The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Teodoro S Kaufman - One of the best experts on this subject based on the ideXlab platform.
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a multivariate approach for the simultaneous determination of Losartan Potassium and hydrochlorothiazide in a combined pharmaceutical tablet formulation
Analytical and Bioanalytical Chemistry, 2008Co-Authors: Ruben M Maggio, Patricia M Castellano, Teodoro S KaufmanAbstract:A convenient new method for the simultaneous determination of Losartan Potassium and hydrochlorothiazide, with minimum sample pretreatment, is described. The procedure, based on the multivariate analysis of spectral data in the 220−274 nm region by the partial least squares algorithm, is linear in the concentration range 1.06−5.70 mg L−1 for hydrochlorothiazide and 4.0−22.2 mg L−1 for Losartan. It is simple, rapid and robust, allowing accurate and precise results, with drug recovery rates of 99.3 and 100.4% and relative standard deviations of 1.7 and 1.0% obtained for hydrochlorothiazide and Losartan, respectively. The method was applied to the simultaneous determination of both analytes in tablets, and it provided good results which were in statistical agreement with those provided by independent HPLC analyses of the samples. The method has also been successfully applied for the construction of drug dissolution profiles of a commercial pharmaceutical preparation containing both analytes.
Magali Benjamim De Araujo - One of the best experts on this subject based on the ideXlab platform.
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comparative study of analytical methods by direct and first derivative uv spectrophotometry for evaluation of Losartan Potassium in capsules
Brazilian Journal of Pharmaceutical Sciences, 2010Co-Authors: Rudy Bonfilio, Livia Botacini Favoretto, Gislaine Ribeiro Pereira, Roberta De Cassia Pimentel Azevedo, Magali Benjamim De AraujoAbstract:Losartan Potassium is an antihypertensive non-peptide agent, which exerts its action by specific blockade of angiotensin II receptors. The aim of the present study was the validation and application of analytical methods for the quality control of Losartan Potassium 50 mg in pharmaceutical capsules, using direct and first-derivative UV spectrophotometry. Based on Losartan Potassium spectrophotometric characteristics, a signal at 205 nm of the zero-order spectrum and a signal at 234 nm of the first-derivative spectrum, were found adequate for quantification. The results were used to compare these instrumental techniques. The linearity between the signals and concentrations of Losartan Potassium in the ranges of 3.0-7.0 mg L-1 and 6.0-14.0 mg L-1 for direct and first-derivative spectrophotometry in aqueous solutions, respectively, presented a correlation coefficient (r) of 0.9999 in both cases. The methods were applied for Losartan Potassium in capsule dosage obtained from local pharmacies, and were shown to be efficient, easy to apply and low cost. These methods do not use polluting reagents and require relatively inexpensive equipment.
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Losartan Potassium dissolution test for drug release evaluation in pharmaceutical capsules using hplc and uv spectrophotometry
Química Nova, 2010Co-Authors: Rudy Bonfilio, Gislaine Ribeiro Pereira, Magali Benjamim De Araujo, Taciane Ferreira Mendonca, Cesar Ricardo Teixeira TarleyAbstract:This work describes the development and validation of a dissolution test for 50 mg Losartan Potassium capsules using HPLC and UV spectrophotometry. A 24 full factorial design was carried out to optimize dissolution conditions and Potassium phosphate buffer, pH 6.8 as dissolution medium, basket as apparatus at the stirring speed of 50 rpm and time of 30 min were considered adequate. Both dissolution procedure and analytical methods were validated and a statistical analysis showed that there are no significant differences between HPLC and spectrophotometry. Since there is no official monograph, this dissolution test could be applied for quality control routine.
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multivariate optimization and validation of an analytical methodology by rp hplc for the determination of Losartan Potassium in capsules
Talanta, 2009Co-Authors: Rudy Bonfilio, Gislaine Ribeiro Pereira, Cesar Ricardo Teixeira Tarley, Herida Regina Nunes Salgado, Magali Benjamim De AraujoAbstract:Abstract This paper describes the optimization and validation of an analytical methodology for the determination of Losartan Potassium in capsules by HPLC using 2 5-1 fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized Potassium phosphate buffer (pH 6.2; 58 mmol L −1 )–acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min −1 . An octylsilane column (100 mm × 4.6 mm i.d., 5 μm) maintained at 35 °C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values r = 0.9998) over a concentration range from 30 to 70 mg L −1 of Losartan Potassium. The limits of detection and quantification were 0.114 and 0.420 mg L −1 , respectively. The validated method may be used to quantify Losartan Potassium in capsules and to determine the stability of this drug.
Renato F Perez - One of the best experts on this subject based on the ideXlab platform.
