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J P Boon - One of the best experts on this subject based on the ideXlab platform.
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temporal induction pattern of hepatic cytochrome p450 1a in thermally acclimated dab Limanda Limanda treated with 3 3 4 4 tetrachlorobiphenyl cb77
Chemosphere, 1996Co-Authors: H M Sleiderink, J P BoonAbstract:Mature male dab (Limanda Limanda) acclimated at 10° and 16°C were orally administered a single dose of 0.5 mg/kg 3,3′,4,4′-tetrachlorobiphenyl (CB77). At both temperatures, levels of cytochrome P450 1A (CYP1A) protein and 7-ethoxyresorufin O-deethylase (EROD) activity showed a two to six fold induction 40 days after CB77 treatment compared to control groups. Maximum responses of both EROD activity and CYP1A protein for the warm-acclimated fish were observed at 5 days after treatment. For the cold-acclimated fish a slow, progressive elevation for both EROD activity and CYP1A protein was observed and maximum responses were measured 40 days after treatment. Absolute EROD activity and CYPIA protein levels of fish from both temperatures were equally high at 40 days after treatment. Since in the control groups EROD activity and CYP1A protein levels were higher in the cold-acclimated fish, the magnitude of induction was higher in the warm acclimated ones. The highest concentrations of CB77 in muscle of fish from both temperatures were found at 5 and 10 days after treatment. The liver somatic index (LSI) showed 1.5 fold significantly higher values for the fish acclimated at 10°C.
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cytochrome p450 1a response in north sea dab Limanda Limanda from offshore and coastal sites
Marine Pollution Bulletin, 1995Co-Authors: H M Sleiderink, J P BoonAbstract:Abstract Dab ( Limanda Limanda ) were collected at four stations in the southern North Sea in November 1993. Highest muscle polychlorinated biphenyl (PCB) concentrations were found at a station near the Dutch coast. CB153 was the dominant congener at all stations. Since the concentrations of two mono- ortho Cl-substituted congeners covaried strongly with CB153, this congener appears to be a good marker for general differences in PCB concentrations. Mean CB153 concentrations ranged from 50–160 ng g −1 lipid. The highest cytochrome P450 1A (CYP1A) levels were also found at the coastal station. The extent of induction, measured catalytically (7-ethoxyresorufin O -deethylase, EROD) and immunochemically (CYP1A ELISA), between the less contaminated offshore sites and the coastal site ranged up to five-fold. Both parameters were well correlated with muscle tissue PCB concentrations. The data indicate that the CYP1A induction response in dab populations along the Dutch coast is strong enough to separate the Dutch coastal area from more pristine offshore areas of the North Sea. Compared to results of previous studies in other seasons the present sampling period offered the best conditions to investigate the correlation between environmental contamination with PCBs and related compounds, since the variations in natural factors that also interfere, such as water temperature and condition of the fish, were very low.
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sensitivity of cytochrome p450 1a induction in dab Limanda Limanda of different age and sex as a biomarker for environmental contaminants in the southern north sea
Archives of Environmental Contamination and Toxicology, 1995Co-Authors: H M Sleiderink, I Oostingh, Anders Goksoyr, J P BoonAbstract:The sensitivity of cytochrome P450 1A (CYP1A) induction as a biomarker for environmental contaminants in the flatfish dab (Limanda Limanda) was evaluated by studying fish of different age and sex from the southern North Sea. Mature and juvenile dab from both sexes were collected in autumn and winter during two surveys from four different stations with varying levels of polychlorinated biphenyls (PCBs) contamination in the southern North Sea.
