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Stanislava Fialová - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial and antioxidant properties of four Lycopus taxa and an interaction study of their major compounds
Molecules, 2020Co-Authors: Eva Trajčíková, Marek Straka, Aneta Lichvarikova, Svetlana Dokupilova, Iveta Cicova, Lívia Slobodníková, M Nagy, Elena Kurin, Pavel Mučaji, Stanislava FialováAbstract:The compositions of leaf infusions of three genotypes of Lycopus europaeus L. with origins in central Europe, namely L. europaeus A (LeuA), L. europaeus B (LeuB), and L. europaeus C (LeuC), and one genotype of L. exaltatus (Lex), were examined by LC-MS-DAD (Liquid Chromatography Mass Spectrometry and Diode Array Detection) analysis. This revealed the presence of thirteen compounds belonging to the groups of phenolic acids and flavonoids, with a predominance of rosmarinic acid (RA) and luteolin-7-O-glucuronide (LGlr). The antimicrobial activity of leaf infusions was tested on the collection strains of Gram-positive and Gram-negative bacteria, and on the clinical Staphylococcus aureus strains. We detected higher activity against Gram-positive bacteria, of which the most susceptible strains were those of Staphylococcus aureus, including methicillin-resistant and poly-resistant strains. Furthermore, we examined the antioxidant activity of leaf infusions using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods, and on NIH/3T3 cell lines using dichlorodihydrofluorescein diacetate (DCFH-DA). We also studied the mutual interactions between selected infusions, namely RA and/or LGlr. In the mixtures of leaf infusion and RA or LGlr, we observed slight synergism and a high dose reduction index in most cases. This leads to the beneficial dose reduction at a given antioxidant effect level in mixtures compared to the doses of the parts used alone. Therefore, our study draws attention to further applications of the Lycopus leaves as a valuable alternative source of natural antioxidants and as a promising topical antibacterial agent for medicinal use.
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Lycopus europaeus phenolic fingerprint antioxidant activity and antimicrobial effect on clinical staphylococcus aureus strains
Natural Product Research, 2015Co-Authors: Stanislava Fialová, Lucia Veizerova, Lívia Slobodníková, Daniel GrancaiAbstract:Lycopus europaeus L. leaves water extract (LEL) was subjected to phytochemical analysis, and evaluated for its antibacterial and antioxidant effects. Antibacterial activity testing was performed on Staphylococcus aureus clinical strains from catheter-related and skin infections by broth microdilution test. LEL showed bactericidal activity at concentrations from 2500 to 5000 μg/mL against all, including methicillin resistant and polyresistant nosocomial, strains. Antioxidant activity was examined using DPPH and ABTS (11.3 and 9.8 μg/mL, respectively) and by ferric reducing ability of the plasma method (891 μmol AAE/g dry extract). Phytochemical analysis of LEL was performed by LC-DAD-MS/MS. Ten phenolic compounds were identified; two minor compounds (glucopyranosyl rosmarinic acid and sagerinig acid) have not been described in Lycopus yet. The major compounds, considered to be responsible for biological activities detected in the study, were determined as rosmarinic acid (76 mg/g) and luteolin-7-O-glucuron...
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Lycopus europaeus: phenolic fingerprint, antioxidant activity and antimicrobial effect on clinical Staphylococcus aureus strains
2015Co-Authors: Stanislava Fialová, Lucia Veizerova, Lívia Slobodníková, Daniel GranČaiAbstract:Lycopus europaeus L. leaves water extract (LEL) was subjected to phytochemical analysis, and evaluated for its antibacterial and antioxidant effects. Antibacterial activity testing was performed on Staphylococcus aureus clinical strains from catheter-related and skin infections by broth microdilution test. LEL showed bactericidal activity at concentrations from 2500 to 5000 μg/mL against all, including methicillin resistant and polyresistant nosocomial, strains. Antioxidant activity was examined using DPPH and ABTS (11.3 and 9.8 μg/mL, respectively) and by ferric reducing ability of the plasma method (891 μmol AAE/g dry extract). Phytochemical analysis of LEL was performed by LC-DAD-MS/MS. Ten phenolic compounds were identified; two minor compounds (glucopyranosyl rosmarinic acid and sagerinig acid) have not been described in Lycopus yet. The major compounds, considered to be responsible for biological activities detected in the study, were determined as rosmarinic acid (76 mg/g) and luteolin-7-O-glucuronide (23 mg/g). L. europaeus arises from our study as a promising source of antibacterial agent for topical usage.
