The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Junjie Zhu - One of the best experts on this subject based on the ideXlab platform.
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a novel aptasensor for lysozyme based on electrogenerated chemiluminescence resonance energy transfer between Luminol and silicon quantum dots
Biosensors and Bioelectronics, 2017Co-Authors: Yongping Dong, Jiao Wang, Ying Peng, Junjie ZhuAbstract:Abstract In the present work, electrogenerated chemiluminescence (ECL) of Luminol was investigated in neutral condition at a gold electrode in the presence of silicon quantum dots (SiQDs). The results revealed that SiQDs can not only greatly enhance Luminol ECL, but also act as energy acceptor to construct a novel ECL resonance energy transfer (ECL-RET) system with Luminol. As a result, strong anodic ECL signal was obtained in neutral condition at the bare gold electrode, which is suitable for biosensing application. Lysozyme exhibited apparent inhibiting effect on the ECL-RET system, based on which an ECL aptasensor was fabricated for the sensitive detection of lysozyme. The proposed method showed high sensitivity, good selectivity, and wide linearity for the detection of lysozyme in the range of 5.0×10 –14 −5.0×10 −9 g mL −1 with a detection limit of 5.8×10 –15 g mL −1 (3σ). The results suggested that as-proposed Luminol/SiQDs ECL biosensor will be promising in the detection enzyme.
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electrogenerated chemiluminescence resonance energy transfer between Luminol and cdse zns quantum dots and its sensing application in the determination of thrombin
Analytical Chemistry, 2014Co-Authors: Yongping Dong, Ying Zhou, Tingting Gao, Junjie ZhuAbstract:In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between Luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the Luminol oxidation to promote the anodic Luminol ECL without coreactants. The intensity of anodic Luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of Luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM–100 pM with a detection limit of 1.4 fM (S/N = 3). The result...
Hua Cui - One of the best experts on this subject based on the ideXlab platform.
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label free electrochemiluminescence immunosensor for cardiac troponin i using Luminol functionalized gold nanoparticles as a sensing platform
Analyst, 2013Co-Authors: Hua Cui, Di Yang, Zhiping BianAbstract:A simple and sensitive label-free electrochemiluminescence (ECL) immunosensor based on the use of Luminol functionalized gold nanoparticles (Luminol-AuNPs) as antibody carriers and sensing platform is described for detecting the acute myocardial infarction biomarker cTnI. The ECL immunosensor was fabricated by the assembly of Luminol-AuNPs conjugated with biotinylated antibodies against cTnI (biotin-anti-cTnI-Luminol-AuNPs) with the streptavidin coated AuNPs (SA-AuNPs) modified Au electrode directly by virtue of the biotin-SA system. The fabricated sensing platform exhibited stable and strong ECL intensity and could be used for the recognition of target antigen. In the presence of cTnI, a decrease in the ECL intensity was observed. Direct detection of the ECL signal changes during antigen-antibody immunoreactions can be used for the quantification of cTnI. The ECL response exhibited a quite wide dynamic range from 1000 ng mL(-1) down to 0.1 ng mL(-1). The proposed method has been successfully applied in the detection of cTnI in real plasma samples. This protocol is simple, fast, sensitive, specific, stable and reliable. This work reveals that the Luminol-AuNPs are excellent sensing platforms for the fabrication of simple and sensitive immunosensors. Moreover, the proposed strategy may also be extended for the detection of other biomarkers, which is of great application potential in clinical and pharmaceutical analysis.
