The Experts below are selected from a list of 101034 Experts worldwide ranked by ideXlab platform
Lia J Zallar - One of the best experts on this subject based on the ideXlab platform.
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development and initial characterization of a novel ghrelin receptor crispr cas9 knockout Wistar Rat model
International Journal of Obesity, 2019Co-Authors: Lia J Zallar, Brendan J Tunstall, Christopher T Richie, Yajun Zhang, Zhibing You, Eliot L Gardner, Markus Heilig, James PickelAbstract:Ghrelin, a stomach-derived hormone implicated in numerous behaviors including feeding, reward, stress, and addictive behaviors, acts by binding to the growth hormone secretagogue receptor (GHSR). Here, we present the development, verification, and initial characterization of a novel GHSR knockout (KO) Wistar Rat model created with CRISPR genome editing. Using CRISPR/Cas9, we developed a GHSR KO in a Wistar background. Loss of GHSR mRNA expression was histologically verified using RNAscope in wild-type (WT; n = 2) and KO (n = 2) Rats. We tested the effects of intraperitoneal acyl-ghrelin administRation on food consumption and plasma growth hormone (GH) concentRations in WT (n = 8) and KO (n = 8) Rats. We also analyzed locomotion, food consumption, and body fat composition in these animals. Body weight was monitored from early development to adulthood. The RNAscope analysis revealed an abundance of GHSR mRNA expression in the hypothalamus, midbrain, and hippocampus in WTs, and no observed probe binding in KOs. Ghrelin administRation increased plasma GH levels (p = 0.0067) and food consumption (p = 0.0448) in WT Rats but not KOs. KO Rats consumed less food overall at basal conditions and weighed significantly less compared with WTs throughout development (p = 0.0001). Compared with WTs, KOs presented higher concentRations of brown adipose tissue (BAT; p = 0.0322). We have verified GHSR deletion in our KO model using histological, physiological, neuroendocrinological, and behavioral measures. Our findings indicate that GHSR deletion in Rats is not only associated with a lack of response to ghrelin, but also associated with decreases in daily food consumption and body growth, and increases in BAT. This GHSR KO Wistar Rat model provides a novel tool for studying the role of the ghrelin system in obesity and in a wide range of medical and neuropsychiatric disorders.
James Pickel - One of the best experts on this subject based on the ideXlab platform.
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development and initial characterization of a novel ghrelin receptor crispr cas9 knockout Wistar Rat model
International Journal of Obesity, 2019Co-Authors: Lia J Zallar, Brendan J Tunstall, Christopher T Richie, Yajun Zhang, Zhibing You, Eliot L Gardner, Markus Heilig, James PickelAbstract:Ghrelin, a stomach-derived hormone implicated in numerous behaviors including feeding, reward, stress, and addictive behaviors, acts by binding to the growth hormone secretagogue receptor (GHSR). Here, we present the development, verification, and initial characterization of a novel GHSR knockout (KO) Wistar Rat model created with CRISPR genome editing. Using CRISPR/Cas9, we developed a GHSR KO in a Wistar background. Loss of GHSR mRNA expression was histologically verified using RNAscope in wild-type (WT; n = 2) and KO (n = 2) Rats. We tested the effects of intraperitoneal acyl-ghrelin administRation on food consumption and plasma growth hormone (GH) concentRations in WT (n = 8) and KO (n = 8) Rats. We also analyzed locomotion, food consumption, and body fat composition in these animals. Body weight was monitored from early development to adulthood. The RNAscope analysis revealed an abundance of GHSR mRNA expression in the hypothalamus, midbrain, and hippocampus in WTs, and no observed probe binding in KOs. Ghrelin administRation increased plasma GH levels (p = 0.0067) and food consumption (p = 0.0448) in WT Rats but not KOs. KO Rats consumed less food overall at basal conditions and weighed significantly less compared with WTs throughout development (p = 0.0001). Compared with WTs, KOs presented higher concentRations of brown adipose tissue (BAT; p = 0.0322). We have verified GHSR deletion in our KO model using histological, physiological, neuroendocrinological, and behavioral measures. Our findings indicate that GHSR deletion in Rats is not only associated with a lack of response to ghrelin, but also associated with decreases in daily food consumption and body growth, and increases in BAT. This GHSR KO Wistar Rat model provides a novel tool for studying the role of the ghrelin system in obesity and in a wide range of medical and neuropsychiatric disorders.
Yajun Zhang - One of the best experts on this subject based on the ideXlab platform.