Abdul Aziz - One of the best experts on this subject based on the ideXlab platform.
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in vitro anti inflammatory activity of Lycopus europaeus linn
2014Co-Authors: Abdul Aziz, Shahid Masood Raza, Musaddique Hussain, Zahid Manzoor, Shaukat Hussain Munawar, Imran Khan, Muhammad SaleemAbstract:europaeus is an ayurvedic medicine which is used as anti-inflammatory agent. Therefore, our investigation was aimed to screen antiinflammatory activity of Lycopus europaeus by membrane stabilizing and protein inhibitory methods. The prevention of hypotonicity induced human red blood cells (HRBC) membrane lysis and protein inhibition was taken as a measure of the anti-inflammatory activity. The potency of the Lycopus europaeus was compared with standard diclofenac sodium. Lycopus europaeus showed significant membrane stabilizing activity of 73.81% and protein inhibition activity of 93.01% at concentration of 200 μg/mL.
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role of Lycopus europaeus methanolic extract in the enhancement of wound healing activity in experimental rats
2014Co-Authors: Abdul Aziz, Shaukat Hussain Munawar, Zahid Manzoor, Imran Khan, Muhammad Mudassar AshrafAbstract:wound healing activity of topically applied methanol extract of Lycopus europaeus was evaluated in wistar rat by excision wound model for a period of 12 days. The extract was prepared as ointment form (5 and 10% w/w) and applied on Wistar rats. Neomycin ointment 0.5%w/w was used as standard drug. The healing of the wound was assessed by the rate of wound contraction, period of epithelialisation, skin breaking strength. Both the ointments (5% and 10% w/w) of Lycopus europaeus extract promoted the wound-healing activity significantly when compared to the standard drug. High rate of wound contraction, decrease the period for epithelialisation, high skin breaking strength were observed in animals treated with 10% w/w extract ointment when compared to the control group of animals. So methanol extract of Lycopus europaeus in the form of 10% ointment promote wound-healing activity better than the former concentration, 5%. The result obtained from this study indicates that methanol extract of Lycopus europaeus accelerates the wound healing process by decreasing the surface area of the wound. The efficacy of this plant in wound healing may be due to its epithelization, thereby justifying the traditional claim.
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antinociceptive and anti inflammatory activity of the extract of Lycopus europaeus on laboratory animals
International Journal of Research and Development in Pharmacy and Life Sciences, 2014Co-Authors: Abdul Aziz, Aqsa Afzal, Musaddique Hussain, Imran Khan, Muhammad Bilal AhmedAbstract:Aims: To evaluate the analgesic and anti-inflammatory effects of methanolic extract of Lycopus europaeus on experimental animal models. Methodology: The analgesic activity was evaluated by hot plate, acetic acid induced writhing and formalin induced writhing method in Swiss Albino mice divided into 4 different groups (control, standard diclofenac sodium and extract at two different doses of 250 and 500 mg/kg BW). The extract was also investigated for the anti-inflammatory effect on Long Evans rats using carrageenan induced rat paw edema method. For anti-inflammatory study, 24 rats were divided into 4 different groups each receiving either distilled water, standard drug or the extract at the doses of 250 and 500 mg/kg BW. Results: Phytochemical analysis of the extract revealed the presence of tannins, flavonoids and terpenoids. The extract elicited a highly significant (p<0.001) analgesic activity in a dose dependent manner on hot plate method, acetic acid induced writhing test and also on both the early and late phases of formalin test at the doses employed. In the hot plate method, the extract increased the reaction time of heat sensation to 60.81% and 66.52% at the doses of 250 and 500 mg/kg BW respectively while that of the standard drug was 57.40% at the 3rd hour of study. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 62.87% and 70.66% at 250 and 500 mg/kg doses respectively (p<0.001) which were even better than the standard drug diclofenac sodium (50.30%). The extract also significantly inhibited the licking response at the dose of 500 mg/kg in both the early phase (55.11%, p<0.01) and the late phase (66.43%, p<0.01) of formalin test while the standard drug inhibited by 52.27% and 72.03%, respectively. The oral administration of the extract significantly (p<0.001) inhibited inflammatory response induced by carrageenan in a dose dependent fashion. The most prominent inhibition of 61.68% (250 mg/kg) and 73.65% (500 mg/kg) were observed at the 4th hour of study. Conclusion: The central and peripheral analgesic as well as anti-inflammatory effect of the methanolic extract of Lycopus europaeus may be due to the presence of various chemical constituents specially flavonoids, tannins, alkaloids or terpenoids. These experimental findings would further establish the scientific basis of the traditional uses of the plant in the management and/or control of pain as well as inflammatory conditions.Keywords: Basic needs, biodiversity conservation, women’s basic understanding.
