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B. Cavari - One of the best experts on this subject based on the ideXlab platform.
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Ontogeny of somatolactin mRNA in the gilthead sea bream Sparus aurata
Fish Physiology and Biochemistry, 1997Co-Authors: B. Funkenstein, H. Kawauchi, B. CavariAbstract:Expression of somatolactin (SL) gene during embryonal and early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+)RNA was prepared from a pool of embryos collected at early and late stages or from larvae collected on different days after hatching. When hybridized to Sparus aurata SL cDNA, SL specific mRNA was seen both in embryos and in larvae. Levels of SL increased from day 1 onwards and reached the highest levels on day 21. Re-hybridization with Sparus aurata growth hormone (GH) cDNA revealed GH specific mRNA first on day 6 post-hatching. Levels of GH increased to maximal levels on day 10 and then decreased on days 15 and 21, thereby confirming the pattern of GH expression (Funkenstein and Cohen 1996). The patterns of SL and GH gene expression are different in gilthead sea bream during embryonal and larval development. Furthermore, the presence of SL transcript in embryos, prior to pituitary gland development, suggests the possibility that SL mRNA of maternal origin may be present in the oocyte.
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Isolation and characterization of somatolactin from pituitary glands of gilthead sea bream Sparus aurata
Aquaculture, 1995Co-Authors: B. Cavari, Toyohiko Noso, H. KawauchiAbstract:Abstract The complete amino acid sequence of somatolactin (SL) from the gilthead sea bream Sparus aurata has been determined. SL was isolated from the pituitary by alkaline extraction, gel filtration on a Sephadex G-100 column, and reversed-phase high-performance liquid chromatography on a TSK gel ODS-120T column. The purified protein was confirmed to be somatolactin by immunoblotting using chum salmon somatolactin antisera. Two forms of Sparus aurata SL exist; one (28 kDa) is probably glycosylated, while the other (25 kDa) is not and resembles SL found in Atlantic cod. SL consists of 207 amino acids that show remarkable conservation among fish SLs.
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Somatolactin, a novel pituitary protein: isolation and characterization from Sparus aurata.
Molecular marine biology and biotechnology, 1995Co-Authors: B. Cavari, Noso T, Kawauchi HAbstract:The complete amino acid sequence of somatolactin, a new pituitary protein belonging to the growth hormone/prolactin family, from the gilthead sea bream Sparus aurata has been determined. Somatolactin was isolated from the pituitary by alkaline extraction, gel filtration on a Sephadex G-100 column, and reversed-phase high-performance liquid chromatography (rpHPLC) on a TSK gel ODS-120T column. The purified protein was confirmed to be somatolactin by immunoblotting using chum salmon somatolactin antisera. It was found that Sparus aurata somatolactin consists of two forms; one form (28 kD) is probably a glycosylated form, while the other (25 kD) is a simple protein form, as was found also in Atlantic cod. The somatolactin consists of 207 amino acids that show remarkable conservation among fish somatolactins.
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Isolation of growth hormone and in vitro translation of mRNA isolated from pituitaries of the gilthead sea bream Sparus aurata
General and Comparative Endocrinology, 1994Co-Authors: B. Cavari, H. Kawauchi, Pierre-yves Le Bail, B. Levavi-sivan, P. Melamed, B. FunkensteinAbstract:Growth hormone (GH) polypeptide was purified from pituitary glands of the gilthead sea bream (Sparus aurata) by a two-step procedure involving gel filtration on Sephadex G-100 and reverse-phase high-performance liquid chromatography (rpHPLC). At each stage of purification, fractions were monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting using anti-bonito GH antiserum. The molecular weight of the sea bream GH was estimated by SDS-PAGE to be 21 kDa when electrophoresed in the absence of p-mercaptoethanol (nonreduced conditions) and 22 kDa when electrophoresed under reduced conditions (in the presence of 1% beta-mercaptoethanol). Pituitary RNA was used to direct cell-free translation. When specific immunoisolation from S-35-labeled proteins was conducted, using antisera against Sparus or tilapia GH, a larger prehormone was immunoprecipitated. The size of the pre-GH was estimated to be 27-28 kDa under reduced conditions and 26-27 kDa under nonreduced conditions, in agreement with the calculated molecular weight of Sparus pre-GH of 26,296 based on the deduced amino acid sequence of Sparus GH cDNA. The specificity of the immunoprecipitation reaction was demonstrated by the ability of recombinant tilapia GH to compete with the radioactively labeled translation product. No such competition was found after the addition of BSA. Our results demonstrate that the sea bream GH is similar in its size to other purified fish GHs and provide direct evidence for the synthesis of GH as a prepeptide, thus supporting the conclusions presented earlier by GH cDNA cloning.
