The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Sudesh Kumar Yadav - One of the best experts on this subject based on the ideXlab platform.
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global de novo protein protein interactome elucidates interactions of drought responsive proteins in horse gram Macrotyloma Uniflorum
Journal of Proteome Research, 2016Co-Authors: Jyoti Bhardwaj, Indu Gangwar, Ganesh Panzade, Ravi Shankar, Sudesh Kumar YadavAbstract:Inspired by the availability of de novo transcriptome of horse gram (Macrotyloma Uniflorum) and recent developments in systems biology studies, the first ever global protein–protein interactome (PPI) map was constructed for this highly drought-tolerant legume. Large-scale studies of PPIs and the constructed database would provide rationale behind the interplay at cascading translational levels for drought stress-adaptive mechanisms in horse gram. Using a bidirectional approach (interolog and domain-based), a high-confidence interactome map and database for horse gram was constructed. Available transcriptomic information for shoot and root tissues of a sensitive (M-191; genotype 1) and a drought-tolerant (M-249; genotype 2) genotype of horse gram was utilized to draw comparative PPI subnetworks under drought stress. High-confidence 6804 interactions were predicted among 1812 proteins covering about one-fourth of the horse gram proteome. The highest number of interactions (33.86%) in horse gram interactome ...
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Overexpression of MuHSP70 gene from Macrotyloma Uniflorum confers multiple abiotic stress tolerance in transgenic Arabidopsis thaliana
Molecular Biology Reports, 2016Co-Authors: Shikha Masand, Sudesh Kumar YadavAbstract:A 70-KD heat shock protein (HSP70) is one of the most conserved chaperones. It is involved in de novo protein folding and prevents the aggregation of unfolded proteins under lethal environmental factors. The purpose of this study is to characterise a MuHSP70 from horsegram ( Macrotyloma Uniflorum ) and elucidating its role in stress tolerance of plants. A MuHSP70 was cloned and characterised from a natural drought stress tolerant HPK4 variety of horsegram ( M. Uniflorum ). For functional characterization, MuHSP70 was overexpressed in transgenic Arabidopsis. Overexpression of MuHSP70 was found to provide tolerance to the transgenic Arabidopsis against various stresses such as heat, cold, drought, salinity and oxidative stress. MuHSP70 transgenics were observed to maintain the shoot biomass, root length, relative water content, and chlorophyll content during exposure to multi-stresses relative to non-transgenic control. Transgenic lines have further shown the reduced levels of MDA, H_2O_2, and proteolytic activity. Together, these findings suggest that overexpression of MuHSP70 plays an important role in improving abiotic stress tolerance and could be a crucial candidate gene for exploration in crop improvement program.
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Global De Novo Protein–Protein Interactome Elucidates Interactions of Drought-Responsive Proteins in Horse Gram (Macrotyloma Uniflorum)
2016Co-Authors: Jyoti Bhardwaj, Indu Gangwar, Ganesh Panzade, Ravi Shankar, Sudesh Kumar YadavAbstract:Inspired by the availability of de novo transcriptome of horse gram (Macrotyloma Uniflorum) and recent developments in systems biology studies, the first ever global protein–protein interactome (PPI) map was constructed for this highly drought-tolerant legume. Large-scale studies of PPIs and the constructed database would provide rationale behind the interplay at cascading translational levels for drought stress-adaptive mechanisms in horse gram. Using a bidirectional approach (interolog and domain-based), a high-confidence interactome map and database for horse gram was constructed. Available transcriptomic information for shoot and root tissues of a sensitive (M-191; genotype 1) and a drought-tolerant (M-249; genotype 2) genotype of horse gram was utilized to draw comparative PPI subnetworks under drought stress. High-confidence 6804 interactions were predicted among 1812 proteins covering about one-fourth of the horse gram proteome. The highest number of interactions (33.86%) in horse gram interactome matched with Arabidopsis PPI data. The top five hub nodes mostly included ubiquitin and heat-shock-related proteins. Higher numbers of PPIs were found to be responsive in shoot tissue (416) and root tissue (2228) of genotype 2 compared with shoot tissue (136) and root tissue (579) of genotype 1. Characterization of PPIs using gene ontology analysis revealed that kinase and transferase activities