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Pharmacological evaluation of Sedative and Hypnotic activities of methanolic extract of Lycopus europaeus in mice
2013Co-Authors: Abdul Aziz, Imran KhanAbstract:The methanolic extract of Lycopus europaeus was evaluated on central nervous system. The methanolic extract produced significant sedative effect at the doses of 200, 400 and 600 mg/kg (by oral route), compared to reference substance diazepam in hole board and thiopental -induced sleeping time methods. While the hypnotic effect was calculated at the doses of 800 and 1000 mg/kg via oral pathway significantly reduced in both the reestablishment time and number of head dips during the traction and hole-board tests. In conclusion, these results suggest that the methanolic extract of Lycopus europaeus possess potent sedative and hypnotic activities, which supported its therapeutic use for insomnia.
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evaluation of antitussive activity of Lycopus europaeus on cough reflex induced by different cough induced models in mice
2013Co-Authors: Abdul Aziz, Aisha Perveen, Shahnaz Agha, Shaukat Hussain, Imran Khan, Zahid ManzoorAbstract:The present study was carried out to elucidate the potential of, methanol extract of Lycopus europaeus plant on antitussive activity in albino mice. The methanol extract (yield 12.5% w/w with respected to dry powdered plant material) was selected for all experimental procedure. Antitussive activity of the methanolic extract of Lycopus europaeus (Le.cr) was investigated for its effect on a cough model induced by sulphur dioxide gas and Ammonium liquor induced cough in mice. Results were revealed that the Le.cr was found to produce significant antitussive activity (P<0.001) when compared with control, codeine phosphate and Dextramethorphane in a dose dependent manner. High dose of Le.cr of (500 mg/kg) showed maximum inhibition of cough by 61.21% and 56.63% induced by both inducers. It concludes that Le.cr possessed remarkable antitussive effect, which provides pharmacological evidence in support of folklore-claim of Lycopus europaeus as an antitussive agent.
Zorica Stojanovicradic - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial phenolic abietane diterpene from Lycopus europaeus l lamiaceae
ChemInform, 2010Co-Authors: Niko Radulovic, Marija S Denic, Zorica StojanovicradicAbstract:A new acetylated highly oxygenated abietane-type diterpenoid named euroabienol (I) is isolated from Lycopus europaeus L.
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antimicrobial phenolic abietane diterpene from Lycopus europaeus l lamiaceae
Bioorganic & Medicinal Chemistry Letters, 2010Co-Authors: Niko Radulovic, Marija S Denic, Zorica StojanovicradicAbstract:A new acetylated highly oxygenated abietane-type diterpenoid named euroabienol was isolated in pure state from Lycopus europaeus L. (Lamiaceae) fruits and its structure elucidated through both extensive spectral and chemical means. The presence of a phenolic C ring with a rare substitution pattern in euroabienol and its high relative amount in the fruits (1%, based on the weight of the fruits) urged us to try to establish its possible biological role. Thus, it was screened for its in vitro antimicrobial activity against fifteen strains of bacteria and six fungal strains. Euroabienol showed a broad spectrum of activity and probably is a first line defense plant metabolite against pathogen attack. This is the first report on the occurrence of abietanes in the genus Lycopus.
Imran Khan - One of the best experts on this subject based on the ideXlab platform.
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in vitro anti inflammatory activity of Lycopus europaeus linn
2014Co-Authors: Abdul Aziz, Shahid Masood Raza, Musaddique Hussain, Zahid Manzoor, Shaukat Hussain Munawar, Imran Khan, Muhammad SaleemAbstract:europaeus is an ayurvedic medicine which is used as anti-inflammatory agent. Therefore, our investigation was aimed to screen antiinflammatory activity of Lycopus europaeus by membrane stabilizing and protein inhibitory methods. The prevention of hypotonicity induced human red blood cells (HRBC) membrane lysis and protein inhibition was taken as a measure of the anti-inflammatory activity. The potency of the Lycopus europaeus was compared with standard diclofenac sodium. Lycopus europaeus showed significant membrane stabilizing activity of 73.81% and protein inhibition activity of 93.01% at concentration of 200 μg/mL.