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Isolation of growth hormone and in vitro translation of mRNA isolated from pituitaries of the gilthead sea bream Sparus aurata.
General and comparative endocrinology, 1994Co-Authors: B. Cavari, H. Kawauchi, Pierre-yves Le Bail, B. Levavi-sivan, P. Melamed, B. FunkensteinAbstract:Abstract Growth hormone (GH) polypeptide was purified from pituitary glands of the gilthead sea bream ( Sparus aurata ) by a two-step procedure involving gel filtration on Sephadex G-100 and reverse-phase high-performance liquid chromatography (rpHPLC). At each stage of purification, fractions were monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting using anti-bonito GH antiserum. The molecular weight of the sea bream GH was estimated by SDS-PAGE to be 21 kDa when electrophoresed in the absence of β-mercaptoethanol (nonreduced conditions) and 22 kDa when electrophoresed under reduced conditions (in the presence of 1% β-mercaptoethanol). Pituitary RNA was used to direct cell-free translation. When specific immunoisolation from 35 S-labeled proteins was conducted, using antisera against Sparus or tilapia GH, a larger prehormone was immunoprecipitated. The size of the pre-GH was estimated to be 27-28 kDa under reduced conditions and 26-27 kDa under nonreduced conditions, in agreement with the calculated molecular weight of Sparus pre-GH of 26,296 based on the deduced amino acid sequence of Sparus GH cDNA. The specificity of the immunoprecipitation reaction was demonstrated by the ability of recombinant tilapia GH to compete with the radioactively labeled translation product. No such competition was found after the addition of BSA. Our results demonstrate that the sea bream GH is similar in its size to other purified fish GHs and provide direct evidence for the synthesis of GH as a prepeptide, thus supporting the conclusions presented earlier by GH cDNA cloning.
B. Funkenstein - One of the best experts on this subject based on the ideXlab platform.
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Ontogeny of somatolactin mRNA in the gilthead sea bream Sparus aurata
Fish Physiology and Biochemistry, 1997Co-Authors: B. Funkenstein, H. Kawauchi, B. CavariAbstract:Expression of somatolactin (SL) gene during embryonal and early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+)RNA was prepared from a pool of embryos collected at early and late stages or from larvae collected on different days after hatching. When hybridized to Sparus aurata SL cDNA, SL specific mRNA was seen both in embryos and in larvae. Levels of SL increased from day 1 onwards and reached the highest levels on day 21. Re-hybridization with Sparus aurata growth hormone (GH) cDNA revealed GH specific mRNA first on day 6 post-hatching. Levels of GH increased to maximal levels on day 10 and then decreased on days 15 and 21, thereby confirming the pattern of GH expression (Funkenstein and Cohen 1996). The patterns of SL and GH gene expression are different in gilthead sea bream during embryonal and larval development. Furthermore, the presence of SL transcript in embryos, prior to pituitary gland development, suggests the possibility that SL mRNA of maternal origin may be present in the oocyte.
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Developmental and tissue-regulated expression of IGF-I and IGF-II mRNAs in Sparus aurata.
Journal of molecular endocrinology, 1996Co-Authors: S J Duguay, J Lai-zhang, Donald F. Steiner, B. Funkenstein, Shu Jin ChanAbstract:Recent studies have shown that homologues of the mammalian IGF-I and -II genes are also found in teleosts. We report here the cDNAs coding for IGF-I and IGF-II cloned from the gilthead seabream, Sparus aura ta. Sequence comparisons revealed that both IGFs have been well conserved among teleosts, although Sparus IGF-I is shorter bv three amino acid residues due to truncated B-and C-domains. Using the cloned cDNAs as probes, the relative expression of IGF-I and IGF-II mRNAs were assayed in different Sparus tissues. Sparus liver clearly contained the highest level of IGF-I mRNA while relatively high levels of IGF-II mRNA were found in liver, heart and gill using the ribonuclease protection assay. After GH administration the amount of IGF-I mRNA was increased by 220% in liver but no changes in IGF-II mRNA levels were detected in any tissue. We also assayed the expression of IGF-I and IGF-II in Sparus during early development. The IGF-II mRNA level was highest in larva I day after hatching and decreased thereafter. In contrast, IGF-I mRNA was detected in 1-day-old larva but there was an increase in expression in 12- and 16-day-old larva. These results demonstrated that the expression of IGF-I and IGF-II is highly regulated in teleosts and suggest that they play distinct roles during growth and development.
