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J A Kolmer - One of the best experts on this subject based on the ideXlab platform.
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adult plant Leaf Rust resistance in ac taber wheat maps to chromosomes 2bs and 3bs
Phytopathology, 2021Co-Authors: J A Kolmer, Matthew N Rouse, M K Turner, James A AndersonAbstract:AC Taber is a hard red spring wheat cultivar that has had long-lasting resistance to the Leaf Rust fungus Puccinia triticina. The objective of this study was to determine the chromosome location of the Leaf Rust resistance genes in AC Taber. The Leaf Rust-susceptible cultivar Thatcher was crossed with AC Taber to develop an F6 recombinant inbred line (RIL) population. The RILs and parents were evaluated for segregation of Leaf Rust resistance in five field plot tests and in two seedling tests to race BBBDB of P. triticina. A genetic map of the RIL population was developed using 90,000 single nucleotide polymorphism markers with the Illumina Infinium iSelect 90K wheat bead array. Quantitative trait loci (QTLs) with significant effects for lower Leaf Rust severity in the field plot tests were found on chromosomes 2BS and 3BS. The same QTLs also had significant effects for lower infection type in seedlings to Leaf Rust race BBBDB. The gene on 2BS was the adult plant resistance gene Lr13, and the gene on 3BS mapped to the same region as the adult plant resistance gene Lr74 and other QTLs for Leaf Rust resistance. Kompetitive allele-specific PCR assay markers linked to the 2BS and 3BS regions were developed and should be useful for marker-based selection of these genes.
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adult plant Leaf Rust resistance in ac taber wheat maps to chromosomes 2bs and 3bs
Phytopathology, 2020Co-Authors: J A Kolmer, Matthew N Rouse, Kathryn M Turner, James A AndersonAbstract:AC Taber is a hard red spring wheat cultivar that has had long lasting resistance to the Leaf Rust fungus Puccinia triticina. The objective of this study was to determine the chromosome location of the Leaf Rust resistance genes in AC Taber. The Leaf Rust susceptible cultivar Thatcher was crossed with AC Taber to develop an F6 recombinant inbred line (RIL) population. The RILs and parents were evaluated for segregation of Leaf Rust resistance in five field plot tests and in two seedling tests to race BBBDB of P. triticina. A genetic map of the RIL population was developed using 90,000 single-nucleotide polymorphism (SNP) markers with the Illumina Infinium iSelect 90K wheat bead array. Quantitative trait loci (QTL) with significant effects for lower Leaf Rust severity in the field plot tests were found on chromosomes 2BS and 3BS. The same QTLs also had significant effects for lower infection type in seedlings to Leaf Rust race BBBDB. The gene on 2BS was the adult plant resistance gene Lr13, and the gene on 3BS mapped to the same region as the adult plant resistance gene Lr74 and other QTLs for Leaf Rust resistance. Kompetitive allele specific polymerase chain reaction (KASP) assay markers linked to the 2BS and 3BS regions were developed and should be useful for marker based selection of these genes.
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mapping and characterization of the new adult plant Leaf Rust resistance gene lr77 derived from santa fe winter wheat
Theoretical and Applied Genetics, 2018Co-Authors: J A Kolmer, Amy Bernardo, Guihua Bai, Shiaoman ChaoAbstract:A new gene for adult plant Leaf Rust resistance in wheat was mapped to chromosome 3BL. This gene was designated as Lr77. ‘Santa Fe’ is a hard red winter cultivar that has had long-lasting resistance to the Leaf Rust fungus, Puccinia triticina. The objective of this study was to determine the chromosome location of the adult plant Leaf Rust resistance in Santa Fe wheat. A partial backcross line of ‘Thatcher’ (Tc) wheat with adult plant Leaf Rust resistance derived from Santa Fe was crossed with Thatcher to develop a Thatcher//Tc*2/Santa Fe F6 recombinant inbred line (RIL) population. The RIL population and parental lines were evaluated for segregation of Leaf Rust resistance in three field plot tests and in an adult plant greenhouse test. A genetic map of the RIL population was constructed using 90,000 single-nucleotide polymorphism (SNP) markers with the Illumina Infinium iSelect 90K wheat bead array. A significant quantitative trait locus for reduction of Leaf Rust severity in all four tests was found on chromosome 3BL that segregated as a single adult plant resistance gene. The RILs with the allele from the resistant parent for SNP marker IWB10344 had lower Leaf Rust severity and a moderately resistant to moderately susceptible response compared to the susceptible RILs and Thatcher. The gene derived from Santa Fe on chromosome 3BL was designated as Lr77. Kompetitive allele-specific polymerase chain reaction assay markers linked to Lr77 on 3BL should be useful for selection of wheat germplasm with this gene.
