The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Dolores Castro - One of the best experts on this subject based on the ideXlab platform.
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Immune gene expression in gilthead seabream (Sparus aurata) after Lymphocystis disease virus (LCDV-Sa) challenge resulting in asymptomatic infection.
Journal of applied microbiology, 2019Co-Authors: Rocío Leiva-rebollo, Juan J. Borrego, Alejandro M. Labella, Dolores CastroAbstract:Aims To determine the immune gene expression response of gilthead seabream (Sparus aurata) that is experimentally infected with the Lymphocystivirus LCDV-Sa. Methods and results Viral DNA and transcripts were detected by qPCR in all samples from fish injected with LCDV-Sa, demonstrating that the virus establish a systemic and asymptomatic infection. The expression of 23 immune-related genes was also analysed by RT-qPCR in the head kidney (HK) and intestine at several times post-infection (dpi). In HK, the expression of five type I interferon (IFN)-related genes (ifn, irf3, mx2, mx3 and isg15), il10 and ck10 was upregulated at 1-3 dpi, while genes related to the inflammation process (tnfα, il1s, il6, casp1) were not differentially expressed or even downregulated. The expression profile in the intestine was different regarding type I INF-related genes. An upregulated c3 and ighm expression was observed in both HK and intestine at 3-8 dpi. Finally, the transcription of nccrp1 and mhcIIα was induced in HK, whereas tcrβ expression was downregulated in both organs. Conclusions LCDV-Sa seems to trigger an immune response in gilthead seabream characterized by a partial activation of type I IFN system and a lack of systemic inflammatory response which may be related to viral persistence. Significance and impact of the study The immune response observed in gilthead seabream infected by LCDV-Sa could be implicated in the establishment of an asymptomatic persistent infection.
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Artemia spp., a Susceptible Host and Vector for Lymphocystis Disease Virus
Viruses, 2019Co-Authors: Estefania J. Valverde, Juan J. Borrego, Alejandro M. Labella, Dolores CastroAbstract:Different developmental stages of Artemia spp. (metanauplii, juveniles and adults) were bath-challenged with two isolates of the Lymphocystis disease virus (LCDV), namely, LCDV SA25 (belonging to the species Lymphocystis disease virus 3) and ATCC VR-342 (an unclassified member of the genus Lymphocystivirus). Viral quantification and gene expression were analyzed by qPCR at different times post-inoculation (pi). In addition, infectious titres were determined at 8 dpi by integrated cell culture (ICC)-RT-PCR, an assay that detects viral mRNA in inoculated cell cultures. In LCDV-challenged Artemia, the viral load increased by 2–3 orders of magnitude (depending on developmental stage and viral isolate) during the first 8–12 dpi, with viral titres up to 2.3 × 102 Most Probable Number of Infectious Units (MPNIU)/mg. Viral transcripts were detected in the infected Artemia, relative expression values showed a similar temporal evolution in the different experimental groups. Moreover, gilthead seabream (Sparus aurata) fingerlings were challenged by feeding on LCDV-infected metanauplii. Although no Lymphocystis symptoms were observed in the fish, the number of viral DNA copies was significantly higher at the end of the experimental trial and major capsid protein (mcp) gene expression was consistently detected. The results obtained support that LCDV infects Artemia spp., establishing an asymptomatic productive infection at least under the experimental conditions tested, and that the infected metanauplii are a vector for LCDV transmission to gilthead seabream.
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A new genotype of Lymphocystivirus isolated from cultured gilthead seabream, Sparus aurata L., and Senegalese sole, Solea senegalensis (Kaup)
Journal of fish diseases, 2010Co-Authors: I. Cano, Estefania J. Valverde, Benjamin Lopez-jimena, M.c. Alonso, Esther García-rosado, Carmen Sarasquete, Juan J. Borrego, Dolores CastroAbstract:6 páginas, 1 figura, 3 tablas.Lymphocystis is a viral disease with a worldwide geographical distribution that has been described in more than 125 species of teleost fish from marine and freshwater environments, affecting both wild and cultured fish (Anders 1989). However, in South Atlantic and Mediterranean mariculture, this disease affects gilthead seabream, Sparus aurata L., and Senegalese sole, Solea senegalensis (Kaup) (Menezes, Ramos & Pereira 1987; Le Deuff & Renault 1993; Borrego, Castro, Balebona, Garcia-Rosado & Lopez-Cortes 2001; Alonso, Cano, Garcia-Rosado, Castro, Lamas, Barja & Borrego 2005).This study has been supported by a CICYT project from the Spanish Government granted to Dr Sarasquete (AGL2006-17777-C03-02/ACU) and a project granted to Dr Borrego from Fundación Alfonso Martín Escudero.Peer reviewe
Alejandro Perretta - One of the best experts on this subject based on the ideXlab platform.