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role of Lycopus europaeus methanolic extract in the enhancement of wound healing activity in experimental rats
2014Co-Authors: Abdul Aziz, Shaukat Hussain Munawar, Zahid Manzoor, Imran Khan, Muhammad Mudassar AshrafAbstract:wound healing activity of topically applied methanol extract of Lycopus europaeus was evaluated in wistar rat by excision wound model for a period of 12 days. The extract was prepared as ointment form (5 and 10% w/w) and applied on Wistar rats. Neomycin ointment 0.5%w/w was used as standard drug. The healing of the wound was assessed by the rate of wound contraction, period of epithelialisation, skin breaking strength. Both the ointments (5% and 10% w/w) of Lycopus europaeus extract promoted the wound-healing activity significantly when compared to the standard drug. High rate of wound contraction, decrease the period for epithelialisation, high skin breaking strength were observed in animals treated with 10% w/w extract ointment when compared to the control group of animals. So methanol extract of Lycopus europaeus in the form of 10% ointment promote wound-healing activity better than the former concentration, 5%. The result obtained from this study indicates that methanol extract of Lycopus europaeus accelerates the wound healing process by decreasing the surface area of the wound. The efficacy of this plant in wound healing may be due to its epithelization, thereby justifying the traditional claim.
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antinociceptive and anti inflammatory activity of the extract of Lycopus europaeus on laboratory animals
International Journal of Research and Development in Pharmacy and Life Sciences, 2014Co-Authors: Abdul Aziz, Aqsa Afzal, Musaddique Hussain, Imran Khan, Muhammad Bilal AhmedAbstract:Aims: To evaluate the analgesic and anti-inflammatory effects of methanolic extract of Lycopus europaeus on experimental animal models. Methodology: The analgesic activity was evaluated by hot plate, acetic acid induced writhing and formalin induced writhing method in Swiss Albino mice divided into 4 different groups (control, standard diclofenac sodium and extract at two different doses of 250 and 500 mg/kg BW). The extract was also investigated for the anti-inflammatory effect on Long Evans rats using carrageenan induced rat paw edema method. For anti-inflammatory study, 24 rats were divided into 4 different groups each receiving either distilled water, standard drug or the extract at the doses of 250 and 500 mg/kg BW. Results: Phytochemical analysis of the extract revealed the presence of tannins, flavonoids and terpenoids. The extract elicited a highly significant (p<0.001) analgesic activity in a dose dependent manner on hot plate method, acetic acid induced writhing test and also on both the early and late phases of formalin test at the doses employed. In the hot plate method, the extract increased the reaction time of heat sensation to 60.81% and 66.52% at the doses of 250 and 500 mg/kg BW respectively while that of the standard drug was 57.40% at the 3rd hour of study. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 62.87% and 70.66% at 250 and 500 mg/kg doses respectively (p<0.001) which were even better than the standard drug diclofenac sodium (50.30%). The extract also significantly inhibited the licking response at the dose of 500 mg/kg in both the early phase (55.11%, p<0.01) and the late phase (66.43%, p<0.01) of formalin test while the standard drug inhibited by 52.27% and 72.03%, respectively. The oral administration of the extract significantly (p<0.001) inhibited inflammatory response induced by carrageenan in a dose dependent fashion. The most prominent inhibition of 61.68% (250 mg/kg) and 73.65% (500 mg/kg) were observed at the 4th hour of study. Conclusion: The central and peripheral analgesic as well as anti-inflammatory effect of the methanolic extract of Lycopus europaeus may be due to the presence of various chemical constituents specially flavonoids, tannins, alkaloids or terpenoids. These experimental findings would further establish the scientific basis of the traditional uses of the plant in the management and/or control of pain as well as inflammatory conditions.Keywords: Basic needs, biodiversity conservation, women’s basic understanding.
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Pharmacological evaluation of Sedative and Hypnotic activities of methanolic extract of Lycopus europaeus in mice
2013Co-Authors: Abdul Aziz, Imran KhanAbstract:The methanolic extract of Lycopus europaeus was evaluated on central nervous system. The methanolic extract produced significant sedative effect at the doses of 200, 400 and 600 mg/kg (by oral route), compared to reference substance diazepam in hole board and thiopental -induced sleeping time methods. While the hypnotic effect was calculated at the doses of 800 and 1000 mg/kg via oral pathway significantly reduced in both the reestablishment time and number of head dips during the traction and hole-board tests. In conclusion, these results suggest that the methanolic extract of Lycopus europaeus possess potent sedative and hypnotic activities, which supported its therapeutic use for insomnia.
