Longissimus

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Allyson G. Hindle - One of the best experts on this subject based on the ideXlab platform.

  • Skin microbial flora and effectiveness of aseptic technique for deep muscle biopsies in Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica.
    Journal of Wildlife Diseases, 2010
    Co-Authors: Jo-ann E. Mellish, Allyson G. Hindle, Pamela A. Tuomi, Spencer S. Jang, Markus Horning
    Abstract:

    Deep muscle biopsies were collected from the pectoralis and Longissimus dorsi of wild Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica, during October–December 2007. Sterile swabs were collected from the surface of each skin site before biopsy and from the deep-needle path after biopsy. No growth occurred in two of six pectoralis and three of six Longissimus skin sites, or in four of 10 pectoralis deep biopsy and eight of 12 Longissimus deep-biopsy sites. Positive skin culture was not predictive of deep-biopsy contamination, nor did contamination at one body location correlate with contamination at the second site. Psychrobacter species were most common in one or more samples from each of the four sample types. Only one of the eight documented bacteria exhibited resistance to commonly used antibiotics.

  • diving into old age muscular senescence in a large bodied long lived mammal the weddell seal leptonychotes weddellii
    The Journal of Experimental Biology, 2009
    Co-Authors: Allyson G. Hindle, Jo-ann E. Mellish, Markus Horning, John M Lawler
    Abstract:

    SUMMARY Classic aging theory postulates the absence of pronounced organismal senescence in wild animals since mortality probably occurs first. Large-bodied, long-lived mammals are a recognized exception to this tenet, yet organismal senescence has not been investigated to date in such mammals in the wild. Furthermore, oxidative stress theory of aging supports the suggestion that exercise hypoxia, as regularly incurred during apneustic foraging in diving mammals might lead to cellular dysfunction and accelerated aging. To determine if an aspect of organismal senescence occurs in wild marine mammals, we examined the pattern of skeletal muscle aging (contractile and connective tissue components of Longissimus dorsi and pectoralis muscles) in free-ranging adult Weddell seals (9–26 years). The average myocyte cross-sectional area was 22% greater with age in the longissiums dorsi, but no significant increase occurred in the pectoralis. Cross-sectional area was not related to body mass. Changes in myocyte number per area were consistent with the 35–40% age-increase in extracellular space in both muscle groups. Also consistent with extracellular space remodeling, total and relative collagen contents were significantly elevated in older seals (115% in Longissimus dorsi; 65% in pectoralis). The ratio of muscle myocyte to collagen declined with age (50–63%) at both sites. Additionally, a shift towards a higher ratio of type I to type III collagen occurred with advancing age in both muscle groups (79% increase in pectoralis; 49% in Longissimus dorsi). We reject the classic tenet and null-hypothesis that Weddell seals do not survive to an age where muscular senescence becomes detectable.

John M Lawler - One of the best experts on this subject based on the ideXlab platform.

  • diving into old age muscular senescence in a large bodied long lived mammal the weddell seal leptonychotes weddellii
    The Journal of Experimental Biology, 2009
    Co-Authors: Allyson G. Hindle, Jo-ann E. Mellish, Markus Horning, John M Lawler
    Abstract:

    SUMMARY Classic aging theory postulates the absence of pronounced organismal senescence in wild animals since mortality probably occurs first. Large-bodied, long-lived mammals are a recognized exception to this tenet, yet organismal senescence has not been investigated to date in such mammals in the wild. Furthermore, oxidative stress theory of aging supports the suggestion that exercise hypoxia, as regularly incurred during apneustic foraging in diving mammals might lead to cellular dysfunction and accelerated aging. To determine if an aspect of organismal senescence occurs in wild marine mammals, we examined the pattern of skeletal muscle aging (contractile and connective tissue components of Longissimus dorsi and pectoralis muscles) in free-ranging adult Weddell seals (9–26 years). The average myocyte cross-sectional area was 22% greater with age in the longissiums dorsi, but no significant increase occurred in the pectoralis. Cross-sectional area was not related to body mass. Changes in myocyte number per area were consistent with the 35–40% age-increase in extracellular space in both muscle groups. Also consistent with extracellular space remodeling, total and relative collagen contents were significantly elevated in older seals (115% in Longissimus dorsi; 65% in pectoralis). The ratio of muscle myocyte to collagen declined with age (50–63%) at both sites. Additionally, a shift towards a higher ratio of type I to type III collagen occurred with advancing age in both muscle groups (79% increase in pectoralis; 49% in Longissimus dorsi). We reject the classic tenet and null-hypothesis that Weddell seals do not survive to an age where muscular senescence becomes detectable.

Markus Horning - One of the best experts on this subject based on the ideXlab platform.

  • Skin microbial flora and effectiveness of aseptic technique for deep muscle biopsies in Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica.
    Journal of Wildlife Diseases, 2010
    Co-Authors: Jo-ann E. Mellish, Allyson G. Hindle, Pamela A. Tuomi, Spencer S. Jang, Markus Horning
    Abstract:

    Deep muscle biopsies were collected from the pectoralis and Longissimus dorsi of wild Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica, during October–December 2007. Sterile swabs were collected from the surface of each skin site before biopsy and from the deep-needle path after biopsy. No growth occurred in two of six pectoralis and three of six Longissimus skin sites, or in four of 10 pectoralis deep biopsy and eight of 12 Longissimus deep-biopsy sites. Positive skin culture was not predictive of deep-biopsy contamination, nor did contamination at one body location correlate with contamination at the second site. Psychrobacter species were most common in one or more samples from each of the four sample types. Only one of the eight documented bacteria exhibited resistance to commonly used antibiotics.

