material and methods LEPs were recorded in 11 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were collected immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Results We identified 2 lncRNAs up-regulated at the post-pain time: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). The up-regulation of both lncRNAs showed a significant positive correlation with the LEP habituation to perioral region stimulation (p = 0.04 and p = 0.01, respectively). Conclusions This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a and miR19b, a class of microRNAs involved in modulation of multiple potassium channel α-subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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rp11 819c21 1 and znrd1 as long non coding rna changes following painful Laser stimulation correlate with pain habituation in healthy subjects a Laser Evoked Potential lep study
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, C Pazzaglia, L Padua, Massimiliano ValerianiAbstract:Long non-coding RNAs (lncRNAs) are a group of 200 nucleotides acting as regulators of gene expression implicated in various diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders. Moreover, lncRNAs are involved in pain modulation. We recorded LEPs in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. LEPs were recorded in 12 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were obtained immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Two lncRNAs were up-regulated after pain: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense-RNA1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). lncRNAs up-regulated showed a significant positive correlation with perioral LEP habituation (p = 0.04 and p = 0.01, respectively). This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a andmiR19b, a class of microRNAs involved in modulation of multiple potassium channel α -subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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Laser Evoked Potential amplitude and Laser-pain rating reduction during high-frequency non-noxious somatosensory stimulation
Clinical Neurophysiology, 2018Co-Authors: Massimiliano Valeriani, Vincenzo Rizzo, Costanza Pazzaglia, Angelo Quartarone, Catello VollonoAbstract:Abstract Objective To investigate the mechanism subtending the analgesic effect of high frequency non-painful somatosensory stimulation. Methods Laser Evoked Potentials (LEPs) and Laser-pain rating were obtained from healthy subjects to stimulation of different parts of the body. LEPs were recorded at baseline and during non-painful electrical stimulation of the superficial branch of the right radial nerve (RRES). Results RRES reduced N2/P2 LEP amplitude to right radial (F(8,10) = 82.4, p 0.05). Conclusions Our study confirms that the non-nociceptive afferents dampen the nociceptive input. The spatial pattern of this interaction suggests that, when conditioning higher frequency non-painful stimulation is used, the inhibition takes place at the spinal cord. Significance Our experimental design reproduces what happens when non-painful somatosensory stimuli are used to reduce pain, such as rubbing a wound or during transcutaneous electrical nerve stimulation. Therefore, in these situations the analgesia is likely to occur at the spinal cord level.
Costanza Pazzaglia - One of the best experts on this subject based on the ideXlab platform.
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Homotopic reduction in Laser-Evoked Potential amplitude and Laser-pain rating by abdominal acupuncture.
European journal of pain (London England), 2020Co-Authors: Massimiliano Valeriani, Catello Vollono, Stefano Liguori, E. Testani, Sergio Bangrazi, Filomena Petti, Aldo Liguori, Marco Germanotta, Luca Padua, Costanza PazzagliaAbstract:BACKGROUND The neural mechanism underlying the analgesic effect of acupuncture is largely unknown. We aimed at investigating the effect of abdominal acupuncture (AA) on the Laser-Evoked Potential (LEP) amplitude and Laser-pain rating to stimulation of body parts either homotopic or heterotopic to the treated acupoint. METHODS Laser-Evoked Potentials were recorded from 13 healthy subjects to stimulation of the right wrist (RW), left wrist (LW) and right foot (RF). LEPs were obtained before, during and after the AA stimulation of an abdominal area corresponding to the representation of the RW. Subjective Laser-pain rating was collected after each LEP recording. RESULTS The amplitude of the N2/P2 LEP component was significantly reduced during AA and 15 min after needle removal to both RW (F = 4.14, p = .02) and LW (F = 5.48, p = .008) stimulation, while the N2/P2 amplitude to RF stimulation (F = 0.94, p = .4) remained unchanged. Laser-pain rating was reduced during AA and 15 min after needle removal only to RW stimulation (F = 5.67, p = .007). CONCLUSION Our findings showing an AA effect on LEP components to both the ipsilateral and contralateral region homotopic to the treated area, without any LEP change to stimulation of a heterotopic region, suggest that the AA analgesia is mediated by a segmental spinal mechanism. SIGNIFICANCE Although abdominal acupuncture has demonstrated to be effective in the reduction in Laser-Evoked Potential (LEP) amplitude and Laser-pain rating, the exact mechanism of this analgesic effect is not known. In the current study, we found that treatment of an area in the "turtle representation" of the body led to a topographical pattern of LEP amplitude inhibition that can be mediated by a segmental spinal mechanism.
