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Alvaro Peix - One of the best experts on this subject based on the ideXlab platform.
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mesorhizobium helmanticense sp nov isolated from Lotus Corniculatus nodules
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Marta Marcosgarcia, Marta Helena Ramirezbahena, Encarna Velázquez, Alvaro Peix, Esther Menendez, Pedro F Mateos, Raul RivasAbstract:In this study, three strains belonging to the genus Mesorhizobium , CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus Corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesorhizobium gobiense CCBAU 83330T and Mesorhizobium caraganae CCBAU 11299T with similarity values higher than 99.7 %. The analysis of concatenated recA and glnII genes showed that the most closely related type strains were M. metallidurans STM 2683T, M. tianshanense A-1BST and M. tarimense CCBAU 83306T with 96, 95 and 94 % similarity values in the recA gene and 95, 94 and 94 % in the glnII gene, respectively. M. metallidurans LMG 24485T, M. tianshanense USDA 3592T and M. tarimense LMG 24338T showed means of 44, 41 and 42 % DNA–DNA relatedness, respectively, with respect to strain CSLC115NT. The major fatty acids were those from summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and C18 : 1ω7c 11-methyl. The results of phenotypic characterization support that the L. Corniculatus nodulating strains analysed in this work belong to a novel species of the genus Mesorhizobium for which the name Mesorhizobium helmanticense sp. nov. is proposed, and the type strain is CSLC115NT (= LMG 29734T=CECT 9168T).
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mesorhizobium olivaresii sp nov isolated from Lotus Corniculatus nodules
Systematic and Applied Microbiology, 2016Co-Authors: Maria J Lorite, Alvaro Peix, Jose David Floresfelix, Juan Sanjuán, Encarna VelázquezAbstract:Abstract In this study four Mesorhizobium strains isolated from Lotus Corniculatus nodules in Granada (Spain) were characterized. Their 16S rRNA gene sequences were closely related to those of M. albiziae LMG 23507T and M. chacoense Pr5T showing 99.4 and 99.2% similarity values, respectively. The analysis of concatenated rpoB, recA, atpD and glnII genes showed they formed a cluster with internal similarities higher than 97%. The closest species also were M. albiziae LMG 23507T and M. chacoense Pr5T showing similarity values lower than 92% in rpoB, recA and glnII genes and lower than 96.5% in the atpD gene. These results indicated that the L. Corniculatus strains belong to a new species of genus Mesorhizobium which was confirmed by DNA–DNA hybridization and phenotypic characterization. Therefore a new species with the name Mesorhizobium olivaresii sp. nov. is proposed, and the type strain is CPS13T (LMG 29295T = CECT 9099T).
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Phyllobacterium loti sp. nov. isolated from nodules of Lotus Corniculatus
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Maximo Sánchez, Encarna Velázquez, Maria J Lorite, Alvaro Peix, Juan Sanjuán, Martha-helena Ramírez-bahena, Jorge MonzaAbstract:Strain S658T was isolated from a Lotus Corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus Corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).
Encarna Velázquez - One of the best experts on this subject based on the ideXlab platform.
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mesorhizobium helmanticense sp nov isolated from Lotus Corniculatus nodules
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Marta Marcosgarcia, Marta Helena Ramirezbahena, Encarna Velázquez, Alvaro Peix, Esther Menendez, Pedro F Mateos, Raul RivasAbstract:In this study, three strains belonging to the genus Mesorhizobium , CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus Corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesorhizobium gobiense CCBAU 83330T and Mesorhizobium caraganae CCBAU 11299T with similarity values higher than 99.7 %. The analysis of concatenated recA and glnII genes showed that the most closely related type strains were M. metallidurans STM 2683T, M. tianshanense A-1BST and M. tarimense CCBAU 83306T with 96, 95 and 94 % similarity values in the recA gene and 95, 94 and 94 % in the glnII gene, respectively. M. metallidurans LMG 24485T, M. tianshanense USDA 3592T and M. tarimense LMG 24338T showed means of 44, 41 and 42 % DNA–DNA relatedness, respectively, with respect to strain CSLC115NT. The major fatty acids were those from summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and C18 : 1ω7c 11-methyl. The results of phenotypic characterization support that the L. Corniculatus nodulating strains analysed in this work belong to a novel species of the genus Mesorhizobium for which the name Mesorhizobium helmanticense sp. nov. is proposed, and the type strain is CSLC115NT (= LMG 29734T=CECT 9168T).
