The Experts below are selected from a list of 1860 Experts worldwide ranked by ideXlab platform
Fumiya Obata - One of the best experts on this subject based on the ideXlab platform.
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age dependent and cell population restricted lrrk2 expression in normal mouse spleen
Biochemical and Biophysical Research Communications, 2010Co-Authors: Tatsunori Maekawa, Makoto Kubo, Ikue Yokoyama, Etsuro Ohta, Fumiya ObataAbstract:Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson's disease (PD), but its true physiological function remains unknown. In the normal mouse, LRRK2 is expressed in kidney, spleen, and lung at much higher levels than in brain, suggesting that LRRK2 may play an important role in these organs. Analysis of age-related changes in LRRK2 expression demonstrated that expression in kidney, lung, and various brain regions was constant throughout adult life. On the other hand, expression of both LRRK2 mRNA and protein decreased markedly in spleen in an age-dependent manner. Analysis of purified spleen cells indicated that B Lymphocytes were the major population expressing LRRK2, and that T Lymphocytes showed no expression. Consistently, the B Lymphocyte Surface Marker CD19 exhibited an age-dependent decrease of mRNA expression in spleen. These results suggest a possibly novel function of LRRK2 in the immune system, especially in B Lymphocytes.
Leonard C. Harrison - One of the best experts on this subject based on the ideXlab platform.
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T cell regulation mediated by interaction of soluble CD52 with the inhibitory receptor Siglec-10
Nature Immunology, 2013Co-Authors: Esther Bandala-sanchez, Yuxia Zhang, Simone Reinwald, James A. Dromey, Bo-han Lee, Junyan Qian, Ralph M. Böhmer, Leonard C. HarrisonAbstract:Functionally diverse T cell populations interact to maintain homeostasis of the immune system. We found that human and mouse antigen-activated T cells with high expression of the Lymphocyte Surface Marker CD52 suppressed other T cells. CD52(hi)CD4(+) T cells were distinct from CD4(+)CD25(+)Foxp3(+) regulatory T cells. Their suppression was mediated by soluble CD52 released by phospholipase C. Soluble CD52 bound to the inhibitory receptor Siglec-10 and impaired phosphorylation of the T cell receptor-associated kinases Lck and Zap70 and T cell activation. Humans with type 1 diabetes had a lower frequency and diminished function of CD52(hi)CD4(+) T cells responsive to the autoantigen GAD65. In diabetes-prone mice of the nonobese diabetic (NOD) strain, transfer of Lymphocyte populations depleted of CD52(hi) cells resulted in a substantially accelerated onset of diabetes. Our studies identify a ligand-receptor mechanism of T cell regulation that may protect humans and mice from autoimmune disease.
Yuzuru Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Lymphocyte Surface Marker genes in fugu.
Comparative biochemistry and physiology. Part D Genomics & proteomics, 2005Co-Authors: Hiroaki Suetake, Nil Ratan Saha, Kyosuke Araki, Kanako Akatsu, Kiyoshi Kikuchi, Yuzuru SuzukiAbstract:Abstract At present, much of the fish adaptive immune system remains unknown due to the paucity of Marker-specific reagents (e.g. antibodies) to identify immune cells. The genomic sequence of Takifugu rubripes (fugu) represents an important resource to facilitate the identification of Lymphocyte-related genes. Here, we review the recent works on B-Lymphocyte Markers, the heavy (H) chains of IgM and IgD, and Ig light chain, and T-cell Marker gene homologues, CD3e, CD3γ/δ, CD4, and CD8α in a single fish species, fugu. Expressions of these B- and T-Lymphocyte Markers homologues in peripheral blood leukocytes and other lymphoid tissues of fugu suggest that these molecules in fish would be available as Lymphocyte Markers. These findings will lead us to develop reliable reagents for the identification of Lymphocyte subpopulations. Fugu holds great promise as one of the model organisms for studies of development and evolution of adaptive and innate immunity in vertebrates.
Els N.t. Meeusen - One of the best experts on this subject based on the ideXlab platform.
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Lymphocyte Surface Marker and cytokine expression in peripheral and mucosal lymph nodes
Immunology, 1998Co-Authors: R. R. Premier, Els N.t. MeeusenAbstract:Lymphocyte populations, adhesion molecule and cytokine expression were determined in lymph nodes draining peripheral (popliteal and prescapular) or mucosal (abomasal and jejunal) tissue sites using flow cytometry analysis, immunostaining and cytokine-specific reverse-transcription-polymerase chain reaction (RT-PCR). Similar proportions of Lymphocyte subpopulations were present in all lymph nodes except for immunoglobulin A+ (IgA+) B cells which were only present in significant numbers in the gastrointestinal lymph nodes. Peripheral lymph nodes contained a significantly higher number of CD4+ cells expressing L-selectin and beta 1-integrin than mucosal lymph nodes while the alpha 4-integrin chain was expressed at similar levels in all lymph nodes. The peripheral node adressin recognized by the MECA 79 monoclonal antibody (mAb) was mainly expressed on peripheral lymph node vessels. RT-PCR analysis showed that interleukin (IL)-10 and IL-4 were preferentially induced in the gastrointestinal lymph nodes while IL-2 and interferon-gamma (IFN-gamma) were induced in all lymph nodes after polyclonal stimulation. These results indicate that there are substantial differences in the cell populations and microenvironments of lymph nodes draining mucosal and peripheral tissue sites in adult sheep.
Zhijian Cao - One of the best experts on this subject based on the ideXlab platform.
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St20, a new venomous animal derived natural peptide with immunosuppressive and anti-inflammatory activities.
Toxicon, 2017Co-Authors: Min Xiao, Li Ding, Weishan Yang, Lin Chai, Yuwen Sun, Xianyi Yang, Hua Zhang, Zhijian CaoAbstract:Peptide toxins from venomous animals are natural resources with diverse biological functions and therapeutic potential towards human diseases. For venomous scorpions, many valuable peptide toxins have been discovered from Buthidae scorpions, but few works were done about non-buthidae scorpions. Here, we cloned and characterized the first disulfide-bridged toxin peptide St20 from the non-buthidae scorpion Scorpiops tibetanus. St20 has a putative 23-residue signal peptide, followed by a presumed 34-residue mature peptide including 8 cysteines. Sequence alignments and structural analysis suggested that St20 is a new member of α-KTx23 scorpion toxin subfamily with a conserved CSα/β structural fold. Functional studies showed that St20 inhibited human T Lymphocyte Surface Marker CD69 expression and cytokine IL-2 secretion. Beside this, St20 inhibited two important pro-inflammatory factors TNF-α and IFN-γ secretion in the activated human T Lymphocyte. Animal experiments showed that the delayed-type hypersensitivity response in rat autoimmune disease model was ameliorated in the present of peptide toxin St20. Together, our results showed that St20 is the first disulfide-bridged toxin peptide from the non-buthidae scorpion Scorpiops tibetanus with immunosuppressive and anti-inflammatory activities, suggesting that toxins from non-buthidae scorpions might be a new source of peptide drug discovery towards human diseases.
