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R. B. Herberman - One of the best experts on this subject based on the ideXlab platform.
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Indirect Leukocyte Migration Inhibition Reactions to a 3-M KCI Extract of Lung Adenocarcinoma by Lung Cancer Patients 1,2
2016Co-Authors: I. Suslov, J L Mccoy, R. B. HerbermanAbstract:ABSTRACT-Mononuclear (MN) cells from the peripheral blood of lung cancer patients were tested for their ability to respond to a 3-M KCI extract of adenocarcinoma of lung with the use of an indirect Leukocyte Migration Inhibition (LMI) assay. Antigen-stimulated MN cell cultures were evaluated for Leukocyte inhib-itory factor production by their ability to inhibit the Migration of indicator polymorphonuclear cells from agarose droplets. When supernatants were prepared in conventional round-bottomed tubes (5X106 cells/tube), 25 of 44 (57%) lung cancer patients had positive indirect LMI responses to the 7661 antigen as compared to only 2 of the 30 (7%) normal donors. When supernatants were prepared in conical microtubes, with 10 times fewer MN cells, similar results were obtained. Patients with all histologic types of lung cancer had a similar incidence of reactivity, and reactivity of untreated patients did not appear t
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Leukocyte Migration Inhibition in Patients With Ewing's Sarcoma by 3-M Potassium Chloride Extracts of Fresh and Tissue-Cultured Ewing's Sarcomas 1. 2
2016Co-Authors: R. B. HerbermanAbstract:ABSTRACT-Leukocyte Migration Inhibition (LMI) assays were performed to detect cell·mediated immune reactions against nrmer-asscclated antigens (TAA) of Ewing's sarcoma. With the use of crude antigen preparations obtained by 3 M KClextractions of Iresh Ewing's sarcoma or of tissue culture cells derived from a pleural effusion of a Ewing's sarcoma patient, assays were per-lormed with Leukocytes from these patients, patients with other cancers, and normal donors. The results deinonstrated approx-imately 60 % or greater positive LMI reactivity in Ewing's sarcoma patients, as compared to less than 10 % reactlvity 01 normal donors, with the use 01 extracts of either fresh or tlssue-cultured Ewing's sarcoma cells. A lower proportion of positive reactivity was observed in patients with breast and lung cancer. Further speciflcity tests indicated that a smaller proportion 01 patients with Ewing's sarcoma had LMI reactivity with KCI extracts o
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Leukocyte Migration Inhibition and Lymphocyte Blastogenesis Responses in Breast Carcinoma Patients to Mouse Mammary Tumor Virus and to Virion gp52 Antigen and Rauscher Murine Leukemia Virus-Kirsten Sarcoma Virus gp69/71 Antigen 1
2016Co-Authors: R. B. HerbermanAbstract:ABSTRACT-Direct Leukocyte Migration Inhibition (LMI) and Iym-phocyte proliferation (LP) assays were conducted with leuko-cytes from patients with breast cancer, benign breast disease, and other types of cancer and from normal donors with the use of mouse mammary tumor virus (MuMTV), its major glycoprotein (gp52), murine leukemia virus (MuLV) glycoproteins (gp69/71), and normal nonvirion glycoproteins. With the use of LMI, leuko-cytes from 13 of 15 (83%) breast carcinoma patients and 3 of 5 benign breast disease patients reacted with MuMTV, whereas Leukocytes from 1 of 16 (6%) normal donors and 0 of 9 patients with other cancers reacted. LMI with gp52 was positive in 39 of 60 (65%) patients with breast cancer, 8 of 15 (53%) with fibrocystic disease, 2 of 11 (18%) with fibroadenoma, 1 of 15 (7%) normal donors, and 3 of 15 (29%) patients with other cancers. Specificity studies with gp69/71 preparations of Rauscher MuLV and Kirste
Jonathan Black - One of the best experts on this subject based on the ideXlab platform.
