The Experts below are selected from a list of 20238 Experts worldwide ranked by ideXlab platform
Jun Miao - One of the best experts on this subject based on the ideXlab platform.
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rapid isolation of single malaria parasite infected red blood cells by cell sorting
Nature Protocols, 2011Co-Authors: Jun Miao, Liwang CuiAbstract:Malaria research often requires isolation of individually infected red blood cells (RBCs) or of a homogenous parasite population derived from a single parasite (clone). Traditionally, isolation of individual, parasitized RBCs or parasite cloning is achieved by limiting dilution or micromanipulation. This protocol describes a method for more efficient cloning of the malaria parasite; the method uses a cell sorter to rapidly isolate Plasmodium falciparum-infected RBCs singly. By gating the parameters of forward-angle Light Scatter and side-angle Light Scatter in a cell sorter, singly infected RBCs can be isolated and automatically deposited into a 96-well culture plate within 1 min. Including a Percoll purification step; the entire procedure to seed a 96-well plate with singly infected RBCs can take <40 min. This highly efficient single-cell sorting protocol should be useful for cloning of both laboratory parasite populations from genetic manipulation experiments and clinical samples.
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rapid isolation of single malaria parasite infected red blood cells by cell sorting
Nature Protocols, 2011Co-Authors: Jun MiaoAbstract:Malaria research often requires isolation of individually infected red blood cells (RBCs) or a homogenous parasite population derived from a single parasite (clone). Traditionally, isolation of individual, parasitized RBCs or parasite cloning is achieved by limiting dilution or micromanipulation. This protocol describes a method for more efficient cloning of the malaria parasite, which uses a cell sorter to rapidly isolate singly Plasmodium falciparum-infected RBCs. By gating the parameters of forward angle Light Scatter (FSC) and side angle Light Scatter (SSC) in a cell sorter, singly-infected RBCs can be isolated and automatically deposited into a 96-well culture plate within one minute. To include a Percoll purification step, the entire procedure to seed a 96-well plate with singly-infected RBCs takes less than 40 min. This highly efficient single-cell sorting protocol should be useful for cloning of both laboratory parasite populations from genetic manipulation experiments and clinical samples.
Keith H Baratz - One of the best experts on this subject based on the ideXlab platform.
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corneal haze determined by confocal microscopy 2 years after descemet stripping with endothelial keratoplasty for fuchs corneal dystrophy
Archives of Ophthalmology, 2012Co-Authors: Keith H Baratz, Leo J Maguire, Jay W Mclaren, Sanjay V PatelAbstract:Objective To quantify corneal Light Scatter and its relationship to vision after Descemet stripping with endothelial keratoplasty (DSEK). Methods Eyes with Fuchs corneal dystrophy were examined before and 1, 3, 6, 12, and 24 months after DSEK. Outcome measures were high- and low-contrast visual acuity, contrast sensitivity, and forward Light Scatter. Corneal reflectivity (backScatter), expressed in Scatter units (SU), was quantified using in vivo confocal microscopy. Results Comparing 49 study eyes with 35 normal eyes, the mean (SD) corneal subepithelial layer was more reflective than normal before (2325 [613] vs 1208 [287] SU, P Conclusions In Fuchs corneal dystrophy, the corneal subepithelial region is a more important source of forward Light Scatter than the DSEK interface. Subepithelial haze improves more in younger patients and is associated with improvement in forward Light Scatter. Clinical Relevance Visual function after DSEK is affected by residual haze in the anterior host cornea more so than the surgical interface. Haze, which likely is experienced as glare disability, improves after surgical intervention but improves more in younger patients.
