The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Wenlei Wang - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of miRNAs involved in adventitious branches formation of Gracilaria Lichenoides in vitro
Journal of Applied Phycology, 2017Co-Authors: Wenlei Wang, Zhaokai Wang, Xiangzhi Lin, Huanqin Li, Baishan FangAbstract:The main morphological response of Gracilaria Lichenoides explants to wounding in vitro was the direct production of adventitious branches (ABs) within a short period rather than callus formation, similar to other seaweeds. However, the underlying mechanism remains unclear because of the lack of genome information. MicroRNAs (miRNAs) play a role in plant growth and development by targeting mRNAs for translational repression or cleavage. Here, we investigated small RNA-mediated regulation of ABs formation by examining the post-transcriptional profiles of different treatment samples of G. Lichenoides using the Illumina sequencing platform. The results showed that miR156g-3p, miR2597, miR3447-3p, miR169j-3p and other miRNAs families play significant roles in ABs formation in G. Lichenoides through their predicted targets, including phospholipase D (PLD), auxin response factor (ARF), protein phosphatase 2C (PP2C), CTR1 and adenylate isopentenyltransferase (IPT). In addition, we identified 30 putative unique Gracilariales miRNAs in two small RNA libraries that may be involved in ABs induction. Profiling of miRNAs and their target genes of G. Lichenoides provide new insight on miRNAs networks involved in ABs formation in seaweed.
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the effect of polar auxin transport on adventitious branches formation in gracilaria Lichenoides in vitro
Physiologia Plantarum, 2016Co-Authors: Huanqin Li, Wenlei Wang, Baishan Fang, Fang Zhang, Zhaokai WangAbstract:Seaweed tissue culture (STC) is an important micropropagation tool that has been applied for strain improvement, micropropagation and genetic engineering. Because the mechanisms associated with STC are poorly understood, its application to these organisms lags far behind that of tissue culture propagation of higher plants. Auxin, calcium (Ca2+ ) and hydrogen peroxide (H2 O2 ) fluxes all play key roles during plant growth and development. In this study, we therefore measured indole-3-acetic acid, Ca2+ and H2 O2 fluxes of Gracilaria Lichenoides explants during adventitious branches (ABs) formation for the first time using noninvasive micro-test technology. We confirmed that polar auxin transport (PAT) also occurs in the marine red alga G. Lichenoides. We additionally found that N-1-naphthylphthalamic acid may suppress auxin efflux via ABCB1 transporters and then inhibit ABs formation from the apical region of G. Lichenoides segments. The involvement of Ca2+ and H2 O2 fluxes in PAT-mediated AB formation in G. Lichenoides was also investigated. We propose that complex feedback among Ca2+ , H2 O2 and auxin signaling and response systems may occur during ABs polar formation in G. Lichenoides explants, similar to that in higher plants. Our results provide innovative insights that should aid future elucidation of mechanisms operative during STC.
Jimenez J Joaquin - One of the best experts on this subject based on the ideXlab platform.
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Phototherapy for Pityriasis Lichenoides in the Pediatric Population: A Review of the Published Literature
American Journal of Clinical Dermatology, 2016Co-Authors: Eric L Maranda, Megan Smith, Austin Huy Nguyen, Lawrence A Schachner, Vivek N. Patel, Jimenez J JoaquinAbstract:Background Pityriasis Lichenoides (PL) is a dermatologic disorder that manifests in either the acute (pityriasis Lichenoides et varioliformis acuta) or the chronic form (pityriasis Lichenoides chronica, also known as parapsoriasis chronica). Traditional first-line therapy consists of corticosteroids or antibiotics; however, these treatments are often accompanied with multiple side effects and may be ineffective. Objective The goal of this study was to review the use of phototherapy for treating PL in the pediatric population. Materials and methods We performed a systematic review of the literature in the National Library of Medicine’s PubMed database and the SCOPUS database discussing phototherapy for treatment of PL in the pediatric population. The following search terms were used: ‘pityriasis Lichenoides’, ‘pityriasis Lichenoides chronica’, ‘pityriasis Lichenoides et varioliformis acuta’, and ‘febrile ulceronecrotic Mucha-Habermann disease’. Results The systematic search and screening of articles resulted in 14 articles including a total of 64 patients with PL treated with phototherapy. Three different modalities were utilized, with five studies using broadband ultraviolet B (BB-UVB) radiation, nine studies utilizing narrowband UVB (NB-UVB), and two studies employing psoralen with ultraviolet A (PUVA) therapy. Overall, the use of BB-UVB had an initial clearance rate of 89.6 % with 23.1 % recurrence, whereas NB-UVB cleared 73 % of the lesions with no recurrence, and PUVA therapy initially cleared 83 % of the lesions with 60 % recurrence. The side-effect profiles were similar and revealed limited toxicity. Conclusion Phototherapy shows promising results and a favorable side-effect profile in the treatment of PL. Ultimately, large randomized controlled trials are needed to determine optimal treatments.
