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Ludmila A. Golovleva - One of the best experts on this subject based on the ideXlab platform.
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Oxidase of the white Rot Fungus Panus tigrinus 8/18.
FEBS Letters, 1994Co-Authors: N N Pozdnyakova, Alexey A. Leontievsky, Ludmila A. GolovlevaAbstract:Extracellular oxidase of the white Rot Fungus Panus tigrinus earlier reported as laccase)contains copper but has no absorption spectrum typical of ‘blue’ oxidases. Thioglycolate and sodium azide inhibit the activity of this enzyme at concentrations 2.5–3 orders lower than those needed for fungal laccases. The oxidase of P. tigrinus oxidizes syringaldazine, coniferyl alcohol, ABTS, syringic acid, diaminobenzidine, guaiacol, catechol and vanillylacetone with different efficiencies. Oxygen consumption and no hydrogen peroxide formation were detected during substrate oxidation by P. tigrinus oxidase. It is proposed that P. tigrinus oxidase is a new ligninolytic enzyme.
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Production of ligninolytic enzymes of the white Rot Fungus Panus tigrinus
Journal of Biotechnology, 1994Co-Authors: Alexey A. Leontievsky, N. M. Myasoedova, Ludmila A. GolovlevaAbstract:Abstract We developed optimal conditions for submerged cultivation of the white Rot Fungus Panus tigrinus , which increase the yield of extracellular ligninolytic enzymes. Adding (besides mineral salts) Tween-80, an increased concentration of manganese, and 3-methylbenzyl alcohol as inducer into the medium and using an optimal source of carbon, optimally immobilized mycelium and an optimal temperature shift, we succeeded in obtaining 99 nkat ml −1 Mn-peroxidase by NADH oxidation and 18 nkat ml −1 oxidase by syringaldazine in batch cultivation in flasks and a 3-1 laboratory bioreactor.
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Ligninolytic enzymes of the white Rot Fungus Panus tigrinus
Biotechnology and Applied Biochemistry, 1991Co-Authors: O. V. Mal’tseva, Alexey A. Leontievsky, N. M. Myasoedova, Marja-leena Niku-paavola, Ludmila A. GolovlevaAbstract:The enzymes of the white Rot Fungus Panus tigrinus, synthesized during solid-state fermentation on straw, were purified and characterized. The enzymes are Mn-dependent peroxidase (MnP) (EC 1.11.1.−) and laccase (benzenediol:oxygen oxidoreductase; EC 1.10.3.2). The lignin peroxidase (LiP) (EC1.11.1.−) activity was not detected at any stage during cultivation. The M r of MnP was 43,000, and that of laccase 64,000. Isoelectric focusing resolved both preparations into two isoenzymes
Ryuichiro Kondo - One of the best experts on this subject based on the ideXlab platform.
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Fungal bioconversion of toxic polychlorinated biphenyls by white-Rot Fungus, Phlebia brevispora.
Applied Microbiology and Biotechnology, 2006Co-Authors: Ichiro Kamei, Shigenori Sonoki, Koichi Haraguchi, Ryuichiro KondoAbstract:Toxic coplanar polychlorinated biphenyls (Co-PCBs) were used as substrates for a degradation experiment with white-Rot Fungus, Phlebia brevispora TMIC33929, which is capable of degrading polychlorinated dibenzo-p-dioxins. Eleven PCB congener mixtures (7 mono-ortho- and 4 non-ortho-PCBs) were added to the cultures of P. brevispora and monitored by high resolution gas chromatography and mass spectrometry (HRGC/HRMS). Five PCB congeners, 3,3′,4,4′-tetrachlorobiphenyl, 2,3,3′,4,4′-pentachlorobiphenyl, 2,3′,4,4′,5-pentachlorobiphenyl, 3,3′,4,4′,5-pentachlorobiphenyl, and 2,3′,4,4′,5,5′-hexachlorobiphenyl were degraded by P. brevispora. To investigate the fungal metabolism of PCB, each Co-PCB was treated separately by P. brevispora and the metabolites were analyzed by gas chromatography and mass spectrometry (GC/MS) and identified on the basis of the GC/MS comparison with the authentic compound. Meta-methoxylated metabolite was detected from the culture containing each compound. Additionally, para-dechlorinated and -methoxylated metabolite was also detected from the culture with 2,3,3′,4,4′-pentachlorobiphenyl, 2,3′,4,4′,5-pentachlorobiphenyl, and 2,3′,4,4′,5,5′-hexachlorobiphenyl, which are mono-ortho-PCBs. In this paper, we identified the congener specific degradation of coplanar PCBs by P. brevispora, and clearly proved for the first time by identifying the metabolites that the white-Rot Fungus, P. brevispora, transformed recalcitrant coplanar PCBs.