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Complete genome sequence and analysis of a novel Lymphocystivirus detected in whitemouth croaker (Micropogonias furnieri): lymphocystis disease virus 4
Archives of Virology, 2020Co-Authors: Andor Doszpoly, Rodrigo Puentes, Győző L Kajan, Alejandro PerrettaAbstract:A novel Lymphocystivirus causing typical signs of lymphocystis virus disease in whitemouth croaker ( Micropogonias furnieri ) on the coast of Uruguay was detected and described recently. Based on genetic analysis of some partially sequenced core genes, the virus seemed to differ from previously described members of the genus Lymphocystivirus . In this study, using next-generation sequencing, the whole genome of this virus was sequenced and analysed. The complete genome was found to be 211,086 bp in size, containing 148 predicted protein-coding regions, including the 26 core genes that seem to have a homologue in every iridovirus genome sequenced to date. Considering the current species demarcation criteria for the family Iridoviridae (genome organization, G+C content, amino acid sequence similarity, and phylogenetic relatedness of the core genes), the establishment of a novel species (“ Lymphocystis disease virus 4 ”) in the genus Lymphocystivirus is suggested.
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Diagnosis of lymphocystis disease in a novel host, the whitemouth croaker Micropogonias furnieri, associated with a putatively novel Lymphocystivirus species (LCDV-WC).
Diseases of aquatic organisms, 2020Co-Authors: Alejandro Perretta, Andor Doszpoly, R. Puentes, Martín BessonartAbstract:In 2015, an episode of lymphocystis disease (LCD) was detected in wild and cultured populations of whitemouth croaker Micropogonias furnieri off the coast of Uruguay. Fish of both origins were collected for histopathological and molecular investigations. Macroscopically, multinodular tumorlike masses were observed in the skin. Histological examination of these masses revealed enlarged cells with a hyaline capsule and basophilic inclusion bodies in the cytoplasm. The inclusion bodies were further examined by electron microscopy and showed icosahedral virions with a median diameter of 182 nm. Routine molecular investigations targeting the DNA polymerase and major capsid protein genes showed the presence of the DNA of an unknown lymphocystis disease virus (LCDV) in all specimens showing external signs of LCD. Subsequently, 4 other core genes were amplified and sequenced from the viral genome. Phylogenetic tree reconstruction based on the concatenated sequence of 6 core genes indicated that the virus undoubtedly belongs to the genus Lymphocystivirus. However, the core gene sequences of the whitemouth croaker LCDV differ markedly from those of the 3 known LCDVs, putatively representing a fourth LCDV species.
Alí Alejo - One of the best experts on this subject based on the ideXlab platform.
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An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus
Virology journal, 2013Co-Authors: Sergio M. Pontejo, Carolina Sánchez, Rocío Martín, Victoriano Mulero, Antonio Alcami, Alí AlejoAbstract:Background Lymphocystis disease virus (LCDV) is a large icosahedral dsDNA-containing virus of the Lymphocystivirus genus within the Iridoviridae family that can cause disease in more than 140 marine and freshwater fish species. While several isolates have been charcaterized and classified into distinct genotypes the complete genomic sequence is currently only available from two species, the LCDV-1, isolated from flounder (Platichtys flesus) in Europe and the LCDV-C, isolated from Japanese cultured flounder (Paralichthys olivaceus) in China. Analysis of the genome of LCDV-C showed it to encode a protein named LDVICp016 with similarities to the Tumour necrosis factor receptor (TNFR) superfamily with immunomodulatory potential.
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An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus
Virology Journal, 2013Co-Authors: Sergio M. Pontejo, Carolina Sánchez, Rocío Martín, Victoriano Mulero, Antonio Alcami, Alí AlejoAbstract:Background Lymphocystis disease virus (LCDV) is a large icosahedral dsDNA-containing virus of the Lymphocystivirus genus within the Iridoviridae family that can cause disease in more than 140 marine and freshwater fish species. While several isolates have been charcaterized and classified into distinct genotypes the complete genomic sequence is currently only available from two species, the LCDV-1, isolated from flounder ( Platichtys flesus ) in Europe and the LCDV-C, isolated from Japanese cultured flounder ( Paralichthys olivaceus ) in China. Analysis of the genome of LCDV-C showed it to encode a protein named LDVICp016 with similarities to the Tumour necrosis factor receptor (TNFR) superfamily with immunomodulatory potential. Findings We have expressed and purified the recombinant protein LDVICp016 and screened for potential interaction partners using surface plasmon resonance. Commercially available human and mouse members of the TNF superfamily (TNFSF), along with a representative set of fish-derived TNFSF were tested. We have found the LDVICp016 protein to be secreted and we have identified a second viral TNFR encoded by ORF 095 of the same virus. None of the 42 tested proteins were found to interact with LDVICp016. Conclusions We show that LDVICp016 is a secreted protein belonging to the TNF receptor family that may be part of a larger gene family in Lymphocystiviruses. While the ligand of this protein remains unknown, possibly due to the species specific nature of this interaction, further investigations into the potential role of this protein in the blockade of immune responses in its fish host are required.
Andor Doszpoly - One of the best experts on this subject based on the ideXlab platform.
