The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Zhongzhen Zhao - One of the best experts on this subject based on the ideXlab platform.
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tissue specific chemical profiling and quantitative analysis of bioactive components of cinnamomum cassia by combining Laser Microdissection with uplc q tof ms
Chemistry Central Journal, 2018Co-Authors: Zhitao Liang, Zhongzhen Zhao, Wenwen Zhou, Jun ChenAbstract:Cinnamomi Cortex, the dried stem bark of Cinnamomum cassia Presl (Rougui in Chinese) has been widely used in traditional Chinese medicine, cooking and perfumery for thousands of years. Traditionally, the Cinnamomi Cortex of thick size is considered to be of good quality; however, there is no scientific data to support this point. Considering that essential oils are the main bioactive components, Cinnamomi Cortex of greater variety and amount essential oils is thought to be of better quality. In this study, Laser Microdissection coupled with ultra-high performance liquid chromatography-quadrupole/time-of-flight-mass spectrometry (UPLC-Q/TOF–MS) was applied to profile the essential oils in different tissues of Cinnamomi Cortex and to determine if there is a correlation between the essential oil content and the stem bark thickness. We report the tissue-specific metabolic profiles of different grades of Cinnamomi Cortex. Nineteen chemical components were unequivocally or tentatively identified in the chromatogram of the test samples. The results indicate that the bioactive components, the essential oils, were mainly present in the phloem. Phloem thickness is the key character for evaluating the quality of Cinnamomi Cortex. Our results can be of great importance in improving the cultivation, harvesting, and processing of Cinnamomi Cortex, as well as enhancing its effects in clinical applications.
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Laser Microdissection hyphenated with high performance gel permeation chromatography charged aerosol detector and ultra performance liquid chromatography triple quadrupole mass spectrometry for histochemical analysis of polysaccharides in herbal medi
International Journal of Biological Macromolecules, 2018Co-Authors: Qilei Chen, Hubiao Chen, Yujie Chen, Shanshan Zhou, Kaman Yip, Zhongzhen ZhaoAbstract:Abstract This study establishes a new combinatorial approach for histochemical analysis of polysaccharides in herbal medicines using Laser Microdissection followed by high performance gel permeation chromatography coupled with charged aerosol detector and ultra-performance liquid chromatography hyphenated with triple quadrupole mass spectrometry. Ginseng was employed as a study model. Tissue-specific qualitative and quantitative characterization of ginseng polysaccharides was performed by determining their molar masses and monosaccharide compositions in three macro-dissected parts (rhizome, main and branched roots) and five micro-dissected tissues (cork, cortex, xylem, phloem and resin canal). The results showed that ginseng “flesh” (xylem, phloem and resin canal) contained more polysaccharides with larger molecular weights and higher ratios of glucose residue, whereas ginseng “skin” (cork and cortex) had fewer polysaccharides with smaller molecular weights and higher ratios of non-glucose constituents ( e.g. galacturonic acid, galactose, arabinose and rhamnose). These findings suggested that the polysaccharides of the “flesh” were predominantly starch-like glucans, while those of the “skin” were of a higher proportion of acidic pectins. The revealed histologic distribution and accumulation pattern of ginseng polysaccharides contributes to the scientific understanding of ginseng regarding the biosynthesis and transportation of polysaccharides, medicinal quality evaluation as well as empirical clinical application.
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histochemical evaluation of alkaloids in rhizome of coptis chinensis using Laser Microdissection and liquid chromatography mass spectrometry
Drug Testing and Analysis, 2015Co-Authors: Zhitao Liang, Zhongzhen Zhao, Ling Yi, Yong Peng, Lailai Wong, Hingman Ho, Tao YiAbstract:Traditional macroscopic and microscopic identification methods of medicinal materials are economical and practical, but usually experience-based due to few chemical supports. Here histochemical evaluation on bioactive components of Coptidis Rhizoma (CR) in anatomic sections using Laser Microdissection and liquid chromatography-mass spectrometry (LMD-LC-MS) was developed to correlate the inner quality and outer features of materials from different growing areas. Results of a total 33 peaks representing potential different alkaloids were detected and 8 common peaks were identified as the major alkaloids, namely magnoflorine, thalifendine, columbamine, epiberberine, jatrorrhizine, coptisine, palmatine, and berberine. Six major alkaloids were quantified in the top and middle sections of raw materials and in their tissues and cells at the same time. Histochemical analyses showed consistent results with direct determination in raw materials and explained the reason why top sections of all samples contained higher contents of alkaloids by giving out attributions of each alkaloid in different anatomic sections. Besides, results manifested the distribution and accumulation rules of alkaloids in diverse tissues and cells of CR. This study demonstrates an effective and scientific way to correlate bioactive components and morphological features of medicinal materials, which is beneficial to future research, agriculture and application. Copyright © 2014 John Wiley & Sons, Ltd.