involved in signal transduction, cellular processes, nucleocytoplasmic transport, protein ubiquitination, and localization of molecules were most responsive to drought stress. Hence, these could be framed in stress adaptive mechanisms of horse gram. Being the first legume global PPI map, it would provide new insights into gene and protein regulatory networks for drought stress tolerance mechanisms in horse gram. Information compiled in the form of database (MauPIR) will provide the much needed high-confidence systems biology information for horse gram genes, proteins, and involved processes. This information would ease the effort and increase the efficacy for similar studies on other legumes. Public access is available at http://14.139.59.221/MauPIR/
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comprehensive transcriptomic study on horse gram Macrotyloma Uniflorum de novo assembly functional characterization and comparative analysis in relation to drought stress
BMC Genomics, 2013Co-Authors: Jyoti Bhardwaj, Rakesh Kumar Chahota, Ravi Shankar, Rohit Chauhan, Mohit Kumar Swarnkar, Anil Kumar Singh, Sudesh Kumar YadavAbstract:Drought tolerance is an attribute maintained in plants by cross-talk between multiple and cascading metabolic pathways. Without a sequenced genome available for horse gram, it is difficult to comprehend such complex networks and intercalated genes associated with drought tolerance of horse gram (Macrotyloma Uniflorum). Therefore, de novo transcriptome discovery and associated analyses was done for this highly drought tolerant yet under exploited legume to decipher its genetic makeup. Eight samples comprising of shoot and root tissues of two horse gram genotypes (drought-sensitive; M-191 and drought-tolerant; M-249) were used for comparison under control and polyethylene glycol-induced drought stress conditions. Using Illumina sequencing technology, a total of 229,297,896 paired end read pairs were generated and utilized for de novo assembly of horse gram. Significant BLAST hits were obtained for 26,045 transcripts while, 3,558 transcripts had no hits but contained important conserved domains. A total of 21,887 unigenes were identified. SSRs containing sequences covered 16.25% of the transcriptome with predominant tri- and mono-nucleotides (43%). The total GC content of the transcriptome was found to be 43.44%. Under Gene Ontology response to stimulus, DNA binding and catalytic activity was highly expressed during drought stress conditions. Serine/threonine protein kinase was found to dominate in Enzyme Classification while pathways belonging to ribosome metabolism followed by plant pathogen interaction and plant hormone signal transduction were predominant in Kyoto Encyclopedia of Genes and Genomes analysis. Independent search on plant metabolic network pathways suggested valine degradation, gluconeogenesis and purine nucleotide degradation to be highly influenced under drought stress in horse gram. Transcription factors belonging to NAC, MYB-related, and WRKY families were found highly represented under drought stress. qRT-PCR validated the expression profile for 9 out of 10 genes analyzed in response to drought stress. De novo transcriptome discovery and analysis has generated enormous information over horse gram genomics. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against drought stress by horse gram. The knowledge generated can be further utilized for exploring other underexploited plants for stress responsive genes and improving plant tolerance.
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Comparative Analysis of DNA Methylation Polymorphism in Drought Sensitive (HPKC2) and Tolerant (HPK4) Genotypes of Horse Gram (Macrotyloma Uniflorum)
Biochemical Genetics, 2013Co-Authors: Jyoti Bhardwaj, Monika Mahajan, Sudesh Kumar YadavAbstract:DNA methylation is known as an epigenetic modification that affects gene expression in plants. Variation in CpG methylation behavior was studied in two natural horse gram ( Macrotyloma Uniflorum [Lam.] Verdc.) genotypes, HPKC2 (drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both genotypes was studied through methylation-sensitive amplified polymorphism. The results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4 (8.6%). Sequencing demonstrated sequence homology with the DRE binding factor (cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the polymorphic fragments showing alteration in methylation behavior. Differences in DNA methylation patterns could explain the differential drought tolerance and the epigenetic signature of these two horse gram genotypes.
T P Sastry - One of the best experts on this subject based on the ideXlab platform.
