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Reginaldo Peçanha Brazil - One of the best experts on this subject based on the ideXlab platform.
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Lutzomyia longipalpis Breeding—A Probable Breeding Substrate for Lutzomyia longipalpis in Nature
Open Journal of Animal Sciences, 2018Co-Authors: João Carlos Araujo Carreira, Reginaldo Peçanha Brazil, Bianca Dos Santos Carvalho, Alba Valéria Machado Da SilvaAbstract:The identifying of the preferred breeding sites of sand fly immature forms is quite important to the understanding of the vector’s biology and the development of more effective control strategies for leishmaniasis. In the present study, it was analyzed experimentally; the potential of dog feces of serving as breeding substrate to Lutzomyia longipalpis. Two situations were tested: 1) the life cycle of the insects from larvae to adults, 2) the whole cycle from adults to adults. Our results demonstrated for the first time that: 1) L. longipalpis can be easily bred in dog feces, suggesting that it could represent a potential raising substrate to immature forms for this species; 2) L. longipalpis larvae are able to make galleries on a substrate, literally burying themselves in this to function also as a shelter; 3) from the previous information that dogs infected with Ancylostoma caninum can present dodecanoic acid in their feces, we suggested that this substrate may be particularly more attractive to L. longipalpis females seen that this molecule is the same component of their oviposition pheromone, which is produced by the accessory glands and secreted onto the eggs; 4) after the copulation the males may landing on the substrate and continued beating the wings intermittently until the death in a similar behavior also noticed in other situations associated to kairomones response or pheromone production. Therefore, we suggested that it is very likely that dog excrements could serve as an important breeding substrate for L. longipalpis in endemic areas of visceral leishmaniasis in the New World.
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the current status of the Lutzomyia longipalpis diptera psychodidae phlebotominae species complex
Memorias Do Instituto Oswaldo Cruz, 2017Co-Authors: N.a. De Souza, Reginaldo Peçanha Brazil, Alejandra S. ArakiAbstract:Lutzomyia longipalpis s.l. is a complex of sibling species and is the principal vector of American visceral leishmaniasis. The present review summarises the diversity of efforts that have been undertaken to elucidate the number of unnamed species in this species complex and the phylogenetic relationships among them. A wide variety of evidence, including chemical, behavioral and molecular traits, suggests very recent speciation events and complex population structure in this group. Although significant advances have been achieved to date, differential vector capacity and the correlation between structure of parasite and vector populations have yet to be elucidated. Furthermore, increased knowledge about recent epidemiological changes, such as urbanisation, is essential for pursuing effective strategies for sandfly control in the New World.
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transcriptome exploration of the sex pheromone gland of Lutzomyia longipalpis diptera psychodidae phlebotominae
Parasites & Vectors, 2013Co-Authors: Natalia Gonzalezcaballero, Jose M C Ribeiro, Jesus G Valenzuela, Patricia Cuervo, Reginaldo Peçanha BrazilAbstract:Background Molecules involved in pheromone biosynthesis may represent alternative targets for insect population control. This may be particularly useful in managing the reproduction of Lutzomyia longipalpis, the main vector of the protozoan parasite Leishmania infantum in Latin America. Besides the chemical identity of the major components of the L. longipalpis sex pheromone, there is no information regarding the molecular biology behind its production. To understand this process, obtaining information on which genes are expressed in the pheromone gland is essential.
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Spatial dynamics of urban populations of Lutzomyia longipalpis (Diptera: Psychodidae) in Caxias, State of Maranhão, Brazil
Revista da Sociedade Brasileira de Medicina Tropical, 2013Co-Authors: Maria Do Desterro Soares Brandão Nascimento, José Manuel Macário Rebêlo, Maria Helena Silva, Graça Maria De Castro Viana, Francisco Santos Leonardo, Geusa Felipa De Barros Bezerra, Antônia Suely Guimarães E Silva, Valeria Cristina Pinheiro Soares, Silma Regina Ferreira Pereira, Reginaldo Peçanha BrazilAbstract:Introduction In this paper, we report the ecology of Lutzomyia longipalpis in Caxias City, located in the eastern part of State of Maranhao, Brazil and highlight its seasonal and geographical distribution by environment. In addition, we discuss natural Leishmania infection and its relationship with visceral leishmaniasis. Methods Between September 2007 and August 2009, the collection of sandflies was performed using Center for Disease Control (CDC) light traps from 15 houses in 5 selected neighborhoods. Results Lutzomyia longipalpis was present in all zones of the city. We also found that Lu. longipalpis was regularly detected both inside and around the house, predominantly in outdoor areas. In urban areas, Lu. longipalpis was present in both the dry and rainy seasons, with a higher density present in the latter. One female specimen of Lu. longipalpis was observed to have natural Leishmania infection. Conclusions The presence of Lu. longipalpis was observed throughout the year during 2 seasonal periods, with a predominance in the rainy season. A low rate of natural Leishmania infection was observed in urban areas during the rainy season.