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inheritance and bulked segregant analysis of Leaf Rust and stem Rust resistance in durum wheat genotypes
Phytopathology, 2017Co-Authors: Meriem Aoun, J A Kolmer, Matthew N Rouse, Worku Denbel Bulbula, Elias M Elias, Shiaoman Chao, Maricelis AcevedoAbstract:Leaf Rust, caused by Puccinia triticina, and stem Rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of Leaf Rust resistance (Lr) genes to P. triticina race BBBQJ and stem Rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight Leaf-Rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for Leaf Rust and stem Rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five Leaf-Rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized i...
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inheritance and bulked segregant analysis of Leaf Rust and stem Rust resistance in durum wheat genotypes
Phytopathology, 2017Co-Authors: Meriem Aoun, J A Kolmer, Matthew N Rouse, Worku Denbel Bulbula, Elias M Elias, Shiaoman Chao, Maricelis AcevedoAbstract:Leaf Rust, caused by Puccinia triticina, and stem Rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of Leaf Rust resistance (Lr) genes to P. triticina race BBBQJ and stem Rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight Leaf-Rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for Leaf Rust and stem Rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five Leaf-Rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.
Beat Keller - One of the best experts on this subject based on the ideXlab platform.
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characterization of lr75 a partial broad spectrum Leaf Rust resistance gene in wheat
Theoretical and Applied Genetics, 2017Co-Authors: Jyoti Singla, Linda Luthi, Thomas Wicker, Urmil Bansal, Simon G Krattinger, Beat KellerAbstract:Here, we describe a strategy to improve broad-spectrum Leaf Rust resistance by marker-assisted combination of two partial resistance genes. One of them represents a novel partial adult plant resistance gene, named Lr75. Leaf Rust caused by the fungal pathogen Puccinia triticina is a damaging disease of wheat (Triticum aestivum L.). The combination of several, additively-acting partial disease resistance genes has been proposed as a suitable strategy to breed wheat cultivars with high levels of durable field resistance. The Swiss winter wheat cultivar ‘Forno’ continues to show near-immunity to Leaf Rust since its release in the 1980s. This resistance is conferred by the presence of at least six quantitative trait loci (QTL), one of which is associated with the morphological trait Leaf tip necrosis. Here, we used a marker-informed strategy to introgress two ‘Forno’ QTLs into the Leaf Rust-susceptible Swiss winter wheat cultivar ‘Arina’. The resulting backcross line ‘ArinaLrFor’ showed markedly increased Leaf Rust resistance in multiple locations over several years. One of the introgressed QTLs, QLr.sfr-1BS, is located on chromosome 1BS. We developed chromosome 1B-specific microsatellite markers by exploiting the Illumina survey sequences of wheat cv. ‘Chinese Spring’ and mapped QLr.sfr-1BS to a 4.3 cM interval flanked by the SSR markers gwm604 and swm271. QLr.sfr-1BS does not share a genetic location with any of the described Leaf Rust resistance genes present on chromosome 1B. Therefore, QLr.sfr-1BS is novel and was designated as Lr75. We conclude that marker-assisted combination of partial resistance genes is a feasible strategy to increase broad-spectrum Leaf Rust resistance. The identification of Lr75 adds a novel and highly useful gene to the small set of known partial, adult plant Leaf Rust resistance genes.
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characterization of lr75 a partial broad spectrum Leaf Rust resistance gene in wheat
Theoretical and Applied Genetics, 2017Co-Authors: Jyoti Singla, Linda Luthi, Thomas Wicker, Urmil Bansal, Simon G Krattinger, Beat KellerAbstract:Key message Here, we describe a strategy to improve broad-spectrum Leaf Rust resistance by marker-assisted combination of two partial resistance genes. One of them represents a novel partial adult plant resistance gene, named Lr75.