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Isolation of growth hormone and in vitro translation of mRNA isolated from pituitaries of the gilthead sea bream Sparus aurata
General and Comparative Endocrinology, 1994Co-Authors: B. Cavari, H. Kawauchi, Pierre-yves Le Bail, B. Levavi-sivan, P. Melamed, B. FunkensteinAbstract:Growth hormone (GH) polypeptide was purified from pituitary glands of the gilthead sea bream (Sparus aurata) by a two-step procedure involving gel filtration on Sephadex G-100 and reverse-phase high-performance liquid chromatography (rpHPLC). At each stage of purification, fractions were monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting using anti-bonito GH antiserum. The molecular weight of the sea bream GH was estimated by SDS-PAGE to be 21 kDa when electrophoresed in the absence of p-mercaptoethanol (nonreduced conditions) and 22 kDa when electrophoresed under reduced conditions (in the presence of 1% beta-mercaptoethanol). Pituitary RNA was used to direct cell-free translation. When specific immunoisolation from S-35-labeled proteins was conducted, using antisera against Sparus or tilapia GH, a larger prehormone was immunoprecipitated. The size of the pre-GH was estimated to be 27-28 kDa under reduced conditions and 26-27 kDa under nonreduced conditions, in agreement with the calculated molecular weight of Sparus pre-GH of 26,296 based on the deduced amino acid sequence of Sparus GH cDNA. The specificity of the immunoprecipitation reaction was demonstrated by the ability of recombinant tilapia GH to compete with the radioactively labeled translation product. No such competition was found after the addition of BSA. Our results demonstrate that the sea bream GH is similar in its size to other purified fish GHs and provide direct evidence for the synthesis of GH as a prepeptide, thus supporting the conclusions presented earlier by GH cDNA cloning.
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Isolation of growth hormone and in vitro translation of mRNA isolated from pituitaries of the gilthead sea bream Sparus aurata.
General and comparative endocrinology, 1994Co-Authors: B. Cavari, H. Kawauchi, Pierre-yves Le Bail, B. Levavi-sivan, P. Melamed, B. FunkensteinAbstract:Abstract Growth hormone (GH) polypeptide was purified from pituitary glands of the gilthead sea bream ( Sparus aurata ) by a two-step procedure involving gel filtration on Sephadex G-100 and reverse-phase high-performance liquid chromatography (rpHPLC). At each stage of purification, fractions were monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting using anti-bonito GH antiserum. The molecular weight of the sea bream GH was estimated by SDS-PAGE to be 21 kDa when electrophoresed in the absence of β-mercaptoethanol (nonreduced conditions) and 22 kDa when electrophoresed under reduced conditions (in the presence of 1% β-mercaptoethanol). Pituitary RNA was used to direct cell-free translation. When specific immunoisolation from 35 S-labeled proteins was conducted, using antisera against Sparus or tilapia GH, a larger prehormone was immunoprecipitated. The size of the pre-GH was estimated to be 27-28 kDa under reduced conditions and 26-27 kDa under nonreduced conditions, in agreement with the calculated molecular weight of Sparus pre-GH of 26,296 based on the deduced amino acid sequence of Sparus GH cDNA. The specificity of the immunoprecipitation reaction was demonstrated by the ability of recombinant tilapia GH to compete with the radioactively labeled translation product. No such competition was found after the addition of BSA. Our results demonstrate that the sea bream GH is similar in its size to other purified fish GHs and provide direct evidence for the synthesis of GH as a prepeptide, thus supporting the conclusions presented earlier by GH cDNA cloning.
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Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata.
Molecular and cellular endocrinology, 1992Co-Authors: B. Funkenstein, Amos Tandler, B. CavariAbstract:Abstract Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.
Pilar Alvarez-pellitero - One of the best experts on this subject based on the ideXlab platform.