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evaluation of antitussive activity of Lycopus europaeus on cough reflex induced by different cough induced models in mice
2013Co-Authors: Abdul Aziz, Aisha Perveen, Shahnaz Agha, Shaukat Hussain, Imran Khan, Zahid ManzoorAbstract:The present study was carried out to elucidate the potential of, methanol extract of Lycopus europaeus plant on antitussive activity in albino mice. The methanol extract (yield 12.5% w/w with respected to dry powdered plant material) was selected for all experimental procedure. Antitussive activity of the methanolic extract of Lycopus europaeus (Le.cr) was investigated for its effect on a cough model induced by sulphur dioxide gas and Ammonium liquor induced cough in mice. Results were revealed that the Le.cr was found to produce significant antitussive activity (P<0.001) when compared with control, codeine phosphate and Dextramethorphane in a dose dependent manner. High dose of Le.cr of (500 mg/kg) showed maximum inhibition of cough by 61.21% and 56.63% induced by both inducers. It concludes that Le.cr possessed remarkable antitussive effect, which provides pharmacological evidence in support of folklore-claim of Lycopus europaeus as an antitussive agent.
Bernt Krebs - One of the best experts on this subject based on the ideXlab platform.
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purification and spectroscopic studies on catechol oxidases from Lycopus europaeus and populus nigra evidence for a dinuclear copper center of type 3 and spectroscopic similarities to tyrosinase and hemocyanin
Journal of Biological Inorganic Chemistry, 1999Co-Authors: Annette Rompel, Dirk Meiwes, Klaudia Buldtkarentzopoulos, Renee Dillinger, Felix Tuczek, Helmut Fischer, Herbert Witzel, Bernt KrebsAbstract:We purified two catechol oxidases from Lycopus europaeus and Populus nigra which only catalyze the oxidation of catechols to quinones without hydroxylating tyrosine. The molecular mass of the Lycopus enzyme was determined to 39 800 Da and the mass of the Populus enzyme was determined to 56 050 Da. Both catechol oxidases are inhibited by thiourea, N-phenylthiourea, dithiocarbamate, and cyanide, but show different pH behavior using catechol as substrate. Atomic absorption spectroscopic analysis found 1.5 copper atoms per protein molecule. Using EPR spectroscopy we determined 1.8 Cu per molecule catechol oxidase. Furthermore, EPR spectroscopy demonstrated that catechol oxidase is a copper enzyme of type 3. The lack of an EPR signal is due to strong antiferromagnetic coupling that requires a bridging ligand between the two copper ions in the met preparation. Addition of H2O2 to both enzymes leads to oxy catechol oxidase. In the UV/Vis spectrum two new absorption bands occur at 345 nm and 580 nm. In accordance with the oxy forms of hemocyanin and tyrosinase the absorption band at 345 nm is due to an O22– (πσ*)→Cu(II) (d x2–y2 ) charge transfer (CT) transition. The absorption band at 580 nm corresponds to the second O22– (πv*)→Cu(II) (d x2–y2 ) CT transition. The UV/Vis bands in combination with the resonance Raman spectra of oxy catechol oxidase indicate a μ-η2 : η2 binding mode for dioxygen. The intense resonance Raman peak at 277 cm–1, belonging to a Cu-N (axial His) stretching mode, suggests that catechol oxidase has six terminal His ligands, as known for molluscan and arthropodan hemocyanin.
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substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation1
FEBS Letters, 1999Co-Authors: Annette Rompel, Dirk Meiwes, Klaudia Buldtkarentzopoulos, Annette Magrini, Carsten Gerdemann, Christoph Eicken, Helmut Fischer, Bernt KrebsAbstract:The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. The enzyme catalyzes the oxidation of o-diphenols to o-quinones without hydroxylating monophenols, the additional activity of tyrosinase. Substrates containing a -COOH group are inhibitors for catechol oxidase. The products of enzymic oxidation of caffeic acid were analyzed and isolated by HPLC with diode array detection. The neolignans of the 2,3-dihydro-1,4-benzodioxin type (3, 6–8), 6,7-dihydroxy-1-(3,4-dihydroxyphenyl)-2,3-dicarboxy-1,2-dihydronaphthaline (1) 6,7-dihydroxy-1-(3,4-dihydroxyphenyl)-3-carboxynaphthaline (5) and 2,6-bis-(3′,4′-dihydroxyphenyl)-1-carboxy-3-oxacyclo-(3,0)-pentan-2-on-1-ene (4) were formed. A reaction mechanism for the formation of (1, 4 and 5) is discussed.
