The Experts below are selected from a list of 1026 Experts worldwide ranked by ideXlab platform
Ivan J Maureirabutler - One of the best experts on this subject based on the ideXlab platform.
-
development and characterization of indel markers for Lupinus luteus l fabaceae and cross species amplification in other lupin species
Electronic Journal of Biotechnology, 2018Co-Authors: Claudia E Osorio, Joshua A Udall, Haroldo Salvogarrido, Ivan J MaureirabutlerAbstract:Background: Strong artificial selection and/or natural bottle necks may limit genetic variation in domesticated species. Lupinus luteus, an orphan temperate crop, has suffered diversity reductions during its bitter/sweet alkaloid domestication history, limiting breeding efforts and making molecular marker development a difficult task. The main goal of this research was to generate new polymorphic insertion-deletion (InDel) markers to aid yellow lupin genetics and breeding. By combining genomic reduction libraries and next generation sequencing, several polymorphic InDel markers were developed for L. luteus L. Results: A total of 118 InDel in silico polymorphic markers were identified. Eighteen InDel primer sets were evaluated in a diverse L. luteus core collection, where amplified between 2-3 alleles per locus. Observed heterozygosity (HO; 0.0648 to 0.5564) and polymorphic information content (PIC; 0.06 to 0.48) estimations revealed a moderate level of genetic variation across L. luteus accessions. In addition, ten and nine InDel loci amplified successfully Lupinus hispanicus Boiss & Reut, and Lupinus mutabilis Sweet, respectively, two L. luteus close relatives. PCA analysis identified two L. luteus clusters, most likely explained by the domestication species history. Conclusion: The development of InDel markers will facilitate the study of genetic diversity across L. luteus populations, as well as among closely related species.
-
chromatographic fingerprinting of Lupinus luteus l leguminosae main secondary metabolites a case of domestication affecting crop variability
Genetic Resources and Crop Evolution, 2018Co-Authors: Claudia E Osorio, Jeff J. Doyle, Joshua A Udall, Veronique S E Amiard, Javiera Aravenacalvo, Ivan J MaureirabutlerAbstract:Secondary metabolites (SMs), such as alkaloids and raffinose oligosaccharides (RFOs), play important roles in plant physiology. Although alkaloid and RFO phenotypic variation has been reported for yellow lupin (Lupinus luteus L.), most evaluations have used a reduced number of accessions; thus, limiting the understanding of accumulation patterns and variation ranges. The main goal of this research was to assess alkaloid and RFO content in a diverse L. luteus sample to understand possible SM accumulation patterns across this legume species. Eighteen yellow lupin accessions were analyzed using high performance thin layer chromatography to provide insights on seed and leaf RFO and alkaloid phenotypic variation. Co-dominant markers (170) were used to examine genetic relationships among L. luteus accessions and possible accumulation patterns across closely related genotypes. Significant differences were observed for seed and leaf RFOs. Total seed RFO accumulation ranged from 79.738 to 131.079 mg g−1. Raffinose, stachyose, and verbascose were observed in all genotypes’ seeds, but at different RFO concentrations. Raffinose was the only RFO detected in leaves (2.793–0.4224 mg g−1). Total alkaloid accumulation ranged from 0.22 to 15.12 and 0.00 to 8.007 mg g−1 for seeds and leaves, respectively. Lupinine, sparteine, and gramine were observed in seeds and leaves, and showed a wide range of variation. Neighbor-Joining (NJ) analysis showed an apparent pattern of seed alkaloid accumulation, most likely due to domestication events. However, high RFO accumulating accessions were scattered across the NJ tree. Alkaloid and RFO significant phenotypic variation will not only help to understand the roles of these SMs in L. luteus, but also to uncover the genetic basis behind their accumulation.