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development and initial characterization of a novel ghrelin receptor crispr cas9 knockout Wistar Rat model
International Journal of Obesity, 2019Co-Authors: Lia J Zallar, Brendan J Tunstall, Christopher T Richie, Yajun Zhang, Zhibing You, Eliot L Gardner, Markus Heilig, James PickelAbstract:Ghrelin, a stomach-derived hormone implicated in numerous behaviors including feeding, reward, stress, and addictive behaviors, acts by binding to the growth hormone secretagogue receptor (GHSR). Here, we present the development, verification, and initial characterization of a novel GHSR knockout (KO) Wistar Rat model created with CRISPR genome editing. Using CRISPR/Cas9, we developed a GHSR KO in a Wistar background. Loss of GHSR mRNA expression was histologically verified using RNAscope in wild-type (WT; n = 2) and KO (n = 2) Rats. We tested the effects of intraperitoneal acyl-ghrelin administRation on food consumption and plasma growth hormone (GH) concentRations in WT (n = 8) and KO (n = 8) Rats. We also analyzed locomotion, food consumption, and body fat composition in these animals. Body weight was monitored from early development to adulthood. The RNAscope analysis revealed an abundance of GHSR mRNA expression in the hypothalamus, midbrain, and hippocampus in WTs, and no observed probe binding in KOs. Ghrelin administRation increased plasma GH levels (p = 0.0067) and food consumption (p = 0.0448) in WT Rats but not KOs. KO Rats consumed less food overall at basal conditions and weighed significantly less compared with WTs throughout development (p = 0.0001). Compared with WTs, KOs presented higher concentRations of brown adipose tissue (BAT; p = 0.0322). We have verified GHSR deletion in our KO model using histological, physiological, neuroendocrinological, and behavioral measures. Our findings indicate that GHSR deletion in Rats is not only associated with a lack of response to ghrelin, but also associated with decreases in daily food consumption and body growth, and increases in BAT. This GHSR KO Wistar Rat model provides a novel tool for studying the role of the ghrelin system in obesity and in a wide range of medical and neuropsychiatric disorders.
P.s. Lonkar - One of the best experts on this subject based on the ideXlab platform.
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Testicular hydrops in Wistar Rat - A case rerort
Indian Journal of Veterinary Pathology, 2012Co-Authors: A. Adak, M.c. Prasad, M.g. Brahmankar, U.m. Kapurkar, P.s. Lonkar, M.v. PatelAbstract:A case of bilateral testicular hydrops has been reported in a 26 months old male Wistar Rat out of over 20 thousand Wistar Rats necropsied over the period. Grossly, both the testes were enlarged and testicular parenchyma was floating amidst the pool of fluid present inside the tunica albuginea/capsule. Histomorphology exhibited marked interstitial oedema with marked reduction in the number of cut sections of seminiferous tubules showing degeneRative/atrophic changes. The exact etiology could not be ascertained.
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Histiocytic sarcoma in spleen of a Wistar Rat.
Indian Journal of Veterinary Pathology, 2010Co-Authors: M.c. Prasad, M.g. Brahmankar, Rajesh A. Vadaviya, U.m. Kapurkar, P.s. LonkarAbstract:A case of histiocytic sarcoma in the spleen of a 3 months old Wistar Rat has been recorded and discussed along with histopathological description.
M.c. Prasad - One of the best experts on this subject based on the ideXlab platform.
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Testicular hydrops in Wistar Rat - A case rerort
Indian Journal of Veterinary Pathology, 2012Co-Authors: A. Adak, M.c. Prasad, M.g. Brahmankar, U.m. Kapurkar, P.s. Lonkar, M.v. PatelAbstract:A case of bilateral testicular hydrops has been reported in a 26 months old male Wistar Rat out of over 20 thousand Wistar Rats necropsied over the period. Grossly, both the testes were enlarged and testicular parenchyma was floating amidst the pool of fluid present inside the tunica albuginea/capsule. Histomorphology exhibited marked interstitial oedema with marked reduction in the number of cut sections of seminiferous tubules showing degeneRative/atrophic changes. The exact etiology could not be ascertained.
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Histiocytic sarcoma in spleen of a Wistar Rat.
Indian Journal of Veterinary Pathology, 2010Co-Authors: M.c. Prasad, M.g. Brahmankar, Rajesh A. Vadaviya, U.m. Kapurkar, P.s. LonkarAbstract:A case of histiocytic sarcoma in the spleen of a 3 months old Wistar Rat has been recorded and discussed along with histopathological description.
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Hepatodiaphragmatic nodule (HDN): a congenital anomaly in Wistar Rat
Indian Journal of Veterinary Pathology, 2007Co-Authors: S.d. Godase, A. Adak, V.k. Goyal, R. A. Ugalmugle, P.b. Deshmukh, M.c. PrasadAbstract:A case of hepatodiaphragmatic nodule (HDN) in a Wistar Rat aged between 18 to 20 weeks has been reported. At necropsy, liver showed a round mass approximately 0.5 cm in diameter, protruding from the parietal surface of the right median lobe. Microscopically, the nodular portion was continuous with the hepatic parenchyma with a rounded outer contour. The occurrence of HDN, a congenital anomaly in Wistar Rats is rare i.e. 0.31% (2 out of 636 animals).