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whole transverse section and specific tissue analysis of secondary metabolites in seven different grades of root of paeonia lactiflora using Laser Microdissection and liquid chromatography quadrupole time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2015Co-Authors: Qiuling Wang, Zhitao Liang, Zhongzhen Zhao, Yong Peng, Wen Quan WangAbstract:Abstract The root of Paeonia lactiflora Pall. is widely used in the pharmaceutical, food and cosmetic industries. For these purposes, roots are graded according to diameter, with larger roots considered to be of better quality. To assess the inherent quality of different grades and of different tissues in roots of P. lactiflora , here Laser Microdissection coupled with UPLC-Q/TOF–MS was applied. The results show the quantity of pharmaceutically important components decreased with increase in root diameter from 0.3 cm to 0.7 cm. Above 0.7 cm of diameter, quantity and diversity of these components increased proportionally with increase in root diameter. The tissue-specific study indicated that the high content of paeoniflorin and albiflorin are mainly distributed in the cork and cortex. According to the results of this study, the roots of P . lactiflora greater than 1.7 cm in diameter are of better quality medicinal use than smaller, and the commercial grades chose was best cutoff points. The fine roots and the outer bank of roots, which besides the commercial grades, contain such significant amounts of chemical components too. This study provides a new and practical method for evaluating the different grades of P. lactiflora .
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A comparative tissue-specific metabolite analysis and determination of protodioscin content in Asparagus species used in traditional Chinese medicine and Ayurveda by use of Laser Microdissection, UHPLC-QTOF/MS and LC-MS/MS.
Phytochemical Analysis, 2014Co-Authors: Yogini Jaiswal, Zhitao Liang, Hubiao Chen, Zhongzhen ZhaoAbstract:Introduction Asparagus is esteemed in Traditional Chinese Medicine and Ayurveda, and it is commercially one of the most important drugs in the global herbal market. Comparative metabolite profiling of different species would help in determining the similarities and ascertain their validity for being used as substitutes for each other. Laser Microdissection (LMD) facilitates identification of metabolites in specific tissues, and thus it can aid in exploration of metabolic pathways in target tissues. Objective To compare tissue-specific metabolites and protodioscin content of Asparagus cochinchinensis (Lour.) Merr. and Asparagus racemosus Willd. used in China and India. Methods Metabolite analysis of Laser-dissected tissues was carried out using UHPLC–QTOF/MS and LC–MS/MS. The protodioscin contents were determined and the method was validated as per the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. Results Metabolite analysis reveals that the velamen tissue, among other tissues such as cortex, vascular bundles and pith, contained maximum components, specifically those belonging to the steroidal saponin class. Although the metabolite profiles were similar, the content of protodioscin was found to be higher in Chinese than Indian species. Conclusion The study provided a suitable methodology for metabolite profiling and protodioscin content determination of Asparagus by use of LMD, UHPLC–QTOF/MS and LC–MS/MS. The similarities in metabolite profiles indicate that Asparagus species from India and China can serve as substitute for each other in various therapeutic and pharmaceutical applications. Copyright © 2014 John Wiley & Sons, Ltd.
Claudio Orlando - One of the best experts on this subject based on the ideXlab platform.