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effect of collagen sponge incorporating Macrotyloma Uniflorum extract on full thickness wound healing by down regulation of matrix metalloproteinases and inflammatory markers
RSC Advances, 2014Co-Authors: Thangavelu Muthukumar, Kannan Anbarasu, Dharmalingam Prakash, Baskar Santhosh Kumar, T P SastryAbstract:Collagen sponge (CS) was prepared using fish scales, which are a biological waste product in the marine food industry. CS was prepared so as to incorporate separately the drug mupirocin (thus designated CSM) and Macrotyloma Uniflorum plant extract (CSPE). CS, CSM and CSPE were applied to the experimental wounds of rats and the healing pattern was observed using various biological and physicochemical techniques. CSPE enhanced wound healing and was involved in the up-regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor β (TGF-β). Increased levels of hydroxyproline, hexosamine and uronic acid were observed in the CSPE-treated group compared with the other groups. Treatment with CSPE reduced inflammation, the expression of matrix metalloproteinases (MMPs) and scar formation, thereby contributing to faster wound healing.
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effect of growth factors and pro inflammatory cytokines by the collagen biocomposite dressing material containing Macrotyloma Uniflorum plant extract in vivo wound healing
Colloids and Surfaces B: Biointerfaces, 2014Co-Authors: Thangavelu Muthukumar, Kannan Anbarasu, Dharmalingam Prakash, T P SastryAbstract:Abstract Open burn wounds require proper dressings for faster healing and to prevent infection. In the present study, a wound dressing material in sheet form, containing fish scale collagen (FSC), physiologically clotted fibrin (PCF) and Macrotyloma Uniflorum plant extract (MPE) was applied on the experimental wounds of rats. It was found that MPE accelerated wound healing, by suppressing the cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) expressions thereby reduced inflammation. It has influenced the regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor (TGF-β). The biocomposite sheet has enhanced collagen synthesis and down regulated the matrix metalloproteinases (MMPs), thereby helped faster healing of wounds. The results suggest that the incorporation of MPE played an important role in enhancing the wound healing rate. The FSC–PCF biosheet containing MPE may be further tried on the clinical wounds of small and large animals before application on to humans.
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fish scale collagen sponge incorporated with Macrotyloma Uniflorum plant extract as a possible wound burn dressing material
Colloids and Surfaces B: Biointerfaces, 2014Co-Authors: Thangavelu Muthukumar, P Prabu, Kausik Ghosh, T P SastryAbstract:Application of plant extracts for the burn/wound treatment is followed over the decades as a common practice and it is an important aspect in clinical management. In this study porous collagen sponges (CS) were prepared using fish scales and were incorporated with mupirocin (CSM) and extracts of Macrotyloma Uniflorum (CSPE) separately to impart antimicrobial activity to the sponges. The results showed that the addition of plant extract increased the tensile strength of CSPE and stability against collagenase enzyme. FTIR studies have shown the incorporation of plant extract in CSPE, SEM studies have revealed the porous nature of the sponges and XRD patterns have shown the retention of collagen triple helical structure even after the addition of plant extract. CSPE and CSM have exhibited antimicrobial properties. The sponges prepared were analysed for their in vitro biocompatibility studies using fibroblasts and keratinocyte cell lines and the results have shown their biocompatible nature. Based on the results obtained, CS, CSM and CSPE may be tried as a burn/wound dressing materials, initially, in small animals in vivo.
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synthesis and characterization of biosheet impregnated with Macrotyloma Uniflorum extract for burn wound dressings
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Thangavelu Muthukumar, Rethinam Senthil, T P SastryAbstract:Developing biomaterials having wound healing properties within the search of a common man is the need of hour, particularly in developing and third world countries. Keeping this objective in view we have developed a wound dressing material, in sheet form, containing fish scale collagen (FSC) and physiologically clotted fibrin (PCF), both are by products of aqua food and meat industries respectively. To impart antimicrobial properties to the composite sheet, it was incorporated with Macrotyloma Uniflorum plant extract (MPE). SEM pictures have shown that FSC:PCF:MPE composite has fibrous and porous surface which helps in transportation of oxygen as well as absorbing wound fluids and their evaporation. The biomaterials have shown 100% biocompatibility and the percentage cell viability was found to be above 89%. The FSC:PCF:MPE biocomposite film with required mechanical strength, biocompatibility and antimicrobial properties can be tried as a burn/wound dressing material.
