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Kangsen Mai - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Dietary bile acid (BA) on the growth performance, body composition, antioxidant responses and expression of Lipid metabolism-related genes of juvenile large yellow croaker (Larimichthys crocea) fed high-Lipid Diets
    Aquaculture, 2020
    Co-Authors: Tao Ding, Yongtao Liu, Xiaojun Xiang, Qiangde Liu, Zhaoyang Yin, Kangsen Mai
    Abstract:

    Abstract The present study was conducted to investigate the effects of Dietary bile acid (BA) on the growth performance, body composition, antioxidant responses and expression of Lipid metabolism-related genes of juvenile large yellow croaker (Larimichthys crocea) (average weight, 12.00 ± 0.20 g). An optimal Lipid Diet formulated with 4.0% fish oil and 4.0% soybean oil was set as the positive control Diet (MO), while a high-Lipid Diet formulated with 6.5% fish oil and 6.5% soybean oil was used as the negative control Diet (HO). The other three Diets were supplemented with 150 (HOB150), 300 (HOB300) and 450 mg/kg (HOB450) BA on the basis of the negative control Diet. The results showed that the survival rate (SR) was not significantly different among Dietary treatments (P > 0. 05). Compared with the positive control, there were no significant differences in final body weight (FBW) and weight gain rate (WGR) among fish fed Diets with high Lipid contents. With increasing supplementation of BA in Diets, the FBW and WGR gradually increased. Compared with the negative control, fish fed the Diet with 300 mg/kg BA had significantly higher FBW and WGR (P

  • Characterization of Cyclooxygenase-2 and its induction pathways in response to high Lipid Diet-induced inflammation in Larmichthys crocea
    Scientific reports, 2016
    Co-Authors: Tianjiao Wang, Jing Yan, Kangsen Mai
    Abstract:

    The present study was conducted to investigate the effects of a high-Lipid Diet (HLD) on cyclooxygenase (Cox)-2 expression and the signalling pathways related to low-grade inflammation in the large yellow croaker (Larmichthys crocea). An isolated 2508 bp cDNA clone of cox-2 contained an open reading frame spanning 1827 bp encoding a protein with 608 amino acid residues. The over-expression of cox-2 was consistent with the activation of c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinase (MAPK) in HLD-fed fish. The activation of the activator protein-1 (AP-1) and the nuclear transcription factor kappa-B (NF-κB) signalling pathways in HLD-fed fish and the significant increase of cox-2 promoter-luciferase activity in vitro indicated that AP-1 and NF-κB could combine cox-2 promoter to promote its transcription, respectively. Together, HLD-induced inflammation up-regulates cox-2 expression via JNKs and p38 MAPK-dependent NF-κB and AP-1 pathways. The present study provides important insight into the signal transduction pathways involved in HLD-induced inflammation, which is detrimental to the health and production of fish as well as to the health of fish consumers.

  • Dietary Lipid levels influence Lipid deposition in the liver of large yellow croaker larimichthys crocea by regulating lipoprotein receptors fatty acid uptake and triacylglycerol synthesis and catabolism at the transcriptional level
    PLOS ONE, 2015
    Co-Authors: Jing Yan, Tianjiao Wang, Kai Liao, Kangsen Mai
    Abstract:

    Ectopic Lipid accumulation has been observed in fish fed a high-Lipid Diet. However, no information is available on the mechanism by which Dietary Lipid levels comprehensively regulate Lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how Dietary Lipids affect Lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a Diet with a low (6%), moderate (12%, the control Diet) or high (18%) crude Lipid content for 10 weeks. Growth performance, plasma biochemical indexes, Lipid contents and gene expression related to Lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-Lipid Diet. In the livers of fish fed the high-Lipid Diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control Diet, and hepatic Lipid deposition increased. In fish fed the low-Lipid Diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control Diet. However, hepatic Lipid content in fish fed the low-Lipid Diet decreased mainly due to low Dietary Lipid intake. In summary, findings of this study provide molecular insight into the role of Lipid deposition in the liver in response to different Dietary Lipid contents.

Yizhen Wang - One of the best experts on this subject based on the ideXlab platform.

  • adipose triglyceride lipase atgl clone expression pattern and regulation by different Lipid sources and Lipid levels in large yellow croaker pseudosciaena crocea r
    Marine Biotechnology, 2013
    Co-Authors: Xinxia Wang, Yizhen Wang
    Abstract:

    Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5′UTR, a 1,485-bp open reading frame, and a 194-bp 3′UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of Lipid levels and Lipid sources on ATGL expression in vivo were also investigated. A high-Lipid Diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish Lipid metabolism.

  • Adipose triglyceride lipase (ATGL) clone, expression pattern, and regulation by different Lipid sources and Lipid levels in large yellow croaker (Pseudosciaena crocea R.).
    Marine biotechnology (New York N.Y.), 2012
    Co-Authors: Xinxia Wang, Yizhen Wang
    Abstract:

    Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5′UTR, a 1,485-bp open reading frame, and a 194-bp 3′UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of Lipid levels and Lipid sources on ATGL expression in vivo were also investigated. A high-Lipid Diet decreased ATGL expression in fish significantly (P 

Xinxia Wang - One of the best experts on this subject based on the ideXlab platform.

  • adipose triglyceride lipase atgl clone expression pattern and regulation by different Lipid sources and Lipid levels in large yellow croaker pseudosciaena crocea r
    Marine Biotechnology, 2013
    Co-Authors: Xinxia Wang, Yizhen Wang
    Abstract:

    Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5′UTR, a 1,485-bp open reading frame, and a 194-bp 3′UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of Lipid levels and Lipid sources on ATGL expression in vivo were also investigated. A high-Lipid Diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish Lipid metabolism.

