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Joseph P. Peacock - One of the best experts on this subject based on the ideXlab platform.

  • pyrosequencing reveals high temperature cellulolytic microbial consortia in great boiling spring after in situ lignocellulose enrichment
    PLOS ONE, 2013
    Co-Authors: Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85°C in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture.

  • Pyrosequencing Reveals High-Temperature Cellulolytic Microbial Consortia in Great Boiling Spring after In Situ
    2012
    Co-Authors: Lignocellulose Enrichment, Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85uC in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture

Brian P Hedlund - One of the best experts on this subject based on the ideXlab platform.

  • pyrosequencing reveals high temperature cellulolytic microbial consortia in great boiling spring after in situ lignocellulose enrichment
    PLOS ONE, 2013
    Co-Authors: Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85°C in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture.

  • Pyrosequencing Reveals High-Temperature Cellulolytic Microbial Consortia in Great Boiling Spring after In Situ
    2012
    Co-Authors: Lignocellulose Enrichment, Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85uC in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture

Duane P Moser - One of the best experts on this subject based on the ideXlab platform.

  • pyrosequencing reveals high temperature cellulolytic microbial consortia in great boiling spring after in situ lignocellulose enrichment
    PLOS ONE, 2013
    Co-Authors: Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85°C in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture.

  • Pyrosequencing Reveals High-Temperature Cellulolytic Microbial Consortia in Great Boiling Spring after In Situ
    2012
    Co-Authors: Lignocellulose Enrichment, Joseph P. Peacock, Jessica K Cole, Senthil K Murugapiran, Jeremy A Dodsworth, Jenny C Fisher, Duane P Moser, Brian P Hedlund
    Abstract:

    To characterize high-temperature cellulolytic microbial communities, two Lignocellulosic Substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85uC in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by Lignocellulosic Substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture

Carlos E Rodriguezrodriguez - One of the best experts on this subject based on the ideXlab platform.

  • design of an optimized biomixture for the degradation of carbofuran based on pesticide removal and toxicity reduction of the matrix
    Environmental Science and Pollution Research, 2015
    Co-Authors: Juan Salvador Chinpampillo, Karla Ruizhidalgo, Mario Masismora, Elizabeth Carazorojas, Carlos E Rodriguezrodriguez
    Abstract:

    Pesticide biopurification systems contain a biologically active matrix (biomixture) responsible for the accelerated elimination of pesticides in wastewaters derived from pest control in crop fields. Biomixtures have been typically prepared using the volumetric composition 50:25:25 (Lignocellulosic Substrate/humic component/soil); nonetheless, formal composition optimization has not been performed so far. Carbofuran is an insecticide/nematicide of high toxicity widely employed in developing countries. Therefore, the composition of a highly efficient biomixture (composed of coconut fiber, compost, and soil, FCS) for the removal of carbofuran was optimized by means of a central composite design and response surface methodology. The volumetric content of soil and the ratio coconut fiber/compost were used as the design variables. The performance of the biomixture was assayed by considering the elimination of carbofuran, the mineralization of 14C-carbofuran, and the residual toxicity of the matrix, as response variables. Based on the models, the optimal volumetric composition of the FCS biomixture consists of 45:13:42 (coconut fiber/compost/soil), which resulted in minimal residual toxicity and ∼99 % carbofuran elimination after 3 days. This optimized biomixture considerably differs from the standard 50:25:25 composition, which remarks the importance of assessing the performance of newly developed biomixtures during the design of biopurification systems.