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isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer correlation with real time reverse transcriptase polymerase chain reaction results and feasibility of molecular analysis by Laser Microdissection
Human Pathology, 2006Co-Authors: Pamela Pinzani, Lisa Simi, Simonetta Ianchi, V Distante, L Cataliotti, Mario Pazzagli, Claudio OrlandoAbstract:Summary The aim of this study is the counting and the immunomorphological and molecular characterization of circulating tumor cells (CTCs) by the isolation by size of epithelial tumor cells (ISET) method in the peripheral blood of patients with breast cancer. An evaluation of the method's ability to reveal the presence of occult carcinoma cells in blood of a patient with breast cancer was performed and the results compared with those obtained by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for the evaluation of cytokeratin-19 (CK-19) mRNA expression. The feasibility of molecular analysis of CTCs after Laser Microdissection of filters used in ISET was illustrated, referring to HER-2 amplification. Blood samples drawn from 44 patients with breast cancer were preoperatively analyzed by ISET. From the same samples, total RNA was extracted and submitted to quantitative real-time RT-PCR for the detection of CK-19 mRNA-positive cells using TaqMan technology. HER-2 amplification was measured by real-time RT-PCR on DNA extracted from cells recovered by Laser Microdissection from 7 selected ISET-positive filters. Of 44 samples, 12 (27%) showed the presence of epithelial cells on the filter (mean ± SE: 8.5 ± 2.4 cells per milliliter of blood). A statistically significant agreement ( P = .001) was observed between real-time RT-PCR results and those obtained by ISET. With regard to HER-2 amplification, a good correspondence was found between the results obtained from microdissected CTCs and those obtained using DNA extracted from the primary tumor ( R = 0.918; P
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isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer correlation with real time reverse transcriptase polymerase chain reaction results and feasibility of molecular analysis by Laser Microdissection
Human Pathology, 2006Co-Authors: Pamela Pinzani, Lisa Simi, V Distante, L Cataliotti, Mario Pazzagli, B Salvadori, Simonetta Bianchi, Claudio OrlandoAbstract:Summary The aim of this study is the counting and the immunomorphological and molecular characterization of circulating tumor cells (CTCs) by the isolation by size of epithelial tumor cells (ISET) method in the peripheral blood of patients with breast cancer. An evaluation of the method's ability to reveal the presence of occult carcinoma cells in blood of a patient with breast cancer was performed and the results compared with those obtained by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for the evaluation of cytokeratin-19 (CK-19) mRNA expression. The feasibility of molecular analysis of CTCs after Laser Microdissection of filters used in ISET was illustrated, referring to HER-2 amplification. Blood samples drawn from 44 patients with breast cancer were preoperatively analyzed by ISET. From the same samples, total RNA was extracted and submitted to quantitative real-time RT-PCR for the detection of CK-19 mRNA-positive cells using TaqMan technology. HER-2 amplification was measured by real-time RT-PCR on DNA extracted from cells recovered by Laser Microdissection from 7 selected ISET-positive filters. Of 44 samples, 12 (27%) showed the presence of epithelial cells on the filter (mean ± SE: 8.5 ± 2.4 cells per milliliter of blood). A statistically significant agreement ( P = .001) was observed between real-time RT-PCR results and those obtained by ISET. With regard to HER-2 amplification, a good correspondence was found between the results obtained from microdissected CTCs and those obtained using DNA extracted from the primary tumor ( R = 0.918; P
Hiroyuki Sakashita - One of the best experts on this subject based on the ideXlab platform.
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identification of the nedd4l gene as a prognostic marker by integrated microarray analysis of copy number and gene expression profiling in non small cell lung cancer
Annals of Surgical Oncology, 2013Co-Authors: Hiroyuki Sakashita, Hiroshi Inoue, Shinji Akamine, Teruyoshi Ishida, Naohiko Inase, Kuniaki Shirao, Masaki MoriAbstract:Purpose The purpose of this study was to identify prognostic genes by integrated microarray analysis between comparative genomic hybridization and gene expression with Laser Microdissection in non-small cell lung cancer (NSCLC).
Masaki Mori - One of the best experts on this subject based on the ideXlab platform.