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the effect of corneal Light Scatter on vision after descemet stripping with endothelial keratoplasty
Archives of Ophthalmology, 2009Co-Authors: Sanjay V Patel, Keith H Baratz, David O Hodge, Leo J Maguire, Jay W MclarenAbstract:OBJECTIVE To establish an association between corneal Light Scatter and vision after Descemet stripping with endothelial keratoplasty (DSEK). METHODS Twenty eyes of patients with Fuchs endothelial dystrophy were examined before and at 1, 3, and 6 months after DSEK in a prospective study. Main outcome measures were high-contrast best-corrected visual acuity, intraocular forward Light Scatter, and corneal backScatter. RESULTS One eye was excluded because of endothelial graft failure within 1 month. Best-corrected visual acuity improved at 3 months after DSEK (mean [standard deviation], 0.31 [0.20] logarithm of the minimum angle of resolution [logMAR]; Snellen equivalent, 20/41) relative to before DSEK (0.46 [0.26] logMAR; Snellen equivalent, 20/58; P = .03). Posterior corneal backScatter decreased 1 month after DSEK (P < .001), but backScatter from the anterior, middle, and posterior cornea did not return to normal by 6 months (P < or = .02). At 6 months, best-corrected visual acuity correlated with recipient age (r = 0.84, P < .001) and with intraocular forward Light Scatter (r = 0.67, P < .001); forward Light Scatter also correlated with recipient age (r = 0.67, P < .001). CONCLUSIONS Six months after DSEK, corneal Light Scatter remained greater in eyes with Fuchs endothelial dystrophy than in normal eyes and correlated with recipient age and visual acuity. Recipient age might be the best preoperative predictor of vision after DSEK.
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Scattered Light and visual function in a randomized trial of deep lamellar endothelial keratoplasty and penetrating keratoplasty
American Journal of Ophthalmology, 2008Co-Authors: Sanjay V Patel, David O Hodge, Jay W Mclaren, Keith H BaratzAbstract:Purpose To compare vision, intraocular forward Light Scatter and corneal backScatter between deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) for endothelial dysfunction. Design A randomized clinical trial. Methods Thirteen eyes (12 patients) were randomized to DLEK with a 9 mm scleral incision, and 15 eyes (14 patients) were randomized to PK. The primary outcome was high-contrast best spectacle-corrected visual acuity (BSCVA) at 12 months after surgery; intraocular forward Light Scatter and corneal backScatter were measured at one, three, six, and 12 months after surgery. Results BSCVA at 12 months was 0.34 ± 0.16 logMAR (logarithm of the minimum angle of resolution) for DLEK and 0.25 ± 0.21 logMAR for PK (P = .23; minimum detectable difference at 12 months was 0.23 logMAR). The change in postoperative forward Light Scatter after DLEK correlated with the change in BSCVA (r = −0.66; P Conclusions BSCVA was similar at one year after DLEK and PK. Improvement in BSCVA after DLEK correlated with decreasing forward Light Scatter. Increased backScatter after DLEK originated not only from the posterior cornea (interface) but also from the host cornea, which might limit visual outcomes after posterior lamellar keratoplasty.
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Scattered Light and visual function in a randomized trial of deep lamellar endothelial keratoplasty and penetrating keratoplasty
American Journal of Ophthalmology, 2008Co-Authors: Sanjay V Patel, David O Hodge, Jay W Mclaren, Keith H BaratzAbstract:Purpose To compare vision, intraocular forward Light Scatter and corneal backScatter between deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) for endothelial dysfunction. Design A randomized clinical trial. Methods Thirteen eyes (12 patients) were randomized to DLEK with a 9 mm scleral incision, and 15 eyes (14 patients) were randomized to PK. The primary outcome was high-contrast best spectacle-corrected visual acuity (BSCVA) at 12 months after surgery; intraocular forward Light Scatter and corneal backScatter were measured at one, three, six, and 12 months after surgery. Results BSCVA at 12 months was 0.34 ± 0.16 logMAR (logarithm of the minimum angle of resolution) for DLEK and 0.25 ± 0.21 logMAR for PK ( P = .23; minimum detectable difference at 12 months was 0.23 logMAR). The change in postoperative forward Light Scatter after DLEK correlated with the change in BSCVA ( r = −0.66; P P ≤ .005), at one through 12 months in the middle third ( P P ≤ .02) of the cornea. BackScatter after DLEK did not return to normal through 12 months ( P Conclusions BSCVA was similar at one year after DLEK and PK. Improvement in BSCVA after DLEK correlated with decreasing forward Light Scatter. Increased backScatter after DLEK originated not only from the posterior cornea (interface) but also from the host cornea, which might limit visual outcomes after posterior lamellar keratoplasty.