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Complete genome sequence and analysis of a novel Lymphocystivirus detected in whitemouth croaker (Micropogonias furnieri): lymphocystis disease virus 4
Archives of Virology, 2020Co-Authors: Andor Doszpoly, Rodrigo Puentes, Győző L Kajan, Alejandro PerrettaAbstract:A novel Lymphocystivirus causing typical signs of lymphocystis virus disease in whitemouth croaker ( Micropogonias furnieri ) on the coast of Uruguay was detected and described recently. Based on genetic analysis of some partially sequenced core genes, the virus seemed to differ from previously described members of the genus Lymphocystivirus . In this study, using next-generation sequencing, the whole genome of this virus was sequenced and analysed. The complete genome was found to be 211,086 bp in size, containing 148 predicted protein-coding regions, including the 26 core genes that seem to have a homologue in every iridovirus genome sequenced to date. Considering the current species demarcation criteria for the family Iridoviridae (genome organization, G+C content, amino acid sequence similarity, and phylogenetic relatedness of the core genes), the establishment of a novel species (“ Lymphocystis disease virus 4 ”) in the genus Lymphocystivirus is suggested.
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Diagnosis of lymphocystis disease in a novel host, the whitemouth croaker Micropogonias furnieri, associated with a putatively novel Lymphocystivirus species (LCDV-WC).
Diseases of aquatic organisms, 2020Co-Authors: Alejandro Perretta, Andor Doszpoly, R. Puentes, Martín BessonartAbstract:In 2015, an episode of lymphocystis disease (LCD) was detected in wild and cultured populations of whitemouth croaker Micropogonias furnieri off the coast of Uruguay. Fish of both origins were collected for histopathological and molecular investigations. Macroscopically, multinodular tumorlike masses were observed in the skin. Histological examination of these masses revealed enlarged cells with a hyaline capsule and basophilic inclusion bodies in the cytoplasm. The inclusion bodies were further examined by electron microscopy and showed icosahedral virions with a median diameter of 182 nm. Routine molecular investigations targeting the DNA polymerase and major capsid protein genes showed the presence of the DNA of an unknown lymphocystis disease virus (LCDV) in all specimens showing external signs of LCD. Subsequently, 4 other core genes were amplified and sequenced from the viral genome. Phylogenetic tree reconstruction based on the concatenated sequence of 6 core genes indicated that the virus undoubtedly belongs to the genus Lymphocystivirus. However, the core gene sequences of the whitemouth croaker LCDV differ markedly from those of the 3 known LCDVs, putatively representing a fourth LCDV species.
Sergio M. Pontejo - One of the best experts on this subject based on the ideXlab platform.
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An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus
Virology journal, 2013Co-Authors: Sergio M. Pontejo, Carolina Sánchez, Rocío Martín, Victoriano Mulero, Antonio Alcami, Alí AlejoAbstract:Background Lymphocystis disease virus (LCDV) is a large icosahedral dsDNA-containing virus of the Lymphocystivirus genus within the Iridoviridae family that can cause disease in more than 140 marine and freshwater fish species. While several isolates have been charcaterized and classified into distinct genotypes the complete genomic sequence is currently only available from two species, the LCDV-1, isolated from flounder (Platichtys flesus) in Europe and the LCDV-C, isolated from Japanese cultured flounder (Paralichthys olivaceus) in China. Analysis of the genome of LCDV-C showed it to encode a protein named LDVICp016 with similarities to the Tumour necrosis factor receptor (TNFR) superfamily with immunomodulatory potential.
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An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus
Virology Journal, 2013Co-Authors: Sergio M. Pontejo, Carolina Sánchez, Rocío Martín, Victoriano Mulero, Antonio Alcami, Alí AlejoAbstract:Background Lymphocystis disease virus (LCDV) is a large icosahedral dsDNA-containing virus of the Lymphocystivirus genus within the Iridoviridae family that can cause disease in more than 140 marine and freshwater fish species. While several isolates have been charcaterized and classified into distinct genotypes the complete genomic sequence is currently only available from two species, the LCDV-1, isolated from flounder ( Platichtys flesus ) in Europe and the LCDV-C, isolated from Japanese cultured flounder ( Paralichthys olivaceus ) in China. Analysis of the genome of LCDV-C showed it to encode a protein named LDVICp016 with similarities to the Tumour necrosis factor receptor (TNFR) superfamily with immunomodulatory potential. Findings We have expressed and purified the recombinant protein LDVICp016 and screened for potential interaction partners using surface plasmon resonance. Commercially available human and mouse members of the TNF superfamily (TNFSF), along with a representative set of fish-derived TNFSF were tested. We have found the LDVICp016 protein to be secreted and we have identified a second viral TNFR encoded by ORF 095 of the same virus. None of the 42 tested proteins were found to interact with LDVICp016. Conclusions We show that LDVICp016 is a secreted protein belonging to the TNF receptor family that may be part of a larger gene family in Lymphocystiviruses. While the ligand of this protein remains unknown, possibly due to the species specific nature of this interaction, further investigations into the potential role of this protein in the blockade of immune responses in its fish host are required.
