The Experts below are selected from a list of 29838 Experts worldwide ranked by ideXlab platform
Jun Chen - One of the best experts on this subject based on the ideXlab platform.
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identification and analysis of trna genes provide new insights into oil Biosynthesis in tung tree vernicia fordii hemsl
Industrial Crops and Products, 2019Co-Authors: Yanru Fan, Jun Chen, Wenjuan Liu, Xu Zhou, Xinwei Tang, Lin ZhangAbstract:Abstract Tung tree (Vernicia fordii Hemsl.) is a potential commercial source of biodiesel which is widely planted in China and many other countries. Investigation of the regulatory mechanism of Lipid Biosynthesis is an essential prerequisite to improve tung tree varieties by genetic engineering. In this study, the transfer RNA (tRNA) genes were identified in four genome-sequenced species (tung tree, rubber tree, castor bean and physic nut) in Euphorbiaceae. Gene clusters were observed for the four species tRNAs. Though tRNAs were highly conserved, contrasting patterns of nucleotide diversity were observed among the tRNA family. A total of 86 differentially expressed tRNA-derived RNA fragments (tRFs) were identified of which the target genes were enriched in Lipid Biosynthesis by Gene Ontology (GO) enrichment analysis. Interestingly, 11 Lipid genes previously reported were identified and found to be regulated by 17 tRFs. A tRF-mRNA regulatory network was constructed, which provides a new insight into the genetic mechanism of Lipid Biosynthesis in tung tree.
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New insight into LncRNA-mRNA regulatory network associated with Lipid Biosynthesis using Hi-C data in seeds of tung tree (Vernicia fordii Hemsl.)
Industrial Crops and Products, 2026Co-Authors: Jun Chen, Dejuan Huang, Wen Zhai, Chuanhong ChenAbstract:Abstract Tung tree (Vernicia fordii Hemsl.), widely distributed in China, is a major potential source of biofuel. Exploration of the Lipid Biosynthesis regulatory network is essential for increasing tung tree Lipid yield and its economic value. LncRNAs play significant regulatory roles in many different biological processes in plants. In this study, the profile and differential expression of lncRNAs and mRNAs were evaluated in tung tree seeds in two Lipid accumulation phases using high-throughput sequencing technologies. GO function analysis of the protein-coding genes revealed that the majority of up-regulated genes were enriched in the categories of carbohydrate transport (GO:0008643), positive regulation of seed germination (GO:0010030), seed oilbody biogenesis (GO:0010344) and Lipid metabolic process (GO:0006629). Network analysis showed that 177 up-regulated lncRNAs might regulate 1290 target genes. GO function analysis was performed on the lncRNA up-regulated target genes, revealed that the Lipid particle organization (GO:0034389) was enriched in the biological process. Associated chromatin interactions between lncRNAs and mRNAs were conducted according to Hi-C data. A total of 68 lncRNAs and 46 genes were included in the Lipid Biosynthesis regulatory network, which provides useful information to reveal the interaction between lncRNAs and coding genes related to Lipid Biosynthesis in tung tree.
Xu Ya Yu - One of the best experts on this subject based on the ideXlab platform.
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improvement in Lipid production in monoraphidium sp qly 1 by combining fulvic acid treatment and salinity stress
Bioresource Technology, 2019Co-Authors: Ximing Li, Yong Teng Zhao, Xingyu Li, Peng Zhao, Xu Ya YuAbstract:Abstract The effects of the combined treatment of fulvic acid (FA) and salinity stress on Lipid production in Monoraphidium sp. QLY-1 at multiple levels was investigated in this study. The results indicated that the highest Lipid content (59.53%) in QLY-1 was achieved by combining FA treatment and salinity stress. Compared with the control group and FA addition alone, the group treated with both FA and salinity stress had increased contents of reactive oxygen species (ROS), antioxidases, and nitric oxide (NO). Additionally, the addition of FA enhanced the expression levels of mitogen-activated protein kinases (MAPKs) and key genes related to Lipid Biosynthesis in QLY-1 under salinity stress. Collectively, biochemical analyses indicated that ROS, NO, MAPK, expression of Lipid Biosynthesis-related genes and antioxidant systems were involved in the Lipid Biosynthesis pathways of QLY-1 under the combined treatment of FA and salinity stress.