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dissection of quantitative and durable Leaf Rust resistance in swiss winter wheat reveals a major resistance qtl in the lr34 chromosomal region
Theoretical and Applied Genetics, 2004Co-Authors: Thorsten Schnurbusch, G Schachermayr, M Winzeler, Monika Messmer, Sophie Paillard, A Schori, Beat KellerAbstract:The Swiss winter bread wheat cv. ‘Forno’ has a highly effective, durable and quantitative Leaf Rust (Puccinia triticina Eriks.) resistance which is associated with Leaf tip necrosis (LTN). We studied 240 single seed descent lines of an ‘Arina×Forno’ F5:7 population to identify and map quantitative trait loci (QTLs) for Leaf Rust resistance and LTN. Percentage of infected Leaf area (%) and the response to infection (RI) were evaluated in seven field trials and were transformed to the area under the disease progress curves (AUDPC). Using composite interval mapping and LOD >4.4, we identified eight chromosomal regions specifically associated with resistance. The largest and most consistent Leaf Rust resistance locus was identified on the short arm of chromosome 7D (32.6% of variance explained for AUDPC_% and 42.6% for AUDPC_RI) together with the major QTL for LTN (R2=55.6%) in the same chromosomal region as Lr34 (Xgwm295). A second major Leaf Rust resistance QTL (R2=28% and 31.5%, respectively) was located on chromosome arm 1BS close to Xgwm604 and was not associated with LTN. Additional minor QTLs for LTN (2DL, 3DL, 4BS and 5AL) and Leaf Rust resistance were identified. These latter QTLs might correspond to the Leaf Rust resistance genes Lr2 or Lr22 (2DS) and Lr14a (7BL).
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genetic analysis of durable Leaf Rust resistance in winter wheat
Theoretical and Applied Genetics, 2000Co-Authors: Monika Messmer, R Seyfarth, Catherine Feuillet, G Schachermayr, M Winzeler, M Keller, S Zanetti, Beat KellerAbstract:Quantitative resistance that delays the epidemic development of Leaf Rust in wheat is an important source for durable resistance breeding. The Swiss winter wheat variety ’Forno’ shows a high level of quantitative resistance against Leaf Rust. This resistance has been effective for more than 10 years and can therefore be considered to be durable. In order to map quantitative trait loci (QTL) for durable Leaf Rust resistance we analysed 204 F5 recombinant inbred lines (RILs) of the cross between the winter wheat ’Forno’ and the winter spelt ’Oberkulmer’ for their level of Leaf Rust resistance (LR) and Leaf tip necrosis (LTN) in four different environments. Both traits showed a continuous distribution and were significantly correlated (r=−0.5). Across environments we detected 8 QTL for Leaf Rust resistance (6 inherited from ’Forno’) and 10 QTL for the quantitative expression of LTN (6 inherited from ’Forno’). Of the 6 QTL responsible for the durable Leaf Rust resistance of ’Forno’, 1 major QTL coincided with a thaumatin locus on 7BL explaining 35% of the phenotypic variance. Four QTL for LR coincided with QTL for LTN. At these loci the alleles of ’Forno’ increased the level of resistance as well as the extent of LTN, indicating pleiotropy.
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development of a molecular marker for the adult plant Leaf Rust resistance gene lr35 in wheat
Theoretical and Applied Genetics, 1999Co-Authors: R Seyfarth, Catherine Feuillet, G Schachermayr, M Winzeler, Beat KellerAbstract:The objective of this work was to develop a marker for the adult plant Leaf Rust resistance gene Lr35. The Lr35 gene was originally introgressed into chromosome 2B from Triticum speltoides, a diploid relative of wheat. A segregating population of 96 F( 2 )plants derived from a cross between the resistant line ThatcherLr35 and the susceptible variety Frisal was analysed. Out of 80 RFLP probes previously mapped on wheat chromosome 2B, 51 detected a polymorphism between the parents of the cross. Three of them were completely linked with the resistance gene Lr35. The co-segregating probe BCD260 was converted into a PCR-based sequence-tagged-site (STS) marker. A set of 48 different breeding lines derived from several European breeding programs was tested with the STS marker. None of these lines has a donor for Lr35 in its pedigree and all of them reacted negatively with the STS marker. As no Leaf Rust races virulent on Lr35 have been found in different areas of the world, the STS marker for the Lr35 resistance gene is of great value to support the introgression of this gene in combination with other Leaf Rust (Lr) genes into breeding material by marker-assisted selection.