Sanjay V Patel - One of the best experts on this subject based on the ideXlab platform.
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corneal haze determined by confocal microscopy 2 years after descemet stripping with endothelial keratoplasty for fuchs corneal dystrophy
Archives of Ophthalmology, 2012Co-Authors: Keith H Baratz, Leo J Maguire, Jay W Mclaren, Sanjay V PatelAbstract:Objective To quantify corneal Light Scatter and its relationship to vision after Descemet stripping with endothelial keratoplasty (DSEK). Methods Eyes with Fuchs corneal dystrophy were examined before and 1, 3, 6, 12, and 24 months after DSEK. Outcome measures were high- and low-contrast visual acuity, contrast sensitivity, and forward Light Scatter. Corneal reflectivity (backScatter), expressed in Scatter units (SU), was quantified using in vivo confocal microscopy. Results Comparing 49 study eyes with 35 normal eyes, the mean (SD) corneal subepithelial layer was more reflective than normal before (2325 [613] vs 1208 [287] SU, P Conclusions In Fuchs corneal dystrophy, the corneal subepithelial region is a more important source of forward Light Scatter than the DSEK interface. Subepithelial haze improves more in younger patients and is associated with improvement in forward Light Scatter. Clinical Relevance Visual function after DSEK is affected by residual haze in the anterior host cornea more so than the surgical interface. Haze, which likely is experienced as glare disability, improves after surgical intervention but improves more in younger patients.
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the effect of corneal Light Scatter on vision after descemet stripping with endothelial keratoplasty
Archives of Ophthalmology, 2009Co-Authors: Sanjay V Patel, Keith H Baratz, David O Hodge, Leo J Maguire, Jay W MclarenAbstract:OBJECTIVE To establish an association between corneal Light Scatter and vision after Descemet stripping with endothelial keratoplasty (DSEK). METHODS Twenty eyes of patients with Fuchs endothelial dystrophy were examined before and at 1, 3, and 6 months after DSEK in a prospective study. Main outcome measures were high-contrast best-corrected visual acuity, intraocular forward Light Scatter, and corneal backScatter. RESULTS One eye was excluded because of endothelial graft failure within 1 month. Best-corrected visual acuity improved at 3 months after DSEK (mean [standard deviation], 0.31 [0.20] logarithm of the minimum angle of resolution [logMAR]; Snellen equivalent, 20/41) relative to before DSEK (0.46 [0.26] logMAR; Snellen equivalent, 20/58; P = .03). Posterior corneal backScatter decreased 1 month after DSEK (P < .001), but backScatter from the anterior, middle, and posterior cornea did not return to normal by 6 months (P < or = .02). At 6 months, best-corrected visual acuity correlated with recipient age (r = 0.84, P < .001) and with intraocular forward Light Scatter (r = 0.67, P < .001); forward Light Scatter also correlated with recipient age (r = 0.67, P < .001). CONCLUSIONS Six months after DSEK, corneal Light Scatter remained greater in eyes with Fuchs endothelial dystrophy than in normal eyes and correlated with recipient age and visual acuity. Recipient age might be the best preoperative predictor of vision after DSEK.