Jyoti Bhardwaj - One of the best experts on this subject based on the ideXlab platform.
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global de novo protein protein interactome elucidates interactions of drought responsive proteins in horse gram Macrotyloma Uniflorum
Journal of Proteome Research, 2016Co-Authors: Jyoti Bhardwaj, Indu Gangwar, Ganesh Panzade, Ravi Shankar, Sudesh Kumar YadavAbstract:Inspired by the availability of de novo transcriptome of horse gram (Macrotyloma Uniflorum) and recent developments in systems biology studies, the first ever global protein–protein interactome (PPI) map was constructed for this highly drought-tolerant legume. Large-scale studies of PPIs and the constructed database would provide rationale behind the interplay at cascading translational levels for drought stress-adaptive mechanisms in horse gram. Using a bidirectional approach (interolog and domain-based), a high-confidence interactome map and database for horse gram was constructed. Available transcriptomic information for shoot and root tissues of a sensitive (M-191; genotype 1) and a drought-tolerant (M-249; genotype 2) genotype of horse gram was utilized to draw comparative PPI subnetworks under drought stress. High-confidence 6804 interactions were predicted among 1812 proteins covering about one-fourth of the horse gram proteome. The highest number of interactions (33.86%) in horse gram interactome ...
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Global De Novo Protein–Protein Interactome Elucidates Interactions of Drought-Responsive Proteins in Horse Gram (Macrotyloma Uniflorum)
2016Co-Authors: Jyoti Bhardwaj, Indu Gangwar, Ganesh Panzade, Ravi Shankar, Sudesh Kumar YadavAbstract:Inspired by the availability of de novo transcriptome of horse gram (Macrotyloma Uniflorum) and recent developments in systems biology studies, the first ever global protein–protein interactome (PPI) map was constructed for this highly drought-tolerant legume. Large-scale studies of PPIs and the constructed database would provide rationale behind the interplay at cascading translational levels for drought stress-adaptive mechanisms in horse gram. Using a bidirectional approach (interolog and domain-based), a high-confidence interactome map and database for horse gram was constructed. Available transcriptomic information for shoot and root tissues of a sensitive (M-191; genotype 1) and a drought-tolerant (M-249; genotype 2) genotype of horse gram was utilized to draw comparative PPI subnetworks under drought stress. High-confidence 6804 interactions were predicted among 1812 proteins covering about one-fourth of the horse gram proteome. The highest number of interactions (33.86%) in horse gram interactome matched with Arabidopsis PPI data. The top five hub nodes mostly included ubiquitin and heat-shock-related proteins. Higher numbers of PPIs were found to be responsive in shoot tissue (416) and root tissue (2228) of genotype 2 compared with shoot tissue (136) and root tissue (579) of genotype 1. Characterization of PPIs using gene ontology analysis revealed that kinase and transferase activities involved in signal transduction, cellular processes, nucleocytoplasmic transport, protein ubiquitination, and localization of molecules were most responsive to drought stress. Hence, these could be framed in stress adaptive mechanisms of horse gram. Being the first legume global PPI map, it would provide new insights into gene and protein regulatory networks for drought stress tolerance mechanisms in horse gram. Information compiled in the form of database (MauPIR) will provide the much needed high-confidence systems biology information for horse gram genes, proteins, and involved processes. This information would ease the effort and increase the efficacy for similar studies on other legumes. Public access is available at http://14.139.59.221/MauPIR/
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comprehensive transcriptomic study on horse gram Macrotyloma Uniflorum de novo assembly functional characterization and comparative analysis in relation to drought stress
BMC Genomics, 2013Co-Authors: Jyoti Bhardwaj, Rakesh Kumar Chahota, Ravi Shankar, Rohit Chauhan, Mohit Kumar Swarnkar, Anil Kumar Singh, Sudesh Kumar YadavAbstract:Drought tolerance is an attribute maintained in plants by cross-talk between multiple and cascading metabolic pathways. Without a sequenced genome available for horse gram, it is difficult to comprehend such complex networks and intercalated genes associated with drought tolerance of horse gram (Macrotyloma Uniflorum). Therefore, de novo transcriptome discovery and associated analyses was done for this highly drought tolerant yet under exploited legume to decipher its genetic makeup. Eight samples comprising of shoot and root tissues of two horse gram genotypes (drought-sensitive; M-191 and drought-tolerant; M-249) were used