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znrd1 as and rp11 819c21 1 long non coding rna changes following painful Laser stimulation correlate with Laser Evoked Potential amplitude and habituation in healthy subjects a pilot study
European Journal of Pain, 2020Co-Authors: Massimo Santoro, Costanza Pazzaglia, Catello Vollono, Luca Padua, Rocco Giordano, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:BACKGROUND Non-coding RNAs (lncRNAs) are a group of non-coding RNAs that act as regulators of gene expression; they are implicated in various human diseases and have been reported to be involved in the modulation of pain. We aimed to study whether: (a) lncRNAs modifications could be found in an experimental model of pain; (b) there was a correlation between lncRNA changes and Laser Evoked Potential (LEP) amplitude/Laser-pain rating. METHODS Laser Evoked Potentials were recorded from 11 healthy subjects to both left hand and perioral region stimulation. Three consecutive averages were calculated for each stimulation site in order to investigate the LEP amplitude habituation. Blood samples were obtained immediately before LEP recording (pre-pain) and 30-min after the recording of the last LEP average (post-pain). Eighty-four lncRNAs, involved in autoimmunity and human inflammatory response, were screened. The criteria used for lncRNAs analysis were fold change >2 and p < .05. By Real-Time PCR, we identified two lncRNAs up-regulated at the post-pain time, as compared to the pre-pain time: RP11-819C21.1 (fold change = 8.2; p = .038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS; fold change = 6.3; p = .037). RESULTS The ZNRD1-AS up-regulation was directly correlated with the N1 amplitude, while the RP11-819C21.1 increase after pain showed a correlation with the reduced N2/P2 amplitude and Laser-pain habituation. CONCLUSION IncRNA changes in a human experimental phasic pain model. The correlation between lncRNA changes and LEP amplitude and habituation suggests that RP11-819C21.1 and ZNRD1-AS could be involved in the pathophysiology of painful diseases characterized by abnormal excitability of the cerebral cortex. SIGNIFICANCE Long non-coding RNAs are upregulated after experimental pain. RP11-819C21.1 and ZNRD1 could be involved in the pathophysiology of diseases characterized by reduced habituation to pain.
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p46 s rp11 819c21 1 and znrd1 as long non coding rna changes following experimental pain correlate with Laser Evoked Potential habituation
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Costanza Pazzaglia, Luca Padua, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:Background Long non-coding RNAs (lncRNAs) are a group of non-coding RNAs acting as regulators of gene expression, through interaction with histones or through interaction with complementary DNA sequences, implicated in various human diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders and have been reported to be involved in the modulation of neuropathic pain. We recorded Laser Evoked Potentials (LEPs) in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. Material and methods LEPs were recorded in 11 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were collected immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Results We identified 2 lncRNAs up-regulated at the post-pain time: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). The up-regulation of both lncRNAs showed a significant positive correlation with the LEP habituation to perioral region stimulation (p = 0.04 and p = 0.01, respectively). Conclusions This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a and miR19b, a class of microRNAs involved in modulation of multiple potassium channel α-subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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Laser Evoked Potential amplitude and Laser-pain rating reduction during high-frequency non-noxious somatosensory stimulation
Clinical Neurophysiology, 2018Co-Authors: Massimiliano Valeriani, Vincenzo Rizzo, Costanza Pazzaglia, Angelo Quartarone, Catello VollonoAbstract:Abstract Objective To investigate the mechanism subtending the analgesic effect of high frequency non-painful somatosensory stimulation. Methods Laser Evoked Potentials (LEPs) and Laser-pain rating were obtained from healthy subjects to stimulation of different parts of the body. LEPs were recorded at baseline and during non-painful electrical stimulation of the superficial branch of the right radial nerve (RRES). Results RRES reduced N2/P2 LEP amplitude to right radial (F(8,10) = 82.4, p 0.05). Conclusions Our study confirms that the non-nociceptive afferents dampen the nociceptive input. The spatial pattern of this interaction suggests that, when conditioning higher frequency non-painful stimulation is used, the inhibition takes place at the spinal cord. Significance Our experimental design reproduces what happens when non-painful somatosensory stimuli are used to reduce pain, such as rubbing a wound or during transcutaneous electrical nerve stimulation. Therefore, in these situations the analgesia is likely to occur at the spinal cord level.