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influence of temperature on cytochrome p450 1a in dab Limanda Limanda from the southern north sea results from field surveys and a laboratory study
Marine Environmental Research, 1995Co-Authors: H M Sleiderink, Jonny Beyer, Jan M Everaarts, J P BoonAbstract:Abstract Mature specimens of male dab were collected from several stations in the southern North Sea, and the cytochrome P450 1A dependent 7-ethoxyresorufin O-deethylase (EROD) activity was measured with a microplate reader directly onboard the research vessel. During both surveys the highest levels of EROD activity were found at relatively clean off-shore stations with low bottom water temperatures due to stratification. Considerably lower EROD values were found along the more contaminated Dutch coast at stations with relatively high bottom water temperatures. The inverse relationship between water temperature and EROD activity was confirmed in a laboratory study, where dabs were acclimated to 8, 12 and 16 °C during a period of four weeks.
Thierry Burgeot - One of the best experts on this subject based on the ideXlab platform.
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Integrated monitoring of chemicals and their effects on four sentinel species, Limanda Limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring
Marine Environmental Research, 2017Co-Authors: Thierry Burgeot, Farida Akcha, Dominique Menard, Craig Robinson, Véronique Loizeau, Christophe Brach-papa, Concepción Martínez-gòmez, Jérémie Le Goff, Hélène Budzinski, Karine Le MénachAbstract:The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine sentinel species (Platichthys flesus, Limanda Limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four species in 2008–2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
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DNA damage in dab (Limanda Limanda) and haddock (Melanogrammus aeglefinus) from European seas
Marine Environmental Research, 2017Co-Authors: Ketil Hylland, Jérémie Le Goff, Bjørn Borge Skei, Gunnar Brunborg, Thomas Lang, Matthew Gubbins, Thierry BurgeotAbstract:Dab (Limanda Limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and individuals for haddock, particularly from Iceland. Adduct concentrations were elevated in haddock from both Iceland and the Firth of Forth. A general linear model (GLM) suggested that, in addition to location, the size of dab and its general condition contributed to explaining the observed variability in DNA strand breaks. A GLM for adducts in dab similarly allocated most of the variability to location, but with a possible contribution from CYP1A activity. There were no apparent differences between male and female dab for any of the methods. There was no obvious relationship between strand breaks and adducts in the same fish although dab from Ekofisk and Iceland had respectively high and low responses using both methods. The results from this large-scale study showed pollution-related genotoxicity for dab, that fish blood samples can be conserved prior to comet analyses and that there are clear species differences in genotoxic responses even when collected at the same location.
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PAH metabolites in fish bile: From the Seine estuary to Iceland
Marine Environmental Research, 2017Co-Authors: U. Kammann, Thierry Burgeot, Hélène Budzinski, Karine Le Ménach, Matthew Gubbins, F Akcha, T. Lang, A.d. Vethaak, Ketil HyllandAbstract:Polycyclic aromatic hydrocarbons (PAH) are environmental contaminants that pose significant risk to health of fish. The International Workshop on Integrated Assessment of Contaminant Impacts on the North Sea (ICON) provided the framework to investigate biomarker responses as well as contaminant concentrations side by side in marine ecosystems. Concentrations of the main PAH metabolites 1-hydroxypyrene, 1-hydroxyphenanthren and 3-hydroxybenzo(a)pyrene were determined in bile by HPLC with fluorescence detection. Fish species under investigation were dab (Limanda Limanda), flounder (Platichthys flesus) and haddock (Melanogrammus aeglefinus). A contamination gradient was demonstrated from the low contaminated waters of Iceland and off-shore regions of the North Sea towards higher concentrations in coastal areas. Concentrations of PAH metabolites differed primarily according to sampling region and secondarily to species.