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phototherapy for pityriasis Lichenoides in the pediatric population a review of the published literature
American Journal of Clinical Dermatology, 2016Co-Authors: Eric L Maranda, Megan Smith, Austin Huy Nguyen, V Patel, Lawrence A Schachner, Jimenez J JoaquinAbstract:Background Pityriasis Lichenoides (PL) is a dermatologic disorder that manifests in either the acute (pityriasis Lichenoides et varioliformis acuta) or the chronic form (pityriasis Lichenoides chronica, also known as parapsoriasis chronica). Traditional first-line therapy consists of corticosteroids or antibiotics; however, these treatments are often accompanied with multiple side effects and may be ineffective.
Baishan Fang - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of miRNAs involved in adventitious branches formation of Gracilaria Lichenoides in vitro
Journal of Applied Phycology, 2017Co-Authors: Wenlei Wang, Zhaokai Wang, Xiangzhi Lin, Huanqin Li, Baishan FangAbstract:The main morphological response of Gracilaria Lichenoides explants to wounding in vitro was the direct production of adventitious branches (ABs) within a short period rather than callus formation, similar to other seaweeds. However, the underlying mechanism remains unclear because of the lack of genome information. MicroRNAs (miRNAs) play a role in plant growth and development by targeting mRNAs for translational repression or cleavage. Here, we investigated small RNA-mediated regulation of ABs formation by examining the post-transcriptional profiles of different treatment samples of G. Lichenoides using the Illumina sequencing platform. The results showed that miR156g-3p, miR2597, miR3447-3p, miR169j-3p and other miRNAs families play significant roles in ABs formation in G. Lichenoides through their predicted targets, including phospholipase D (PLD), auxin response factor (ARF), protein phosphatase 2C (PP2C), CTR1 and adenylate isopentenyltransferase (IPT). In addition, we identified 30 putative unique Gracilariales miRNAs in two small RNA libraries that may be involved in ABs induction. Profiling of miRNAs and their target genes of G. Lichenoides provide new insight on miRNAs networks involved in ABs formation in seaweed.
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the effect of polar auxin transport on adventitious branches formation in gracilaria Lichenoides in vitro
Physiologia Plantarum, 2016Co-Authors: Huanqin Li, Wenlei Wang, Baishan Fang, Fang Zhang, Zhaokai WangAbstract:Seaweed tissue culture (STC) is an important micropropagation tool that has been applied for strain improvement, micropropagation and genetic engineering. Because the mechanisms associated with STC are poorly understood, its application to these organisms lags far behind that of tissue culture propagation of higher plants. Auxin, calcium (Ca2+ ) and hydrogen peroxide (H2 O2 ) fluxes all play key roles during plant growth and development. In this study, we therefore measured indole-3-acetic acid, Ca2+ and H2 O2 fluxes of Gracilaria Lichenoides explants during adventitious branches (ABs) formation for the first time using noninvasive micro-test technology. We confirmed that polar auxin transport (PAT) also occurs in the marine red alga G. Lichenoides. We additionally found that N-1-naphthylphthalamic acid may suppress auxin efflux via ABCB1 transporters and then inhibit ABs formation from the apical region of G. Lichenoides segments. The involvement of Ca2+ and H2 O2 fluxes in PAT-mediated AB formation in G. Lichenoides was also investigated. We propose that complex feedback among Ca2+ , H2 O2 and auxin signaling and response systems may occur during ABs polar formation in G. Lichenoides explants, similar to that in higher plants. Our results provide innovative insights that should aid future elucidation of mechanisms operative during STC.
Zhaokai Wang - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of miRNAs involved in adventitious branches formation of Gracilaria Lichenoides in vitro
Journal of Applied Phycology, 2017Co-Authors: Wenlei Wang, Zhaokai Wang, Xiangzhi Lin, Huanqin Li, Baishan FangAbstract:The main morphological response of Gracilaria Lichenoides explants to wounding in vitro was the direct production of adventitious branches (ABs) within a short period rather than callus formation, similar to other seaweeds. However, the underlying mechanism remains unclear because of the lack of genome information. MicroRNAs (miRNAs) play a role in plant growth and development by targeting mRNAs for translational repression or cleavage. Here, we investigated small RNA-mediated regulation of ABs formation by examining the post-transcriptional profiles of different treatment samples of G. Lichenoides using the Illumina sequencing platform. The results showed that miR156g-3p, miR2597, miR3447-3p, miR169j-3p and other miRNAs families play significant roles in ABs formation in G. Lichenoides through their predicted targets, including phospholipase D (PLD), auxin response factor (ARF), protein phosphatase 2C (PP2C), CTR1 and adenylate isopentenyltransferase (IPT). In addition, we identified 30 putative unique Gracilariales miRNAs in two small RNA libraries that may be involved in ABs induction. Profiling of miRNAs and their target genes of G. Lichenoides provide new insight on miRNAs networks involved in ABs formation in seaweed.