Ravi P Singh - One of the best experts on this subject based on the ideXlab platform.
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four consistent loci confer adult plant resistance to Leaf Rust in the durum wheat lines heller 1 and dunkler
Phytopathology, 2020Co-Authors: Chan Yuan, S A Herrerafoessel, Ravi P Singh, Julio Huertaespino, Susanne Dreisigacker, Mandeep S Randhawa, Demei Liu, Caixia LanAbstract:The durum wheat lines Heller#1 and Dunkler from the International Maize and Wheat Improvement Center Global Wheat Program showed moderate and stable adult plant resistance to Leaf Rust under high disease pressure over field environments in northwestern Mexico. Leaf Rust phenotyping was performed on two recombinant inbred line (RIL) populations derived from crosses of Heller#1 and Dunkler with the susceptible parent Atred#2, conducted under artificially induced Puccinia triticina epidemics in 2013, 2014, 2015, and 2016. The Atred#2 × Heller#1 and Atred#2 × Dunkler populations were genotyped by single nucleotide polymorphism (SNP) platforms and diversity arrays technology markers, respectively. Four Leaf Rust resistance quantitative trait loci were detected simultaneously in the two RIL populations: Lr46, QLr.cim-2BC, QLr.cim-5BL, and QLr.cim-6BL based on phenotypic data across all four crop seasons. They explained 11.7 to 46.8%, 7.2 to 26.1%, 8.4 to 24.1%, and 12.4 to 28.5%, respectively, of the phenotypic variation for Leaf Rust resistance in Atred#2 × Heller#1 and 16.3 to 56.6%, 6.7 to 15.7%, 4.1 to 10.1%, and 5.1 to 20.2% of the variation in the Atred#2 × Dunkler population. Only the resistance allele of QLr.cim-2BC was from the susceptible parent Atred#2, and resistance alleles at other loci came from the resistant parents Heller#1 and Dunkler. The SNP markers closely linked to Lr46 and QLr.cim-2BC were converted to kompetitive allele specific PCR markers for use in marker-assisted selection to improve Leaf Rust resistance through crosses with Heller#1 and Dunkler sources.
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identification and mapping of two adult plant Leaf Rust resistance genes in durum wheat
Molecular Breeding, 2019Co-Authors: Caixia Lan, S A Herrerafoessel, Julio Huertaespino, E S Lagudah, Bhoja R Basnet, Susanne Dreisigacker, Yong Ren, Ravi P SinghAbstract:The International Maize and Wheat Improvement Center (CIMMYT)-derived durum wheat line Quetru is moderately resistant to Leaf Rust and displays disease severities of 15–30% at the adult plant stage. Atil C2000 is moderately susceptible to Leaf Rust at adult plant stage and displays severities of 20–60%. These two durum wheats were crossed with the susceptible line Atred#1, and the respective recombinant inbred line populations were phenotyped for resistance to Leaf Rust in field trials in four environments in Mexico. The phenotypic segregation in the Atred#1 × Atil C2000 population was attributed to the pleiotropic adult plant resistance (APR) gene Lr46 based on the closest marker cslv46G22 located within 1.4 cM. Lr46 reduced Leaf Rust severity by 19.6–35.5%, and it was also identified in the Atred#1 × Quetru population, where it reduced Leaf Rust severity by 28.5–57.7% depending on the environment. We also identified a putatively novel, race-specific APR gene LrQ in Quetru that is located on the Chromosome 6BL and flanked by SNP markers AX-95144243 and AX-95155193. These two markers have been converted into kompetitive allele–specific PCR (KASP) markers and validated in the population. LrQ was ineffective at the seedling stage but reduced Leaf Rust severity by 37.3% on average over four field trials. The combination of LrQ and Lr46 resulted in significantly reduced Leaf Rust severity in the field and could be used to breed improved durum wheat varieties.