for comparison under control and polyethylene glycol-induced drought stress conditions. Using Illumina sequencing technology, a total of 229,297,896 paired end read pairs were generated and utilized for de novo assembly of horse gram. Significant BLAST hits were obtained for 26,045 transcripts while, 3,558 transcripts had no hits but contained important conserved domains. A total of 21,887 unigenes were identified. SSRs containing sequences covered 16.25% of the transcriptome with predominant tri- and mono-nucleotides (43%). The total GC content of the transcriptome was found to be 43.44%. Under Gene Ontology response to stimulus, DNA binding and catalytic activity was highly expressed during drought stress conditions. Serine/threonine protein kinase was found to dominate in Enzyme Classification while pathways belonging to ribosome metabolism followed by plant pathogen interaction and plant hormone signal transduction were predominant in Kyoto Encyclopedia of Genes and Genomes analysis. Independent search on plant metabolic network pathways suggested valine degradation, gluconeogenesis and purine nucleotide degradation to be highly influenced under drought stress in horse gram. Transcription factors belonging to NAC, MYB-related, and WRKY families were found highly represented under drought stress. qRT-PCR validated the expression profile for 9 out of 10 genes analyzed in response to drought stress. De novo transcriptome discovery and analysis has generated enormous information over horse gram genomics. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against drought stress by horse gram. The knowledge generated can be further utilized for exploring other underexploited plants for stress responsive genes and improving plant tolerance.
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Comparative Analysis of DNA Methylation Polymorphism in Drought Sensitive (HPKC2) and Tolerant (HPK4) Genotypes of Horse Gram (Macrotyloma Uniflorum)
Biochemical Genetics, 2013Co-Authors: Jyoti Bhardwaj, Monika Mahajan, Sudesh Kumar YadavAbstract:DNA methylation is known as an epigenetic modification that affects gene expression in plants. Variation in CpG methylation behavior was studied in two natural horse gram ( Macrotyloma Uniflorum [Lam.] Verdc.) genotypes, HPKC2 (drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both genotypes was studied through methylation-sensitive amplified polymorphism. The results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4 (8.6%). Sequencing demonstrated sequence homology with the DRE binding factor (cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the polymorphic fragments showing alteration in methylation behavior. Differences in DNA methylation patterns could explain the differential drought tolerance and the epigenetic signature of these two horse gram genotypes.
Zafar Alam Mahmood - One of the best experts on this subject based on the ideXlab platform.
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near infra red spectroscopic analysis of Macrotyloma Uniflorum lam verdc phaseolus lunatus linn a nd phaseolus vulgaris linn
Journal of Pharmaceutical and Scientific Innovation, 2016Co-Authors: Salman Ahmed, Muhammad Mohtasheemul Hasan, Rafi Akhtar Sultan, Zafar Alam MahmoodAbstract:The present study is designed to determine the types of se condary metabolites in three selected seed powders by fast, reliable , and non - destructive Near Infra Red spectroscopic analytical technique. NIRS analysis of Macrotyloma Uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., and Phaseolus vulgaris Linn., seed f lours were done for qualitative evaluation of secondary metabolites. The fine powder of crude drug was directly applied in FOSS XDS near infrared Rapid Content Analyzer. The properly filled powdered sample in a disposable glass silicate vial and closed wit h plastic lid was centrally placed onto the sample presentation glass of Foss Rapid Content Analyzer. The scanning of sample at ambient tempera t ure gives NIR spectra within 30 seconds . The major proposed compounds in M. Uniflorum were 3,4 - dihydroxybenzoic acid, caffeic acid, chlorogenic acid, ferulic acid, gallic acid, kaempferol, linolenic acid, niacin, quercetin, sinapinic acid, syringic acid , and vanillic acid. Whereas, coumaric acid, fructose, galactose, L - serine, raffinose, stachyose, sucrose, threonin e, and tyrosine were detected in P. lunatus and the NIR suggested the presence of caffeic acid, ferulic acid, sinapinic acid, phytic acid, leucine, and valine in P. vulgaris. NIRS a s an advance innovative technology extensively used in chemical, food, petr ochemical , and pharmaceutical industries for rapid and reliable standardization . The present study revealed a good correlation to conclude that flour samples of all the three legume seeds could potentially be added to food system to achieve nutritional an d functional benefits.