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hypersensitivity to metallic biomaterials a review of Leukocyte Migration Inhibition assays
Biomaterials, 2000Co-Authors: Nadim J Hallab, Joshua J Jacobs, Jonathan BlackAbstract:Abstract Metal hypersensitivity is a well-established phenomenon occurring in a variety of domestic and workplace settings. Degradation products of metallic biomaterials may mediate metal hypersensitivity. However, little is known about the short- and long-term pharmacodynamics and bioavailability of circulating metal degradation products in vivo. Mechanisms by which in vivo metal sensitivity reactions occur have not been well characterized and the degree to which metal sensitivity may be a predisposing factor for eliciting an overaggressive immune response remains clinically unpredictable. In vitro Leukocyte Migration Inhibition assays have been used for investigating cell-mediated hypersensitivity reactions to biomaterial and biomaterial degradation products. This review provides a historical and technical summary of four in vitro techniques used for determination of Leukocyte Migration activity: (1) membrane Migration or Boyden chamber, (2) capillary tube, (3) Leukocyte Migration using agarose technique, and (4) collagen gels. It is difficult to determine which, if any, of these techniques is singularly best suited for the investigation of suspected biomaterial-related symptoms in patients. However, Boyden chamber membrane Migration testing is recommended for clinical investigations, principally because a high degree of standardized investigator independent materials and methodologies is necessary for compiling and comparing the results of patients tested at various times over the length of an extended study. Ultimately, in vitro Migration Inhibition testing has the potential to provide a reliable means for predicting some complications and thus enhancing the outcome for patients receiving metallic implants. Continuing improvements in Migration Inhibition testing methods, used alone or in combination with other immunologic assays, will likely improve assessment of patients susceptible to biomaterial antigen-induced delayed-type hypersensitivity responses.
Nadim J Hallab - One of the best experts on this subject based on the ideXlab platform.
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hypersensitivity to metallic biomaterials a review of Leukocyte Migration Inhibition assays
Biomaterials, 2000Co-Authors: Nadim J Hallab, Joshua J Jacobs, Jonathan BlackAbstract:Abstract Metal hypersensitivity is a well-established phenomenon occurring in a variety of domestic and workplace settings. Degradation products of metallic biomaterials may mediate metal hypersensitivity. However, little is known about the short- and long-term pharmacodynamics and bioavailability of circulating metal degradation products in vivo. Mechanisms by which in vivo metal sensitivity reactions occur have not been well characterized and the degree to which metal sensitivity may be a predisposing factor for eliciting an overaggressive immune response remains clinically unpredictable. In vitro Leukocyte Migration Inhibition assays have been used for investigating cell-mediated hypersensitivity reactions to biomaterial and biomaterial degradation products. This review provides a historical and technical summary of four in vitro techniques used for determination of Leukocyte Migration activity: (1) membrane Migration or Boyden chamber, (2) capillary tube, (3) Leukocyte Migration using agarose technique, and (4) collagen gels. It is difficult to determine which, if any, of these techniques is singularly best suited for the investigation of suspected biomaterial-related symptoms in patients. However, Boyden chamber membrane Migration testing is recommended for clinical investigations, principally because a high degree of standardized investigator independent materials and methodologies is necessary for compiling and comparing the results of patients tested at various times over the length of an extended study. Ultimately, in vitro Migration Inhibition testing has the potential to provide a reliable means for predicting some complications and thus enhancing the outcome for patients receiving metallic implants. Continuing improvements in Migration Inhibition testing methods, used alone or in combination with other immunologic assays, will likely improve assessment of patients susceptible to biomaterial antigen-induced delayed-type hypersensitivity responses.
Rathinasamy Sheela Devi - One of the best experts on this subject based on the ideXlab platform.
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effect of methanol induced oxidative stress on the neuroimmune system of experimental rats
Chemico-Biological Interactions, 2006Co-Authors: Narayanaperumal Jeya Parthasarathy, Ramasundaram Sri Kumar, Sundaramahalingam Manikandan, Govindarajulu Sathya Narayanan, Raman Vijaya Kumar, Rathinasamy Sheela DeviAbstract:It is well known that the nervous system has increased susceptibility to methanol intoxication. The present study reveals the effect of methanol intoxication on antioxidant status, lipid peroxidation and DNA integrity in hypothalamic-pituitary-adrenal (HPA) axis organs and spleen. Non-specific and specific immune functions were analyzed. In addition, open field behavior, plasma corticosterone level and blood methanol level were estimated. Male Wistar albino rats were intoxicated with methanol (2.37 g/kg b.wt., i.p.) for 1 day, 15 and 30 days. Administration of methanol showed significant increase in enzymatic (superoxide dismutase, catalase, glutathione peroxidase), non-enzymatic (reduced glutathione and Vitamin C) antioxidants and lipid peroxidation (LPO) in hypothalamus and adrenal gland of day 1 group. However, decrease in enzymatic and non-enzymatic antioxidants with concomitant increase in LPO level were observed in 15 and 30 days groups. Plasma corticosterone level was significantly increased in day 1 and 15 days groups whereas, 30 days methanol intoxication group showed considerable decrease in corticosterone level compared with control animals. Cell-mediated immune response of footpad thickness was significantly decreased with an increased Leukocyte Migration Inhibition. Humoral immune response of antibody titers was elevated in methanol-intoxicated groups. Neutrophil functions, adherence and phagocytic index (PI) were found to be significantly decreases. Furthermore, significant increase in the avidity index and nitro blue tetrozolium reduction was observed in the methanol exposed animals. Day 1 methanol exposed group showed increased PI compared to the control ones. Methanol exposure for 30 days showed an increased DNA fragmentation in the hypothalamus, adrenal glands, and spleen. In conclusion, exposure to methanol-induced oxidative stress disturbs the HPA-axis function altering the level of corticosterone, which lead to varied non-specific and specific immune response in experimental rats.