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P247 Painful Laser Evoked Potential inhibition during high-frequency non-noxious somatosensory stimulation
Clinical Neurophysiology, 2017Co-Authors: Massimiliano Valeriani, Vincenzo Rizzo, Costanza Pazzaglia, Angelo Quartarone, Catello VollonoAbstract:Objectives The inhibition of single nociceptive inputs by single non-painful stimuli was shown to occur at supraspinal level (Testani et al., 2015). However, the neurophysiological mechanism subserving the analgesia induced by rubbing the painful part of the body or during TENS is still unknown. Our aim was to investigate the site of this inhibition. Methods We studied 10 healthy volunteers. LEPs were recorded after stimulation of the radial and ulnar territories of both the right and left hand dorsum in 2 conditions: (1) no conditioning stimulation (baseline condition), and (2) high-frequency (5 Hz) non-painful electrical stimulation of the right radial nerve (gating condition). Results As compared to the baseline, in the gating condition the N2/P2 amplitude was reduced in amplitude after stimulation of the radial territory of both hands ( p 0.001 and p 0.001 for right and left hand, respectively) and of the right ulnar region (p = 0.008), while no inhibition was found to left ulnar region stimulation (p = 0.06). Discussion Our results show that high-frequency non-painful stimulation of the right radial nerve inhibits the nociceptive input coming from both the ispilateral and contralateral homotopic regions, and from a close ispilateral heterotopic territory. On the contrary, it does not have any effect on the nociceptive input due to stimulation of a contralateral heterotopic area. Conclusion Spinal segmental mechanisms are mainly involved in the nociceptive input inhibition by high-frequency non-noxious somatosensory stimulation. Significance Pain inhibition induced by high-frequency somatosensory stimulation, such as TENS, is likely to occur at the spinal cord level.
Anthony K. P. Jones - One of the best experts on this subject based on the ideXlab platform.
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Negative expectations interfere with the analgesic effect of safety cues on pain perception by priming the cortical representation of pain in the midcingulate cortex
2017Co-Authors: Abeer F. Almarzouki, Richard J Brown, Christopher A Brown, Matthew H. K. Leung, Anthony K. P. JonesAbstract:It is well known that the efficacy of treatment effects, including those of placebos, is heavily dependent on positive expectations regarding treatment outcomes. For example, positive expectations about pain treatments are essential for pain reduction. Such positive expectations not only depend on the properties of the treatment itself, but also on the context in which the treatment is presented. However, it is not clear how the preceding threat of pain will bias positive expectancy effects. One hypothesis is that threatening contexts trigger fearful and catastrophic thinking, reducing the pain-relieving effects of positive expectancy. In this study, we investigated the disruptive influence of threatening contexts on positive expectancy effects while 41 healthy volunteers experienced Laser-induced heat pain. A threatening context was induced using pain-threatening cues that preceded the induction of positive expectancies via subsequent pain-safety cues. We also utilised electroencephalography (EEG) to investigate Potential neural mechanisms underlying these effects. Lastly, we used the Fear of Pain Questionnaire to address whether the disruptive effect of negative contexts on cued pain relief was related to the degree of fear of pain. As predicted, participants responded less to pain-safety cues (i.e., experienced more pain) when these were preceded by pain-threatening cues. In this threatening context, an enhancement of the N2 component of the Laser-Evoked Potential was detected, which was more pronounced in fearful individuals. This effect was localised to the midcingulate cortex, an area thought to integrate negative affect with pain experience to enable adaptive behaviour in aversive situations. These results suggest that threatening contexts disrupt the effect of pain relief cues via an aversive priming mechanism that enhances neural responses in the early stages of sensory processing.