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inhibition of calcium oxalate crystals growth by Macrotyloma Uniflorum lam verdc phaseolus lunatus linn and phaseolus vulgaris linn an in vitro study
Journal of Pharmacognosy and Phytochemistry, 2016Co-Authors: Salman Ahmed, Muhammad Mohtasheemul Hasan, Zafar Alam MahmoodAbstract:Purpose: The purpose of the study is to explore all possible morphological features of calcium oxalate monohydrate and calcium oxalate dihydrate crystals and their habits in case of inhibition.rnMethods: The study was carried out on glass slide to observe the growth and inhibition of calcium oxalate monohydrate (COM) crystals by using infusions (5-20%) of Macrotyloma Uniflorum (Lam.) Verdc, Phaseolus lunatus Linn. and Phaseolus vulgaris Linn. The reagents of double diffusion gel technique was used for this purpose. Results: Calcium oxalate crystals are divided into three types, calcium oxalate monohydrate, calcium oxalate dihydrate and calcium oxalate trihydrate. These types are further divided into sub types on the basis of their morphology. In case of calcium oxalate monohydrate (COM), these crystals are donut, dumbbell, needles, platy, prismatic, rosette, round edges and X-shaped. Whereas, calcium oxalate dihydrate (COD) are reported as the elongated large rods and tetragonal bipyramidal forms. In the present study dendritic or arborescent (tree like platy crystals) were observed for the first time as the part of a COM growth. Long chain loose agglomerates and compact aggregated crystals are the common pattern of calcium oxalate crystals. All tested infusions caused growth inhibition of calcium oxalate crystals. Smaller zones of nucleation and defected shape of the grown crystals; declare as different patterns of growth inhibition. Conclusion: This study gives an extensive information about morphology, aggregation and growth inhibition of calcium oxalate crystals.
Thangavelu Muthukumar - One of the best experts on this subject based on the ideXlab platform.
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effect of collagen sponge incorporating Macrotyloma Uniflorum extract on full thickness wound healing by down regulation of matrix metalloproteinases and inflammatory markers
RSC Advances, 2014Co-Authors: Thangavelu Muthukumar, Kannan Anbarasu, Dharmalingam Prakash, Baskar Santhosh Kumar, T P SastryAbstract:Collagen sponge (CS) was prepared using fish scales, which are a biological waste product in the marine food industry. CS was prepared so as to incorporate separately the drug mupirocin (thus designated CSM) and Macrotyloma Uniflorum plant extract (CSPE). CS, CSM and CSPE were applied to the experimental wounds of rats and the healing pattern was observed using various biological and physicochemical techniques. CSPE enhanced wound healing and was involved in the up-regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor β (TGF-β). Increased levels of hydroxyproline, hexosamine and uronic acid were observed in the CSPE-treated group compared with the other groups. Treatment with CSPE reduced inflammation, the expression of matrix metalloproteinases (MMPs) and scar formation, thereby contributing to faster wound healing.
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effect of growth factors and pro inflammatory cytokines by the collagen biocomposite dressing material containing Macrotyloma Uniflorum plant extract in vivo wound healing
Colloids and Surfaces B: Biointerfaces, 2014Co-Authors: Thangavelu Muthukumar, Kannan Anbarasu, Dharmalingam Prakash, T P SastryAbstract:Abstract Open burn wounds require proper dressings for faster healing and to prevent infection. In the present study, a wound dressing material in sheet form, containing fish scale collagen (FSC), physiologically clotted fibrin (PCF) and Macrotyloma Uniflorum plant extract (MPE) was applied on the experimental wounds of rats. It was found that MPE accelerated wound healing, by suppressing the cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) expressions thereby reduced inflammation. It has influenced the regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor (TGF-β). The biocomposite sheet has enhanced collagen synthesis and down regulated the matrix metalloproteinases (MMPs), thereby helped faster healing of wounds. The results suggest that the incorporation of MPE played an important role in enhancing the wound healing rate. The FSC–PCF biosheet containing MPE may be further tried on the clinical wounds of small and large animals before application on to humans.