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the effect of polar auxin transport on adventitious branches formation in gracilaria Lichenoides in vitro
Physiologia Plantarum, 2016Co-Authors: Huanqin Li, Wenlei Wang, Baishan Fang, Fang Zhang, Zhaokai WangAbstract:Seaweed tissue culture (STC) is an important micropropagation tool that has been applied for strain improvement, micropropagation and genetic engineering. Because the mechanisms associated with STC are poorly understood, its application to these organisms lags far behind that of tissue culture propagation of higher plants. Auxin, calcium (Ca2+ ) and hydrogen peroxide (H2 O2 ) fluxes all play key roles during plant growth and development. In this study, we therefore measured indole-3-acetic acid, Ca2+ and H2 O2 fluxes of Gracilaria Lichenoides explants during adventitious branches (ABs) formation for the first time using noninvasive micro-test technology. We confirmed that polar auxin transport (PAT) also occurs in the marine red alga G. Lichenoides. We additionally found that N-1-naphthylphthalamic acid may suppress auxin efflux via ABCB1 transporters and then inhibit ABs formation from the apical region of G. Lichenoides segments. The involvement of Ca2+ and H2 O2 fluxes in PAT-mediated AB formation in G. Lichenoides was also investigated. We propose that complex feedback among Ca2+ , H2 O2 and auxin signaling and response systems may occur during ABs polar formation in G. Lichenoides explants, similar to that in higher plants. Our results provide innovative insights that should aid future elucidation of mechanisms operative during STC.
Huanqin Li - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of miRNAs involved in adventitious branches formation of Gracilaria Lichenoides in vitro
Journal of Applied Phycology, 2017Co-Authors: Wenlei Wang, Zhaokai Wang, Xiangzhi Lin, Huanqin Li, Baishan FangAbstract:The main morphological response of Gracilaria Lichenoides explants to wounding in vitro was the direct production of adventitious branches (ABs) within a short period rather than callus formation, similar to other seaweeds. However, the underlying mechanism remains unclear because of the lack of genome information. MicroRNAs (miRNAs) play a role in plant growth and development by targeting mRNAs for translational repression or cleavage. Here, we investigated small RNA-mediated regulation of ABs formation by examining the post-transcriptional profiles of different treatment samples of G. Lichenoides using the Illumina sequencing platform. The results showed that miR156g-3p, miR2597, miR3447-3p, miR169j-3p and other miRNAs families play significant roles in ABs formation in G. Lichenoides through their predicted targets, including phospholipase D (PLD), auxin response factor (ARF), protein phosphatase 2C (PP2C), CTR1 and adenylate isopentenyltransferase (IPT). In addition, we identified 30 putative unique Gracilariales miRNAs in two small RNA libraries that may be involved in ABs induction. Profiling of miRNAs and their target genes of G. Lichenoides provide new insight on miRNAs networks involved in ABs formation in seaweed.
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the effect of polar auxin transport on adventitious branches formation in gracilaria Lichenoides in vitro
Physiologia Plantarum, 2016Co-Authors: Huanqin Li, Wenlei Wang, Baishan Fang, Fang Zhang, Zhaokai WangAbstract:Seaweed tissue culture (STC) is an important micropropagation tool that has been applied for strain improvement, micropropagation and genetic engineering. Because the mechanisms associated with STC are poorly understood, its application to these organisms lags far behind that of tissue culture propagation of higher plants. Auxin, calcium (Ca2+ ) and hydrogen peroxide (H2 O2 ) fluxes all play key roles during plant growth and development. In this study, we therefore measured indole-3-acetic acid, Ca2+ and H2 O2 fluxes of Gracilaria Lichenoides explants during adventitious branches (ABs) formation for the first time using noninvasive micro-test technology. We confirmed that polar auxin transport (PAT) also occurs in the marine red alga G. Lichenoides. We additionally found that N-1-naphthylphthalamic acid may suppress auxin efflux via ABCB1 transporters and then inhibit ABs formation from the apical region of G. Lichenoides segments. The involvement of Ca2+ and H2 O2 fluxes in PAT-mediated AB formation in G. Lichenoides was also investigated. We propose that complex feedback among Ca2+ , H2 O2 and auxin signaling and response systems may occur during ABs polar formation in G. Lichenoides explants, similar to that in higher plants. Our results provide innovative insights that should aid future elucidation of mechanisms operative during STC.