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some words hurt more than others semantic activation of pain concepts in memory and subsequent experiences of pain
Pain, 2015Co-Authors: Ellen R Swannell, Anthony K. P. Jones, Christopher A Brown, Richard J BrownAbstract:Theory suggests that as activation of pain concepts in memory increases, so too does sub- sequent pain perception. Previously, researchers have found that activating pain concepts in memory increases pain perception of subsequent painful stimuli, relative to neutral information. However, they have not attempted to quantify the nature of the association between information studied and ensuing pain perception. We subliminally presented words that had either a low or high degree of association to the word 'pain,' although this was only partially successful and some words were consciously perceived. Participants then received randomized Laser heat stimuli, delivered at 1 of 3 intensity levels (low, moderate, high), and we measured the effect of this on behavioral and electro- physiological measures of pain. Participants (N = 27) rated moderate- and high-intensity Laser stimuli as more painful after viewing high relative to low associates of pain; these effects remained present when we controlled for measures of mood, anxiety, and physical symptom reporting. Similar effects were observed physiologically, with higher stimulus negativity preceding after high relative to low associates and greater amplitudes for the N2 component of the Laser-Evoked Potential after presen- tation of high associates in the moderate and high Laser intensity conditions. These data support activation-based models of the effects of memory on pain perception. Perspective: Consistent with current theories of memory and pain, we found that high, relative to low activation of pain concepts in memory increased psychological and physiological responses to Laser-induced pain. The effect remained regardless of whether participants showed conscious aware- ness of activation. Theoretical and clinical implications are discussed.
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Differential effects on the Laser Evoked Potential of Selectively Attending to Pain Localisation versus Pain Unpleasantness.
Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology, 2004Co-Authors: De Bentley, Anthony W. Watson, Rolf-detlef Treede, G. Barrett, P Youell, B Kulkarni, Anthony K. P. JonesAbstract:Abstract Objective : To determine the effects on the Laser Evoked Potential (LEP) of selectively attending to affective (unpleasantness) versus sensory-discriminative (localisation) components of pain. Methods : LEPs, elicited by painful CO 2 Laser stimulation of two areas of the right forearm, were recorded from 62 electrodes in 21 healthy volunteers, during three tasks that were matched for generalised attention: Localisation (report stimulus location), Unpleasantness (report stimulus unpleasantness), Control (report pain detection). LEP components are named by polarity, latency, and electrode. Results : N300-T7 peak amplitude was significantly greater during Localisation than Unpleasantness. The difference in N300-T7 amplitude between Localisation and Control approached significance, suggesting an increased amplitude in Localisation compared with Control, rather than a reduced amplitude in Unpleasantness. Peak amplitude, latency, and topography of N300-FCz, P450, P600–800 (early P3) and P800–1000 (late P3) did not differ significantly between tasks. Conclusions : These results suggest that the N300-T7 LEP peak reflects the activity of cerebral generators involved in the localisation of pain. The topography of N300-T7 is consistent with a source in contralateral secondary somatosensory cortex/insula and maybe primary somatosensory cortex. Significance : This study confirms a role of the lateral pain system in the localisation of pain, and distinguishes it from stimulus novelty or attention.