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Collagen Scaffold Incorporated with Macrotyloma Uniflorum Plant Extracts as a–Burn/Wound Dressing Material, in Vitro and in Vivo Evaluation
2014Co-Authors: Thangavelu Muthukumar, Thotapalli Parvathaleswara SastryAbstract:Collagen is the most abundantly available connective tissue protein, which is being used as a biomaterial for various biomedical applications. Presently, fish wastes are disposed improperly which is causing serious environmental pollution resulting in offensive odour. Fish scales are promising source of Type I collagen. Medicinal plants have been used since time immemorial for treatment of various ailments of skin and dermatological disorders especially cuts, wounds, and burns. Developing biomaterials from the natural sources which are having wound healing properties within the search of a common man is the need of hour, particularly in developing and third world countries. With these objectives in view we have developed a wound dressing material containing fish scale collagen (FSC) incorporated with Macrotyloma Uniflorum plant extract (PE). The wound dressing composite was characterized for its physiochemical properties using conventional methods. SEM image revealed that the composite has fibrous and porous surface which helps in transportation of oxygen as well as absorbing wound fluids. The biomaterial has shown 95% biocompatibility with required mechanical strength and has exhibited antimicrobial properties. This biomaterial has been used as a wound dressing material in experimental wounds of rats. The healing pattern was evaluated by macroscopic observations, panimetric studies, biochemical, histopathological observations. The results showed faster healing pattern in the wounds treated with CSPE compared to the other composites used in this study and untreated control. These experiments clearly suggest that CSPE can be used as wound/burn dressing materials
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fish scale collagen sponge incorporated with Macrotyloma Uniflorum plant extract as a possible wound burn dressing material
Colloids and Surfaces B: Biointerfaces, 2014Co-Authors: Thangavelu Muthukumar, P Prabu, Kausik Ghosh, T P SastryAbstract:Application of plant extracts for the burn/wound treatment is followed over the decades as a common practice and it is an important aspect in clinical management. In this study porous collagen sponges (CS) were prepared using fish scales and were incorporated with mupirocin (CSM) and extracts of Macrotyloma Uniflorum (CSPE) separately to impart antimicrobial activity to the sponges. The results showed that the addition of plant extract increased the tensile strength of CSPE and stability against collagenase enzyme. FTIR studies have shown the incorporation of plant extract in CSPE, SEM studies have revealed the porous nature of the sponges and XRD patterns have shown the retention of collagen triple helical structure even after the addition of plant extract. CSPE and CSM have exhibited antimicrobial properties. The sponges prepared were analysed for their in vitro biocompatibility studies using fibroblasts and keratinocyte cell lines and the results have shown their biocompatible nature. Based on the results obtained, CS, CSM and CSPE may be tried as a burn/wound dressing materials, initially, in small animals in vivo.
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synthesis and characterization of biosheet impregnated with Macrotyloma Uniflorum extract for burn wound dressings
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Thangavelu Muthukumar, Rethinam Senthil, T P SastryAbstract:Developing biomaterials having wound healing properties within the search of a common man is the need of hour, particularly in developing and third world countries. Keeping this objective in view we have developed a wound dressing material, in sheet form, containing fish scale collagen (FSC) and physiologically clotted fibrin (PCF), both are by products of aqua food and meat industries respectively. To impart antimicrobial properties to the composite sheet, it was incorporated with Macrotyloma Uniflorum plant extract (MPE). SEM pictures have shown that FSC:PCF:MPE composite has fibrous and porous surface which helps in transportation of oxygen as well as absorbing wound fluids and their evaporation. The biomaterials have shown 100% biocompatibility and the percentage cell viability was found to be above 89%. The FSC:PCF:MPE biocomposite film with required mechanical strength, biocompatibility and antimicrobial properties can be tried as a burn/wound dressing material.