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a label free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and Luminol functionalized gold nanoparticles
Biosensors and Bioelectronics, 2013Co-Authors: Hua CuiAbstract:Abstract In the work, a label-free electrochemiluminescence (ECL) aptasensor for the sensitive and selective detection of thrombin was constructed based on target-induced direct ECL signal change by virtue of a novel assembly strategy of oligonucleotide and Luminol functionalized gold nanoparticles (Luminol-AuNPs). It is the first label-free ECL biosensor based on Luminol and its analogs functionalized AuNPs. Streptavidin AuNPs coated with biotinylated DNA capture probe 1 (AuNPs–probe 1) were firstly assembled onto an gold electrode through 1,3-propanedithiol. Then Luminol-AuNPs co-loaded with thiolated DNA capture probe 2 and thiolated thrombin binding aptamer (TBA) (Luminol-AuNPs–probe 2/TBA) were assembled onto AuNPs–probe 1 modified electrode through the hybridization between capture probes 1 and 2. The Luminol-AuNPs–probe 2/TBA acted as both molecule recognition probe and sensing interface. An Au/AuNPs/ds-DNA/Luminol-AuNPs/TBA multilayer architecture was obtained. In the presence of target thrombin, TBA on the Luminol-AuNPs could capture the thrombin onto the electrode surface, which produced a barrier for electro-transfer and influenced the electro-oxidation reaction of Luminol, leading to a decrease in ECL intensity. The change of ECL intensity indirectly reflected the concentration of thrombin. Thus, the approach showed a high sensitivity and a wider linearity for the detection of thrombin in the range of 0.005–50 nM with a detection limit of 1.7 pM. This work reveals that Luminol-AuNPs are ideal platform for label-free ECL bioassays.
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gold nanoparticle triggered chemiluminescence between Luminol and agno3
Journal of Physical Chemistry C, 2008Co-Authors: Hua Cui, Jizhao Guo, Lijia LiuAbstract:It was found that Luminol could react with AgNO 3 in the presence of gold colloid to generate chemiluminescence (CL) at 425 nm. UV−visible spectra and X-ray photoelectron spectra showed that AgNO 3 was reduced by Luminol to Ag in the CL reaction, which covered on the surface of gold nanoparticles to form Au/Ag core/shell nanoparticles. The luminophor was identified by the CL spectrum as 3-aminophthalate. The effects of Au/Ag core/shell nanoparticles with various compositions on the Luminol−AgNO 3 system and the CL kinetics were also studied. On this basis, a CL reaction mechanism involving catalysis is proposed. When Au colloid was injected into the mixture of Luminol and AgNO 3, AgNO 3 reacted rapidly with Luminol under the catalysis of gold nanoparticles to produce Ag and Luminol radicals, which reacted with the dissolved oxygen, accompanying a light emission; then, with instant deposition of Ag atoms on the surface of Au particles, the catalytic activity declined, leading to a sharp decrease in CL inte...
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ag nanoparticle catalyzed chemiluminescent reaction between Luminol and hydrogen peroxide
Journal of Photochemistry and Photobiology A-chemistry, 2008Co-Authors: Jizhao Guo, Hua Cui, Wei Zhou, Wei WangAbstract:Abstract Ag colloid was found to enhance intensely the chemiluminescence (CL) from the reaction between Luminol and hydrogen peroxide. Ag nanoparticles exhibited the better CL catalysis activity than gold and platinum nanoparticles. The superoxide anion scavenger nitro blue tetrazolium and superoxide dismutase was added to the hydrogen peroxide–Ag colloid and the Luminol–hydrogen peroxide–Ag colloid systems, respectively, showing that the decomposition of hydrogen peroxide by catalysis of silver nanoparticles formed superoxide anion and superoxide anion was involved in Luminol–hydrogen peroxide–Ag colloid CL reaction. The Ag nanoparticle-enhanced CL was ascribed to that Ag nanoparticles could catalyze the decomposition of H2O2 to produce some reactive intermediates such as hydroxyl radical, superoxide anion. Hydroxyl radical reacted with Luminol to form Luminol radical and diazaquinone, followed by the reaction with superoxide anion or monodissociated hydrogen peroxide, giving rising to light emission. Halide ions (X−) were found to quench the CL in the following order: I− > Br− > Cl−, due to the formation of AgX shell on Ag nanoparticles surface which poisoned the Ag catalyst. An obvious turning point was observed in the curve of CL intensity versus iodine ion concentration, which corresponded to the I− concentration needed for mono-layer saturation adsorption on the Ag nanoparticles. A chemical adsorption model for iodine ions on the surface of Ag colloids has been proposed. Among 20 natural amino acids, cysteine, histidine, methionine, tyrosine and tryptophan were found to inhibit the CL due to their adsorption on the Ag nanoparticles and their competitive consumption for the reactive intermediates. The most intense inhibition of cysteine may be of potential for selective determination of cysteine.