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caudal cingulate cortex involvement in pain processing an inter individual Laser Evoked Potential source localisation study using realistic head models
Pain, 2003Co-Authors: De Bentley, P Youell, Stuart W G Derbyshire, Anthony K. P. JonesAbstract:Electrophysiological studies have revealed a source of Laser pain Evoked Potentials (LEPs) in cingulate cortex. However, few studies have used realistically shaped head models in the source analysis, which account for individual differences in anatomy and allow detailed anatomical localisation of sources. The aim of the current study was to accurately localise the cingulate source of LEPs in a group of healthy volunteers, using realistic head models, and to assess the inter-individual variability in anatomical location. LEPs, elicited by painful CO(2) Laser stimulation of the right forearm, were recorded from 62 electrodes in five healthy subjects. Dipole source localisation (CURRY 4.0) was performed on the most prominent (P2) peak of each LEP data set, using head models derived from each subject's structural magnetic resonance image (MRI).For all subjects, the P2 LEP peak was best explained by a dipole whose origin was in cingulate cortex (mean residual variance was 3.9+/-2.4 %). For four out of five subjects, it was located at the border of the caudal division of left anterior cingulate cortex (area 24/32') with left posterior cingulate cortex (area 23/31). For the fifth subject the dipole was centred in right posterior cingulate cortex (area 31). This study demonstrates that the location of the cingulate source of LEPs is highly consistent across subjects, when analysed in this way, and supports the involvement of caudal cingulate regions in pain processing.
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anatomical localization and intra subject reproducibility of Laser Evoked Potential source in cingulate cortex using a realistic head model
Clinical Neurophysiology, 2002Co-Authors: De Bentley, P Youell, Anthony K. P. JonesAbstract:Objectives: To (i) accurately localize the cingulate source of late Laser Evoked Potentials (LEPs) using a realistic head model incorporating the individual's anatomy and (ii) assess the within-subject reproducibility of this source. Methods: Late LEPs, elicited by painful CO2 Laser stimulation of the right forearm, were recorded from 62 electrodes in one healthy subject. This was repeated 9 times, over 3 different days. Dipole source localization (CURRY 4.0) was performed on the most prominent (P2) peak of each LEP data set, using a head model derived from the subject's structural magnetic resonance image. Results: In all cases the P2 LEP peak was best explained by a dipole located close to the border of the caudal division of left anterior cingulate cortex with left posterior cingulate cortex (mean residual variance was 1.7±0.4%). The maximum standard deviation from the mean dipole location was 3.2 mm. Conclusions: This study demonstrates that the location of the cingulate source of late LEPs is highly reproducible within this subject, when analyzed in this way, and suggests involvement of caudal cingulate regions in pain processing.
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Homotopic reduction in Laser-Evoked Potential amplitude and Laser-pain rating by abdominal acupuncture.
European journal of pain (London England), 2020Co-Authors: Massimiliano Valeriani, Catello Vollono, Stefano Liguori, E. Testani, Sergio Bangrazi, Filomena Petti, Aldo Liguori, Marco Germanotta, Luca Padua, Costanza PazzagliaAbstract:BACKGROUND The neural mechanism underlying the analgesic effect of acupuncture is largely unknown. We aimed at investigating the effect of abdominal acupuncture (AA) on the Laser-Evoked Potential (LEP) amplitude and Laser-pain rating to stimulation of body parts either homotopic or heterotopic to the treated acupoint. METHODS Laser-Evoked Potentials were recorded from 13 healthy subjects to stimulation of the right wrist (RW), left wrist (LW) and right foot (RF). LEPs were obtained before, during and after the AA stimulation of an abdominal area corresponding to the representation of the RW. Subjective Laser-pain rating was collected after each LEP recording. RESULTS The amplitude of the N2/P2 LEP component was significantly reduced during AA and 15 min after needle removal to both RW (F = 4.14, p = .02) and LW (F = 5.48, p = .008) stimulation, while the N2/P2 amplitude to RF stimulation (F = 0.94, p = .4) remained unchanged. Laser-pain rating was reduced during AA and 15 min after needle removal only to RW stimulation (F = 5.67, p = .007). CONCLUSION Our findings showing an AA effect on LEP components to both the ipsilateral and contralateral region homotopic to the treated area, without any LEP change to stimulation of a heterotopic region, suggest that the AA analgesia is mediated by a segmental spinal mechanism. SIGNIFICANCE Although abdominal acupuncture has demonstrated to be effective in the reduction in Laser-Evoked Potential (LEP) amplitude and Laser-pain rating, the exact mechanism of this analgesic effect is not known. In the current study, we found that treatment of an area in the "turtle representation" of the body led to a topographical pattern of LEP amplitude inhibition that can be mediated by a segmental spinal mechanism.
