The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Shijia Liu - One of the best experts on this subject based on the ideXlab platform.
-
Limonin ameliorates ulcerative colitis by regulating STAT3/miR-214 signaling pathway.
International immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong ZhouAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
-
Limonin ameliorates ulcerative colitis by regulating stat3 mir 214 signaling pathway
International Immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong Zhou, Yugen ChenAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
-
biotransformation and metabolic profile of Limonin in rat liver microsomes bile and urine by high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Shijia Liu, Guoliang Dai, Luning Sun, Bingting Sun, Du Chen, Lei Zhu, Yao Wang, Li Zhang, Peidong Chen, Dong ZhouAbstract:Limonin is a triterpenoid in citrus seeds, which has significant biological activities. However, the metabolic profile of Limonin has not been fully understood. To expound its metabolism in vivo and in vitro, the metabolites of Limonin was studied by rat liver microsomes, urine, and bile. High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used for identification. Among the metabolites, the structures of M1 and M3 were confirmed by chemical synthesis and nuclear magnetic resonance spectra analysis. Our results indicated that reduction and hydrolysis were the two major pathways during Limonin metabolism in vivo and in vitro. The results from this work are valuable and important for understanding the metabolic process of Limonin.
-
Biotransformation and Metabolic Profile of Limonin in Rat Liver Microsomes, Bile, and Urine by High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
2018Co-Authors: Shijia Liu, Guoliang Dai, Luning Sun, Bingting Sun, Du Chen, Lei Zhu, Yao Wang, Li Zhang, Peidong Chen, Dong ZhouAbstract:Limonin is a triterpenoid in citrus seeds, which has significant biological activities. However, the metabolic profile of Limonin has not been fully understood. To expound its metabolism in vivo and in vitro, the metabolites of Limonin was studied by rat liver microsomes, urine, and bile. High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used for identification. Among the metabolites, the structures of M1 and M3 were confirmed by chemical synthesis and nuclear magnetic resonance spectra analysis. Our results indicated that reduction and hydrolysis were the two major pathways during Limonin metabolism in vivo and in vitro. The results from this work are valuable and important for understanding the metabolic process of Limonin
-
Comprehensive characterization of the in vitro and in vivo metabolites of Limonin in human samples using LC-Q-TOF/MS.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2017Co-Authors: Shijia Liu, Guoliang Dai, Luning Sun, Lei Zhu, Peidong Chen, Nong-shan Zhang, Yang Zhao, Li ZhangAbstract:Abstract Limonin is a bitter triterpenoid dilactone in the genus Citrus with potential medicinal value. In this study, the metabolism of Limonin in human was characterized by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). A total of 7 metabolites were identified from human samples. Among them, 3 metabolites of M1, M2 and M4 were detected in urine and feces, and the others were found in intestinal bacteria sample. Notably, M1 and M3 were chemically synthesized, of which the structures were further confirmed by NMR spectra data. The metabolism of Limonin involved three major pathways, namely, reduction, hydrolysis and methylation. The reduction and hydrolysis were commonly observed in ring D of Limonin. The metabolites showed decomposition in ring A. This study provides data for the metabolism of Limonin in humans, and will contribute to explain its biological activity.
Dong Zhou - One of the best experts on this subject based on the ideXlab platform.
-
Limonin ameliorates ulcerative colitis by regulating STAT3/miR-214 signaling pathway.
International immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong ZhouAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
-
Limonin ameliorates ulcerative colitis by regulating stat3 mir 214 signaling pathway
International Immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong Zhou, Yugen ChenAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
-
biotransformation and metabolic profile of Limonin in rat liver microsomes bile and urine by high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Shijia Liu, Guoliang Dai, Luning Sun, Bingting Sun, Du Chen, Lei Zhu, Yao Wang, Li Zhang, Peidong Chen, Dong ZhouAbstract:Limonin is a triterpenoid in citrus seeds, which has significant biological activities. However, the metabolic profile of Limonin has not been fully understood. To expound its metabolism in vivo and in vitro, the metabolites of Limonin was studied by rat liver microsomes, urine, and bile. High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used for identification. Among the metabolites, the structures of M1 and M3 were confirmed by chemical synthesis and nuclear magnetic resonance spectra analysis. Our results indicated that reduction and hydrolysis were the two major pathways during Limonin metabolism in vivo and in vitro. The results from this work are valuable and important for understanding the metabolic process of Limonin.
-
Biotransformation and Metabolic Profile of Limonin in Rat Liver Microsomes, Bile, and Urine by High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
2018Co-Authors: Shijia Liu, Guoliang Dai, Luning Sun, Bingting Sun, Du Chen, Lei Zhu, Yao Wang, Li Zhang, Peidong Chen, Dong ZhouAbstract:Limonin is a triterpenoid in citrus seeds, which has significant biological activities. However, the metabolic profile of Limonin has not been fully understood. To expound its metabolism in vivo and in vitro, the metabolites of Limonin was studied by rat liver microsomes, urine, and bile. High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used for identification. Among the metabolites, the structures of M1 and M3 were confirmed by chemical synthesis and nuclear magnetic resonance spectra analysis. Our results indicated that reduction and hydrolysis were the two major pathways during Limonin metabolism in vivo and in vitro. The results from this work are valuable and important for understanding the metabolic process of Limonin
Yugen Chen - One of the best experts on this subject based on the ideXlab platform.
-
Limonin ameliorates ulcerative colitis by regulating stat3 mir 214 signaling pathway
International Immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong Zhou, Yugen ChenAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
Nittaya Kasemsuksakul - One of the best experts on this subject based on the ideXlab platform.
-
Effect of maturity and processing on bitter compounds in Thai tangerine juice
International Journal of Food Science and Technology, 2007Co-Authors: Athapol Noomhorm, Nittaya KasemsuksakulAbstract:Summary Optimum conditions of fruit maturity and processing for improved quality of Thai tangerine fruit juice were evaluated. Limonin and naringin components causing bitterness, acidity, total soluble solids and vitamin C were quantified in specified fruit setting and processing conditions. Higher Limonin contents were observed in tangerine fruits harvested early in the season of 1989, whereas naringin contents gradually decreased with maturity. The optimum harvesting time for Thai tangerine fruit which meets the worldwide quality indicators of extracted juice was nine months after fruit set. Low temperature storage of tangerine juice was only effective in delaying Limonin formation if not pasteurized, which resulted in higher Limonin concentrations at the start of the storage period. However, naringin concentrations of tangerine juice were not affected by storage conditions and the pasteurization process. Lower extraction pressure of juice resulted in low Limonin and naringin concentrations.
Cong Ren - One of the best experts on this subject based on the ideXlab platform.
-
Limonin ameliorates ulcerative colitis by regulating STAT3/miR-214 signaling pathway.
International immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong ZhouAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
-
Limonin ameliorates ulcerative colitis by regulating stat3 mir 214 signaling pathway
International Immunopharmacology, 2019Co-Authors: Shijia Liu, Shufang Zhang, Cong Ren, Zhiqin Zeng, Lufeng Zheng, Xianke Zhou, Dong Zhou, Yugen ChenAbstract:Abstract Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of Limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of Limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that Limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, Limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that Limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that Limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.