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Ultrastructure and cytochemistry study of Goussia sparis (Protozoa: Apicomplexa) stages from the intestine of the gilthead sea bream Sparus aurata L. (Pisces: Teleostei)
Parasitology research, 1996Co-Authors: Pilar Alvarez-pellitero, Oswaldo Palenzuela, Ariadna Sitjà-bobadillaAbstract:The ultrastructure and cytochemistry of merogonial and gamogonial stages and early unsporulated oocysts of Goussia sparis in the intestine of Sparus aurata were studied. The typical pellicle was observed in some stages. The different stages might appear in intraepithelial or supraepithelial positions, but they were always intracellular. First steps of two apparently different endomerogonies were observed in intra- and supraepithelial positions, respectively. An apparent ectomerogony also occurred in supraepithelial stages. Developing macrogamonts showed surface invaginations and were densely packed with ribosomes, well-developed rough endoplasmic reticulum, mitochondria, amylopectin granules, lipidic droplets, and wall-forming-like bodies. The latter could participate in the formation of the oocyst wall. Abundant and large mitochondria, together with residual nuclei, appeared in advanced microgamonts. Microgametes showed two flagella with microtubul arranged according to the typical pattern. An increase in polysaccharide content was observed with coccidian development, reaching a maximum in zygotes and unsporulated oocysts.
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Light microscopic description of Eimeria sparis sp. nov. and Goussia sparis sp. nov. (Protozoa:Apicomplexa) from Sparus aurata L. (Pisces: Teleostei).
Parasitology research, 1996Co-Authors: Ariadna Sitjà-bobadilla, Oswaldo Palenzuela, Pilar Alvarez-pelliteroAbstract:Two new species of Coccidia, Eimeria sparis sp. nov. and Goussia sparis sp. nov., were found in the intestine of Sparus aurata from different culture systems of Spain. These are the first coccidian species described from this host. E. sparis is distinguished from other Eimeria spp. from Perciformes in the size and shape of the sporocysts, which measure 6–9.7 (mean=7.78, SD=1.33) ×4–6.5 (5.3±0.92) μm, and in the Stieda-like body. Mature oocysts, spherical or subspherical, measure 9.4–14.3 (11.88±1.85) μm. G. sparis differs from other Goussia spp. from fish of the Mediterranean area in the size and shape of the oocysts and sporocysts as well as in the location. Mature oocysts measure 16–21 (17.4±1.5)×13–18 (14.4±1.7) μm and sporocysts, 8.6–10.3 (9.5±0.5)× 5.7–7.4 (mean 6.5±0.5) μm. The different stages of merogony, gamogony, and sporogony of both species were examined at the light-microscope level in fresh material and histology specimens. Sporulation was endogenous in E. sparis and exogenous in G. sparis.
Ariadna Sitjà-bobadilla - One of the best experts on this subject based on the ideXlab platform.
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Enterospora nucleophila (Microsporidia) in Gilthead Sea Bream (Sparus aurata): Pathological Effects and Cellular Immune Response in Natural Infections.
Veterinary pathology, 2020Co-Authors: Amparo Picard-sánchez, Ariadna Sitjà-bobadilla, M. Carla Piazzon, Nahla Hossameldin Ahmed, Raquel Del Pozo, Oswaldo PalenzuelaAbstract:Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream (Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hin...
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Ultrastructure and cytochemistry study of Goussia sparis (Protozoa: Apicomplexa) stages from the intestine of the gilthead sea bream Sparus aurata L. (Pisces: Teleostei)
Parasitology research, 1996Co-Authors: Pilar Alvarez-pellitero, Oswaldo Palenzuela, Ariadna Sitjà-bobadillaAbstract:The ultrastructure and cytochemistry of merogonial and gamogonial stages and early unsporulated oocysts of Goussia sparis in the intestine of Sparus aurata were studied. The typical pellicle was observed in some stages. The different stages might appear in intraepithelial or supraepithelial positions, but they were always intracellular. First steps of two apparently different endomerogonies were observed in intra- and supraepithelial positions, respectively. An apparent ectomerogony also occurred in supraepithelial stages. Developing macrogamonts showed surface invaginations and were densely packed with ribosomes, well-developed rough endoplasmic reticulum, mitochondria, amylopectin granules, lipidic droplets, and wall-forming-like bodies. The latter could participate in the formation of the oocyst wall. Abundant and large mitochondria, together with residual nuclei, appeared in advanced microgamonts. Microgametes showed two flagella with microtubul arranged according to the typical pattern. An increase in polysaccharide content was observed with coccidian development, reaching a maximum in zygotes and unsporulated oocysts.