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Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) in the region of Saquarema: potential area of visceral leishmaniasis transmission in the State of Rio de Janeiro, Brazil
Revista da Sociedade Brasileira de Medicina Tropical, 2012Co-Authors: Reginaldo Peçanha Brazil, Michelle Cristina De Queiroz Pontes, Wagner Lança Passos, Andressa Alencaste Fuzari, Beatriz Gomes BrazilAbstract:Introduction: Lutzomyia longipalpis is the main vector of Leishmania infantum chagasi in the Americas. Methods: Phlebotomine captures were conducted during 2008 and 2009 in a rural area endemic for cutaneous leishmaniasis located in the municipality of Saquarema, Rio de Janeiro. Results: Among other species captured, we observed the presence of Lutzomyia longipalpis. Conclusions: This is the first report of the occurrence of Lutzomyia longipalpis in this region, demonstrating the potential risk of visceral leishmaniasis transmission in the coastal area of the State of Rio de Janeiro. Therefore, active vigilance by all municipalities in the area is necessary.
Alexandre A. Peixoto - One of the best experts on this subject based on the ideXlab platform.
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Correction: Fine Structure of the Male Reproductive System and Reproductive Behavior of Lutzomyia longipalpis Sandflies (Diptera: Psychodidae: Phlebotominae)
PLoS ONE, 2014Co-Authors: Carolina N. Spiegel, Alexandre A. Peixoto, Jorge A. C. Bretas, Felipe M. Vigoder, Rafaela Vieira Bruno, Maurilio J. SoaresAbstract:There is an error in Figure 6A. The genitalia in Figure 6A belongs to another species (Evandromyia lenti), and not to Lutzomyia longipalpis as described in the legend. Please see the corrected Figure 6A of Lutzomyia longipalpis here:
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Sex pheromone and period gene characterization of Lutzomyia longipalpis sensu lato (Lutz & Neiva) (Diptera: Psychodidae) from Posadas, Argentina
Memorias do Instituto Oswaldo Cruz, 2010Co-Authors: Oscar Daniel Salomon, James G. C. Hamilton, Alejandra S. Araki, Soraya Alejandra Acardi, Alexandre A. PeixotoAbstract:Lutzomyia longipalpis s.l. is the primary vector of Leishmania (L.) infantum in the New World. In this study, male Lutzomyia longipalpis specimens from Posadas, Argentina were characterized for two polymorphic markers: the male sex pheromone and the period (per) gene. The male sex pheromone was identified as (S)-9-methylgermacrene-B, the same compound produced by Lu. longipalpis from Paraguay and many populations from Brazil. The analysis of per gene sequences revealed that the population from Argentina is significantly differentiated from previously studied Brazilian populations. Marker studies could contribute to the understanding of the distribution and spread of urban American visceral leishmaniasis, thus aiding in the design of regional surveillance and control strategies.
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the Lutzomyia longipalpis species complex does population sub structure matter to leishmania transmission
Trends in Parasitology, 2008Co-Authors: R. Maingon, R. D. Ward, J G C Hamilton, Luiz Guilherme Soares Da Rocha Bauzer, Alexandre A. PeixotoAbstract:Leishmania chagasi causes visceral leishmaniasis and, to a lesser extent, atypical cutaneous leishmaniasis in Central and South America. Its main sand fly vector, Lutzomyia longipalpis s.l. (Diptera: Psychodidae) displays a complex population structure that might contribute to the observed clinical pleomorphism and to recent major urban epidemics. This article summarises our understanding on reproductive barriers and hybridisation among this vector's sibling incipient species. Identifying genes important for sand fly ecological adaptability and sand fly– Leishmania genetic co-variation could be helpful for interrupting Leishmania transmission.