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enhancement of Lipid accumulation in monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Junwei Xu, Peng Zhao, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, Lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The Lipid content and Lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and Lipid Biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and Lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and Lipid Biosynthesis by modulating the levels of NO and Lipid Biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of Lipid Biosynthesis in microalgae under SL treatment.
John L Harwood - One of the best experts on this subject based on the ideXlab platform.
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studies on the regulation of Lipid Biosynthesis in plants application of control analysis to soybean
Biochimica et Biophysica Acta, 2014Co-Authors: Irina A. Guschina, Patti A. Quant, John D Everard, Anthony J Kinney, John L HarwoodAbstract:Although there is much knowledge of the enzymology (and genes coding the proteins) of Lipid Biosynthesis in higher plants, relatively little attention has been paid to regulation. We have demonstrated the important role for cholinephosphate cytidylyltransferase in the Biosynthesis of the major extra-plastidic membrane Lipid, phosphatidylcholine. We followed this work by applying control analysis to light-induced fatty acid synthesis. This was the first such application to Lipid synthesis in any organism. The data showed that acetyl-CoA carboxylase was very important, exerting about half of the total control. We then applied metabolic control analysis to Lipid accumulation in important oil crops - oilpalm, olive, and rapeseed. Recent data with soybean show that the block of fatty acid Biosynthesis reactions exerts somewhat more control (63%) than Lipid assembly although both are clearly very important. These results suggest that gene stacks, targeting both parts of the overall Lipid synthesis pathway will be needed to increase significantly oil yields in soybean. This article is part of a Special Issue entitled: Membrane Structure and Function: Relevance in the Cell's Physiology, Pathology and Therapy.
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Use of metabolic control analysis to give quantitative information on control of Lipid Biosynthesis in the important oil crop, Elaeis guineensis (oilpalm)
The New phytologist, 2009Co-Authors: Umi S Ramli, Patti A. Quant, Joaquín J. Salas, John L HarwoodAbstract:* Oil crops are a very important commodity. Although many genes and enzymes involved in Lipid accumulation have been identified, much less is known of regulation of the overall process. To address the latter we have applied metabolic control analysis to Lipid synthesis in the important crop, oilpalm (Elaeis guineensis). * Top-down metabolic control analysis (TDCA) was applied to callus cultures capable of accumulating appreciable triacylglycerol. The biosynthetic pathway was divided into two blocks, connected by the intermediate acyl-CoAs. Block A comprised enzymes for fatty acid synthesis and Block B comprised enzymes of Lipid assembly. * Double manipulation TDCA used diflufenican and bromooctanoate to inhibit Block A and Block B, respectively, giving Block flux control coefficients of 0.61 and 0.39. Monte Carlo simulations provided extra information from previously-reported single manipulation TDCA data, giving Block flux control coefficients of 0.65 and 0.35 for A and B. * These experiments are the first time that double manipulation TDCA has been applied to Lipid Biosynthesis in any organism. The data show that approaching two-thirds of the total control of carbon flux to Lipids in oilpalm cultures lies with the fatty acid synthesis block of reactions. This quantitative information will assist future, informed, genetic manipulation of oilpalm.
Harikrishna Kulaveerasingam - One of the best experts on this subject based on the ideXlab platform.
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differential abundance analysis of mesocarp protein from high and low yielding oil palms associates non oil biosynthetic enzymes to Lipid Biosynthesis
Proteome Science, 2015Co-Authors: Tony Eng Keong Ooi, Fook Tim Chew, David Ross Appleton, Wan Chin Yeap, Leona Daniela Jeffery Daim, Fong Chin Lee, Ainul Masni Bt Othman, Harikrishna KulaveerasingamAbstract:The oil palm Elaeis guineensis Jacq. which produces the highest yield per unit land area of the oil crops is the most important commercial oil crop in South East Asia. The fleshy mesocarp of oil palm fruit, where oil is mostly derived from, contains up to 90 % dry weight of oil (one of the most concentrated in plant tissues). Hence, there is attention given to gain insights into the processes of oil deposition in this oil rich tissue. For that purpose, two-dimensional differential gel electrophoresis (DIGE) coupled with western assays, were used here to analyze differential protein levels in genetically-related high-and low-yielding oil palm mesocarps. From the DIGE comparative analysis in combination with western analysis, 41 unique differentially accumulated proteins were discovered. Functional categorization of these proteins placed them in the metabolisms of Lipid, carbohydrate, amino acids, energy, structural proteins, as well as in other functions. In particular, higher abundance of fructose-1,6-biphosphate aldolase combined with reduced level of triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase may be indicative of important flux balance changes in glycolysis, while amino acid metabolism also appeared to be closely linked with oil yield. Forty-one proteins in several important biological pathways were identified as exhibiting differential in abundance at critical oil production stages. These confirm that oil yield is a complex trait involving the regulation of genes in multiple biological pathways. The results also provide insights into key control points of Lipid Biosynthesis in oil palm and can assist in the development of genetic markers for use in oil palm breeding programmes.