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monitoring biological effects of contamination in marine fish along french coasts by measurement of ethoxyresorufin o deethylase activity
Ecotoxicology and Environmental Safety, 1994Co-Authors: Thierry Burgeot, Gilles Bocquene, G Pingray, D Godefroy, J Legrand, J Dimeet, F Marco, F Vincent, Y Henocque, Oger H JeanneretAbstract:Abstract The use of bioindicators to evaluate exposure to the biological effects of chemical pollutants in marine organisms constitutes a new tool in the monitoring field. The establishment of a North Sea monitoring network in 1991, involving such international organizations as the North Sea Task Force, the International Council for the Exploration of the Sea, and the Intergovernmental Oceanography Commission, led French researchers to develop an enzymatic biomarker to monitor biological effects within the National Observation Network. The biomarker, ethoxyresorufin-O-deethylase (EROD), dependent on the CP450 system, has been monitored biannually since 1992 in several species of fish (Callionymus lyra, Limanda Limanda, Serranus sp., Mullus barbatus) in two coastal sites particularly exposed to industrial and domestic pollution. A rapid method is used to assay EROD enzymatic activity determined along a pollution gradient, and results are interpreted on a microplate reader. The strategy of this approach is to assess the effects on the marine ecosystem during prolonged exposure to specific pollutants such as polyaromatic hydrocarbons, polychlorinated biphenyls, and dioxins.
H M Sleiderink - One of the best experts on this subject based on the ideXlab platform.
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temporal induction pattern of hepatic cytochrome p450 1a in thermally acclimated dab Limanda Limanda treated with 3 3 4 4 tetrachlorobiphenyl cb77
Chemosphere, 1996Co-Authors: H M Sleiderink, J P BoonAbstract:Mature male dab (Limanda Limanda) acclimated at 10° and 16°C were orally administered a single dose of 0.5 mg/kg 3,3′,4,4′-tetrachlorobiphenyl (CB77). At both temperatures, levels of cytochrome P450 1A (CYP1A) protein and 7-ethoxyresorufin O-deethylase (EROD) activity showed a two to six fold induction 40 days after CB77 treatment compared to control groups. Maximum responses of both EROD activity and CYP1A protein for the warm-acclimated fish were observed at 5 days after treatment. For the cold-acclimated fish a slow, progressive elevation for both EROD activity and CYP1A protein was observed and maximum responses were measured 40 days after treatment. Absolute EROD activity and CYPIA protein levels of fish from both temperatures were equally high at 40 days after treatment. Since in the control groups EROD activity and CYP1A protein levels were higher in the cold-acclimated fish, the magnitude of induction was higher in the warm acclimated ones. The highest concentrations of CB77 in muscle of fish from both temperatures were found at 5 and 10 days after treatment. The liver somatic index (LSI) showed 1.5 fold significantly higher values for the fish acclimated at 10°C.
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cytochrome p450 1a response in north sea dab Limanda Limanda from offshore and coastal sites
Marine Pollution Bulletin, 1995Co-Authors: H M Sleiderink, J P BoonAbstract:Abstract Dab ( Limanda Limanda ) were collected at four stations in the southern North Sea in November 1993. Highest muscle polychlorinated biphenyl (PCB) concentrations were found at a station near the Dutch coast. CB153 was the dominant congener at all stations. Since the concentrations of two mono- ortho Cl-substituted congeners covaried strongly with CB153, this congener appears to be a good marker for general differences in PCB concentrations. Mean CB153 concentrations ranged from 50–160 ng g −1 lipid. The highest cytochrome P450 1A (CYP1A) levels were also found at the coastal station. The extent of induction, measured catalytically (7-ethoxyresorufin O -deethylase, EROD) and immunochemically (CYP1A ELISA), between the less contaminated offshore sites and the coastal site ranged up to five-fold. Both parameters were well correlated with muscle tissue PCB concentrations. The data indicate that the CYP1A induction response in dab populations along the Dutch coast is strong enough to separate the Dutch coastal area from more pristine offshore areas of the North Sea. Compared to results of previous studies in other seasons the present sampling period offered the best conditions to investigate the correlation between environmental contamination with PCBs and related compounds, since the variations in natural factors that also interfere, such as water temperature and condition of the fish, were very low.