-
functional and physicochemical properties of a protein isolate from aluprot cgna a novel protein rich lupin variety Lupinus luteus
Food Research International, 2015Co-Authors: Jose A Piornos, Ivan J Maureirabutler, Takahiro Ogura, Cesar Burgosdiaz, Eduardo Morales, Monica Rubilar, Haroldo SalvogarridoAbstract:Abstract This study describes the isolation of proteins from the novel lupin variety Alu Prot -CGNA ( Lupinus luteus ) and the influence of pH and NaCl on their functional properties. Alu Prot -CGNA variety showed to have a great protein content in dehulled seeds (60.60 g protein/100 g, dry matter), which is higher than soybean and other lupin varieties. A lupin protein isolate (97.54 g protein/100 g) from Alu Prot -CGNA, LPIA, was prepared from lupin flour by alkali solubilization and isoelectric precipitation. The solubility profile of the LPIA was affected by pH, where the minimal values were observed at pH values close to its isoelectric point range (pH 4–5). The highest values of water absorption capacity (1.71 cm 3 H 2 O/g protein), oil absorption capacity (1.43 g trapped oil/g protein), emulsifying capacity (61.94%), emulsion stability (96.43%), foaming capacity (114.29%), foam stability (65.69%) and least gelation concentration (20 g/100 cm 3 ) were observed at pH values lower and higher than its isoelectric point. In the presence of 100 mM of NaCl, their functional properties were improved. SDS-PAGE showed that LPIA mainly contained high molecular weight proteins (α and β-conglutin). These results are useful for increasing the utilization of this protein isolate as a potential functional ingredient in food industry.
-
proteomic characterization of seeds from yellow lupin Lupinus luteus l
Proteomics, 2014Co-Authors: Takahiro Ogura, Ivan J Maureirabutler, Jun Ogihara, Michio Sunairi, Hidetaka Takeishi, Tomoko Aizawa, Marcos R Olivostrujillo, Haroldo SalvogarridoAbstract:Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).
-
yellow lupin Lupinus luteus l transcriptome sequencing molecular marker development and comparative studies
BMC Genomics, 2012Co-Authors: Lorena B Parragonzalez, Gabriela A Aravenaabarzua, Cristell S Navarronavarro, Joshua A Udall, Jeff Maughan, Louis M Peterson, Haroldo Salvogarrido, Ivan J MaureirabutlerAbstract:Background Yellow lupin (Lupinus luteus L.) is a minor legume crop characterized by its high seed protein content. Although grown in several temperate countries, its orphan condition has limited the generation of genomic tools to aid breeding efforts to improve yield and nutritional quality. In this study, we report the construction of 454-expresed sequence tag (EST) libraries, carried out comparative studies between L. luteus and model legume species, developed a comprehensive set of EST-simple sequence repeat (SSR) markers, and validated their utility on diversity studies and transferability to related species.
Mariusz Olczak - One of the best experts on this subject based on the ideXlab platform.
-
diphosphonucleotide phosphatase phosphodiesterase from yellow lupin Lupinus luteus l belongs to a novel group of specific metallophosphatases
FEBS Letters, 2002Co-Authors: Mariusz Olczak, Teresa OlczakAbstract:A cDNA encoding previously purified and characterized diphosphonucleotide phosphatase/phosphodiesterase (PPD1) from yellow lupin (Lupinus luteus L.) was identified. The ppd1 gene encodes a protein containing a cleavable signal sequence. A functional expression of PPD1 in Saccharomyces cerevisiae confirmed the proper gene identification. A gene homologous to ppd1, encoding a putative membrane protein (PPD2), as well as fragments of two other genes encoding PPD3 and PPD4 proteins were also isolated. Amino acids composing the putative active center of PPD1 and PPD2 are similar to those present in known purple acid phosphatases, which suggests that the reported genes might encode a novel group of specific metallophosphatases. RT-PCR revealed that the corresponding PPD1 mRNA accumulates in stems and leaves, and PPD2 mRNA in stems, leaves and seedlings.
-
characterization of diphosphonucleotide phosphatase phosphodiesterase from yellow lupin Lupinus luteus seeds
Biochimica et Biophysica Acta, 2000Co-Authors: Mariusz Olczak, Marcin Kobialka, Wieslaw WatorekAbstract:A phosphatase cleaving the pyrophosphate bond in diphosphonucleotides and phosphodiester bond in various phosphodiesters (pH optimum at 6.25) was purified from yellow lupin (Lupinus luteus L.) seeds. The enzyme is 75 kDa monomeric glycoprotein (pI=6.4) with 4.4% of carbohydrate (mannose, N-acetylglucosamine, fucose and xylose). Analysis of its partial amino acid sequence (8 peptides, 101 amino acid residues) together with no divalent cation requirements for catalysis points out that the purified enzyme is different from known plant pyrophosphate cleaving enzymes (apyrases and inorganic pyrophosphatases). Its physiological role could be related to a regulation of diphosphonucleotides level in plant metabolism.