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overview and application of qtl for adult plant resistance to Leaf Rust and powdery mildew in wheat
Crop Science, 2014Co-Authors: Caixia Lan, Ravi P Singh, Garry M Rosewarne, Xinmin Chen, Xianchun XiaAbstract:Leaf Rust and powdery mildew, caused by Puccinia triticina and Blumeria graminis f. sp. tritici, respectively, are widespread fungal diseases of wheat (Triticum aestivum L.). Development of cultivars with durable resistance is crucially important for global wheat production. This paper reviews the progress of genetic study and application of adult plant resistance (APR) to wheat Leaf Rust and powdery mildew. Eighty Leaf Rust and 119 powdery mildew APR quantitative trait loci (QTL) have been reported on 16 and 21 chromosomes, respectively, in over 50 publications during the last 15 yr. More important, we found 11 loci located on chromosomes 1BS, 1BL, 2AL, 2BS (2), 2DL, 4DL, 5BL, 6AL, 7BL, and 7DS showing pleiotropic effects on resistance to Leaf Rust, stripe Rust, and powdery mildew. Among these, QTL on chromosomes 1BL, 4DL, and 7DS also correlate with Leaf tip necrosis. Fine mapping and cloning of these QTL will be achieved with the advent of cheaper high-throughput genotyping technologies. Germplasm carrying these potential resistance genes will be useful for developing cultivars with durable multidisease resistance. In addition to its non-NBS–LRR (nucleotide binding site–leucine rich repeat) structure, the senescence-like processes induced by Lr34 could be the reason for durability of resistance; however, more information is needed for a full understanding of the molecular mechanism related to durability. Adult plant resistance genes have been used by CIMMYT for more than 30 yr and have also been transferred to many Chinese wheat varieties through shuttle breeding.
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qtl characterization of resistance to Leaf Rust and stripe Rust in the spring wheat line francolin 1
Molecular Breeding, 2014Co-Authors: Caixia Lan, S A Herrerafoessel, Ravi P Singh, Julio Huertaespino, Garry M Rosewarne, Bhoja R Basnet, Yelun Zhang, Ennian YangAbstract:Growing resistant wheat varieties is a key method of controlling two important wheat diseases, Leaf Rust and stripe Rust. We analyzed quantitative trait loci (QTL) to investigate adult plant resistance (APR) to these Rusts, using 141 F5 RILs derived from the cross ‘Avocet-YrA/Francolin#1’. Phenotyping of Leaf Rust resistance was conducted during two seasons at Ciudad Obregon, Mexico, whereas stripe Rust was evaluated for two seasons in Toluca, Mexico, and one season in Chengdu, China. The genetic map was constructed with 581 markers, including diversity arrays technology and simple sequence repeat. Significant loci for reducing Leaf Rust severity were designated QLr.cim-1BL, QLr.cim-3BS.1, QLr.cim-3DC, and QLr.cim-7DS. The six QTL that reduced stripe Rust severity were designated QYr.cim-1BL, QYr.cim-2BS, QYr.cim-2DS, QYr.cim-3BS.2, QYr.cim-5AL, and QYr.cim-6AL. All loci were conferred by Francolin#1, with the exception of QYr.cim-2DS, QYr.cim-5AL, and QYr.cim-6AL, which were derived from Avocet-YrA. Closely linked markers indicated that the 1BL locus was the pleiotropic APR gene Lr46/Yr29. QYr.cim-2BS was a seedling resistance gene designated as YrF that conferred intermediate seedling reactions and moderate resistance at the adult plant stage in both Mexican and Chinese environments. Significant additive interactions were detected between the six QTL for stripe Rust, but not between the four QTL for Leaf Rust. Furthermore, we detected two new APR loci for Leaf Rust in common wheat: QLr.cim-3BS.1 and QLr.cim-7DS.