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sensitivity of cytochrome p450 1a induction in dab Limanda Limanda of different age and sex as a biomarker for environmental contaminants in the southern north sea
Archives of Environmental Contamination and Toxicology, 1995Co-Authors: H M Sleiderink, I Oostingh, Anders Goksoyr, J P BoonAbstract:The sensitivity of cytochrome P450 1A (CYP1A) induction as a biomarker for environmental contaminants in the flatfish dab (Limanda Limanda) was evaluated by studying fish of different age and sex from the southern North Sea. Mature and juvenile dab from both sexes were collected in autumn and winter during two surveys from four different stations with varying levels of polychlorinated biphenyls (PCBs) contamination in the southern North Sea.
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influence of temperature on cytochrome p450 1a in dab Limanda Limanda from the southern north sea results from field surveys and a laboratory study
Marine Environmental Research, 1995Co-Authors: H M Sleiderink, Jonny Beyer, Jan M Everaarts, J P BoonAbstract:Abstract Mature specimens of male dab were collected from several stations in the southern North Sea, and the cytochrome P450 1A dependent 7-ethoxyresorufin O-deethylase (EROD) activity was measured with a microplate reader directly onboard the research vessel. During both surveys the highest levels of EROD activity were found at relatively clean off-shore stations with low bottom water temperatures due to stratification. Considerably lower EROD values were found along the more contaminated Dutch coast at stations with relatively high bottom water temperatures. The inverse relationship between water temperature and EROD activity was confirmed in a laboratory study, where dabs were acclimated to 8, 12 and 16 °C during a period of four weeks.
Karine Le Ménach - One of the best experts on this subject based on the ideXlab platform.
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Integrated monitoring of chemicals and their effects on four sentinel species, Limanda Limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring
Marine Environmental Research, 2017Co-Authors: Thierry Burgeot, Farida Akcha, Dominique Menard, Craig Robinson, Véronique Loizeau, Christophe Brach-papa, Concepción Martínez-gòmez, Jérémie Le Goff, Hélène Budzinski, Karine Le MénachAbstract:The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine sentinel species (Platichthys flesus, Limanda Limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four species in 2008–2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
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PAH metabolites in fish bile: From the Seine estuary to Iceland
Marine Environmental Research, 2017Co-Authors: U. Kammann, Thierry Burgeot, Hélène Budzinski, Karine Le Ménach, Matthew Gubbins, F Akcha, T. Lang, A.d. Vethaak, Ketil HyllandAbstract:Polycyclic aromatic hydrocarbons (PAH) are environmental contaminants that pose significant risk to health of fish. The International Workshop on Integrated Assessment of Contaminant Impacts on the North Sea (ICON) provided the framework to investigate biomarker responses as well as contaminant concentrations side by side in marine ecosystems. Concentrations of the main PAH metabolites 1-hydroxypyrene, 1-hydroxyphenanthren and 3-hydroxybenzo(a)pyrene were determined in bile by HPLC with fluorescence detection. Fish species under investigation were dab (Limanda Limanda), flounder (Platichthys flesus) and haddock (Melanogrammus aeglefinus). A contamination gradient was demonstrated from the low contaminated waters of Iceland and off-shore regions of the North Sea towards higher concentrations in coastal areas. Concentrations of PAH metabolites differed primarily according to sampling region and secondarily to species.
Hilmar Hinz - One of the best experts on this subject based on the ideXlab platform.
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Effects of bottom trawling on fish foraging and feeding.