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Lutzomyia longipalpis in brazil a complex or a single species a mini review
Memorias Do Instituto Oswaldo Cruz, 2007Co-Authors: Luiz Guilherme Soares Da Rocha Bauzer, N.a. De Souza, R. Maingon, Alexandre A. PeixotoAbstract:Lutzomyia longipalpis is the main vector of Leishmania infantum chagasi, the causative agent of American visceral leishmaniasis (AVL). Although there is strong evidence that Lu. longipalpis is a species complex, not all data concerning populations from Brazil support this hypothesis. The issue is still somewhat controversial for this large part of Lu. longipalpis distribution range even though that it is the Latin American region contributing to most of the cases of AVL. In this mini-review we consider in detail the current data for the Brazilian populations and conclude that Lu. longipalpis is a complex of incipient vector species with a complexity similar to Anopheles gambiae s.s. in Africa.
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Copulation songs in three siblings of Lutzomyia longipalpis (Diptera: Psychodidae)
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2004Co-Authors: N.a. De Souza, Charalambos P. Kyriacou, Richard D. Ward, James G. C. Hamilton, Alexandre A. PeixotoAbstract:We present the results of recording male courtship songs of the sandfly Lutzomyia longipalpis. The striking differences in the songs from 3 Brazilian populations of this sandfly with 3 distinct male pheromones support the 3 sibling species previously proposed based on this characteristic.
James G. C. Hamilton - One of the best experts on this subject based on the ideXlab platform.
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Sex pheromone and period gene characterization of Lutzomyia longipalpis sensu lato (Lutz & Neiva) (Diptera: Psychodidae) from Posadas, Argentina
Memorias do Instituto Oswaldo Cruz, 2010Co-Authors: Oscar Daniel Salomon, James G. C. Hamilton, Alejandra S. Araki, Soraya Alejandra Acardi, Alexandre A. PeixotoAbstract:Lutzomyia longipalpis s.l. is the primary vector of Leishmania (L.) infantum in the New World. In this study, male Lutzomyia longipalpis specimens from Posadas, Argentina were characterized for two polymorphic markers: the male sex pheromone and the period (per) gene. The male sex pheromone was identified as (S)-9-methylgermacrene-B, the same compound produced by Lu. longipalpis from Paraguay and many populations from Brazil. The analysis of per gene sequences revealed that the population from Argentina is significantly differentiated from previously studied Brazilian populations. Marker studies could contribute to the understanding of the distribution and spread of urban American visceral leishmaniasis, thus aiding in the design of regional surveillance and control strategies.
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Identification of the sex pheromone of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) from Asunción, Paraguay
Parasites & Vectors, 2009Co-Authors: Reginaldo Peçanha Brazil, Norath Natalia Caballero, James G. C. HamiltonAbstract:The sand fly Lutzomyia longipalpis is the main vector of Leishmania (L.) infantum (Nicolle), the causative agent of American visceral leishmaniasis (AVL) in the New World. Male Lu. longipalpis have secretory glands which produce sex pheromones in either abdominal tergites 4 or 3 and 4. These glands are sites of sex pheromone production and each pheromone type may represent true sibling species. In Latin America, apart from Lu. pseudolongipalpis Arrivillaga and Feliciangeli from Venezuela, populations of Lu. longipalpis s.l. can be identified by their male-produced sex pheromones: (S)-9-methylgermacrene-B, 3-methyl-α-himachalene and the two cembrenes, 1 and 2.
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Male sex pheromones and the phylogeographic structure of the Lutzomyia longipalpis species complex (Diptera: Psychodidae) from Brazil and Venezuela.
The American journal of tropical medicine and hygiene, 2005Co-Authors: Phillip C. Watts, N.a. De Souza, Reginaldo Peçanha Brazil, James G. C. Hamilton, R. D. Ward, M. Dora Feliciangeli, Harry Noyes, Stephen J. Kemp, R. MaingonAbstract:Lutzomyia longipalpis, a sibling complex, is the main vector of Leishmania chagasi/infantum. Discriminating between siblings is important as they may differ in vectorial capacity. Lutzomyia longipalpis populations display distinct male sex pheromone chemotypes. We investigated the phylogeographic pattern of variation at microsatellite loci from 11 populations from Brazil and Venezuela related to their male pheromone. Temporal genetic differentiation was mostly not significant at the same site. Spatial genetic differentiation was, however, strong, although there was only a weak relationship between genetic differentiation and the geographic distance separating the samples (r2 < 0.10); geographic separation explained a much greater (54-97%) percentage of the genetic differences among populations when samples with the same pheromone type were analyzed separately. A cluster analysis showed five groups: Lu. cruzi (Brazil) and Lu. pseudolongipalpis (Venezuela) as separate species, two (mostly 9-methyl-germacrene-B) Venezuelan and Brazilian groups, and a very distinct cluster of Brazilian cembrene populations.