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synthesis characterization and electrochemiluminescence of Luminol reduced gold nanoparticles and their application in a hydrogen peroxide sensor
Chemistry: A European Journal, 2007Co-Authors: Hua Cui, Yongping Dong, Wei Wang, Chunfeng Duan, Jizhao GuoAbstract:It was found that chloroauric acid (HAuCl 4 ) could be directly reduced by the luminescent reagent Luminol in aqueous solution to form gold nanoparticles (AuNPs), the size of which depended on the amount of Luminol. The morphology and surface state of as-prepared AuNPs were characterized by transmission electron microscopy, UV/visible spectroscopy, X-ray photoelectron spectroscopy, FTIR spectroscopy, and thermogravimetric analysis. All results indicated that residual Luminol and its oxidation product 3-aminophthalate coexisted on the surface of AuNPs through the weak covalent interaction between gold and nitrogen atoms in their amino groups. Subsequently, a Luminol-capped AuNP-modified electrode was fabricated by the immobilization of AuNPs on a gold electrode by virtue of cysteine molecules and then immersion in a Luminol solution. The modified electrode was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. The as-prepared modified electrode exhibited an electrochemiluminescence (ECL) response in alkaline aqueous solution under a double-step potential. H 2 O 2 was found to enhance the ECL. On this basis, an ECL sensor for the detection of H 2 O 2 was developed. The method is simple, fast, and reagent free. It is applicable to the determination of H 2 O 2 in the range of 3x10 -7 -1x 10 -3 mol L -1 with a detection limit of 1x10 -7 molL -1 (S/N=3).
Yongping Dong - One of the best experts on this subject based on the ideXlab platform.
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a novel aptasensor for lysozyme based on electrogenerated chemiluminescence resonance energy transfer between Luminol and silicon quantum dots
Biosensors and Bioelectronics, 2017Co-Authors: Yongping Dong, Jiao Wang, Ying Peng, Junjie ZhuAbstract:Abstract In the present work, electrogenerated chemiluminescence (ECL) of Luminol was investigated in neutral condition at a gold electrode in the presence of silicon quantum dots (SiQDs). The results revealed that SiQDs can not only greatly enhance Luminol ECL, but also act as energy acceptor to construct a novel ECL resonance energy transfer (ECL-RET) system with Luminol. As a result, strong anodic ECL signal was obtained in neutral condition at the bare gold electrode, which is suitable for biosensing application. Lysozyme exhibited apparent inhibiting effect on the ECL-RET system, based on which an ECL aptasensor was fabricated for the sensitive detection of lysozyme. The proposed method showed high sensitivity, good selectivity, and wide linearity for the detection of lysozyme in the range of 5.0×10 –14 −5.0×10 −9 g mL −1 with a detection limit of 5.8×10 –15 g mL −1 (3σ). The results suggested that as-proposed Luminol/SiQDs ECL biosensor will be promising in the detection enzyme.
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electrogenerated chemiluminescence resonance energy transfer between Luminol and cdse zns quantum dots and its sensing application in the determination of thrombin
Analytical Chemistry, 2014Co-Authors: Yongping Dong, Ying Zhou, Tingting Gao, Junjie ZhuAbstract:In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between Luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the Luminol oxidation to promote the anodic Luminol ECL without coreactants. The intensity of anodic Luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of Luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM–100 pM with a detection limit of 1.4 fM (S/N = 3). The result...
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synthesis characterization and electrochemiluminescence of Luminol reduced gold nanoparticles and their application in a hydrogen peroxide sensor
Chemistry: A European Journal, 2007Co-Authors: Hua Cui, Yongping Dong, Wei Wang, Chunfeng Duan, Jizhao GuoAbstract:It was found that chloroauric acid (HAuCl 4 ) could be directly reduced by the luminescent reagent Luminol in aqueous solution to form gold nanoparticles (AuNPs), the size of which depended on the amount of Luminol. The morphology and surface state of as-prepared AuNPs were characterized by transmission electron microscopy, UV/visible spectroscopy, X-ray photoelectron spectroscopy, FTIR spectroscopy, and thermogravimetric analysis. All results indicated that residual Luminol and its oxidation product 3-aminophthalate coexisted on the surface of AuNPs through the weak covalent interaction between gold and nitrogen atoms in their amino groups. Subsequently, a Luminol-capped AuNP-modified electrode was fabricated by the immobilization of AuNPs on a gold electrode by virtue of cysteine molecules and then immersion in a Luminol solution. The modified electrode was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. The as-prepared modified electrode exhibited an electrochemiluminescence (ECL) response in alkaline aqueous solution under a double-step potential. H 2 O 2 was found to enhance the ECL. On this basis, an ECL sensor for the detection of H 2 O 2 was developed. The method is simple, fast, and reagent free. It is applicable to the determination of H 2 O 2 in the range of 3x10 -7 -1x 10 -3 mol L -1 with a detection limit of 1x10 -7 molL -1 (S/N=3).