Proceedings of the Royal Society B: Biological Sciences, 2015Co-Authors: Andrew F. Johnson, Jan G. Hiddink, Giulia Gorelli, Stuart R. Jenkins, Hilmar HinzAbstract:The effects of bottom trawling on benthic invertebrates include reductions of biomass, diversity and body size. These changes may negatively affect prey availability for demersal fishes, potentially leading to reduced food intake, body condition and yield of fishes in chronically trawled areas. Here, the effect of trawling on the prey availability and diet of two commercially important flatfish species, plaice (Pleuronectes platessa) and dab (Limanda Limanda), was investigated over a trawling intensity gradient in the Irish Sea. Previous work in this area has shown that trawling negatively affects the condition of plaice but not of dab. This study showed that reductions in local prey availability did not result in reduced feeding of fish. As trawling frequency increased, both fish and prey biomass declined, such that the ratio of fish to prey remained unchanged. Consequently, even at frequently trawled sites with low prey biomass, both plaice and dab maintained constant levels of stomach fullness and gut energy contents. However, dietary shifts in plaice towards energy-poor prey items were evident when prey species were analysed individually. This, together with a potential decrease in foraging efficiency due to low prey densities, was seen as the most plausible cause for the reduced body condition observed. Understanding the relationship between trawling, benthic impacts, fish foraging and resultant body condition is an important step in designing successful mitigation measures for future management strategies in bottom trawl fisheries.
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the feeding strategy of dab Limanda Limanda in the southern north sea linking stomach contents to prey availability in the environment
Journal of Fish Biology, 2005Co-Authors: Hilmar Hinz, Ingrid Kröncke, Siegfried EhrichAbstract:The feeding strategy of dab Limanda Limanda was investigated within a predefined research area in the southern North Sea for 2 years. The aims were: 1) to establish the relationship between prey availability and dab abundance, distribution, body condition and prey selection, and 2) to determine the underlying functional processes of prey choice in dab. Temporal and spatial trends of prey availability in relation to dab abundances, distribution, condition and prey selection were analysed. Temporal trends in dab condition, percentage of empty stomachs and numbers of ingested prey were apparent. In particular in the winter period 2000-2001 dab were in poor condition and prey items were only rarely found in stomachs. Similarly prey availability decreased in the environment while the abundance of dab increased. Thus, temporal changes in the nutritive state were partly thought to be attributed to density-dependent effects. The stomach composition of dab during the two consecutive summers was mainly dominated by Pariambus typicus and Ophiura albida while in the winter period only O. albida made a significant contribution to the stomach content. Analysis of prey characteristics indicated that only prey densities in the environment significantly influenced prey choice in dab, while other characteristics such as the position of the prey in the habitat, its palatability or mobility did not have significant effects. The feeding strategy of dab thus was highly opportunistic, although trends were apparent that suggested that buried living fauna was less likely to be ingested, which was also observed in previous studies.
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Demersal fish and epifauna associated with sandbank habitats
Estuarine Coastal and Shelf Science, 2004Co-Authors: Michel J. Kaiser, Hilmar Hinz, Melanie Bergmann, Maria Galanidi, Richard Shucksmith, Edward Rees, Teresa Darbyshire, K. RamsayAbstract:A habitat specific survey of the epifauna and fish fauna of sandbanks off the Welsh coastline was undertaken in 2001. Of these, three sandbanks were considered to represent extensions of shallow nearshore soft-sediment communities, while a further six sandbanks were considered to be distinct sandbanks; seabed features clearly defined in comparison with surrounding sediments. Multivariate community analyses revealed that the distinct sandbanks had both fish and epifaunal assemblages that were distinct from those sandbanks considered to be extensions of nearshore sediments. The distinct sandbanks were typified by low species diversity and shared indicator species such as the weever fish Echiichthys vipera, the shrimp Philocheras trispinosus and the hermit crab Pagurus bernhardus. Differences occurred in species composition among the distinct sandbanks, in particular, southern sandbanks were typified by sand sole Solea lascaris and small-eyed ray Raja microocellata. The sandbanks considered as extensions of nearshore sediments shared many similarities with the Pleuronectes platessa–Limanda Limanda assemblage, identified by Ellis et al. (Estuar. Coastal Shelf Sci. 51 (2000) 299), which is widespread in the Irish Sea. Sandbanks, as a habitat definition under the EU habitats directive, are likely to incorporate a number of physically and biologically distinct habitats of which two have been described in the present study. 2004 Elsevier Ltd. All rights reserved.
