The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform

Khalid M Alkharfy - One of the best experts on this subject based on the ideXlab platform.

  • hepatoprotective activity of Lepidium sativum seeds against d galactosamine lipopolysaccharide induced hepatotoxicity in animal model
    BMC Complementary and Alternative Medicine, 2016
    Co-Authors: Mohammad Raish, Fahad I Aljenoobi, Khalid M Alkharfy, Abdullah M Almohizea, Ajaz Ahmad, Syed Rizwan Ahamad, Kazi Mohsin, Mushtaq A Ansari
    Abstract:

    Fulminant hepatic failure (FHF) is clinical syndrome with very poor prognosis and high mortality there is urgent need for the development of safe and non-toxic hepatoprotective agents for the adequate management of hepatitis. Hepatoprotective effect of the Lepidium sativum ethanolic extract (LSEE) was assessed by D-galactosamine-induced/lipopolysaccharide (400 mg/kg and 30 μg/kg) liver damage model in rats. Hepatoprotective activity of LSEE (150 and 300 mg/kg) and silymarin on D-GalN/LPS induced FHF in rat was assessed using several liver function enzyme parameters. Antioxidant properties as antioxidant stress enzymes were assessed in hepatic Liver as well as mRNA expression of cytokines genes such as TNF-α, IL-6, and IL-10 and stress related genes iNOS and HO-1 were determined by RT-PCR. Protein expression of apoptotic genes were evaluated through western blot. MPO and NF-κB DNA-binding activity was analyzed by ELISA. The magnitude of hepatic impairment was investigated through histopathological evaluation. Marked amelioration of hepatic injuries by attenuation of serum and lipid peroxidation has been observed as comparable with silymarin (25 mg/kg p.o). D-GalN/LPS induced significant decrease in oxidative stress markers protein level, and albumin. LSEE significantly down-regulated the D-GalN/LPS induced pro-inflammatory cytokines TNFα and IL-6 mRNA expression in dose dependent fashion about 0.47 and 0.26 fold and up-regulates the IL-10 by 1.9 and 2.8 fold, respectively. While encourages hepatoprotective activity by down-regulating mRNA expression of iNOS and HO-1. MPO activity and NF-κB DNA-binding effect significantly increased and was mitigated by LSEE in a dose-dependent style as paralleled with silymarin. Our data suggests that pretreatment of LSEE down regulates the caspase 3 and up-regulates the BCl2 protein expression. The above findings revealed that Lepidium sativum has significant hepatoprotective activity.

  • effects of nigella sativa Lepidium sativum and trigonella foenum graecum on sildenafil disposition in beagle dogs
    European Journal of Drug Metabolism and Pharmacokinetics, 2015
    Co-Authors: Abdullah M Almohizea, Fahad I Aljenoobi, Khalid M Alkharfy, Abdul Ahad, Gamal M Elmaghraby, Saleh A Alsuwayeh
    Abstract:

    The present study was conducted to investigate the effects of some commonly used herbs namely Nigella sativa, Lepidium sativum and Trigonella foenum-graecum on the pharmacokinetics of sildenafil in beagle dogs. The study design involved four treatments in a non-balanced crossover design. Sildenafil was given one tablet 100 mg orally to each dog and blood samples were obtained. After a suitable washout period, animals were commenced on a specific herb treatment for 1 week. Blood samples were withdrawn at different time intervals and sildenafil was analyzed by HPLC method. Oral administration of Nigella sativa resulted in reduction of AUC0–∞, Cmax and t1/2 as compared to the control. Treatment of Lepidium sativum resulted in a significant reduction in the Cmax and AUC. There were no significant differences between the rests of the pharmacokinetic parameters relative to those of the control. For Trigonella foenum-graecum, the effects were similar to those obtained in case of Lepidium sativum. It was concluded that concurrent use of investigated herbs alters the pharmacokinetics of sildenafil. Co-administration of investigated herbs should be cautious since their concomitant use might result in decrease in sildenafil bioavailability.