Shanshan Wang - One of the best experts on this subject based on the ideXlab platform.
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a highly sensitive and stable electrochemiluminescence immunosensor for alpha fetoprotein detection based on Luminol agnps co ni mof nanosheet microflowers
Sensors and Actuators B-chemical, 2020Co-Authors: Shanshan Wang, Minmin Wang, Xiangdong Zhang, Yongdong JinAbstract:Abstract Due to their high catalytic effect on the electrochemiluminescence (ECL) of Luminol, noble metal nanoparticles (NPs) have been widely used in Luminol-based ECL immunosensors. However the aggregation of NPs often resulted in the decrease of ECL signal and affected the stability and durability of the sensor. In this study, a microflower-like structure made of ultrathin Co/Ni-based metal-organic frameworks (MOF) nanosheets were used as a platform for Luminol-functionalized AgNPs to construct an immunosensor for tumor marker alpha-fetoprotein. The nanosheet and microflower-like assembly structure of the Co/Ni-MOF greatly improved the ECL performance of the Luminol-AgNPs system, attributed to their large surface area, resistance to particle agglomeration and the high catalytic activity of the Co/Ni-MOF. The ultrathin Co/Ni-MOF was full of atomically dispersed cobalt ions and nickel ions, which can catalyze the ECL reaction of Luminol and H2O2. The as-fabricated immunosensor has a good sensitivity with detection limit of 0.417 pg mL−1 (S/N=3), and shows satisfactory performance in practical applications.
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enhancing Luminol electrochemiluminescence by combined use of cobalt based metal organic frameworks and silver nanoparticles and its application in ultrasensitive detection of cardiac troponin i
Analytical Chemistry, 2019Co-Authors: Muhammad Saqib, Shanshan Wang, Minmin Wang, Xiangdong Zhang, Yangyang Zhao, Yongdong JinAbstract:Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardiac troponin I (cTnI) for cardiac infarction diagnosis. In this study, an effective tactic was developed to enhance ECL efficiency of the Luminol system, by combined use of Co2+-based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and Luminol-capped Ag nanoparticles (Luminol-AgNPs). The integration leads to a pronounced ∼115-fold enhancement in Luminol ECL. On the basis of this fascinating sensing platform, a robust label-free ECL immunosensor was constructed for ultrasensitive detection of cTnI, the main marker of myocardial infarction, with good stability and a detection limit as low as 0.58 fg mL-1 (S/N = 3).
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Study on Enhancement Principle and Stabilization for the Luminol-H2O2-HRP Chemiluminescence System
2015Co-Authors: Lihua Yang, Maojun Jin, Ge Chen, Chan Zhang, Fen Jin, Hua Shao, Yongxin She, Jian Wang, Shanshan WangAbstract:A Luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU) and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the Luminol-H2O2-HRP chemiluminescence system was carried out, and the results showed that 4-(imidazol-1-yl)phenol (4-IMP), 4-iodophenol (4-IOP), 4-bromophenol (4-BOP) and 4-hydroxy-4’-iodobiphenyl (HIOP) had the best performance. Based on the experiment, the four enhancers were dissolved in acetone, acetonitrile, methanol, and dimethylformamide (DMF) with various concentrations, the results indicated that 4-IMP, 4-IOP, 4-BOP and HIOP dissolved in DMF with the concentrations of 0.2%, 3.2%, 1.6% and 3.2% could get the highest RLU values. Subsequently, the influences of pH, ionic strength, HRP, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and Luminol on the stabilization of the Luminol-H2O2-HRP chemiluminescence system were studied, and we found that pH value, ionic strength, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and Luminol have little influence on luminescent stabilization, while HRP has a great influence. In different ranges of HRP concentration, different enhancers should be selected. When the concentration is within the range of 0~6 ng/mL, 4-IMP should be selected. When the concentration of HRP ranges from 6 to 25ng/mL, 4-IOP was the best choice. And when the concentration is within the range of 25~80 ng/mL, HIOP should be selected as the enhancer. Finally, the three well-performing chemiluminescent enhanced solutions (CESs) have been further optimized according to the three enhancers (4-IMP, 4-IOP and HIOP) in their utilized HRP concentration ranges.