  • Adipose triglyceride lipase (ATGL) clone, expression pattern, and regulation by different Lipid sources and Lipid levels in large yellow croaker (Pseudosciaena crocea R.).
    Marine biotechnology (New York N.Y.), 2012
    Co-Authors: Xinxia Wang, Yizhen Wang
    Abstract:

    Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5′UTR, a 1,485-bp open reading frame, and a 194-bp 3′UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of Lipid levels and Lipid sources on ATGL expression in vivo were also investigated. A high-Lipid Diet decreased ATGL expression in fish significantly (P 

Jing Yan - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of Cyclooxygenase-2 and its induction pathways in response to high Lipid Diet-induced inflammation in Larmichthys crocea
    Scientific reports, 2016
    Co-Authors: Tianjiao Wang, Jing Yan, Kangsen Mai
    Abstract:

    The present study was conducted to investigate the effects of a high-Lipid Diet (HLD) on cyclooxygenase (Cox)-2 expression and the signalling pathways related to low-grade inflammation in the large yellow croaker (Larmichthys crocea). An isolated 2508 bp cDNA clone of cox-2 contained an open reading frame spanning 1827 bp encoding a protein with 608 amino acid residues. The over-expression of cox-2 was consistent with the activation of c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinase (MAPK) in HLD-fed fish. The activation of the activator protein-1 (AP-1) and the nuclear transcription factor kappa-B (NF-κB) signalling pathways in HLD-fed fish and the significant increase of cox-2 promoter-luciferase activity in vitro indicated that AP-1 and NF-κB could combine cox-2 promoter to promote its transcription, respectively. Together, HLD-induced inflammation up-regulates cox-2 expression via JNKs and p38 MAPK-dependent NF-κB and AP-1 pathways. The present study provides important insight into the signal transduction pathways involved in HLD-induced inflammation, which is detrimental to the health and production of fish as well as to the health of fish consumers.

  • Dietary Lipid levels influence Lipid deposition in the liver of large yellow croaker larimichthys crocea by regulating lipoprotein receptors fatty acid uptake and triacylglycerol synthesis and catabolism at the transcriptional level
    PLOS ONE, 2015
    Co-Authors: Jing Yan, Tianjiao Wang, Kai Liao, Kangsen Mai
    Abstract:

    Ectopic Lipid accumulation has been observed in fish fed a high-Lipid Diet. However, no information is available on the mechanism by which Dietary Lipid levels comprehensively regulate Lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how Dietary Lipids affect Lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a Diet with a low (6%), moderate (12%, the control Diet) or high (18%) crude Lipid content for 10 weeks. Growth performance, plasma biochemical indexes, Lipid contents and gene expression related to Lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-Lipid Diet. In the livers of fish fed the high-Lipid Diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control Diet, and hepatic Lipid deposition increased. In fish fed the low-Lipid Diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control Diet. However, hepatic Lipid content in fish fed the low-Lipid Diet decreased mainly due to low Dietary Lipid intake. In summary, findings of this study provide molecular insight into the role of Lipid deposition in the liver in response to different Dietary Lipid contents.

Tianjiao Wang - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of Cyclooxygenase-2 and its induction pathways in response to high Lipid Diet-induced inflammation in Larmichthys crocea
    Scientific reports, 2016
    Co-Authors: Tianjiao Wang, Jing Yan, Kangsen Mai
    Abstract:

    The present study was conducted to investigate the effects of a high-Lipid Diet (HLD) on cyclooxygenase (Cox)-2 expression and the signalling pathways related to low-grade inflammation in the large yellow croaker (Larmichthys crocea). An isolated 2508 bp cDNA clone of cox-2 contained an open reading frame spanning 1827 bp encoding a protein with 608 amino acid residues. The over-expression of cox-2 was consistent with the activation of c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinase (MAPK) in HLD-fed fish. The activation of the activator protein-1 (AP-1) and the nuclear transcription factor kappa-B (NF-κB) signalling pathways in HLD-fed fish and the significant increase of cox-2 promoter-luciferase activity in vitro indicated that AP-1 and NF-κB could combine cox-2 promoter to promote its transcription, respectively. Together, HLD-induced inflammation up-regulates cox-2 expression via JNKs and p38 MAPK-dependent NF-κB and AP-1 pathways. The present study provides important insight into the signal transduction pathways involved in HLD-induced inflammation, which is detrimental to the health and production of fish as well as to the health of fish consumers.

  • Dietary Lipid levels influence Lipid deposition in the liver of large yellow croaker larimichthys crocea by regulating lipoprotein receptors fatty acid uptake and triacylglycerol synthesis and catabolism at the transcriptional level
    PLOS ONE, 2015
    Co-Authors: Jing Yan, Tianjiao Wang, Kai Liao, Kangsen Mai
    Abstract:

    Ectopic Lipid accumulation has been observed in fish fed a high-Lipid Diet. However, no information is available on the mechanism by which Dietary Lipid levels comprehensively regulate Lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how Dietary Lipids affect Lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a Diet with a low (6%), moderate (12%, the control Diet) or high (18%) crude Lipid content for 10 weeks. Growth performance, plasma biochemical indexes, Lipid contents and gene expression related to Lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-Lipid Diet. In the livers of fish fed the high-Lipid Diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control Diet, and hepatic Lipid deposition increased. In fish fed the low-Lipid Diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control Diet. However, hepatic Lipid content in fish fed the low-Lipid Diet decreased mainly due to low Dietary Lipid intake. In summary, findings of this study provide molecular insight into the role of Lipid deposition in the liver in response to different Dietary Lipid contents.