Kunal Saha - One of the best experts on this subject based on the ideXlab platform.
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why relapse occurs in pb leprosy patients after adequate mdt despite they are mitsuda reactive lessons form convit s experiment on bacteria clearing capacity of lepromin induced granuloma
International Journal of Leprosy and Other Mycobacterial Diseases, 1998Co-Authors: S Chaudhuri, A Mukherjee, S K Hajra, Bibhuti Saha, D Chattapadhya, B Mazumder, Kunal SahaAbstract:It is amazing how after years of scientific research and therapeutic progress many simple and basic questions about protective immunity against Mycobacterium leprae remain unanswered. Although the World Health Organization (WHO) has recommended short-term multidrug therapy (WHO/MDT) for the treatment of paucibacillary (PB) leprosy patients, from time to time several workers from different parts of the globe have reported inadequate clinical responses in a few tuberculoid and indeterminate leprosy patients following adequate WHO/MDT despite the fact that they are Mitsuda responsive. A few borderline tuberculoid patients harbor acid-fast bacilli (AFB) in their nerves for many years even though they become clinically inactive following MDT, a fact which has been ignored by many leprosy field workers. Keeping these patients in mind, we have attempted to investigate the cause of the persistence of AFB in PB cases and have looked into the question of why Mitsuda positivity in tuberculoid and indeterminate leprosy patients, as well as in healthy contacts, is not invariably a guarantee for protectivity against the leprosy bacilli. We have: a) analyzed the histological features of lepromin-induced granulomas, b) studied the bacteria-clearing capacity of the macrophages within such granulomas, and c) studied the in vitro Leukocyte Migration Inhibition factor released by the blood Leukocytes of these subjects when M. leprae sonicates have been used as an elicitor. The results of these three tests in the three groups of subjects have been compared and led us to conclude that the bacteria-clearing capacity of the macrophages within lepromin-induced granuloma (positive CCB test) may be taken as an indicator of the capability of elimination of leprosy bacilli and protective immunity against the disease. This important macrophage function is not invariably present in all tuberculoid and indeterminate leprosy patients or in all contacts even though they are Mitsuda responsive and are able to show a positive Leukocyte Migration Inhibition (LMI) test. It is likely but not certain that this deficit of the macrophage is genetically predetermined and persists after completion of short-term WHO/MDT. Thus, after discontinuation of treatment slow-growing, persisting M. leprae multiply within macrophages leading to relapse.
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immunotherapy of lepromin negative borderline leprosy patients with low dose convit vaccine as an adjunct to multidrug therapy a six year follow up study in calcutta
International Journal of Leprosy and Other Mycobacterial Diseases, 1997Co-Authors: S Chaudhury, S K Hajra, A Mukerjee, Bibhuti Saha, V Majumdar, D Chattapadhya, Kunal SahaAbstract:The present report, which describes management of lepromin-negative borderline leprosy patients with low-dose Convit vaccine, is an extension of our earlier study on the treatment of lepromatous leprosy patients with low-dose Convit vaccine as an adjunct to multidrug therapy (MDT). The test Group I, consisting of 50 lepromin-negative, borderline leprosy patients, were given low-dose Convit vaccine plus MDT. The control group II consisted of 25 lepromin-negative, borderline leprosy patients given BCG vaccination plus MDT and 25 lepromin-negative, borderline leprosy patients given killed Mycobacterium leprae (human) vaccine plus MDT. The control group III consisted of 50 lepromin-positive, borderline leprosy patients not given any immunostimulation but given only MDT. Depending upon the lepromin unresponsiveness, the patients were given one to four inoculations of the various antileprosy vaccines and were followed up every 3 months for 2 years for clinical, bacteriological and immunological outcome. All patients belonging to the test and control groups showed clinical cure and bacteriological negativity within 2 years. However, immunologic potentiation, assessed by lepromin testing and the Leukocyte Migration Inhibition test (LMIT), was better in the test patients receiving low-dose Convit vaccine plus MDT than in the control patients receiving BCG vaccine plus MDT or killed M. leprae vaccine plus MDT or MDT alone. But the capacity of clearance bacteria (CCB) test from the lepromin granuloma showed poor bacterial clearance in the test patients. However, there was no relapse during 6 years of follow up. Two mid-borderline (BB) patients had severe reversal reactions with lagophthalmos and wrist drop during immunotherapy despite being given low-dose Convit vaccine.