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differential metabolite profiles during fruit development in high yielding oil palm mesocarp
PLOS ONE, 2013Co-Authors: Huey Fang Teh, Fook Tim Chew, Bee Keat Neoh, Yin Mee Thang, Mohaimi Mohamed, May Ping Li Hong, Jaime Yoke Sum Low, Nalisha Ithnin, Hirzun Mohd Yusof, Harikrishna KulaveerasingamAbstract:To better understand Lipid Biosynthesis in oil palm mesocarp, in particular the differences in gene regulation leading to and including de novo fatty acid Biosynthesis, a multi-platform metabolomics technology was used to profile mesocarp metabolites during six critical stages of fruit development in comparatively high- and low-yielding oil palm populations. Significantly higher amino acid levels preceding Lipid Biosynthesis and nucleosides during Lipid Biosynthesis were observed in a higher yielding commercial palm population. Levels of metabolites involved in glycolysis revealed interesting divergence of flux towards glycerol-3-phosphate, while carbon utilization differences in the TCA cycle were proven by an increase in malic acid/citric acid ratio. Apart from insights into the regulation of enhanced Lipid production in oil palm, these results provide potentially useful metabolite yield markers and genes of interest for use in breeding programmes.
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profiling of metabolites in oil palm mesocarp at different stages of oil Biosynthesis
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Bee Keat Neoh, Fook Tim Chew, Harikrishna Kulaveerasingam, Huey Fang Teh, Soon Huat Tiong, Yin Mee Thang, Mohd Amiron Ersad, Mohaimi Mohamed, David Ross AppletonAbstract:Oil palm is one of the most productive oil producing crops and can store up to 90% oil in its fruit mesocarp. However, the biosynthetic regulation and drivers of palm mesocarp development are still not well understood. Multiplatform metabolomics technology was used to profile palm metabolites during six critical stages of fruit development in order to better understand Lipid Biosynthesis. Significantly higher amino acid levels were observed in palm mesocarp preceding Lipid Biosynthesis. Nucleosides were found to be in high concentration during Lipid Biosynthesis, whereas levels of metabolites involved in the tricarboxylic acid cycle were more concentrated during early fruit development. Apart from insights into the regulation of metabolites during fruit development in oil palm, these results provide potentially useful metabolite yield markers and genes of interest for use in breeding programs.
Lin Zhang - One of the best experts on this subject based on the ideXlab platform.
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identification and analysis of trna genes provide new insights into oil Biosynthesis in tung tree vernicia fordii hemsl
Industrial Crops and Products, 2019Co-Authors: Yanru Fan, Jun Chen, Wenjuan Liu, Xu Zhou, Xinwei Tang, Lin ZhangAbstract:Abstract Tung tree (Vernicia fordii Hemsl.) is a potential commercial source of biodiesel which is widely planted in China and many other countries. Investigation of the regulatory mechanism of Lipid Biosynthesis is an essential prerequisite to improve tung tree varieties by genetic engineering. In this study, the transfer RNA (tRNA) genes were identified in four genome-sequenced species (tung tree, rubber tree, castor bean and physic nut) in Euphorbiaceae. Gene clusters were observed for the four species tRNAs. Though tRNAs were highly conserved, contrasting patterns of nucleotide diversity were observed among the tRNA family. A total of 86 differentially expressed tRNA-derived RNA fragments (tRFs) were identified of which the target genes were enriched in Lipid Biosynthesis by Gene Ontology (GO) enrichment analysis. Interestingly, 11 Lipid genes previously reported were identified and found to be regulated by 17 tRFs. A tRF-mRNA regulatory network was constructed, which provides a new insight into the genetic mechanism of Lipid Biosynthesis in tung tree.