  • effects of nigella sativa and Lepidium sativum on cyclosporine pharmacokinetics
    BioMed Research International, 2013
    Co-Authors: Fahad I Aljenoobi, Saleh A Alsuwayeh, Iqbal Muzaffar, Mohd Aftab Alam, Khalid M Alkharfy, Hesham M Korashy, Abdullah M Almohizea, Abdul Ahad, Mohd Raish
    Abstract:

    The present study was conducted to investigate the effects of Nigella sativa and Lepidium sativum on the pharmacokinetics of cyclosporine in rabbits. Two groups of animals were treated separately with Nigella sativa (200 mg/kg p.o.) or Lepidium sativum (150 mg/kg p.o.) for eight consecutive days. On the 8th day, cyclosporine (30 mg/kg p.o.) was administered to each group one hour after herbal treatment. Blood samples were withdrawn at different time intervals (0.0, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12, and 24 hrs) from marginal ear vein. Cyclosporine was analyzed using UPLC/MS method. The coadministration of Nigella sativa significantly decreased the and of cyclosporine; the change was observed by 35.5% and 55.9%, respectively (). Lepidium sativum did not produce any significant change in of cyclosporine, although its absorption was significantly delayed compared with control group. A remarkable change was observed in and of Lepidium sativum treated group. Our findings suggest that concurrent consumption of Nigella sativa and Lepidium sativum could alter the pharmacokinetics of cyclosporine at various levels.

  • Lepidium sativum but not nigella sativa affects carbamazepine disposition in an animal model
    Drug Metabolism Letters, 2013
    Co-Authors: Khalid M Alkharfy, Fahad I Aljenoobi, Saleh A Alsuwayeh, Mohd Aftab Alam, Hesham M Korashy, Abdullah M Almohizea, Raom A Khan, Iqbal Muzaffar
    Abstract:

    Efficacy and safety profile of a drug may be affected when concomitantly used with herbal medicines. The present study was conducted to investigate the effects of some commonly used herbal products viz. Nigella sativa (Black seed) and Lepidium sativum (Garden cress) on the pharmacokinetics of carbamazepine (CBZ), a narrow therapeutic index drug, in an animal model. In a control group, five rabbits received 40 mg/kg of CBZ orally and blood samples were withdrawn at different time intervals (0, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hrs) from a marginal ear vein. After a suitable washout period, an aqueous saline suspension of Nigella sativa (200 mg/kg) or Lepidium sativum (150 mg/kg) was given orally for eight days to the rabbits. On day eight, CBZ (40 mg/kg) was re-administrated orally and blood samples were collected using the same sampling scheme. Drug levels in plasma were determined by liquid chromatography and pharmacokinetic parameters were calculated using non-compartmental analysis. No significant difference was observed in the maximum concentration (C max ), area under concentration curve (AUC), half-life (T 1/2 ), clearance (Cl/F) and volume of distribution (V z /F) of CBZ following Nigella sativa treatment. Whereas, increased C max , absorption rate measured by the time to C max (T max ), and prolongation of the terminal elimination half-life (T 1/2 ) were observed after the co-administration with Lepidium sativum. Findings of the present study suggest that concurrent use of Lepidium sativum alters the pharmacokinetics of CBZ in an animal model. Further confirmation of these results in humans will warrant changes in CBZ dose and/or frequency before co-administration with these herbal medicines.

  • pharmacological basis for the medicinal use of Lepidium sativum in airways disorders
    Evidence-based Complementary and Alternative Medicine, 2012
    Co-Authors: Najeebur Rehman, Khalid M Alkharfy, Arifullah Khan, Anwarul Hassan Gilani
    Abstract:

