The Experts below are selected from a list of 5661 Experts worldwide ranked by ideXlab platform
Teigo Asai - One of the best experts on this subject based on the ideXlab platform.
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Correction: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina.
Organic & biomolecular chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:Correction for ‘Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina’ by Yohei Morishita et al., Org. Biomol. Chem., 2020, DOI: 10.1039/d0ob00519c.
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synthetic biology based discovery of a fungal macrolide from Macrophomina phaseolina
Organic and Biomolecular Chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.
Yohei Morishita - One of the best experts on this subject based on the ideXlab platform.
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Correction: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina.
Organic & biomolecular chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:Correction for ‘Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina’ by Yohei Morishita et al., Org. Biomol. Chem., 2020, DOI: 10.1039/d0ob00519c.
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synthetic biology based discovery of a fungal macrolide from Macrophomina phaseolina
Organic and Biomolecular Chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.
Makoto Fujita - One of the best experts on this subject based on the ideXlab platform.
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Correction: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina.
Organic & biomolecular chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:Correction for ‘Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina’ by Yohei Morishita et al., Org. Biomol. Chem., 2020, DOI: 10.1039/d0ob00519c.
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synthetic biology based discovery of a fungal macrolide from Macrophomina phaseolina
Organic and Biomolecular Chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.
Kiyohiro Adachi - One of the best experts on this subject based on the ideXlab platform.
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Correction: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina.
Organic & biomolecular chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:Correction for ‘Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina’ by Yohei Morishita et al., Org. Biomol. Chem., 2020, DOI: 10.1039/d0ob00519c.
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synthetic biology based discovery of a fungal macrolide from Macrophomina phaseolina
Organic and Biomolecular Chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.
Terutaka Sonohara - One of the best experts on this subject based on the ideXlab platform.
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Correction: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina.
Organic & biomolecular chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:Correction for ‘Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina’ by Yohei Morishita et al., Org. Biomol. Chem., 2020, DOI: 10.1039/d0ob00519c.
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synthetic biology based discovery of a fungal macrolide from Macrophomina phaseolina
Organic and Biomolecular Chemistry, 2020Co-Authors: Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo AsaiAbstract:A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.