Yaqin Chai - One of the best experts on this subject based on the ideXlab platform.
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highly efficient Luminol immobilization approach and exponential strand displacement reaction based electrochemiluminescent strategy for monitoring microrna expression in cell
Biosensors and Bioelectronics, 2019Co-Authors: Yali Yuan, Haijun Wang, Yaqin Chai, Anyi Chen, Ruo YuanAbstract:Abstract This work used 3,4,9,10-perylenetetracarboxylic acid-Luminol composite (PTCA-Luminol) as signal tag with improved ECL signal and applied cruciform DNA structure mediated exponential strand displacement reaction (SDR) to construct an ultrasensitive electrochemiluminescence (ECL) biosensor for microRNA-21 (miRNA-21) detection. The novel Luminol-based signal tags was synthesized utilizing the π-π stacking interaction between PTCA and Luminol, realizing highly effective and stable immobilization of Luminol and resulting in good stability and strong ECL response. Meanwhile, target miRNA-21 triggered disaggregation of cruciform DNA structure was used to mediate exponential SDR for target recycling amplification. Taking advantage of the novel Luminol-based signal tag and exponential SDR, the proposed ECL biosensor achieved excellent sensitivity with wide linear range from 10 aM to 100 pM and detection limit was 2 aM. Moreover, this ECL biosensor was applied to estimate the expression level of miRNA-21 and pharmacodynamics of matrine in human breast cancer cells (MCF-7 cells). The proposed biosensor provided a new opportunity for the preparation of ECL nanomaterials and exhibited great application potential in other biomarkers detection, clinical application and pharmacodynamics evaluation.
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an ultrasensitive electrochemiluminescence immunosensor for nt probnp based on self catalyzed luminescence emitter coupled with pdcu carbon nanohorn hybrid
Biosensors and Bioelectronics, 2017Co-Authors: Haijun Wang, Chengyi Xiong, Yaqin Chai, Ruo YuanAbstract:Abstract A novel electrochemiluminescence (ECL) signal amplified strategy based on self-catalyzed Luminol derivative and carbon nanohorn-based hybrid for Luminol/H 2 O 2 system was firstly proposed, and applied to fabricate an ultrasensitive ECL immunosensor for the detection of N-terminal pro-brain natriuretic peptides (NT-proBNP). Initially, PdCu nanocubes supported single-walled carbon nanohorns (PdCu@SWCNHs) hybrid was successfully synthesized through a facile one-pot strategy. For signal tag fabrication, 3,4,9,10-perylenetetracarboxylic acid (PTCA) conjugated Luminol (PTC-Lu) as a novel self-catalyzed luminescence emitter was prepared and then effectively immobilized on the PdCu@SWCNHs to form PTC-Lu/PdCu@SWCNHs nanocomposite via π-π stacking. Herein, PTCA was selected and introduced into the Luminol/H 2 O 2 ECL system for the first time, which could not only increase the luminescence signal but also remarkably improve the stability and hydrophilicity of pristine SWCNHs. More notably, PdCu@SWCNHs nanohybrid showed superior catalytic activity toward H 2 O 2 that could further amplify the ECL signal of Luminol/H 2 O 2 system. Meanwhile, good biocompatibility and high specific surface area of PdCu nanocubes make the composite possess excellent property for immobilization of detection antibody. Based upon above, the proposed “signal on” immunosensor exhibited a wide linear detection range of 0.1 pg mL −1 to 25 ng mL −1 with a relatively low detection limit of 0.05 pg mL −1 , and successfully achieved the detection in clinical human serum samples with desirable results.