    Lepidium sativum is widely used in folk medicine for treatment of hyperactive airways disorders, such as asthma, bronchitis and cough. The crude extract of Lepidium sativum (Ls.Cr) inhibited carbachol (CCh, 1 μM-) and K+ (80 mM-) induced contractions in a pattern similar to that of dicyclomine. Ls.Cr at 0.03 mg/mL produced a rightward parallel shift of CCh curves, followed by nonparallel shift at higher concentration (0.1 mg/mL), suppressing maximum response, similar to that caused by dicyclomine. Pretreatment of tissues with Ls.Cr (0.1–0.3 mg/mL) shifted Ca++ concentration-response curves (CRCs) to right, as produced by verapamil. Ls.Cr at low concentrations (0.03–0.1 mg/mL) caused leftward shift of isoprenaline-induced inhibitory CRCs, like that caused by rolipram, a phosphodiesterase (PDE) inhibitor. These results indicate that bronchodilatory effect of Lepidium sativum is mediated through a combination of anticholinergic, Ca++ antagonist and PDE inhibitory pathways, which provides sound mechanistic background for its medicinal use in the overactive airways disorders.

Meinhart H Zenk - One of the best experts on this subject based on the ideXlab platform.

  • seven imidazole alkaloids from Lepidium sativum
    Phytochemistry, 1998
    Co-Authors: Ulrich H Maier, Heidrun Gundlach, Meinhart H Zenk
    Abstract:

    Abstract The seeds of Lepidium sativum afforded five new dimeric imidazole alkaloids lepidine B, C, D, E and F in addition to the known imidazole alkaloid lepidine and two new monomeric imidazole alkaloids semilepidinoside A and B. Their structure were elucidated on the basis of spectroscopic evidence.

Anwarul Hassan Gilani - One of the best experts on this subject based on the ideXlab platform.

  • pharmacological basis for the medicinal use of Lepidium sativum in airways disorders
    Evidence-based Complementary and Alternative Medicine, 2012
    Co-Authors: Najeebur Rehman, Khalid M Alkharfy, Arifullah Khan, Anwarul Hassan Gilani
    Abstract:

    Lepidium sativum is widely used in folk medicine for treatment of hyperactive airways disorders, such as asthma, bronchitis and cough. The crude extract of Lepidium sativum (Ls.Cr) inhibited carbachol (CCh, 1 μM-) and K+ (80 mM-) induced contractions in a pattern similar to that of dicyclomine. Ls.Cr at 0.03 mg/mL produced a rightward parallel shift of CCh curves, followed by nonparallel shift at higher concentration (0.1 mg/mL), suppressing maximum response, similar to that caused by dicyclomine. Pretreatment of tissues with Ls.Cr (0.1–0.3 mg/mL) shifted Ca++ concentration-response curves (CRCs) to right, as produced by verapamil. Ls.Cr at low concentrations (0.03–0.1 mg/mL) caused leftward shift of isoprenaline-induced inhibitory CRCs, like that caused by rolipram, a phosphodiesterase (PDE) inhibitor. These results indicate that bronchodilatory effect of Lepidium sativum is mediated through a combination of anticholinergic, Ca++ antagonist and PDE inhibitory pathways, which provides sound mechanistic background for its medicinal use in the overactive airways disorders.

  • studies on antidiarrheal and antispasmodic activities of Lepidium sativum crude extract in rats
    Phytotherapy Research, 2012
    Co-Authors: Khalid M Alkharfy, Najeebur Rehman, Anwarul Hassan Gilani, Malik Hassan Mehmood
    Abstract:

    This study was aimed to provide the pharmacological basis for the medicinal use of Lepidium sativum in diarrhea using in vivo and in vitro assays. The seed extract of Lepidium sativum (Ls.Cr) at 100 and 300 mg/kg inhibited castor oil-induced diarrhea in rats. In isolated rat ileum, Ls.Cr (0.01–5 mg/mL) reversed carbachol (CCh, 1 µ m) and K+ (80 m m)-induced contractions with higher potency against CCh, similar to dicyclomine. Preincubation of rat ileum with a lower concentration of Ls.Cr (0.03 mg/mL) caused a rightward parallel shift in the concentration–response curves (CRCs) of CCh without suppression of the maximum response, while at the next higher concentration (0.1 mg/mL), it produced a non-parallel rightward shift with suppression of the maximum response, similar to that of dicyclomine. Ls.Cr shifted the CRCs of Ca++ to the right with suppression of the maximum response, similar to verapamil. These data suggest that Lepidium sativum seed extract possesses antidiarrheal and spasmolytic activities mediated possibly through dual blockade of muscarinic receptors and Ca++ channels, though additional mechanism(s) cannot be ruled out and this study explains its medicinal use in diarrhea and abdominal cramps. Copyright © 2011 John Wiley & Sons, Ltd.