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global status of wheat Leaf Rust caused by puccinia triticina
Euphytica, 2011Co-Authors: J Huertaespino, Brent Mccallum, Ravi P Singh, R F Park, Silvia German, W Q Chen, S C Bhardwaj, Henriette GoyeauAbstract:Leaf Rust caused by Puccinia triticina is the most common and widely distributed of the three wheat Rusts. Losses from Leaf Rust are usually less damaging than those from stem Rust and stripe Rust, but Leaf Rust causes greater annual losses due to its more frequent and widespread occurrence. Yield losses from Leaf Rust are mostly due to reductions in kernel weight. Many laboratories worldwide conduct Leaf Rust surveys and virulence analyses. Most currently important races (pathotypes) have either evolved through mutations in existing populations or migrated from other, often unknown, areas. Several Leaf Rust resistance genes are cataloged, and high levels of slow Rusting adult plant resistance are available in high yielding CIMMYT wheats. This paper summarizes the importance of Leaf Rust in the main wheat production areas as reflected by yield losses, the complexity of virulence variation in pathogen populations, the role cultivars with race-specific resistance play in pathogen evolution, and the control measures currently practiced in various regions of the world.
Jyoti Singla - One of the best experts on this subject based on the ideXlab platform.
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characterization of lr75 a partial broad spectrum Leaf Rust resistance gene in wheat
Theoretical and Applied Genetics, 2017Co-Authors: Jyoti Singla, Linda Luthi, Thomas Wicker, Urmil Bansal, Simon G Krattinger, Beat KellerAbstract:Here, we describe a strategy to improve broad-spectrum Leaf Rust resistance by marker-assisted combination of two partial resistance genes. One of them represents a novel partial adult plant resistance gene, named Lr75. Leaf Rust caused by the fungal pathogen Puccinia triticina is a damaging disease of wheat (Triticum aestivum L.). The combination of several, additively-acting partial disease resistance genes has been proposed as a suitable strategy to breed wheat cultivars with high levels of durable field resistance. The Swiss winter wheat cultivar ‘Forno’ continues to show near-immunity to Leaf Rust since its release in the 1980s. This resistance is conferred by the presence of at least six quantitative trait loci (QTL), one of which is associated with the morphological trait Leaf tip necrosis. Here, we used a marker-informed strategy to introgress two ‘Forno’ QTLs into the Leaf Rust-susceptible Swiss winter wheat cultivar ‘Arina’. The resulting backcross line ‘ArinaLrFor’ showed markedly increased Leaf Rust resistance in multiple locations over several years. One of the introgressed QTLs, QLr.sfr-1BS, is located on chromosome 1BS. We developed chromosome 1B-specific microsatellite markers by exploiting the Illumina survey sequences of wheat cv. ‘Chinese Spring’ and mapped QLr.sfr-1BS to a 4.3 cM interval flanked by the SSR markers gwm604 and swm271. QLr.sfr-1BS does not share a genetic location with any of the described Leaf Rust resistance genes present on chromosome 1B. Therefore, QLr.sfr-1BS is novel and was designated as Lr75. We conclude that marker-assisted combination of partial resistance genes is a feasible strategy to increase broad-spectrum Leaf Rust resistance. The identification of Lr75 adds a novel and highly useful gene to the small set of known partial, adult plant Leaf Rust resistance genes.
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characterization of lr75 a partial broad spectrum Leaf Rust resistance gene in wheat
Theoretical and Applied Genetics, 2017Co-Authors: Jyoti Singla, Linda Luthi, Thomas Wicker, Urmil Bansal, Simon G Krattinger, Beat KellerAbstract:Key message Here, we describe a strategy to improve broad-spectrum Leaf Rust resistance by marker-assisted combination of two partial resistance genes. One of them represents a novel partial adult plant resistance gene, named Lr75.
S A Herrerafoessel - One of the best experts on this subject based on the ideXlab platform.