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mesorhizobium olivaresii sp nov isolated from Lotus Corniculatus nodules
Systematic and Applied Microbiology, 2016Co-Authors: Maria J Lorite, Alvaro Peix, Jose David Floresfelix, Juan Sanjuán, Encarna VelázquezAbstract:Abstract In this study four Mesorhizobium strains isolated from Lotus Corniculatus nodules in Granada (Spain) were characterized. Their 16S rRNA gene sequences were closely related to those of M. albiziae LMG 23507T and M. chacoense Pr5T showing 99.4 and 99.2% similarity values, respectively. The analysis of concatenated rpoB, recA, atpD and glnII genes showed they formed a cluster with internal similarities higher than 97%. The closest species also were M. albiziae LMG 23507T and M. chacoense Pr5T showing similarity values lower than 92% in rpoB, recA and glnII genes and lower than 96.5% in the atpD gene. These results indicated that the L. Corniculatus strains belong to a new species of genus Mesorhizobium which was confirmed by DNA–DNA hybridization and phenotypic characterization. Therefore a new species with the name Mesorhizobium olivaresii sp. nov. is proposed, and the type strain is CPS13T (LMG 29295T = CECT 9099T).
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The high diversity of Lotus Corniculatus endosymbionts in soils of northwest Spain.
Symbiosis, 2015Co-Authors: Marta Marcos-garcía, Encarna Velázquez, Esther Menendez, Pedro F Mateos, Xavier Cruz-gonzález, Raul RivasAbstract:The diversity of rhizobia that establish symbiosis with Lotus Corniculatus has scarcely been studied. Several species of Mesorhizobium are endosymbionts of this legume, including Mesorhizobium loti, the type species of this genus. We analysed the genetic diversity of strains nodulating Lotus Corniculatus in Northwest Spain and ten different RAPD patterns were identified among 22 isolates. The phylogenetic analysis of the 16S rRNA gene showed that the isolated strains belong to four divergent phylogenetic groups within the genus Mesorhizobium. These phylogenetic groups are widely distributed worldwide and the strains nodulate L. Corniculatus in several countries of Europe, America and Asia. Three of the groups include the currently described Mesorhizobium species M. loti, M. erdmanii and M. jarvisii which are L. Corniculatus endosymbionts. An analysis of the recA and atpD genes showed that our strains belong to several clusters, one of them very closely related to M. jarvisii and the remanining ones phylogenetically divergent from all currently described Mesorhizobium species. Some of these clusters include L. Corniculatus nodulating strains isolated in Europe, America and Asia, although the recA and atpD genes have been sequenced in only a few L. Corniculatus endosymbionts. The results of this study revealed great phylogenetic diversity of strains nodulating L. Corniculatus, allowing us to predict that even more diversity will be discovered as further ecosystems are investigated.
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Phyllobacterium loti sp. nov. isolated from nodules of Lotus Corniculatus
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Maximo Sánchez, Encarna Velázquez, Maria J Lorite, Alvaro Peix, Juan Sanjuán, Martha-helena Ramírez-bahena, Jorge MonzaAbstract:Strain S658T was isolated from a Lotus Corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus Corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).
Phillip Morris - One of the best experts on this subject based on the ideXlab platform.
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Bacterial colonisation of the tanniferous forage Lotus Corniculatus in the bovine rumen
2010Co-Authors: Phillip Morris, Mark P. Robbins, Joan E. Edwards, Alison H. Kingston-smith, Eun Joong KimAbstract:Edwards. J. E., Morris, P., Robbins, M. P., Kim, E. J., Kingston-Smith, A. H. (2009). Bacterial colonisation of the tanniferous forage Lotus Corniculatus in the bovine rumen. Microbial Ecology. Proceedings of the 2009 Conference on Gastrointestinal Function, Chicago, USA, April 20-22 2009, 57, (3), 569-570. Proceedings of the 2009 Conference on Gastrointestinal Function, Chicago, USA, April 20-22 2009
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sn a maize bhlh gene modulates anthocyanin and condensed tannin pathways in Lotus Corniculatus
Journal of Experimental Botany, 2003Co-Authors: Mark P. Robbins, Phillip Morris, Francesco Paolocci, Johnwayne Hughes, Valentina Turchetti, Gordon G Allison, Sergio Arcioni, F DamianiAbstract:Robbins, M. P., Paolocci, F., Hughes, J-W., Turchetti, V., Allison, G. G., Arcioni, S., Morris, P., Damiani, F. (2003). Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus Corniculatus. Journal of Experimental Botany, 54, (381), 239-248. Sponsorship: BBSRC/Consiglio Nazionale della Richerche.