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development and validation of an uv derivative spectrophotometric determination of Losartan Potassium in tablets
Journal of Pharmaceutical and Biomedical Analysis, 2003Co-Authors: Olga Lastra, Igor Lemus, Hugo Sanchez, Renato F PerezAbstract:Abstract Development and validation of an analytical UV derivative spectrophotometric method to quantify Losartan Potassium used as a single active principle in pharmaceutical forms were done. Pharmacopeias have not yet provided an official method for its quantification. A study was carried out of all the parameters established by USP XXIV to validate an analytical method for a solid pharmaceutical form, i.e. linearity, range, accuracy, precision and specificity. All these parameters were found in accordance with the acceptance criteria of Comite de Guias Oficiales de Validacion de la Direccion General de Control de Insumos para la Salud de Mexico. Based on the spectrophotometric characteristics of Losartan Potassium, a signal at 234 nm of the first derivative spectrum (1D 234 ) was found adequate for quantification. The linearity between signal 1D 234 and concentration of Losartan Potassium in the range of 4.00–6.00 mg l −1 in aqueous solutions presents a square correlation coefficient (r 2 ) of 0.9938. The mean recovery percentage was 100.7±1.1% and the precision expressed as relative standard deviation (R.S.D.) 0.88%. In addition, the proposed method is simple, easy to apply, low-cost, does not use polluting reagents and requires relatively inexpensive instruments. Then, it is a good alternative to existing methods for determining Losartan Potassium in tablets provided that the pharmaceutical dosage form does not contain hydrochlorothiazide as second drug.
Rudy Bonfilio - One of the best experts on this subject based on the ideXlab platform.
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comparative study of analytical methods by direct and first derivative uv spectrophotometry for evaluation of Losartan Potassium in capsules
Brazilian Journal of Pharmaceutical Sciences, 2010Co-Authors: Rudy Bonfilio, Livia Botacini Favoretto, Gislaine Ribeiro Pereira, Roberta De Cassia Pimentel Azevedo, Magali Benjamim De AraujoAbstract:Losartan Potassium is an antihypertensive non-peptide agent, which exerts its action by specific blockade of angiotensin II receptors. The aim of the present study was the validation and application of analytical methods for the quality control of Losartan Potassium 50 mg in pharmaceutical capsules, using direct and first-derivative UV spectrophotometry. Based on Losartan Potassium spectrophotometric characteristics, a signal at 205 nm of the zero-order spectrum and a signal at 234 nm of the first-derivative spectrum, were found adequate for quantification. The results were used to compare these instrumental techniques. The linearity between the signals and concentrations of Losartan Potassium in the ranges of 3.0-7.0 mg L-1 and 6.0-14.0 mg L-1 for direct and first-derivative spectrophotometry in aqueous solutions, respectively, presented a correlation coefficient (r) of 0.9999 in both cases. The methods were applied for Losartan Potassium in capsule dosage obtained from local pharmacies, and were shown to be efficient, easy to apply and low cost. These methods do not use polluting reagents and require relatively inexpensive equipment.
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Losartan Potassium dissolution test for drug release evaluation in pharmaceutical capsules using hplc and uv spectrophotometry
Química Nova, 2010Co-Authors: Rudy Bonfilio, Gislaine Ribeiro Pereira, Magali Benjamim De Araujo, Taciane Ferreira Mendonca, Cesar Ricardo Teixeira TarleyAbstract:This work describes the development and validation of a dissolution test for 50 mg Losartan Potassium capsules using HPLC and UV spectrophotometry. A 24 full factorial design was carried out to optimize dissolution conditions and Potassium phosphate buffer, pH 6.8 as dissolution medium, basket as apparatus at the stirring speed of 50 rpm and time of 30 min were considered adequate. Both dissolution procedure and analytical methods were validated and a statistical analysis showed that there are no significant differences between HPLC and spectrophotometry. Since there is no official monograph, this dissolution test could be applied for quality control routine.
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multivariate optimization and validation of an analytical methodology by rp hplc for the determination of Losartan Potassium in capsules
Talanta, 2009Co-Authors: Rudy Bonfilio, Gislaine Ribeiro Pereira, Cesar Ricardo Teixeira Tarley, Herida Regina Nunes Salgado, Magali Benjamim De AraujoAbstract:Abstract This paper describes the optimization and validation of an analytical methodology for the determination of Losartan Potassium in capsules by HPLC using 2 5-1 fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized Potassium phosphate buffer (pH 6.2; 58 mmol L −1 )–acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min −1 . An octylsilane column (100 mm × 4.6 mm i.d., 5 μm) maintained at 35 °C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values r = 0.9998) over a concentration range from 30 to 70 mg L −1 of Losartan Potassium. The limits of detection and quantification were 0.114 and 0.420 mg L −1 , respectively. The validated method may be used to quantify Losartan Potassium in capsules and to determine the stability of this drug.
Rajani Giridhar - One of the best experts on this subject based on the ideXlab platform.
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a rapid colorimetric method for the determination of Losartan Potassium in bulk and in synthetic mixture for solid dosage form
Journal of Pharmaceutical and Biomedical Analysis, 2002Co-Authors: Anandkumari H Prabhakar, Rajani GiridharAbstract:Abstract Two new rapid reproducible and economical spectrophotometric methods are described for the determination of Losartan Potassium in bulk and in synthetic mixture for solid dosage forms. Both methods are based on the formation of an orange-red and orange ion-pair complex due to the action of Calmagite (CT) and Orange-II (O-II) on Losartan Potassium in acidic medium (pH 1.2). Under optimised conditions, they show an absorption maxima at 491 nm (CT) and 486 nm (O-II), with molar absorptivities of 1.74×103 and 1.75×103 l mol−1 cm−1 and Sandell's sensitivities of 0.2649 and 0.2637 per 0.001 absorbance unit for CT and O-II, respectively. The colour is stable for 5 min after extraction. In both cases Beer's law is obeyed between 10 and 100 μg ml−1. The proposed method was successfully extended to synthetic mixture for solid dosage forms.