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Scattered Light and visual function in a randomized trial of deep lamellar endothelial keratoplasty and penetrating keratoplasty
American Journal of Ophthalmology, 2008Co-Authors: Sanjay V Patel, David O Hodge, Jay W Mclaren, Keith H BaratzAbstract:Purpose To compare vision, intraocular forward Light Scatter and corneal backScatter between deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) for endothelial dysfunction. Design A randomized clinical trial. Methods Thirteen eyes (12 patients) were randomized to DLEK with a 9 mm scleral incision, and 15 eyes (14 patients) were randomized to PK. The primary outcome was high-contrast best spectacle-corrected visual acuity (BSCVA) at 12 months after surgery; intraocular forward Light Scatter and corneal backScatter were measured at one, three, six, and 12 months after surgery. Results BSCVA at 12 months was 0.34 ± 0.16 logMAR (logarithm of the minimum angle of resolution) for DLEK and 0.25 ± 0.21 logMAR for PK (P = .23; minimum detectable difference at 12 months was 0.23 logMAR). The change in postoperative forward Light Scatter after DLEK correlated with the change in BSCVA (r = −0.66; P Conclusions BSCVA was similar at one year after DLEK and PK. Improvement in BSCVA after DLEK correlated with decreasing forward Light Scatter. Increased backScatter after DLEK originated not only from the posterior cornea (interface) but also from the host cornea, which might limit visual outcomes after posterior lamellar keratoplasty.
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Scattered Light and visual function in a randomized trial of deep lamellar endothelial keratoplasty and penetrating keratoplasty
American Journal of Ophthalmology, 2008Co-Authors: Sanjay V Patel, David O Hodge, Jay W Mclaren, Keith H BaratzAbstract:Purpose To compare vision, intraocular forward Light Scatter and corneal backScatter between deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) for endothelial dysfunction. Design A randomized clinical trial. Methods Thirteen eyes (12 patients) were randomized to DLEK with a 9 mm scleral incision, and 15 eyes (14 patients) were randomized to PK. The primary outcome was high-contrast best spectacle-corrected visual acuity (BSCVA) at 12 months after surgery; intraocular forward Light Scatter and corneal backScatter were measured at one, three, six, and 12 months after surgery. Results BSCVA at 12 months was 0.34 ± 0.16 logMAR (logarithm of the minimum angle of resolution) for DLEK and 0.25 ± 0.21 logMAR for PK ( P = .23; minimum detectable difference at 12 months was 0.23 logMAR). The change in postoperative forward Light Scatter after DLEK correlated with the change in BSCVA ( r = −0.66; P P ≤ .005), at one through 12 months in the middle third ( P P ≤ .02) of the cornea. BackScatter after DLEK did not return to normal through 12 months ( P Conclusions BSCVA was similar at one year after DLEK and PK. Improvement in BSCVA after DLEK correlated with decreasing forward Light Scatter. Increased backScatter after DLEK originated not only from the posterior cornea (interface) but also from the host cornea, which might limit visual outcomes after posterior lamellar keratoplasty.
Geunyoung Yoon - One of the best experts on this subject based on the ideXlab platform.
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epithelium and bowman s layer thickness and Light Scatter in keratoconic cornea evaluated using ultrahigh resolution optical coherence tomography
Journal of Biomedical Optics, 2012Co-Authors: Rahul Yadav, Ranjini Kottaiyan, Kamran Ahmad, Geunyoung YoonAbstract:A custom-developed ultrahigh resolution optical coherence tomography with an axial resolution of 1.1 μm in corneal tissue was used to characterize thickness and Light Scatter of the epithelium and Bowman's layer in keratoconic (KC) cornea noninvasively. A 4-mm wide vertical corneal section around the apex in nine KC and eight normal eyes was imaged in vivo. The epithelium and Bowman's layer were visualized and their thickness profiles were quantified. Scatter was quantified based on the sensitivity normalized mean signal intensity distribution. Average mean thickness of the epithelium and Bowman's layer in KC eyes was significantly smaller (p<0.05) than the normal eyes. The epithelium thickness variation across a central 3-mm cornea was significantly larger in KC eyes than in normal eyes. The Scatter in KC eyes was significantly increased only for Bowman's layer. The changes observed in this study could improve our understanding of the underlying disease mechanism of KC and can provide new indications for early disease diagnosis.