-
purification and characterization of acid phosphatase from yellow lupin Lupinus luteus seeds
Biochimica et Biophysica Acta, 1997Co-Authors: Mariusz Olczak, Wieslaw Watorek, Bronislawa MorawieckaAbstract:Acid phosphatase (EC 3.1.3.2) from yellow lupin (Lupinus luteus) seeds was purified to homogeneity by ammonium sulphate fractionation, affinity chromatography, cation-exchange chromatography, gel filtration or reverse-phase HPLC. The enzyme is a dimer with the 50 kD and 44 kD subunits and contains 7.3% of carbohydrate, forming at least four oligosaccharide chains. The optimum pH for the enzyme is 5.4. The apparent Km for p-nitrophenyl phosphate was estimated to be 0.28 mM and Vmax = 1780 IU/mg of protein. The purified phosphatase has the highest specific activities reported for any plant acid phosphatases measured for any native or synthetic substrate. The enzyme has broad specificity; however, cyclic nucleotides, pyrophosphate or phytate are not cleaved. It is inhibited by molybdate, fluoride and phosphate. There is no change in the enzyme activity in the presence of EDTA, phenanthroline and tartrate.
Haroldo Salvogarrido - One of the best experts on this subject based on the ideXlab platform.
-
development and characterization of indel markers for Lupinus luteus l fabaceae and cross species amplification in other lupin species
Electronic Journal of Biotechnology, 2018Co-Authors: Claudia E Osorio, Joshua A Udall, Haroldo Salvogarrido, Ivan J MaureirabutlerAbstract:Background: Strong artificial selection and/or natural bottle necks may limit genetic variation in domesticated species. Lupinus luteus, an orphan temperate crop, has suffered diversity reductions during its bitter/sweet alkaloid domestication history, limiting breeding efforts and making molecular marker development a difficult task. The main goal of this research was to generate new polymorphic insertion-deletion (InDel) markers to aid yellow lupin genetics and breeding. By combining genomic reduction libraries and next generation sequencing, several polymorphic InDel markers were developed for L. luteus L. Results: A total of 118 InDel in silico polymorphic markers were identified. Eighteen InDel primer sets were evaluated in a diverse L. luteus core collection, where amplified between 2-3 alleles per locus. Observed heterozygosity (HO; 0.0648 to 0.5564) and polymorphic information content (PIC; 0.06 to 0.48) estimations revealed a moderate level of genetic variation across L. luteus accessions. In addition, ten and nine InDel loci amplified successfully Lupinus hispanicus Boiss & Reut, and Lupinus mutabilis Sweet, respectively, two L. luteus close relatives. PCA analysis identified two L. luteus clusters, most likely explained by the domestication species history. Conclusion: The development of InDel markers will facilitate the study of genetic diversity across L. luteus populations, as well as among closely related species.
-
functional and physicochemical properties of a protein isolate from aluprot cgna a novel protein rich lupin variety Lupinus luteus
Food Research International, 2015Co-Authors: Jose A Piornos, Ivan J Maureirabutler, Takahiro Ogura, Cesar Burgosdiaz, Eduardo Morales, Monica Rubilar, Haroldo SalvogarridoAbstract:Abstract This study describes the isolation of proteins from the novel lupin variety Alu Prot -CGNA ( Lupinus luteus ) and the influence of pH and NaCl on their functional properties. Alu Prot -CGNA variety showed to have a great protein content in dehulled seeds (60.60 g protein/100 g, dry matter), which is higher than soybean and other lupin varieties. A lupin protein isolate (97.54 g protein/100 g) from Alu Prot -CGNA, LPIA, was prepared from lupin flour by alkali solubilization and isoelectric precipitation. The solubility profile of the LPIA was affected by pH, where the minimal values were observed at pH values close to its isoelectric point range (pH 4–5). The highest values of water absorption capacity (1.71 cm 3 H 2 O/g protein), oil absorption capacity (1.43 g trapped oil/g protein), emulsifying capacity (61.94%), emulsion stability (96.43%), foaming capacity (114.29%), foam stability (65.69%) and least gelation concentration (20 g/100 cm 3 ) were observed at pH values lower and higher than its isoelectric point. In the presence of 100 mM of NaCl, their functional properties were improved. SDS-PAGE showed that LPIA mainly contained high molecular weight proteins (α and β-conglutin). These results are useful for increasing the utilization of this protein isolate as a potential functional ingredient in food industry.