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znrd1 as and rp11 819c21 1 long non coding rna changes following painful Laser stimulation correlate with Laser Evoked Potential amplitude and habituation in healthy subjects a pilot study
European Journal of Pain, 2020Co-Authors: Massimo Santoro, Costanza Pazzaglia, Catello Vollono, Luca Padua, Rocco Giordano, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:BACKGROUND Non-coding RNAs (lncRNAs) are a group of non-coding RNAs that act as regulators of gene expression; they are implicated in various human diseases and have been reported to be involved in the modulation of pain. We aimed to study whether: (a) lncRNAs modifications could be found in an experimental model of pain; (b) there was a correlation between lncRNA changes and Laser Evoked Potential (LEP) amplitude/Laser-pain rating. METHODS Laser Evoked Potentials were recorded from 11 healthy subjects to both left hand and perioral region stimulation. Three consecutive averages were calculated for each stimulation site in order to investigate the LEP amplitude habituation. Blood samples were obtained immediately before LEP recording (pre-pain) and 30-min after the recording of the last LEP average (post-pain). Eighty-four lncRNAs, involved in autoimmunity and human inflammatory response, were screened. The criteria used for lncRNAs analysis were fold change >2 and p < .05. By Real-Time PCR, we identified two lncRNAs up-regulated at the post-pain time, as compared to the pre-pain time: RP11-819C21.1 (fold change = 8.2; p = .038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS; fold change = 6.3; p = .037). RESULTS The ZNRD1-AS up-regulation was directly correlated with the N1 amplitude, while the RP11-819C21.1 increase after pain showed a correlation with the reduced N2/P2 amplitude and Laser-pain habituation. CONCLUSION IncRNA changes in a human experimental phasic pain model. The correlation between lncRNA changes and LEP amplitude and habituation suggests that RP11-819C21.1 and ZNRD1-AS could be involved in the pathophysiology of painful diseases characterized by abnormal excitability of the cerebral cortex. SIGNIFICANCE Long non-coding RNAs are upregulated after experimental pain. RP11-819C21.1 and ZNRD1 could be involved in the pathophysiology of diseases characterized by reduced habituation to pain.
