The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Yanhui Chen - One of the best experts on this subject based on the ideXlab platform.
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ZmCLA4 regulates Leaf Angle through multiple plant hormone-mediated signal pathways in maize
2020Co-Authors: Yanhui Chen, Dandan Dou, Shengbo Han, Huafeng Liu, Dongling Zhang, Haixia Zeng, Yahui Dong, Zhixue LiuAbstract:Abstract Leaf Angle in cereals is an important agronomic trait contributing to plant architecture and grain yield by determining the plant compactness. Although ZmCLA4 was identified to shape plant architecture by affecting Leaf Angle, the detailed regulatory mechanism of ZmCLA4 in maize remains unclear. ZmCLA4 was identified as a transcriptional repressor using the Gal4-LexA/UAS system and transactivation analysis in yeast. The DNA affinity purification (DAP)-seq assay showed that ZmCLA4 not only acts as a repressor containing the EAR motif (CACCGGAC), but was also found to have two new motifs, CCGARGS and CDTCNTC. On analyzing the ZmCLA4-bound targeted genes, we found that ZmCLA4, as a cross node of multiple plant hormone-mediated pathways, directly bound to ARF22 and IAA26 to regulate auxin transport and mediated brassinosteroid signaling by directly binding to BZR3 and 14-3-3. ZmCLA4 bound two WRKY genes involved with abscisic acid, two genes (CYP75B1, CYP93D1) involved with jasmonic acid, B3 involved in the response to ethylene, and thereby negatively regulated Leaf Angle formation. We built a new regulatory network for the ZmCLA4 gene controlling Leaf Angle in maize, which contributed to the understanding of ZmCLA4’s regulatory mechanism and will improve grain yields by facilitating optimization of plant architecture.
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Differences in properties and proteomes of the midribs contribute to the size of the Leaf Angle in two near-isogenic maize lines.
Journal of proteomics, 2015Co-Authors: Ning Wang, Yanhui Chen, Di Cao, Fangping Gong, Wei WangAbstract:The midrib of maize leaves provides the primary support for the blade and is largely associated with Leaf Angle size. To elucidate the role of the midrib in Leaf Angle formation, the maize line Shen137 (larger Leaf Angle) and a near isogenic line (NIL, smaller Leaf Angle) were used in the present study. The results of the analysis showed that both the puncture forces and proximal collenchyma number of the midribs of the first and second leaves above the ear were higher in NIL than in Shen137. Comparative proteomic analysis was performed to reveal protein profile differences in the midribs of the 5th, 10th and 19th newly expanded leaves between Shen137 and NIL. Quantitative analysis of 24 identified midrib proteins indicated that the maximum changes in abundance of 22 proteins between Shen137 and NIL appeared at the 10th Leaf stage, of which phosphoglycerate kinase, adenosine kinase, fructose-bisphosphate aldolase and adenylate kinase were implicated in glycometabolism. Thus, glycometabolism might be associated with Leaf Angle formation and the physical and mechanical properties of the midribs. These results provide insight into the mechanism underlying maize Leaf Angle formation.
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The ZmCLA4 gene in the qLA4-1 QTL controls Leaf Angle in maize (Zea mays L.)
Journal of experimental botany, 2014Co-Authors: Jun Zhang, Shulei Guo, Liru Cao, Huafeng Liu, Zanping Han, Zhiyong Zhang, Xinjian Cui, Yanhui ChenAbstract:Maize architecture is a major contributing factor to their high level of productivity. Maize varieties with an erect-Leaf-Angle (LA) phenotype, which increases light harvesting for photosynthesis and grain-filling, have elevated grain yields. Although a large body of information is available on the map positions of quantitative trait loci (QTL) for LA, little is known about the molecular mechanism of these QTL. In this study, the ZmCLA4 gene, which is responsible for the qLA4-1 QTL associated with LA, was identified and isolated by fine mapping and positional cloning. The ZmCLA4 gene is an orthologue of LAZY1 in rice and Arabidopsis. Sequence analysis revealed two SNPs and two indel sites in ZmCLA4 between the D132 and D132-NIL inbred maize lines. Association analysis showed that C/T/mutation667 and CA/indel965 were strongly associated with LA. Subcellular localization verified the functions of a predicted transmembrane domain and a nuclear localization signal in ZmCLA4. Transgenic maize plants with a down-regulated ZmCLA4 RNAi construct and transgenic rice plants over-expressing ZmCLA4 confirmed that the ZmCLA4 gene located in the qLA4 QTL regulated LA. The allelic variants of ZmCLA4 in the D132 and D132-NIL lines exhibited significant differences in Leaf Angle. ZmCLA4 transcript accumulation was higher in D132-NIL than in D132 during all the developmental stages and was negatively correlated with LA. The gravitropic response was increased and cell shape and number at the Leaf and stem junctions were altered in D132-NIL relative to D132. These findings suggest that ZmCLA4 plays a negative role in the control of maize LA through the alteration of mRNA accumulation, leading to altered shoot gravitropism and cell development. The cloning of the gene responsible for the qLA4-1 QTL provides information on the molecular mechanisms of LA in maize and an opportunity for the improvement of plant architecture with regard to LA through maize breeding.