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Oxidation of chlorinated dibenzo‐p‐dioxin and dibenzofuran by white‐Rot Fungus, Phlebia lindtneri
Fems Microbiology Letters, 2002Co-Authors: Toshio Mori, Ryuichiro KondoAbstract:The actions of a white-Rot Fungus on two chlorinated aromatic compounds, known to be persistent environmental contaminants, were studied. Two models, both-ring chlorinated dioxin, 2,7-dichlorodibenzo-p-dioxin (2,7-diCDD) and 2,8-dichlorodibenzofuran (2,8-diCDF), were metabolized by the white-Rot Fungus Phlebia lindtneri. 2,7-DiCDD disappeared linearly in the culture of P. lindtneri; over a 20-day incubation period, with only 45% remaining in the culture. One of the metabolites produced by P. lindtneri from a 5-day incubated culture with 2,7-diCDD or 2,8-diCDF was identified by gas chromatography–mass spectrometry. P. lindtneri was shown to metabolize 2,7-diCDD and 2,8-diCDF to hydroxy-diCDD and hydroxy-diCDF, respectively.
Per Olof Nyman - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a lignin peroxidase gene from the white-Rot Fungus Trametes versicolor
Biochimie, 1992Co-Authors: Leif J. Jönsson, Per Olof NymanAbstract:Abstract A genomic library of the white-Rot Fungus Trametes versicolor has been constructed and a gene coding for a lignin peroxidase has been isolated and sequenced. The gene, which contains 6 introns, encodes a pRotein of 346 amino acid residues, preceded by a tentative 26-residue signal peptide. The deduced amino-terminal sequence agrees with the amino-terminal end of a lignin peroxidase isozyme previously isolated from carbon-limited cultures of T versicolor.
Christian Mougin - One of the best experts on this subject based on the ideXlab platform.
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Expression of laccase IIIb from the white-Rot Fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications
2004Co-Authors: Claude Jolivalt, Catherine Madzak, Agathe Brault, Eliane Caminade, Christian Malosse, Christian MouginAbstract:Expression of laccase IIIb from the white-Rot Fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications. 2nd European Meeting in Oxizymes
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Biotransformation of the Herbicide Atrazine by the White Rot Fungus Phanerochaete chrysosporium.
Applied and Environmental Microbiology, 1994Co-Authors: Christian Mougin, Pierre Frasse, Michèle Asther, Chantal Laugero, Jacqueline DubrocaAbstract:Biotransformation of atrazine by the white Rot Fungus Phanerochaete chrysosporium was demonstrated by a 48% decrease of the initial herbicide concentration in the growth medium within the first 4 days of incubation, which corresponded to the mycelium-growing phase. Results clearly established the mineralization of the ethyl group of the herbicide. Analysis of the growth medium showed the formation of hydroxylated and/or N-dealkylated metabolites of atrazine during fungal degradation.
Yi Qian - One of the best experts on this subject based on the ideXlab platform.
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Decolorization of reactive brilliant red K-2BP by white Rot Fungus under sterile and non-sterile conditions
Journal of environmental sciences (China), 2006Co-Authors: Da-wen Gao, Xianghua Wen, Yi QianAbstract:Almost all the studies both domestic and international using white Rot Fungus for dye wastewater treatment are performed under sterile conditions. How ever, it is obviously unpractical that wastewater with dyes is treated under ste rile conditions. A feasible study was made for using white Rot Fungus Phanerocha ete chrysosporium to degrade reactive brilliant red K-2BP dye under non-sterile conditions. The results showed that there was no decolorizing effect under non-s terile condition if white Rot Fungus was incubated under non-sterile condition, and the decolorization was always near to 0% during decolorizing test for 3 d; i n the meantime, a lot of yeast Funguses were found in liquid medium when white r ot Fungus was incubated under non-sterile conditions; however, if white Rot fung us was incubated under sterile condition firstly, its decolorization was above 9 0% under non-sterile condition, which was similar to that of sterile condition. So we point out that the treating process for wastewater with dyes should be div ided into two stages. The first stage is that white Rot Fungus should be incubat ed under sterile conditions, and the second stage is that reactive brilliant red K-2BP is decolorized under non-sterile conditions. The method not only save the operation cost which decolorizing reactive brilliant red K-2BP under sterile co ndition, but also provide the feasibility for using white Rot Fungus to degrade wastewater with dyes under non-sterile conditions.