  • degradation of carbofuran by trametes versicolor in rice husk as a potential Lignocellulosic Substrate for biomixtures from mineralization to toxicity reduction
    Process Biochemistry, 2014
    Co-Authors: Karla Ruizhidalgo, Juan Salvador Chinpampillo, Mario Masismora, Elizabeth R Carazo, Carlos E Rodriguezrodriguez
    Abstract:

    Abstract Biopurification systems for the treatment of pesticide-containing wastewaters contain a biomixture constituted by low-cost materials (soil, Lignocellulosic Substrates and humic components). In particular, the use of bioaugmented Lignocellulosic materials as part of the biomixture components has been scarcely studied. The use of the fungus Trametes versicolor grown in rice husk (RH) was evaluated as an option of Lignocellulosic bioaugmented Substrate of potential application in biomixtures for carbofuran (CFN) elimination. RH supported growth and activity of the fungus, as evidenced visually and by laccase activity. The system was able to degrade 55.1% CFN in 34 d with a half-life of 29.9 d, and produced 3-hydroxycarbofuran (but not 3-ketocarbofuran) as a transformation product. This metabolite was successfully eliminated during the process. Moreover, a small fraction of CFN was mineralized (7.4%) within 64 d. Toxicological evaluation revealed a marked detoxification during the process, achieving an 89% decrease in the toxicity after 34 d. Overall performance of the bioaugmented Substrate indicates that RH/T. versicolor is an interesting option to take into account in the design of biomixtures for pesticide degradation.

  • re inoculation strategies enhance the degradation of emerging pollutants in fungal bioaugmentation of sewage sludge
    Bioresource Technology, 2014
    Co-Authors: Carlos E Rodriguezrodriguez, Sara Rodriguezmozaz, Daniel Lucas, Damia Barcelo, E Baron, Pablo Gagoferrero, Daniel Molinsdelgado, Ethel Eljarrat, Silvia M Diazcruz
    Abstract:

    Abstract The use of Trametes versicolor has been partially successful in the removal of some pharmaceuticals from sewage sludge in laboratory-scale biopile systems. The application of two strategies for the re-inoculation of biomass was assessed during the fungal bioaugmentation of non-sterile sludge (42-d treatment) as an approach to improve the elimination of pharmaceuticals and other groups of emerging pollutants. Globally, the re-inoculation of biopiles with blended mycelium exerted a major effect on the removal of pharmaceuticals (86%), brominated-flame-retardants (81%) and UV filters (80%) with respect to the re-inoculation with additional Lignocellulosic Substrate colonized by the fungus (69–67–22%). The performance was better than that of the analogous non-re-inoculated systems that were assayed previously for the removal of pharmaceuticals. The results demonstrate the ability of T. versicolor to remove a wide spectrum of emerging micropollutants under non-sterile conditions, while re-inoculation appears to be a useful step to improve the fungal treatment of sludge.

  • solid phase treatment with the fungus trametes versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge
    Bioresource Technology, 2011
    Co-Authors: Carlos E Rodriguezrodriguez, Damia Barcelo, Aleksandra Jelic, Marta Llorca, Marinella Farre, Gloria Caminal, Mira Petrovic, Teresa Vicent
    Abstract:

    For safe biosolid-land-applying, sludge should be contaminant-free. However, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. An alternative treatment with the fungus Trametes versicolor was applied in sterile solid-phase systems consisting of sludge and a Lignocellulosic Substrate. Fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test (ND24). Fourteen out of 43 analyzed pharmaceuticals were found in the raw sludge. After treatment, phenazone, bezafibrate, fenofibrate, cimetidine, clarithromycin, sulfamethazine and atenolol were completely removed, while removals between 42% and 80% were obtained for the remaining pharmaceuticals. Toxicological analyses (Daphnia magna, Vibrio fischeri and seed germination) showed an important reduction in sludge toxicity after treatment. Results suggest that a solid-phase treatment with T. versicolor may reduce the ecotoxicological impact of micropollutants present in sewage sludge. This is the first report of a fungal-approach for elimination of emerging pollutants from biosolids.

Michel Aplincourt - One of the best experts on this subject based on the ideXlab platform.