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Cloning and Characterization of a Putative TAC1 Ortholog Associated with Leaf Angle in Maize (Zea mays L.)
2013Co-Authors: Xiaomin Wei, Jun Zhang, Shaofang Zhang, Shulei Guo, Yanhui ChenAbstract:Background: Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling Leaf Angle in maize is needed to provide insight into molecular mechanism of Leaf development and achieving ideal plant architecture to improve grain yield. Methodology/Principal Findings: The gene cloning was done by using comparative genomics, and then performing realtime polymerase chain reaction (RT-PCR) analysis to assay gene expression. The gene function was validated by sequence dissimilarity analysis and QTL mapping using a functional cleaved amplified polymorphism (CAP). Conclusions: The Leaf Angle is controlled by a major quantitative trait locus, ZmTAC1 (Zea mays L. Leaf Angle Control 1). ZmTAC1 has 4 exons encoding a protein with 263 amino acids, and its domains are the same as those of the rice OsTAC1 protein. ZmTAC1 was found to be located in the region of qLA2 by using the CAP marker and the F 2:3 families from the cross between Yu82 and Shen137. Real-time PCR analysis revealed ZmTAC1 expression was the highest in the Leaf-sheath pulvinus, less in the Leaf and shoot apical meristem, and the lowest in the root. A nucleotide difference in the 59untranslated region (UTR) between the compact inbred line Yu82 (‘‘CTCC’’) and the expanded inbred line Shen137 (‘‘CCCC’’) influences the expression level of ZmTAC1, further controlling the size of the Leaf Angle. Sequence verification of the chang
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cloning and characterization of a putative tac1 ortholog associated with Leaf Angle in maize zea mays l
PLOS ONE, 2011Co-Authors: Xiaomin Wei, Jun Zhang, Shaofang Zhang, Shulei Guo, Yanhui ChenAbstract:Background Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling Leaf Angle in maize is needed to provide insight into molecular mechanism of Leaf development and achieving ideal plant architecture to improve grain yield.
Qian Chen - One of the best experts on this subject based on the ideXlab platform.
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wheat taspl8 modulates Leaf Angle through auxin and brassinosteroid signaling
Plant Physiology, 2019Co-Authors: Kaiye Liu, Jie Cao, Xinye Liu, Yujiao Gao, Qian ChenAbstract:In grass crops, Leaf Angle is determined by development of the lamina joint, the tissue connecting the Leaf blade and sheath, and is closely related to crop architecture and yield. In this study, we identified a mutant generated by fast neutron radiation that exhibited an erect Leaf phenotype caused by defects in lamina joint development. Map-based cloning revealed that the gene TaSPL8, encoding a SQUAMOSA PROMOTER BINDING-LIKE (SPL) protein, is deleted in this mutant. TaSPL8 knock-out mutants exhibit erect leaves due to loss of the lamina joint, compact architecture, and increased spike number especially in high planting density, suggesting similarity with its LIGULESS1 homologs in maize (Zea mays) and rice (Oryza sativa). Hence, LG1 could be a robust target for plant architecture improvement in grass species. Common wheat (Triticum aestivum, 2n = 6× = 42; BBAADD) is an allohexaploid containing A, B, and D subgenomes and the homeologous gene of TaSPL8 from the D subgenome contributes to the length of the lamina joint to a greater extent than that from the A and B subgenomes. Comparison of the transcriptome between the Taspl8 mutant and the wild type revealed that TaSPL8 is involved in the activation of genes related to auxin and brassinosteroid pathways and cell elongation. TaSPL8 binds to the promoters of the AUXIN RESPONSE FACTOR gene and of the brassinosteroid biogenesis gene CYP90D2 and activates their expression. These results indicate that TaSPL8 might regulate lamina joint development through auxin signaling and the brassinosteroid biosynthesis pathway.