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epithelium and bowman s layer thickness and Light Scatter in keratoconic cornea evaluated using ultrahigh resolution optical coherence tomography
Journal of Biomedical Optics, 2012Co-Authors: Rahul Yadav, Ranjini Kottaiyan, Kamran Ahmad, Geunyoung YoonAbstract:A custom-developed ultrahigh resolution optical coherence tomography with an axial resolution of 1.1 μm in corneal tissue was used to characterize thickness and Light Scatter of the epithelium and Bowman's layer in keratoconic (KC) cornea noninvasively. A 4-mm wide vertical corneal section around the apex in nine KC and eight normal eyes was imaged in vivo. The epithelium and Bowman's layer were visualized and their thickness profiles were quantified. Scatter was quantified based on the sensitivity normalized mean signal intensity distribution. Average mean thickness of the epithelium and Bowman's layer in KC eyes was significantly smaller ðp < 0.05Þ than the normal eyes. The epithelium thickness variation across a central 3-mm cornea was significantly larger in KC eyes than in normal eyes. The Scatter in KC eyes was significantly increased only for Bowman's layer. The changes observed in this study could improve our understanding of the underlying disease mechanism of KC and can provide new indications for early disease diagnosis. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). (DOI:
Dongsheng Xu - One of the best experts on this subject based on the ideXlab platform.
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quantitative determination of neutrophil vcs parameters by the coulter automated hematology analyzer
American Journal of Clinical Pathology, 2005Co-Authors: Fernando Chaves, Bethany Tierno, Dongsheng XuAbstract:We studied the clinical usefulness of the VCS parameters (mean channels of cell volume, conductivity, and Light Scatter) in reactive neutrophils for predicting acute bacterial infection, which are obtained by the Coulter LH 750 hematology analyzer (Beckman Coulter, Fullerton, CA) during automated differential counts. Peripheral blood samples from 69 patients with positive blood cultures for bacteria and 35 control subjects were studied. We observed a significant increase in the mean channel of neutrophil volume (MNV) from septic patients compared with control subjects (156 ± 13.5 vs 143 ± 4.8; P < .001). The mean channel of neutrophil Light Scatter was decreased significantly in patients (140 ± 10.1 vs 146 ± 7.3; P = .002). An elevation of the MNV was associated with a higher WBC count and percentage of neutrophils and was present even in patients who did not have leukocytosis or neutrophilia. With a cutoff of 150 for the MNV, a specificity of 91% and sensitivity of 70% were achieved. As a quantitative, objective, and more sensitive parameter, we believe that the MNV has a potential to be an additional indicator for acute bacterial infection.
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quantitative determination of neutrophil vcs parameters by the coulter automated hematology analyzer new and reliable indicators for acute bacterial infection
American Journal of Clinical Pathology, 2005Co-Authors: Fernando Chaves, Bethany Tierno, Dongsheng XuAbstract:We studied the clinical usefulness of the VCS parameters (mean channels of cell volume, conductivity, and Light Scatter) in reactive neutrophils for predicting acute bacterial infection, which are obtained by the Coulter LH 750 hematology analyzer (Beckman Coulter, Fullerton, CA) during automated differential counts. Peripheral blood samples from 69 patients with positive blood cultures for bacteria and 35 control subjects were studied. We observed a significant increase in the mean channel of neutrophil volume (MNV) from septic patients compared with control subjects (156 ± 13.5 vs 143 ± 4.8; P < .001). The mean channel of neutrophil Light Scatter was decreased significantly in patients (140 ± 10.1 vs 146 ± 7.3; P = .002). An elevation of the MNV was associated with a higher WBC count and percentage of neutrophils and was present even in patients who did not have leukocytosis or neutrophilia. With a cutoff of 150 for the MNV, a specificity of 91% and sensitivity of 70% were achieved. As a quantitative, objective, and more sensitive parameter, we believe that the MNV has a potential to be an additional indicator for acute bacterial infection. The correct and timely diagnosis of severe acute infectious processes, such as septicemia, is extremely important for proper patient management. However, given the overlapping clinical signs and symptoms with those of other acute critical medical conditions, achieving a high level of certainty while diagnosing septicemia can be a difficult challenge in which the laboratory has a crucial role providing invaluable information. The tests most often ordered in this scenario are the CBC count with differential, blood culture, and often a manual differential count. Although an automated hematology analyzer can readily