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identification of the nedd4l gene as a prognostic marker by integrated microarray analysis of copy number and gene expression profiling in non small cell lung cancer
Annals of Surgical Oncology, 2013Co-Authors: Hiroyuki Sakashita, Hiroshi Inoue, Shinji Akamine, Teruyoshi Ishida, Naohiko Inase, Kuniaki Shirao, Masaki MoriAbstract:Purpose The purpose of this study was to identify prognostic genes by integrated microarray analysis between comparative genomic hybridization and gene expression with Laser Microdissection in non-small cell lung cancer (NSCLC).
Zhitao Liang - One of the best experts on this subject based on the ideXlab platform.
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tissue specific chemical profiling and quantitative analysis of bioactive components of cinnamomum cassia by combining Laser Microdissection with uplc q tof ms
Chemistry Central Journal, 2018Co-Authors: Zhitao Liang, Zhongzhen Zhao, Wenwen Zhou, Jun ChenAbstract:Cinnamomi Cortex, the dried stem bark of Cinnamomum cassia Presl (Rougui in Chinese) has been widely used in traditional Chinese medicine, cooking and perfumery for thousands of years. Traditionally, the Cinnamomi Cortex of thick size is considered to be of good quality; however, there is no scientific data to support this point. Considering that essential oils are the main bioactive components, Cinnamomi Cortex of greater variety and amount essential oils is thought to be of better quality. In this study, Laser Microdissection coupled with ultra-high performance liquid chromatography-quadrupole/time-of-flight-mass spectrometry (UPLC-Q/TOF–MS) was applied to profile the essential oils in different tissues of Cinnamomi Cortex and to determine if there is a correlation between the essential oil content and the stem bark thickness. We report the tissue-specific metabolic profiles of different grades of Cinnamomi Cortex. Nineteen chemical components were unequivocally or tentatively identified in the chromatogram of the test samples. The results indicate that the bioactive components, the essential oils, were mainly present in the phloem. Phloem thickness is the key character for evaluating the quality of Cinnamomi Cortex. Our results can be of great importance in improving the cultivation, harvesting, and processing of Cinnamomi Cortex, as well as enhancing its effects in clinical applications.
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histochemical evaluation of alkaloids in rhizome of coptis chinensis using Laser Microdissection and liquid chromatography mass spectrometry
Drug Testing and Analysis, 2015Co-Authors: Zhitao Liang, Zhongzhen Zhao, Ling Yi, Yong Peng, Lailai Wong, Hingman Ho, Tao YiAbstract:Traditional macroscopic and microscopic identification methods of medicinal materials are economical and practical, but usually experience-based due to few chemical supports. Here histochemical evaluation on bioactive components of Coptidis Rhizoma (CR) in anatomic sections using Laser Microdissection and liquid chromatography-mass spectrometry (LMD-LC-MS) was developed to correlate the inner quality and outer features of materials from different growing areas. Results of a total 33 peaks representing potential different alkaloids were detected and 8 common peaks were identified as the major alkaloids, namely magnoflorine, thalifendine, columbamine, epiberberine, jatrorrhizine, coptisine, palmatine, and berberine. Six major alkaloids were quantified in the top and middle sections of raw materials and in their tissues and cells at the same time. Histochemical analyses showed consistent results with direct determination in raw materials and explained the reason why top sections of all samples contained higher contents of alkaloids by giving out attributions of each alkaloid in different anatomic sections. Besides, results manifested the distribution and accumulation rules of alkaloids in diverse tissues and cells of CR. This study demonstrates an effective and scientific way to correlate bioactive components and morphological features of medicinal materials, which is beneficial to future research, agriculture and application. Copyright © 2014 John Wiley & Sons, Ltd.
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whole transverse section and specific tissue analysis of secondary metabolites in seven different grades of root of paeonia lactiflora using Laser Microdissection and liquid chromatography quadrupole time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2015Co-Authors: Qiuling Wang, Zhitao Liang, Zhongzhen Zhao, Yong Peng, Wen Quan WangAbstract:Abstract The root of Paeonia lactiflora Pall. is widely used in the pharmaceutical, food and cosmetic industries. For these purposes, roots are graded according to diameter, with larger roots considered to be of better quality. To assess the inherent quality of different grades and of different tissues in roots of P. lactiflora , here Laser Microdissection coupled with UPLC-Q/TOF–MS was applied. The results show the quantity of pharmaceutically important components decreased with increase in root diameter from 0.3 cm to 0.7 cm. Above 0.7 cm of diameter, quantity and diversity of these components increased proportionally with increase in root diameter. The tissue-specific study indicated that the high content of paeoniflorin and albiflorin are mainly distributed in the cork and cortex. According to the results of this study, the roots of P . lactiflora greater than 1.7 cm in diameter are of better quality medicinal use than smaller, and the commercial grades chose was best cutoff points. The fine roots and the outer bank of roots, which besides the commercial grades, contain such significant amounts of chemical components too. This study provides a new and practical method for evaluating the different grades of P. lactiflora .