  • copper sorption onto a Lignocellulosic Substrate from wheat bran impregnated with a lipophilic tetraazamacrocycle
    Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2006
    Co-Authors: Stephanie Sayen, Francoise Chuburu, Emmanuel Guillon, Michel Aplincourt, Henri Handel, Michel Le Baccon, Veronique Patinec
    Abstract:

    Abstract The topic of this paper is the impregnation with tetraazamacrocyclic ligands of a Lignocellulosic Substrate extracted from wheat bran. A preliminary N-functionalization by an alkyl pendant arm makes the cyclic tetraamine ligands lipophilic, so as to ensure their immobilization on the Substrate. The macrocyclic ligands thus fixed at the surface of the Lignocellulosic Substrate interact with copper ions in solution to form complexes, as proven by EPR spectra. The end result is an increased retention capacity of the Lignocellulosic Substrate for pH values lower than 4. Indeed, while the unmodified Lignocellulosic Substrate does not retain Cu(II) at pH 3, the impregnated Substrate exhibits a retention capacity of 4.13 mg g −1 . As a result, this technique of impregnation seems to be a good solution to extend the application field of Lignocellulosic Substrates, and consider their use in the treatment of acidic effluents.

  • Biosorption of Cu(II) and Zn(II) onto a Lignocellulosic Substrate extracted from wheat bran
    Environmental Chemistry Letters, 2005
    Co-Authors: Laurent Dupont, Jamila Bouanda, Jeanmarc Dumonceau, Michel Aplincourt
    Abstract:

    We studied the removal of copper and zinc ions from aqueous solutions using a Lignocellulosic Substrate obtained by an acido-basic treatment of wheat bran. The sorption capacity of this material was investigated through batch and column experiments. Batch experimental results showed that the retention capacity of the Lignocellulosic Substrate was 0.20×10^−3 mol g^−1 at pH 4.5 for copper(II) and 0.24×10^−3 mol g^−1 at pH 6.5 for zinc(II). Column experiments showed a reduced sorption capacity for both ions compared to batch experiments. Batch and column data were analysed using the Langmuir equation in order to determine the affinity constant and the binding capacity of the sorbent and to compare both retention processes.

  • metal ions binding onto a Lignocellulosic Substrate extracted from wheat bran a nica donnan approach
    Joint International Conference on Information Sciences, 2003
    Co-Authors: Laurent Dupont, Jamila Bouanda, Jeanmarc Dumonceau, Michel Aplincourt
    Abstract:

    In this study ion binding to solid organic matter was investigated. We used the NICA–Donnan model to describe the interaction between Cu(II), Pb(II), and Cd(II) ions and a Lignocellulosic Substrate extracted from wheat bran. Such a material represents a very cheap and flexible Substrate, which can be used as a natural filter to remove toxic metal ions from industrial effluents or applied in the decontamination and rehabilitation of abandoned industrial sites. Moreover, such a material also represents a very simple model of natural organic matter derived from lignin and cellulose, for which there is now a lack of thermodynamic data as far as their interaction with toxic metal ions is concerned. Experimental results were obtained in various conditions of pH and ionic strength. In our modeling, we used the acidity constants and the concentration of sites that were determined in a previous work. The parameters deduced from the NICA–Donnan model were also compared to thermodynamic data available for other cases of natural organic matter, mainly humic substances.

  • use of a nica donnan approach for analysis of proton binding to a Lignocellulosic Substrate extracted from wheat bran
    Analytical and Bioanalytical Chemistry, 2002
    Co-Authors: Jamila Bouanda, Laurent Dupont, Jeanmarc Dumonceau, Michel Aplincourt
    Abstract:

    The acid–base properties of a Lignocellulosic Substrate extracted from wheat bran have been investigated. The Lignocellulosic Substrate was first studied by use of FTIR, XPS, and solid-state 13C NMR to characterize the surface-active groups. Major contributions arise from the presence of carboxylic and phenolic sites. The former are associated with long-chain fatty acids and the latter are constituent units of lignin. All ionizable sites were quantified by use of the Ca-acetate method and by potentiometric titrations in non-aqueous media. Results were compared with those from conductimetric titrations in water and in the presence of barium ions. Protometric titration curves for the Lignocellulosic Substrate were obtained at several ionic strengths. Data were also treated with the NICA–Donnan model to determine the intrinsic ionization parameters.