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Light microscopic description of Eimeria sparis sp. nov. and Goussia sparis sp. nov. (Protozoa:Apicomplexa) from Sparus aurata L. (Pisces: Teleostei).
Parasitology research, 1996Co-Authors: Ariadna Sitjà-bobadilla, Oswaldo Palenzuela, Pilar Alvarez-pelliteroAbstract:Two new species of Coccidia, Eimeria sparis sp. nov. and Goussia sparis sp. nov., were found in the intestine of Sparus aurata from different culture systems of Spain. These are the first coccidian species described from this host. E. sparis is distinguished from other Eimeria spp. from Perciformes in the size and shape of the sporocysts, which measure 6–9.7 (mean=7.78, SD=1.33) ×4–6.5 (5.3±0.92) μm, and in the Stieda-like body. Mature oocysts, spherical or subspherical, measure 9.4–14.3 (11.88±1.85) μm. G. sparis differs from other Goussia spp. from fish of the Mediterranean area in the size and shape of the oocysts and sporocysts as well as in the location. Mature oocysts measure 16–21 (17.4±1.5)×13–18 (14.4±1.7) μm and sporocysts, 8.6–10.3 (9.5±0.5)× 5.7–7.4 (mean 6.5±0.5) μm. The different stages of merogony, gamogony, and sporogony of both species were examined at the light-microscope level in fresh material and histology specimens. Sporulation was endogenous in E. sparis and exogenous in G. sparis.
Marc Carreras - One of the best experts on this subject based on the ideXlab platform.
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Testing Sparus II AUV, an open platform for industrial, scientific and academic applications
arXiv: Robotics, 2018Co-Authors: Marc Carreras, Eduard Vidal, Carles Candela, David Ribas, Narcis Palomeras, Angelos Mallios, Lluís Magií, Èric Pairet, Pere RidaoAbstract:This paper describes the experience of preparing and testing the Sparus II AUV in different applications. The AUV was designed as a lightweight vehicle combining the classical torpedo-shape features with the hovering capability. The robot has a payload area to allow the integration of different equipment depending on the application. The software architecture is based on ROS, an open framework that allows an easy integration of many devices and systems. Its flexibility, easy operation and openness makes the Sparus II AUV a multipurpose platform that can adapt to industrial, scientific and academic applications. Five units were developed in 2014, and different teams used and adapted the platform for different applications. The paper describes some of the experiences in preparing and testing this open platform to different applications.
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Testing Sparus II AUV, an open platform for industrial, scientific and academic applications
Instrumentation viewpoint, 2015Co-Authors: Marc Carreras, Eduard Vidal, Carles Candela, David Ribas, Narcis Palomeras, Lluís Magí, Angelos Mallios, Èric Vidal, Pere RidaoAbstract:This paper describes the experience of preparing and testing the Sparus II AUV in different applications. The AUV was designed as a lightweight vehicle combining the classical torpedo-shape features with the hovering capability. The robot has a payload area to allow the integration of different equipment depending on the devices and systems. Its flexibility, easy operation and openness makes the Sparus II AUV a multipurpose platform that can adapt to industrial, scientific and academic applications. Five units were developed in 2014, and different teams used and adapted the platform for different applications. The paper describes some of the experiences in preparing and testing this open platform to different applications.
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Online path planning for autonomous underwater vehicles in unknown environments
Proceedings - IEEE International Conference on Robotics and Automation, 2015Co-Authors: Juan David Hernández, Guillem Vallicrosa, Eduard Vidal, Enric Galceran, Marc CarrerasAbstract:— We present a framework for planning collision-free paths online for autonomous underwater vehicles (AUVs) in unknown environments. It is composed of three main modules (mapping, planning and mission handler) that incrementally explore the environment while solving start-to-goal queries. We use an octree-based representation of the environment and we extend the optimal rapidly-exploring random tree (RRT*) using concepts of anytime algorithms and lazy collision evaluation, thus including the capability to replan paths according to nearby obstacles perceived during the execution of the mission. To validate our approach, we plan paths for the Sparus-II AUV, a torpedo-shaped vehicle performing autonomous missions in a 2-dimensional workspace. We demonstrate its feasibility with the Sparus-II AUV in both simulation and real-world in-water trials.
