The Experts below are selected from a list of 144 Experts worldwide ranked by ideXlab platform
Laura Hartmann - One of the best experts on this subject based on the ideXlab platform.
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sequence defined glycopolymer segments presenting mannose synthesis and Lectin Binding affinity
Biomacromolecules, 2012Co-Authors: Daniela Ponader, Felix Wojcik, Figen Becerenbraun, Jens Dernedde, Laura HartmannAbstract:We present for the first time the synthesis of sequence-defined monodisperse glycopolymer segments via solid-phase polymer synthesis. Functional building blocks displaying alkyne moieties and hydrophilic ethylenedioxy units were assembled stepwise on solid phase. The resulting polymer segments were conjugated with mannose sugars via 1,3-dipolar cycloaddition. The obtained mono-, di-, and trivalent mannose structures were then subject to Con A Lectin Binding. Surface plasmon resonance studies showed a nonlinear increase in Binding regarding the number and spacing of sugar ligands. The results of Con A Lectin Binding assays indicate that the chemical composition of the polymeric scaffold strongly contributes to the Binding activities as well as the spacing between the ligands and the number of presented mannose units. Our approach now allows for the synthesis of highly defined glycooligomers and glycopolymers with a diversity of properties to investigate systematically multivalent effects of polymeric ligands.
Kazuya Yoshinaga - One of the best experts on this subject based on the ideXlab platform.
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Histochemical evaluation of postnatal Lectin-Binding sites in the mouse prostate.
Okajimas folia anatomica Japonica, 2020Co-Authors: Kentaro Sakuda, Ryoki Muragishi, Kazuya YoshinagaAbstract:The prostate is a male accessory genital gland that plays an essential role in reproductive function. To understand the cytological characteristics of differentiating prostatic cells, we used Lectin histochemistry combined with immunohistochemistry to examine the distribution of Lectin-Binding sites on prostatic cells during postnatal development in the mouse. During postnatal development, Hippeastrum Hybrid Lectin (HHL) Lectin reacted consistently with the luminal cells of all prostatic lobes (regions), whereas the Ricinus Communis Agglutinin I (RCA-I) and Soybean Agglutinin (SBA) Lectins showed remarkable differences with age, region, and cell type. We found that the Lectin-Binding pattern in differentiating prostatic cells acquired adult characteristics around 3 weeks after birth. The results indicate that prostatic cell differentiation during postnatal development in mice is characterized by the presence of cell- and region-specific Lectin-Binding sites in the prostate, suggesting that there may also be cellular and regional differences in their function. Furthermore, some Lectins (HHL, RCA-I, and SBA) could provide useful markers for research into cell differentiation and for the pathological evaluation of prostatic diseases or in the diagnosis of male infertility.
Daniela Ponader - One of the best experts on this subject based on the ideXlab platform.
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sequence defined glycopolymer segments presenting mannose synthesis and Lectin Binding affinity
Biomacromolecules, 2012Co-Authors: Daniela Ponader, Felix Wojcik, Figen Becerenbraun, Jens Dernedde, Laura HartmannAbstract:We present for the first time the synthesis of sequence-defined monodisperse glycopolymer segments via solid-phase polymer synthesis. Functional building blocks displaying alkyne moieties and hydrophilic ethylenedioxy units were assembled stepwise on solid phase. The resulting polymer segments were conjugated with mannose sugars via 1,3-dipolar cycloaddition. The obtained mono-, di-, and trivalent mannose structures were then subject to Con A Lectin Binding. Surface plasmon resonance studies showed a nonlinear increase in Binding regarding the number and spacing of sugar ligands. The results of Con A Lectin Binding assays indicate that the chemical composition of the polymeric scaffold strongly contributes to the Binding activities as well as the spacing between the ligands and the number of presented mannose units. Our approach now allows for the synthesis of highly defined glycooligomers and glycopolymers with a diversity of properties to investigate systematically multivalent effects of polymeric ligands.
Kentaro Sakuda - One of the best experts on this subject based on the ideXlab platform.
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Histochemical evaluation of postnatal Lectin-Binding sites in the mouse prostate.
Okajimas folia anatomica Japonica, 2020Co-Authors: Kentaro Sakuda, Ryoki Muragishi, Kazuya YoshinagaAbstract:The prostate is a male accessory genital gland that plays an essential role in reproductive function. To understand the cytological characteristics of differentiating prostatic cells, we used Lectin histochemistry combined with immunohistochemistry to examine the distribution of Lectin-Binding sites on prostatic cells during postnatal development in the mouse. During postnatal development, Hippeastrum Hybrid Lectin (HHL) Lectin reacted consistently with the luminal cells of all prostatic lobes (regions), whereas the Ricinus Communis Agglutinin I (RCA-I) and Soybean Agglutinin (SBA) Lectins showed remarkable differences with age, region, and cell type. We found that the Lectin-Binding pattern in differentiating prostatic cells acquired adult characteristics around 3 weeks after birth. The results indicate that prostatic cell differentiation during postnatal development in mice is characterized by the presence of cell- and region-specific Lectin-Binding sites in the prostate, suggesting that there may also be cellular and regional differences in their function. Furthermore, some Lectins (HHL, RCA-I, and SBA) could provide useful markers for research into cell differentiation and for the pathological evaluation of prostatic diseases or in the diagnosis of male infertility.
Jens Dernedde - One of the best experts on this subject based on the ideXlab platform.
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sequence defined glycopolymer segments presenting mannose synthesis and Lectin Binding affinity
Biomacromolecules, 2012Co-Authors: Daniela Ponader, Felix Wojcik, Figen Becerenbraun, Jens Dernedde, Laura HartmannAbstract:We present for the first time the synthesis of sequence-defined monodisperse glycopolymer segments via solid-phase polymer synthesis. Functional building blocks displaying alkyne moieties and hydrophilic ethylenedioxy units were assembled stepwise on solid phase. The resulting polymer segments were conjugated with mannose sugars via 1,3-dipolar cycloaddition. The obtained mono-, di-, and trivalent mannose structures were then subject to Con A Lectin Binding. Surface plasmon resonance studies showed a nonlinear increase in Binding regarding the number and spacing of sugar ligands. The results of Con A Lectin Binding assays indicate that the chemical composition of the polymeric scaffold strongly contributes to the Binding activities as well as the spacing between the ligands and the number of presented mannose units. Our approach now allows for the synthesis of highly defined glycooligomers and glycopolymers with a diversity of properties to investigate systematically multivalent effects of polymeric ligands.