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ceria doped zinc oxide nanoflowers enhanced Luminol based electrochemiluminescence immunosensor for amyloid β detection
ACS Applied Materials & Interfaces, 2016Co-Authors: Jingxi Wang, Ruo Yuan, Haijun Wang, Ying Zhou, Ying Zhuo, Yaqin ChaiAbstract:In this work, ceria doped ZnO nanomaterials with flower-structure (Ce:ZONFs) were prepared to construct a Luminol-based electrochemiluminescence (ECL) immunosensor for amyloid-β protein (Aβ) detection. Herein, carboxyl groups (−COOH) covered Ce:ZONFs were synthesized by a green method with lysine as reductant. After that, Ce:ZONFs-based ECL nanocomposite was prepared by combining the luminophore of Luminol and Ce:ZONFs via amidation and physical absorption. Luminol modified on Ce:ZONFs surface could generate a strong ECL signal under the assistance of reactive oxygen species (ROSs) (such as OH• and O2•–), which were produced by a catalytic reaction between Ce:ZONFs and H2O2. It was worth noticing that a quick Ce4+ ↔ Ce3+ reaction in this doped material could increase the rate of electron transfer to realize the signal amplification. Subsequently, the Luminol functionalized Ce:ZONFs (Ce:ZONFs-Lum) were covered by secondary antibody (Ab2) and glucose oxidase (GOD), respectively, to construct a novel Ab2 bio...
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electrochemiluminescence immunosensor based on multifunctional Luminol capped aunps fe3o4 nanocomposite for the detection of mucin 1
Biosensors and Bioelectronics, 2015Co-Authors: Jingxi Wang, Ruo Yuan, Ying Zhou, Ying Zhuo, Yaqin ChaiAbstract:In this work, a novel and multifunctional nanocomposite of Luminol capped gold modified Fe3O4 (Lu-AuNPs@Fe3O4) was utilized as the carrier of secondary antibody (Ab2) to fabricate a sandwiched electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of mucin-1 (MUC1). Herein, the Luminol capped gold nanoparticles (Lu-AuNPs) were synthesized with HAuCl4 and Luminol by the help of NaBH4 at room temperature, and then Lu-AuNPs were adsorbed on the Fe3O4 magnetic nanoparticles (MNPs) to form the nanocomposite of Lu-AuNPs@Fe3O4 via electrostatic interaction. Fe3O4 MNPs in Lu-AuNPs@Fe3O4 exhibited excellent conductivity and admirable catalytic activity in H2O2 decomposition, which could enhance the ECL efficiency of Luminol-H2O2 system. In addition, the substrates of gold coated ZnO nanoparticles (AuNPs@ZnO), providing large specific surface areas for primary antibody (Ab1) capturing, were modified on the electrode. As a result, a wide linear range of 7 orders of magnitude from 10 fg/mL to 10 ng/mL was obtained with an ultralow detection limit of 4.5 fg/mL for MUC1.
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sensitive electrochemiluminescence detection for ca15 3 based on immobilizing Luminol on dendrimer functionalized zno nanorods
Biosensors and Bioelectronics, 2015Co-Authors: Xinya Jiang, Ruo Yuan, Haijun Wang, Yaqin ChaiAbstract:Abstract In this study, we constructed a novel electrochemiluminescence (ECL) immunosensor for sensitive and selective detection of carbohydrate antigen 15-3 (CA15-3) by using polyamidoamine (PAMAM)-functionalized ZnO nanorods (ZNs-PAMAM) as carriers. PAMAM dendrimers with hyper-branched and three-dimensional structure were used as linked reagents for co-immobilization of Luminol and CA15-3 detection antibody on the ZNs to prepare the signal probe. In addition, ZNs could hasten the decomposition of H2O2 to generate various reactive oxygen species (ROSs) which accelerated the ECL reaction of Luminol with amplified ECL intensity. Compared with Luminol in the detection solution, the ECL efficiencies of Luminol could be improved by immobilizing Luminol on the electrode due to the smaller distance between luminescence reagent and the electrode surface. Moreover, the electrodepositing gold nanoparticles (AuNPs) on the bare glass carbon electrode (GCE) with enhanced surface area could capture a large amount of primary anti-CA15-3 to improve the sensitivity of the immunosensor. Under the optimized experimental conditions, a wide linear range of 0.1–120 U mL−1 was acquired with a relatively low detection limit of 0.033 U mL−1 (S/N=3) for CA15-3.