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Copulation songs in three siblings of Lutzomyia longipalpis (Diptera: Psychodidae)
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2004Co-Authors: N.a. De Souza, Charalambos P. Kyriacou, Richard D. Ward, James G. C. Hamilton, Alexandre A. PeixotoAbstract:We present the results of recording male courtship songs of the sandfly Lutzomyia longipalpis. The striking differences in the songs from 3 Brazilian populations of this sandfly with 3 distinct male pheromones support the 3 sibling species previously proposed based on this characteristic.
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Spectral sensitivity in the eyes of male and female Lutzomyia longipalpis sandflies.
Medical and veterinary entomology, 1996Co-Authors: Helen E. Mellor, James G. C. Hamilton, M. AndersonAbstract:Using electroretinogram recordings, the response of Lutzomyia longipalpis sandfly eyes to a range of wavelengths of light was measured, and spectral sensitivity determined. The eyes of both male and female adult sandflies were found to respond maximally to light in the ultraviolet region (at 340 nm) with a secondary peak in the blue-green-yellow region at 520 nm for females and 546 nm for males. The Mann-Whitney U test showed no significant differences between males and females at corresponding wavelengths.
R. D. Ward - One of the best experts on this subject based on the ideXlab platform.
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the Lutzomyia longipalpis species complex does population sub structure matter to leishmania transmission
Trends in Parasitology, 2008Co-Authors: R. Maingon, R. D. Ward, J G C Hamilton, Luiz Guilherme Soares Da Rocha Bauzer, Alexandre A. PeixotoAbstract:Leishmania chagasi causes visceral leishmaniasis and, to a lesser extent, atypical cutaneous leishmaniasis in Central and South America. Its main sand fly vector, Lutzomyia longipalpis s.l. (Diptera: Psychodidae) displays a complex population structure that might contribute to the observed clinical pleomorphism and to recent major urban epidemics. This article summarises our understanding on reproductive barriers and hybridisation among this vector's sibling incipient species. Identifying genes important for sand fly ecological adaptability and sand fly– Leishmania genetic co-variation could be helpful for interrupting Leishmania transmission.
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Male sex pheromones and the phylogeographic structure of the Lutzomyia longipalpis species complex (Diptera: Psychodidae) from Brazil and Venezuela.
The American journal of tropical medicine and hygiene, 2005Co-Authors: Phillip C. Watts, N.a. De Souza, Reginaldo Peçanha Brazil, James G. C. Hamilton, R. D. Ward, M. Dora Feliciangeli, Harry Noyes, Stephen J. Kemp, R. MaingonAbstract:Lutzomyia longipalpis, a sibling complex, is the main vector of Leishmania chagasi/infantum. Discriminating between siblings is important as they may differ in vectorial capacity. Lutzomyia longipalpis populations display distinct male sex pheromone chemotypes. We investigated the phylogeographic pattern of variation at microsatellite loci from 11 populations from Brazil and Venezuela related to their male pheromone. Temporal genetic differentiation was mostly not significant at the same site. Spatial genetic differentiation was, however, strong, although there was only a weak relationship between genetic differentiation and the geographic distance separating the samples (r2 < 0.10); geographic separation explained a much greater (54-97%) percentage of the genetic differences among populations when samples with the same pheromone type were analyzed separately. A cluster analysis showed five groups: Lu. cruzi (Brazil) and Lu. pseudolongipalpis (Venezuela) as separate species, two (mostly 9-methyl-germacrene-B) Venezuelan and Brazilian groups, and a very distinct cluster of Brazilian cembrene populations.
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Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis.
Medical and veterinary entomology, 1994Co-Authors: M. J. Dougherty, James G. C. Hamilton, R. D. WardAbstract:Abstract. Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 ug of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.