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Responses of Lotus Corniculatus to environmental change
New Phytologist, 1997Co-Authors: Eunice B. Carter, Michael K. Theodorou, Phillip MorrisAbstract:SUMMARY Five clonal plants of three genotypes of Lotus Corniculatus were grown in each of eight controlled environments under combinations of two temperature regimes (18/10 °C and 25/15 °C), two COg concentrations (ambient and 700 ppmv) and two water applications {ad libitum or 60% droughted). Plants were harvested at full flower and measurements made of plant growth and development. Of the three environmental variables studied, higher growth temperatures resulted in the largest number of significant changes to the measured variables. Reproductive capacity, growth rate, shoot biomass, water use efficiency and chlorophyll content were all enhanced by raising the growth temperature from 18 to 25 °C. Doubling the COj concentration enhanced the growth rate, shoot biomass and water use efficiency and ameliorated some of the eflFects of drought, including reproductive capacity, and biomass production, but reduced fiowering time, specific leaf area, and chlorophyll content of both droughted and undroughted plants. Drought alone reduced reproductive capacity, growth rate and above ground biomass but significantly increased root biomass in all environments. The agronomic eflFects resulting from a combined increase in growth temperature, doubled CO^ concentration and mild drought in this experiment were a shorter vegetative period and an increase in biomass, but a fall in reproductive capacity.
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Phenylpropanoid defence responses in transgenic Lotus Corniculatus 1. Glutathione elicitation of isoflavan phytoalexins in transformed root cultures
Plant cell reports, 1991Co-Authors: M. P. Robbins, J. Hartnoll, Phillip MorrisAbstract:When Agrobacterium rhizogenes transformed root cultures of Lotus Corniculatus were treated with glutathione, isoflavan phytoalexins accumulated in both tissue and culture medium. This accumulation of phytoalexins was preceded by a transient increase in the activity of phenylalanine ammonia lyase (PAL). Elicitation of PAL occurred throughout the growth curve of Lotus ‘hairy roots’ and in different sectors of transformed root material.
Jorge Monza - One of the best experts on this subject based on the ideXlab platform.
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Phyllobacterium loti sp. nov. isolated from nodules of Lotus Corniculatus
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Maximo Sánchez, Encarna Velázquez, Maria J Lorite, Alvaro Peix, Juan Sanjuán, Martha-helena Ramírez-bahena, Jorge MonzaAbstract:Strain S658T was isolated from a Lotus Corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus Corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).
Maria J Lorite - One of the best experts on this subject based on the ideXlab platform.
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mesorhizobium olivaresii sp nov isolated from Lotus Corniculatus nodules
Systematic and Applied Microbiology, 2016Co-Authors: Maria J Lorite, Alvaro Peix, Jose David Floresfelix, Juan Sanjuán, Encarna VelázquezAbstract:Abstract In this study four Mesorhizobium strains isolated from Lotus Corniculatus nodules in Granada (Spain) were characterized. Their 16S rRNA gene sequences were closely related to those of M. albiziae LMG 23507T and M. chacoense Pr5T showing 99.4 and 99.2% similarity values, respectively. The analysis of concatenated rpoB, recA, atpD and glnII genes showed they formed a cluster with internal similarities higher than 97%. The closest species also were M. albiziae LMG 23507T and M. chacoense Pr5T showing similarity values lower than 92% in rpoB, recA and glnII genes and lower than 96.5% in the atpD gene. These results indicated that the L. Corniculatus strains belong to a new species of genus Mesorhizobium which was confirmed by DNA–DNA hybridization and phenotypic characterization. Therefore a new species with the name Mesorhizobium olivaresii sp. nov. is proposed, and the type strain is CPS13T (LMG 29295T = CECT 9099T).
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Phyllobacterium loti sp. nov. isolated from nodules of Lotus Corniculatus
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Maximo Sánchez, Encarna Velázquez, Maria J Lorite, Alvaro Peix, Juan Sanjuán, Martha-helena Ramírez-bahena, Jorge MonzaAbstract:Strain S658T was isolated from a Lotus Corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus Corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).