-
proteomic characterization of seeds from yellow lupin Lupinus luteus l
Proteomics, 2014Co-Authors: Takahiro Ogura, Ivan J Maureirabutler, Jun Ogihara, Michio Sunairi, Hidetaka Takeishi, Tomoko Aizawa, Marcos R Olivostrujillo, Haroldo SalvogarridoAbstract:Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).
-
yellow lupin Lupinus luteus l transcriptome sequencing molecular marker development and comparative studies
BMC Genomics, 2012Co-Authors: Lorena B Parragonzalez, Gabriela A Aravenaabarzua, Cristell S Navarronavarro, Joshua A Udall, Jeff Maughan, Louis M Peterson, Haroldo Salvogarrido, Ivan J MaureirabutlerAbstract:Background Yellow lupin (Lupinus luteus L.) is a minor legume crop characterized by its high seed protein content. Although grown in several temperate countries, its orphan condition has limited the generation of genomic tools to aid breeding efforts to improve yield and nutritional quality. In this study, we report the construction of 454-expresed sequence tag (EST) libraries, carried out comparative studies between L. luteus and model legume species, developed a comprehensive set of EST-simple sequence repeat (SSR) markers, and validated their utility on diversity studies and transferability to related species.
-
yellow lupin Lupinus luteus l transcriptome sequencing molecular marker development and comparative studies
BMC Genomics, 2012Co-Authors: Lorena B Parragonzalez, Gabriela A Aravenaabarzua, Cristell S Navarronavarro, Joshua A Udall, Jeff Maughan, Louis M Peterson, Haroldo Salvogarrido, Ivan J MaureirabutlerAbstract:Yellow lupin (Lupinus luteus L.) is a minor legume crop characterized by its high seed protein content. Although grown in several temperate countries, its orphan condition has limited the generation of genomic tools to aid breeding efforts to improve yield and nutritional quality. In this study, we report the construction of 454-expresed sequence tag (EST) libraries, carried out comparative studies between L. luteus and model legume species, developed a comprehensive set of EST-simple sequence repeat (SSR) markers, and validated their utility on diversity studies and transferability to related species. Two runs of 454 pyrosequencing yielded 205 Mb and 530 Mb of sequence data for L1 (young leaves, buds and flowers) and L2 (immature seeds) EST- libraries. A combined assembly (L1L2) yielded 71,655 contigs with an average contig length of 632 nucleotides. L1L2 contigs were clustered into 55,309 isotigs. 38,200 isotigs translated into proteins and 8,741 of them were full length. Around 57% of L. luteus sequences had significant similarity with at least one sequence of Medicago, Lotus, Arabidopsis, or Glycine, and 40.17% showed positive matches with all of these species. L. luteus isotigs were also screened for the presence of SSR sequences. A total of 2,572 isotigs contained at least one EST-SSR, with a frequency of one SSR per 17.75 kbp. Empirical evaluation of the EST-SSR candidate markers resulted in 222 polymorphic EST-SSRs. Two hundred and fifty four (65.7%) and 113 (30%) SSR primer pairs were able to amplify fragments from L. hispanicus and L. mutabilis DNA, respectively. Fifty polymorphic EST-SSRs were used to genotype a sample of 64 L. luteus accessions. Neighbor-joining distance analysis detected the existence of several clusters among L. luteus accessions, strongly suggesting the existence of population subdivisions. However, no clear clustering patterns followed the accession’s origin. L. luteus deep transcriptome sequencing will facilitate the further development of genomic tools and lupin germplasm. Massive sequencing of cDNA libraries will continue to produce raw materials for gene discovery, identification of polymorphisms (SNPs, EST-SSRs, INDELs, etc.) for marker development, anchoring sequences for genome comparisons and putative gene candidates for QTL detection.
Lech Ratajczak - One of the best experts on this subject based on the ideXlab platform.