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A comparative tissue-specific metabolite analysis and determination of protodioscin content in Asparagus species used in traditional Chinese medicine and Ayurveda by use of Laser Microdissection, UHPLC-QTOF/MS and LC-MS/MS.
Phytochemical Analysis, 2014Co-Authors: Yogini Jaiswal, Zhitao Liang, Hubiao Chen, Zhongzhen ZhaoAbstract:Introduction Asparagus is esteemed in Traditional Chinese Medicine and Ayurveda, and it is commercially one of the most important drugs in the global herbal market. Comparative metabolite profiling of different species would help in determining the similarities and ascertain their validity for being used as substitutes for each other. Laser Microdissection (LMD) facilitates identification of metabolites in specific tissues, and thus it can aid in exploration of metabolic pathways in target tissues. Objective To compare tissue-specific metabolites and protodioscin content of Asparagus cochinchinensis (Lour.) Merr. and Asparagus racemosus Willd. used in China and India. Methods Metabolite analysis of Laser-dissected tissues was carried out using UHPLC–QTOF/MS and LC–MS/MS. The protodioscin contents were determined and the method was validated as per the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. Results Metabolite analysis reveals that the velamen tissue, among other tissues such as cortex, vascular bundles and pith, contained maximum components, specifically those belonging to the steroidal saponin class. Although the metabolite profiles were similar, the content of protodioscin was found to be higher in Chinese than Indian species. Conclusion The study provided a suitable methodology for metabolite profiling and protodioscin content determination of Asparagus by use of LMD, UHPLC–QTOF/MS and LC–MS/MS. The similarities in metabolite profiles indicate that Asparagus species from India and China can serve as substitute for each other in various therapeutic and pharmaceutical applications. Copyright © 2014 John Wiley & Sons, Ltd.
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profiling of secondary metabolites in tissues from rheum palmatum l using Laser Microdissection and liquid chromatography mass spectrometry
Analytical and Bioanalytical Chemistry, 2013Co-Authors: Zhitao Liang, Hubiao Chen, Tungting Sham, Guangyi Yang, Zhongzhen ZhaoAbstract:Evaluating the quality of herbal medicines by morphological features is a convenient, quick, and practical method compared with other methods that mostly depend on modern instruments. Here, Laser Microdissection and ultra-performance liquid chromatography are combined with mass spectrometry to map the distribution of secondary metabolites in cells or tissues of a herb itself for correlating its bioactive components and morphological features. The root and rhizome of Rheum palmatum L. were taken as research target, which is the Chinese medicine, Radix et Rhizoma Rhei. According to fluorescent microscopic characteristics, 12 herbal cells or tissues of Radix et Rhizoma Rhei were separated by Laser Microdissection. Thirty-eight compounds were identified or tentatively characterized in the microdissected tissues. (+)-Catechin, 1-O-galloyl-2-O-cinnamoyl-β-d-glucose, and emodin were found to be the major components in most of the tissues. The brown ergastic substances found in rays of normal and anomalous vascular bundles as well as the parenchymatous cells of rhizome pith and the parenchymatous cells of root xylem contained higher than average amounts of these three components and more kinds of secondary metabolites. Overall, results suggest that Radix et Rhizoma Rhei of larger size and with conspicuous “brocaded patterns” and star spots are of higher quality as they tend to have greater contents of bioactive components. The study provides quantitative and specific criteria by which the quality of Radix et Rhizoma Rhei can be judged. This research also established a new, reliable, and practical method for direct profiling and imaging of secondary metabolites in any herbal tissue.