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four consistent loci confer adult plant resistance to Leaf Rust in the durum wheat lines heller 1 and dunkler
Phytopathology, 2020Co-Authors: Chan Yuan, S A Herrerafoessel, Ravi P Singh, Julio Huertaespino, Susanne Dreisigacker, Mandeep S Randhawa, Demei Liu, Caixia LanAbstract:The durum wheat lines Heller#1 and Dunkler from the International Maize and Wheat Improvement Center Global Wheat Program showed moderate and stable adult plant resistance to Leaf Rust under high disease pressure over field environments in northwestern Mexico. Leaf Rust phenotyping was performed on two recombinant inbred line (RIL) populations derived from crosses of Heller#1 and Dunkler with the susceptible parent Atred#2, conducted under artificially induced Puccinia triticina epidemics in 2013, 2014, 2015, and 2016. The Atred#2 × Heller#1 and Atred#2 × Dunkler populations were genotyped by single nucleotide polymorphism (SNP) platforms and diversity arrays technology markers, respectively. Four Leaf Rust resistance quantitative trait loci were detected simultaneously in the two RIL populations: Lr46, QLr.cim-2BC, QLr.cim-5BL, and QLr.cim-6BL based on phenotypic data across all four crop seasons. They explained 11.7 to 46.8%, 7.2 to 26.1%, 8.4 to 24.1%, and 12.4 to 28.5%, respectively, of the phenotypic variation for Leaf Rust resistance in Atred#2 × Heller#1 and 16.3 to 56.6%, 6.7 to 15.7%, 4.1 to 10.1%, and 5.1 to 20.2% of the variation in the Atred#2 × Dunkler population. Only the resistance allele of QLr.cim-2BC was from the susceptible parent Atred#2, and resistance alleles at other loci came from the resistant parents Heller#1 and Dunkler. The SNP markers closely linked to Lr46 and QLr.cim-2BC were converted to kompetitive allele specific PCR markers for use in marker-assisted selection to improve Leaf Rust resistance through crosses with Heller#1 and Dunkler sources.
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identification and mapping of two adult plant Leaf Rust resistance genes in durum wheat
Molecular Breeding, 2019Co-Authors: Caixia Lan, S A Herrerafoessel, Julio Huertaespino, E S Lagudah, Bhoja R Basnet, Susanne Dreisigacker, Yong Ren, Ravi P SinghAbstract:The International Maize and Wheat Improvement Center (CIMMYT)-derived durum wheat line Quetru is moderately resistant to Leaf Rust and displays disease severities of 15–30% at the adult plant stage. Atil C2000 is moderately susceptible to Leaf Rust at adult plant stage and displays severities of 20–60%. These two durum wheats were crossed with the susceptible line Atred#1, and the respective recombinant inbred line populations were phenotyped for resistance to Leaf Rust in field trials in four environments in Mexico. The phenotypic segregation in the Atred#1 × Atil C2000 population was attributed to the pleiotropic adult plant resistance (APR) gene Lr46 based on the closest marker cslv46G22 located within 1.4 cM. Lr46 reduced Leaf Rust severity by 19.6–35.5%, and it was also identified in the Atred#1 × Quetru population, where it reduced Leaf Rust severity by 28.5–57.7% depending on the environment. We also identified a putatively novel, race-specific APR gene LrQ in Quetru that is located on the Chromosome 6BL and flanked by SNP markers AX-95144243 and AX-95155193. These two markers have been converted into kompetitive allele–specific PCR (KASP) markers and validated in the population. LrQ was ineffective at the seedling stage but reduced Leaf Rust severity by 37.3% on average over four field trials. The combination of LrQ and Lr46 resulted in significantly reduced Leaf Rust severity in the field and could be used to breed improved durum wheat varieties.
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lr72 confers resistance to Leaf Rust in durum wheat cultivar atil c2000
Plant Disease, 2014Co-Authors: S A Herrerafoessel, Caixia Lan, V Calvosalazar, J Huertaespino, R P SinghAbstract:Abstract Leaf Rust, caused by Puccinia triticina (Pt), has become a globally important disease for durum wheat (Triticum turgidum subsp. durum) since the detection of race group BBG/BN, which renders ineffective a widely deployed seedling resistance gene present in several popular cultivars including Mexican cultivars Altar C84 and Atil C2000. The resistance gene continues to play a key role in protecting durum wheat against bread wheat–predominant races since virulence among this race group has not been found. We developed F3 and F5 mapping populations from a cross between Atil C2000 and the susceptible line Atred #1. Resistance was characterized by greenhouse seedling tests using three Pt races. Segregation tests indicated the presence of a single gene, which was mapped to the distal end of 7BS by bulk segregant analysis. The closest marker, wmc606, was located 5.5 cM proximal to the gene. No known Leaf Rust resistance genes are reported in this region; this gene was therefore designated as Lr72. The pr...