  • diving into old age muscular senescence in a large bodied long lived mammal the weddell seal leptonychotes weddellii
    The Journal of Experimental Biology, 2009
    Co-Authors: Allyson G. Hindle, Jo-ann E. Mellish, Markus Horning, John M Lawler
    Abstract:

    SUMMARY Classic aging theory postulates the absence of pronounced organismal senescence in wild animals since mortality probably occurs first. Large-bodied, long-lived mammals are a recognized exception to this tenet, yet organismal senescence has not been investigated to date in such mammals in the wild. Furthermore, oxidative stress theory of aging supports the suggestion that exercise hypoxia, as regularly incurred during apneustic foraging in diving mammals might lead to cellular dysfunction and accelerated aging. To determine if an aspect of organismal senescence occurs in wild marine mammals, we examined the pattern of skeletal muscle aging (contractile and connective tissue components of Longissimus dorsi and pectoralis muscles) in free-ranging adult Weddell seals (9–26 years). The average myocyte cross-sectional area was 22% greater with age in the longissiums dorsi, but no significant increase occurred in the pectoralis. Cross-sectional area was not related to body mass. Changes in myocyte number per area were consistent with the 35–40% age-increase in extracellular space in both muscle groups. Also consistent with extracellular space remodeling, total and relative collagen contents were significantly elevated in older seals (115% in Longissimus dorsi; 65% in pectoralis). The ratio of muscle myocyte to collagen declined with age (50–63%) at both sites. Additionally, a shift towards a higher ratio of type I to type III collagen occurred with advancing age in both muscle groups (79% increase in pectoralis; 49% in Longissimus dorsi). We reject the classic tenet and null-hypothesis that Weddell seals do not survive to an age where muscular senescence becomes detectable.

Jo-ann E. Mellish - One of the best experts on this subject based on the ideXlab platform.

  • Skin microbial flora and effectiveness of aseptic technique for deep muscle biopsies in Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica.
    Journal of Wildlife Diseases, 2010
    Co-Authors: Jo-ann E. Mellish, Allyson G. Hindle, Pamela A. Tuomi, Spencer S. Jang, Markus Horning
    Abstract:

    Deep muscle biopsies were collected from the pectoralis and Longissimus dorsi of wild Weddell seals (Leptonychotes weddellii) in McMurdo Sound, Antarctica, during October–December 2007. Sterile swabs were collected from the surface of each skin site before biopsy and from the deep-needle path after biopsy. No growth occurred in two of six pectoralis and three of six Longissimus skin sites, or in four of 10 pectoralis deep biopsy and eight of 12 Longissimus deep-biopsy sites. Positive skin culture was not predictive of deep-biopsy contamination, nor did contamination at one body location correlate with contamination at the second site. Psychrobacter species were most common in one or more samples from each of the four sample types. Only one of the eight documented bacteria exhibited resistance to commonly used antibiotics.

  • diving into old age muscular senescence in a large bodied long lived mammal the weddell seal leptonychotes weddellii
    The Journal of Experimental Biology, 2009
    Co-Authors: Allyson G. Hindle, Jo-ann E. Mellish, Markus Horning, John M Lawler
    Abstract:

    SUMMARY Classic aging theory postulates the absence of pronounced organismal senescence in wild animals since mortality probably occurs first. Large-bodied, long-lived mammals are a recognized exception to this tenet, yet organismal senescence has not been investigated to date in such mammals in the wild. Furthermore, oxidative stress theory of aging supports the suggestion that exercise hypoxia, as regularly incurred during apneustic foraging in diving mammals might lead to cellular dysfunction and accelerated aging. To determine if an aspect of organismal senescence occurs in wild marine mammals, we examined the pattern of skeletal muscle aging (contractile and connective tissue components of Longissimus dorsi and pectoralis muscles) in free-ranging adult Weddell seals (9–26 years). The average myocyte cross-sectional area was 22% greater with age in the longissiums dorsi, but no significant increase occurred in the pectoralis. Cross-sectional area was not related to body mass. Changes in myocyte number per area were consistent with the 35–40% age-increase in extracellular space in both muscle groups. Also consistent with extracellular space remodeling, total and relative collagen contents were significantly elevated in older seals (115% in Longissimus dorsi; 65% in pectoralis). The ratio of muscle myocyte to collagen declined with age (50–63%) at both sites. Additionally, a shift towards a higher ratio of type I to type III collagen occurred with advancing age in both muscle groups (79% increase in pectoralis; 49% in Longissimus dorsi). We reject the classic tenet and null-hypothesis that Weddell seals do not survive to an age where muscular senescence becomes detectable.

B B Marsh - One of the best experts on this subject based on the ideXlab platform.

  • low voltage electrical stimulation of lamb carcasses effects on meat quality
    Meat Science, 1999
    Co-Authors: Paolo Polidori, S Lee, R G Kauffman, B B Marsh
    Abstract:

    The effects of an early post mortem low voltage electrical stimulation (28 V, 60 Hz) on biochemical changes and on final tenderness in muscles Longissimus thoracis et lumborum and Semimembranosus from lamb carcasses were studied. It was shown that electrical stimulation accelerated the glycolytic process resulting in a significant fall in pH during the first 6 h post mortem in both muscles examined and in a significant reduction in adenosine triphosphate (ATP) content in muscle Longissimus thoracis et lumborum. The effect of electrical stimulation on tenderness was recorded by measuring shear force values 2 and 7 days post mortem. Tenderness was significantly improved by electrical stimulation for the Longissimus thoracis et lumborum both at 2 and at 7 days post mortem, while for Semimembranosus electrical stimulation significantly increased tenderness just at 7 days post mortem.