-
lipid and protein accumulation in developing seeds of three lupine species Lupinus luteus l Lupinus albus l and Lupinus mutabilis sweet
Journal of Experimental Botany, 2009Co-Authors: Slawomir Borek, Stanislawa Pukacka, Krzysztof Michalski, Lech RatajczakAbstract:A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7-14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from developing seeds were isolated and cultured in vitro for 96 h on Heller medium with 60 mM sucrose (+S) or without sucrose (-S). Each medium was additionally enriched with 35 mM asparagine or 35 mM NaNO 3 . Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid. Experiments with [1- 14 C]acetate and [2- 14 C]acetate showed that the decrease in lipid accumulation in developing lupine seeds resulted from exhaustion of lipid precursors rather than from degradation or modification of the enzymatic apparatus. The carbon atom from the C-1 position of acetate was liberated mainly as CO 2 , whereas the carbon atom from the C-2 position was preferentially used in anabolic pathways. The dominant phospholipid in the investigated lupine seed storage organs was phosphatidylcholine. The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids. The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.
-
Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet
Journal of experimental botany, 2009Co-Authors: Sławomir Borek, Stanislawa Pukacka, Krzysztof Michalski, Lech RatajczakAbstract:A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7-14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from developing seeds were isolated and cultured in vitro for 96 h on Heller medium with 60 mM sucrose (+S) or without sucrose (-S). Each medium was additionally enriched with 35 mM asparagine or 35 mM NaNO3. Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid. Experiments with [1-14C]acetate and [2-14C]acetate showed that the decrease in lipid accumulation in developing lupine seeds resulted from exhaustion of lipid precursors rather than from degradation or modification of the enzymatic apparatus. The carbon atom from the C-1 position of acetate was liberated mainly as CO2, whereas the carbon atom from the C-2 position was preferentially used in anabolic pathways. The dominant phospholipid in the investigated lupine seed storage organs was phosphatidylcholine. The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids. The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.
Edison Serrano - One of the best experts on this subject based on the ideXlab platform.
-
responses in rainbow trout oncorhynchus mykiss to increasing dietary doses of lupinine the main quinolizidine alkaloid found in yellow lupins Lupinus luteus
Aquaculture, 2011Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Margareth Overland, Jon Ovrum Hansen, Liv Torunn MydlandAbstract:Abstract This experiment investigated the effect of increasing dietary doses of lupinine, the main quinolizidine alkaloid in Lupinus luteus , on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout ( Oncorhynchus mykiss ). Duplicate groups of rainbow trout (initial body weight of 330 g) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine kg −1 for 60 days. Feed intake and growth were reduced in response to dietary lupinine, best fit by quadratic regression. Based on these results, the practical tolerance level of lupinine, with regard to growth and feed intake, was ≤ 100 mg kg −1 feed. Carcass composition did not vary among treatments. Despite a depletion of glycogen and lipid stores in the hepatocytes, lupinine did not induce any morphological changes in spleen, kidney, heart or intestinal tissues. These results indicate that the lupinine possesses a strong anti-palatability effect, but does not appear to pose short-term health risks for rainbow trout.
-
responses in rainbow trout oncorhynchus mykiss to increasing dietary dose of lupinine alkaloid
Lupins for health and wealth. Proceedings of the 12th International Lupin Conference Fremantle Western Australia 14-18 September 2008, 2008Co-Authors: Edison Serrano, Trond Storebakken, Michael H Penn, Jon Ovrum Hansen, Thor Landsverk, Liv Torunn MydlandAbstract:Yellow lupin ( Lupinus luteus ) is a promising source of protein in feeds for carnivorous fish. However, the high content of alkaloids may limit its potential for use. Lupinine is the main quinolizidine alkaloid in several varieties of Lupinus luteus . It has been reported to be highly toxic for bacteria and invertebrates, however no information is available about the allelopathic effects of lupinine on vertebrates. This study investigated the effect of increasing dietary doses of lupinine on feed intake, growth performance, tissue histology and nutritional composition of rainbow trout. Duplicate groups of rainbow trout (initial body weight of 0.3 kg) were fed extruded fish meal based diets containing 0, 50, 75, 100, 250, 500, 1000 and 5000 mg lupinine/kg for 60 days. Results from this study show that feed intake and growth were linearly reduced in response to dietary lupinine. Based on results obtained by additional analysis of variance, the practical tolerance levels of lupinine with regard to growth and feed intake was up to 100 mg/kg feed. Carcass composition did not vary among treatment. Besides a depletion of glycogen and lipid stores in the hepatocytes, the dietary inclusion of lupinine did not cause any morphological changes in kidney, heart or intestinal tissue. These results indicate that the lupinine alkaloid possesses a strong anti-palatability effect, but does not pose an adverse short-term risk to the health of rainbow trout.