K. C. Woo - One of the best experts on this subject based on the ideXlab platform.
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Influence of Leaf Angle on photosynthesis and the xanthophyll cycle in the tropical tree species Acacia crassicarpa
Tree physiology, 2003Co-Authors: Li-xia Liu, K. C. WooAbstract:We examined the effects of artificially altering Leaf Angle of the tropical tree species Acacia crassicarpa (A. Cunn. ex Benth., Fabaceae) on light interception, Leaf temperature and photosynthesis in the wet and dry seasons of tropical Australia. Reducing Leaf Angle from the natural near-vertical Angle (90degrees) to 67.5degrees, 45degrees, 22.5degrees and 0degrees greatly increased light interception and Leaf temperature, and decreased photosynthetic activity. Compared with the 90degrees phyllodes, net photosynthetic rates in the horizontal phyllodes decreased by 18 and 42% by the second day of Leaf Angle change in the wet and dry seasons, respectively. The corresponding values for Day 7 were 46 and 66%. Leaf Angle reduction also altered the diurnal pattern of photosynthesis (from two peaks to one peak) and reduced daily CO2 fixation by 23-50% by Day 2 and by 50-75% by Day 7 in the dry season. In contrast, the xanthophyll cycle pool size in the phyllodes increased with Leaf Angle reduction. Thus, there are at least five major advantages to maintaining high Leaf Angle orientation in tropical tree species. First, it reduces excessive light interception. Second, it lowers Leaf temperature. Third, it protects the photosynthetic apparatus against photodamage by excessive light. Fourth, it minimizes xanthophyll cycle activity and reduces the cost for xanthophyll biosynthesis. Finally, it enhances photosynthetic activity and helps to sustain high plant productivity.
Christopher L. Beadle - One of the best experts on this subject based on the ideXlab platform.
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Leaf Angle responds to nitrogen supply in eucalypt seedlings. Is it a photoprotective mechanism
Tree Physiology, 2006Co-Authors: Dugald C. Close, Christopher L. BeadleAbstract:We examined the adjustment of Leaf Angle (L θ) and foliar chlorophyll and xanthophyll chemistry in Eucalyptus nitens (Deane and Maiden) Maiden seedlings maintained in various nitrogen (N)-supply treatments over a 6-month period. Adjustment of L θ toward the vertical was greatest under conditions of foliar N deficiency and became incrementally more horizontal with increasing foliar N concentration. Photochemical efficiency (F v/F m) and quantum yield were lower in seedlings with low foliar N (low-N seedlings) in winter, but not in autumn. Low-N seedlings generally had low area-based chlorophyll concentrations and high xanthophyll-cycle conversion ratios, particularly during months of low temperature. Under mild temperature conditions, high concentrations of zeaxanthin and antheraxanthin were associated with lower electron transport rates (ETR). Incident light, F v/F m, ETR and total chlorophyll concentration were negatively correlated with L θ, with horizontal Leaf orientation measured as 0° and vertical Leaf orientation as 90°. Xanthophyll conversion ratio was positively correlated with L θ. Adjustments in L θ may play a role in photoprotection of E. nitens seedlings by assisting the Leaf to balance its utilization and dissipation of energy. © 2006 Heron Publishing.
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Leaf Angle responds to nitrogen supply in eucalypt seedlings. Is it a photoprotective mechanism
Tree physiology, 2006Co-Authors: Dugald C. Close, Christopher L. BeadleAbstract:We examined the adjustment of Leaf Angle (L theta) and foliar chlorophyll and xanthophyll chemistry in Eucalyptus nitens (Deane and Maiden) Maiden seedlings maintained in various nitrogen (N)-supply treatments over a 6-month period. Adjustment of L theta toward the vertical was greatest under conditions of foliar N deficiency and became incrementally more horizontal with increasing foliar N concentration. Photochemical efficiency (Fv/Fm) and quantum yield were lower in seedlings with low foliar N (low-N seedlings) in winter, but not in autumn. Low-N seedlings generally had low area-based chlorophyll concentrations and high xanthophyll-cycle conversion ratios, particularly during months of low temperature. Under mild temperature conditions, high concentrations of zeaxanthin and antheraxanthin were associated with lower electron transport rates (ETR). Incident light, Fv/Fm, ETR and total chlorophyll concentration were negatively correlated with L theta, with horizontal Leaf orientation measured as 0 degrees and vertical Leaf orientation as 90 degrees . Xanthophyll conversion ratio was positively correlated with L theta. Adjustments in L theta may play a role in photoprotection of E. nitens seedlings by assisting the Leaf to balance its utilization and dissipation of energy.
Jun Zhang - One of the best experts on this subject based on the ideXlab platform.
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The ZmCLA4 gene in the qLA4-1 QTL controls Leaf Angle in maize (Zea mays L.)