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qtl characterization of resistance to Leaf Rust and stripe Rust in the spring wheat line francolin 1
Molecular Breeding, 2014Co-Authors: Caixia Lan, S A Herrerafoessel, Ravi P Singh, Julio Huertaespino, Garry M Rosewarne, Bhoja R Basnet, Yelun Zhang, Ennian YangAbstract:Growing resistant wheat varieties is a key method of controlling two important wheat diseases, Leaf Rust and stripe Rust. We analyzed quantitative trait loci (QTL) to investigate adult plant resistance (APR) to these Rusts, using 141 F5 RILs derived from the cross ‘Avocet-YrA/Francolin#1’. Phenotyping of Leaf Rust resistance was conducted during two seasons at Ciudad Obregon, Mexico, whereas stripe Rust was evaluated for two seasons in Toluca, Mexico, and one season in Chengdu, China. The genetic map was constructed with 581 markers, including diversity arrays technology and simple sequence repeat. Significant loci for reducing Leaf Rust severity were designated QLr.cim-1BL, QLr.cim-3BS.1, QLr.cim-3DC, and QLr.cim-7DS. The six QTL that reduced stripe Rust severity were designated QYr.cim-1BL, QYr.cim-2BS, QYr.cim-2DS, QYr.cim-3BS.2, QYr.cim-5AL, and QYr.cim-6AL. All loci were conferred by Francolin#1, with the exception of QYr.cim-2DS, QYr.cim-5AL, and QYr.cim-6AL, which were derived from Avocet-YrA. Closely linked markers indicated that the 1BL locus was the pleiotropic APR gene Lr46/Yr29. QYr.cim-2BS was a seedling resistance gene designated as YrF that conferred intermediate seedling reactions and moderate resistance at the adult plant stage in both Mexican and Chinese environments. Significant additive interactions were detected between the six QTL for stripe Rust, but not between the four QTL for Leaf Rust. Furthermore, we detected two new APR loci for Leaf Rust in common wheat: QLr.cim-3BS.1 and QLr.cim-7DS.
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new slow Rusting Leaf Rust and stripe Rust resistance genes lr67 and yr46 in wheat are pleiotropic or closely linked
Theoretical and Applied Genetics, 2011Co-Authors: S A Herrerafoessel, Matthew J Hayden, Julio Huertaespino, E S Lagudah, Davinder Singh, H S Bariana, Ravi P SinghAbstract:The common wheat genotype ‘RL6077’ was believed to carry the gene Lr34/Yr18 that confers slow-Rusting adult plant resistance (APR) to Leaf Rust and stripe Rust but located to a different chromosome through inter-chromosomal reciprocal translocation. However, haplotyping using the cloned Lr34/Yr18 diagnostic marker and the complete sequencing of the gene indicated Lr34/Yr18 is absent in RL6077. We crossed RL6077 with the susceptible parent ‘Avocet’ and developed F3, F4 and F6 populations from photoperiod-insensitive F3 lines that were segregating for resistance to Leaf Rust and stripe Rust. The populations were characterized for Leaf Rust resistance at two Mexican sites, Cd. Obregon during the 2008–2009 and 2009–2010 crop seasons, and El Batan during 2009, and for stripe Rust resistance at Toluca, a third Mexican site, during 2009. The F3 population was also evaluated for stripe Rust resistance at Cobbitty, Australia, during 2009. Most lines had correlated responses to Leaf Rust and stripe Rust, indicating that either the same gene, or closely linked genes, confers resistance to both diseases. Molecular mapping using microsatellites led to the identification of five markers (Xgwm165, Xgwm192, Xcfd71, Xbarc98 and Xcfd23) on chromosome 4DL that are associated with this gene(s), with the closest markers being located at 0.4 cM. In a parallel study in Canada using a Thatcher × RL6077 F3 population, the same Leaf Rust resistance gene was designated as Lr67 and mapped to the same chromosomal region. The pleiotropic, or closely linked, gene derived from RL6077 that conferred stripe Rust resistance in this study was designated as Yr46. The slow-Rusting gene(s) Lr67/Yr46 can be utilized in combination with other slow-Rusting genes to develop high levels of durable APR to Leaf Rust and stripe Rust in wheat.