  • prokinetic and laxative activities of Lepidium sativum seed extract with species and tissue selective gut stimulatory actions
    Journal of Ethnopharmacology, 2011
    Co-Authors: Khalid M Alkharfy, Anwarul Hassan Gilani, Malik Hassan Mehmood
    Abstract:

    Abstract Aim of the study To provide ethnopharmacological basis for the medicinal use of Lepidium sativum seeds in indigestion and constipation. Materials and methods The in vivo studies were conducted in mice, while isolated tissues of mouse, guinea-pig and rabbit were suspended in tissue bath to measure isotonic contractions. Results The aqueous-methanolic extract of Lepidium sativum seeds (Ls.Cr) at 30 and 100 mg/kg showed atropine-sensitive prokinetic and laxative activities in mice, which were partially sensitive to atropine. In isolated gut preparations of mouse and guinea-pig, Ls.Cr (0.1–1 mg/mL) caused a concentration-dependent stimulatory effects both in jejunum and ileum, which was blocked in the presence of atropine. In rabbit jejunum, the stimulant effect of Ls.Cr remained unchanged in the presence of atropine, pyrilamine or SB203186, while in rabbit ileum, the stimulatory effect was partially blocked by atropine. The Ls.Cr was more efficacious in gut preparations of rabbit than in guinea-pig or mouse. The phytochemical analysis of the plant extract detected alkaloids, saponins and anthraquinones as plant constituents. Conclusion This study showed the prokinetic and laxative effects of Lepidium sativum in mice, which were partially mediated through a cholinergic pathway. The in vitro spasmodic effect of the plant extract mediated through a similar mechanism with species and tissue-selectivity, provides a rationale for the medicinal use of the seeds of Lepidium sativum in indigestion and constipation, and suggests studying the plant extracts on more than one species to get the wider picture.

Bhumit N Patel - One of the best experts on this subject based on the ideXlab platform.

  • Lepidium sativum linn a current addition to the family of mucilage and its applications
    International Journal of Biological Macromolecules, 2014
    Co-Authors: Vipul D Prajapati, Pankaj M Maheriya, Girish K Jani, Prasant D Patil, Bhumit N Patel
    Abstract:

    Mucilage from the last decades has been found to be very attractive, interesting and useful in development of desired pharmaceutical dosage forms. Various applications of plant based mucilage have a wide potentiality in drug formulations. Lepidium sativum Linn. (family: Brassicaceae) is one of the mucilage containing fast growing, edible annual herb. Its various parts (roots, leaves and seeds) have been used to treat various human ailments. It mainly contains alkaloids, saponins, anthracene glycosides, carbohydrates, proteins, amino acids, flavanoids, and sterols as chief phytochemical constituents. Its seed extracts have been screened for various biological activities like hypotensive, anti-microbial, bronchodilator, hypoglycemic and allelopathic, whereas its seed coat mucilage has been isolated using different methods to make it effective excipient of desired functionality as a part of pharmaceutical applications. Through keen references of reported work on Lepidium sativum Linn., in this review, we have focused on its seed coat mucilage isolation methods, chemical constituents, pharmacological profile and versatile application of Lepidium sativum Linn.

Ulrich H Maier - One of the best experts on this subject based on the ideXlab platform.

  • seven imidazole alkaloids from Lepidium sativum
    Phytochemistry, 1998
    Co-Authors: Ulrich H Maier, Heidrun Gundlach, Meinhart H Zenk
    Abstract:

    Abstract The seeds of Lepidium sativum afforded five new dimeric imidazole alkaloids lepidine B, C, D, E and F in addition to the known imidazole alkaloid lepidine and two new monomeric imidazole alkaloids semilepidinoside A and B. Their structure were elucidated on the basis of spectroscopic evidence.