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Semiochemical mediation of oviposition by the phlebotomine sandfly Lutzomyia longipalpis
Medical and veterinary entomology, 1993Co-Authors: M. J. Dougherty, James G. C. Hamilton, R. D. WardAbstract:Extracts of rabbit food, hay and rabbit faeces elicited a positive oviposition response from gravid female Lutzomyia longipalpis sandflies (Diptera: Phlebotominae). Combined extract of rabbit food and oviposition pheromone had a synergistic effect on sandfly egg‐laying, greatly increasing the number of eggs laid and resulting in a highly targeted response. Individually tubed flies, exposed to the combined extract, were shown to be 3.5 times more likely to survive oviposition and laid 2.5 times more eggs than control flies. A laboratory oviposition trap baited with the combined extract was tested in a cage and caught 31 (62%) of 50 gravid L.longipalpis over a 72h period.
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Gas-chromatographic analysis of Lutzomyia longipalpis tergal pheromone gland extract.
Parassitologia, 1991Co-Authors: James G. C. Hamilton, R. D. WardAbstract:Lutzomyia longipalpis is the vector of visceral leishmaniasis (VL) in South America. Previous gas-chromatographic (GC) analyses of tergal gland extracts of Lu. longipalpis have demonstrated two distinct pheromone types. These have been classified as a farnesene/homofarnesene type compound and a diterpene type compound. GC analysis of tergal gland extracts of Lu. longipalpis from other areas of South America demonstrates that there may be other different pheromone producing forms of this sandfly. Gland extracts are more complex than has previously been indicated, and they have been shown to differ in the chemicals present and in the ratio of these compounds to each other.
Nagila Francinete Costa Secundino - One of the best experts on this subject based on the ideXlab platform.
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exploring Lutzomyia longipalpis sand flies vector competence to leishmania major parasites
The Journal of Infectious Diseases, 2020Co-Authors: Pedro Cecilio, Ana Clara Araujo Machado Pires, Jesus G Valenzuela, Paulo Filemon Paolucci Pimenta, Anabela Cordeirodasilva, Nagila Francinete Costa SecundinoAbstract:: Lutzomyia longipalpis sand flies are the major natural vector of Leishmania infantum parasites, responsible for the transmission of visceral leishmaniasis in the New World. Several experimental studies highlight its permissive nature, translated to the ability to sustain the development of different Leishmania species. Still, no study deeply explored the potential vector competence of Lu. longipalpis sand flies to Leishmania species other than L. infantum. Here, we show that Lu. longipalpis is a competent vector of L. major parasites, being able to acquire parasites from cutaneous leishmaniasis active lesions, sustain mature infections, and transmit them to naive hosts, causing disease.
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Exploring Lutzomyia longipalpis Sand Fly Vector Competence for Leishmania major Parasites.
The Journal of infectious diseases, 2020Co-Authors: Pedro Cecilio, Ana Clara Araujo Machado Pires, Jesus G Valenzuela, Paulo Filemon Paolucci Pimenta, Nagila Francinete Costa Secundino, Anabela Cordeiro-da-silva, Fabiano OliveiraAbstract:Lutzomyia longipalpis sand flies are the major natural vector of Leishmania infantum parasites, responsible for transmission of visceral leishmaniasis in the New World. Several experimental studies have demonstrated the ability of Lu. longipalpis to sustain development of different Leishmania species. However, no study had explored in depth the potential vector competence of Lu. longipalpis for Leishmania species other than L. infantum. Here, we show that Lu. longipalpis is a competent vector of L. major parasites, being able to acquire parasites from active cutaneous leishmaniasis lesions, sustain mature infections, and transmit them to naive hosts, causing disease.
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Lutzomyia longipalpis peritrophic matrix: formation, structure, and chemical composition.
Journal of medical entomology, 2005Co-Authors: Nagila Francinete Costa Secundino, I. Eger-mangrich, Érika Martins Braga, M. M. Santoro, Paulo F. P. PimentaAbstract:Abstract Sandflies are vectors of several pathogens, constituting serious health problems. Lutzomyia longipalpis (Lutz & Neiva, 1912) is the main vector of Leishmania chagasi, agent of visceral leishmaniasis. They synthesize a thick bag-like structure that surrounds the bloodmeal, named peritrophic matrix (PM). One of the major roles of PM in blood-fed insects includes protection against ingested pathogens by providing a defensive barrier to their development. We used traditional and modern morphological methods as well as biochemical and immunolabeling tools to define details of the PM structure of the Lu. longipalpis sandfly, including composition, synthesis, and degradation. The kinetics of PM formation and degradation was found to be related to the ingestion and time of digestion of the bloodmeal. The midgut changes its size and morphology after the blood ingestion and during the course of digestion. A striking morphological modification takes place in the midgut epithelium after the stretching caused...