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p46 s rp11 819c21 1 and znrd1 as long non coding rna changes following experimental pain correlate with Laser Evoked Potential habituation
Clinical Neurophysiology, 2019Co-Authors: Catello Vollono, Costanza Pazzaglia, Luca Padua, Rocco Giordano, Massimo Santoro, Enrica Di Sipio, Lars Arendtnielsen, Massimiliano ValerianiAbstract:Background Long non-coding RNAs (lncRNAs) are a group of non-coding RNAs acting as regulators of gene expression, through interaction with histones or through interaction with complementary DNA sequences, implicated in various human diseases such as cancer, cardiovascular diseases, autoimmune and neurodegenerative disorders and have been reported to be involved in the modulation of neuropathic pain. We recorded Laser Evoked Potentials (LEPs) in order to study: (1) lncRNAs modifications in experimental pain model; (2) correlation between the lncRNA changes and objective measure of pain perception. Material and methods LEPs were recorded in 11 healthy subjects after hand and perioral region stimulation. Three consecutive series were recorded for each stimulation site in order to investigate the habituation. Blood samples were collected immediately before LEP recording (baseline) and after 30-min (post-pain). We screened 84 lncRNAs, involved in autoimmunity and inflammatory response. Results We identified 2 lncRNAs up-regulated at the post-pain time: RP11-819C21.1 (fold change = 8.2; p = 0.038) and ZNRD1 antisense RNA 1 non-protein coding (ZNRD1-AS) (fold change = 6.3; p = 0.037). The up-regulation of both lncRNAs showed a significant positive correlation with the LEP habituation to perioral region stimulation (p = 0.04 and p = 0.01, respectively). Conclusions This is the first study showing lncRNA changes in a pain experimental model. RP11-819C21.1 and ZNRD1-AS shows as direct target miR-19a and miR19b, a class of microRNAs involved in modulation of multiple potassium channel α-subunits. lncRNAs could be involved in the pathophysiology of painful diseases characterized by reduced habituation to pain.
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Expectation to feel more pain disrupts the habituation of Laser-pain rating and Laser-Evoked Potential amplitudes.
Neuroscience, 2016Co-Authors: Costanza Pazzaglia, Luca Padua, Elisa Testani, Rocco Giordano, Massimiliano ValerianiAbstract:Increased pain perception due to the expectation to feel more pain is called nocebo effect. The present study aimed at investigating whether: (1) the mere expectation to feel more pain after the administration of an inert drug can affect the Laser-pain rating and the Laser-Evoked Potential (LEP) amplitude, and (2) the learning potentiates the nocebo effect. Eighteen healthy volunteers were told that an inert cream, applied on the right hand, would increase the Laser pain and LEP amplitude to right hand stimulation. They were randomly assigned to either "verbal session" or "conditioning session". In the "verbal session", LEPs to both right and left hand stimulation were recorded at the same intensity before (baseline) and after cream application. In the "conditioning session", after an initial cream application the Laser stimulus intensity was increased surreptitiously to make the subjects believe that the treatment really increased the pain sensation. Then, the cream was reapplied, and LEPs were recorded at the same stimulus intensity as at the baseline. It was found that the verbal suggestion to feel more pain disrupted the physiological habituation of the Laser-pain rating and LEP amplitude to treated (right) hand stimulation. Unlike previously demonstrated for the placebo effect, the learning did not potentiate the nocebo effect.
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abdominal acupuncture reduces Laser Evoked Potentials in healthy subjects
Clinical Neurophysiology, 2015Co-Authors: Costanza Pazzaglia, Stefano Liguori, Aldo Liguori, Luca Padua, Elisa Testani, Ileana Minciotti, Ae Tozzi, F Petti, Massimiliano ValerianiAbstract:Abstract Objective Acupuncture is known to reduce clinical pain, although the exact mechanism is unknown. The aim of the current study was to investigate the effect of acupuncture on Laser-Evoked Potential amplitudes and Laser pain perception. Methods In order to evaluate whether abdominal acupuncture is able to modify pain perception, 10 healthy subjects underwent a protocol in which Laser-Evoked Potentials (LEPs) and Laser pain perception were collected before the test (baseline), during abdominal acupuncture, and 15min after needle removal. The same subjects also underwent a similar protocol in which, however, sham acupuncture without any needle penetration was used. Results During real acupuncture, both N1 and N2/P2 amplitudes were reduced, as compared to baseline ( p 0.01). The reduction lasted up to 15min after needle removal. Furthermore, Laser pain perception was reduced during real acupuncture, although the difference was marginally significant ( p =0.06). Conclusions Our results show that abdominal acupuncture reduces LEP amplitude in healthy subjects. Significance Our results provide a theoretical background for the use of abdominal acupuncture as a therapeutic approach in the treatment of pain conditions. Future studies will have to be conducted in clinical painful syndromes, in order to confirm the analgesic effect of acupuncture in patients suffering from pain.