Journal of experimental botany, 2014Co-Authors: Jun Zhang, Shulei Guo, Liru Cao, Huafeng Liu, Zanping Han, Zhiyong Zhang, Xinjian Cui, Yanhui ChenAbstract:Maize architecture is a major contributing factor to their high level of productivity. Maize varieties with an erect-Leaf-Angle (LA) phenotype, which increases light harvesting for photosynthesis and grain-filling, have elevated grain yields. Although a large body of information is available on the map positions of quantitative trait loci (QTL) for LA, little is known about the molecular mechanism of these QTL. In this study, the ZmCLA4 gene, which is responsible for the qLA4-1 QTL associated with LA, was identified and isolated by fine mapping and positional cloning. The ZmCLA4 gene is an orthologue of LAZY1 in rice and Arabidopsis. Sequence analysis revealed two SNPs and two indel sites in ZmCLA4 between the D132 and D132-NIL inbred maize lines. Association analysis showed that C/T/mutation667 and CA/indel965 were strongly associated with LA. Subcellular localization verified the functions of a predicted transmembrane domain and a nuclear localization signal in ZmCLA4. Transgenic maize plants with a down-regulated ZmCLA4 RNAi construct and transgenic rice plants over-expressing ZmCLA4 confirmed that the ZmCLA4 gene located in the qLA4 QTL regulated LA. The allelic variants of ZmCLA4 in the D132 and D132-NIL lines exhibited significant differences in Leaf Angle. ZmCLA4 transcript accumulation was higher in D132-NIL than in D132 during all the developmental stages and was negatively correlated with LA. The gravitropic response was increased and cell shape and number at the Leaf and stem junctions were altered in D132-NIL relative to D132. These findings suggest that ZmCLA4 plays a negative role in the control of maize LA through the alteration of mRNA accumulation, leading to altered shoot gravitropism and cell development. The cloning of the gene responsible for the qLA4-1 QTL provides information on the molecular mechanisms of LA in maize and an opportunity for the improvement of plant architecture with regard to LA through maize breeding.
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Cloning and Characterization of a Putative TAC1 Ortholog Associated with Leaf Angle in Maize (Zea mays L.)
2013Co-Authors: Xiaomin Wei, Jun Zhang, Shaofang Zhang, Shulei Guo, Yanhui ChenAbstract:Background: Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling Leaf Angle in maize is needed to provide insight into molecular mechanism of Leaf development and achieving ideal plant architecture to improve grain yield. Methodology/Principal Findings: The gene cloning was done by using comparative genomics, and then performing realtime polymerase chain reaction (RT-PCR) analysis to assay gene expression. The gene function was validated by sequence dissimilarity analysis and QTL mapping using a functional cleaved amplified polymorphism (CAP). Conclusions: The Leaf Angle is controlled by a major quantitative trait locus, ZmTAC1 (Zea mays L. Leaf Angle Control 1). ZmTAC1 has 4 exons encoding a protein with 263 amino acids, and its domains are the same as those of the rice OsTAC1 protein. ZmTAC1 was found to be located in the region of qLA2 by using the CAP marker and the F 2:3 families from the cross between Yu82 and Shen137. Real-time PCR analysis revealed ZmTAC1 expression was the highest in the Leaf-sheath pulvinus, less in the Leaf and shoot apical meristem, and the lowest in the root. A nucleotide difference in the 59untranslated region (UTR) between the compact inbred line Yu82 (‘‘CTCC’’) and the expanded inbred line Shen137 (‘‘CCCC’’) influences the expression level of ZmTAC1, further controlling the size of the Leaf Angle. Sequence verification of the chang
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cloning and characterization of a putative tac1 ortholog associated with Leaf Angle in maize zea mays l
PLOS ONE, 2011Co-Authors: Xiaomin Wei, Jun Zhang, Shaofang Zhang, Shulei Guo, Yanhui ChenAbstract:Background Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling Leaf Angle in maize is needed to provide insight into molecular mechanism of Leaf development and achieving ideal plant architecture to improve grain yield.
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quantitative trait loci mapping of Leaf Angle and Leaf orientation value in maize zea mays l
Theoretical and Applied Genetics, 2010Co-Authors: Lixia Ku, P A Wang, W Q Zhang, W.m. Zhao, Liuji Wu, Cuiling Wang, Jun Zhang, Yanhui ChenAbstract:A major limiting factor for high productivity of maize (Zea mays L.) in dense planting is light penetration through the canopy. Plant architecture with a narrower Leaf Angle (LA) and an optimum Leaf orientation value (LOV) is desirable to increase light capture for photosynthesis and production per unit area. However, the genetic control of the plant architecture traits remains poorly understood in maize. In this study, QTL for LA, LOV, and related traits were mapped using a set of 229 F2:3 families derived from the cross between compact and expanded inbred lines, evaluated in three environments. Twenty-five QTL were detected in total. Three of the QTL explained 37.4% and five of the QTL explained 53.9% of the phenotypic variance for LA and LOV, respectively. Two key genome regions controlling Leaf Angle and Leaf orientation were identified. qLA1 and qLOV1 at nearest marker umc2226 on chromosome 1.02 accounted for 20.4 and 23.2% of the phenotypic variance, respectively; qLA5 and qLOV5 at nearest bnlg1287 on chromosome 5 accounted for 9.7 and 9.8% of the phenotypic variance, respectively. These QTL could provide useful information for marker-assisted selection in improving performance of plant architecture with regard to Leaf Angle and orientation.